Assuntos
Sequestro Broncopulmonar/diagnóstico por imagem , Diafragma/diagnóstico por imagem , Biomarcadores/metabolismo , Sequestro Broncopulmonar/diagnóstico , Sequestro Broncopulmonar/enzimologia , Diagnóstico Diferencial , Diafragma/patologia , Humanos , Lactente , Masculino , Fosfopiruvato Hidratase/metabolismo , UltrassonografiaRESUMO
We addressed the in vivo role of phosphatidylinositol 3-kinase-gamma (PI3K-gamma) in signaling the sequestration of polymorphonuclear leukocytes (PMNs) in lungs and in the mechanism of inflammatory lung vascular injury. We studied mice with deletion of the p110 catalytic subunit of PI3K-gamma (PI3K-gamma(-/-) mice). We measured lung tissue PMN sequestration, microvascular permeability, and edema formation after bacteremia induced by intraperitoneal Escherichia coli challenge. PMN infiltration into the lung interstitium in PI3K-gamma(-/-) mice as assessed morphometrically was increased 100% over that in control mice within 1 h after bacterial challenge. PI3K-gamma(-/-) mice also developed a greater increase in lung microvascular permeability after E. coli challenge, resulting in edema formation. The augmented lung tissue PMN sequestration in PI3K-gamma(-/-) mice was associated with increased expression of the PMN adhesive proteins CD47 and beta(3)-integrins. We observed increased association of CD47 and beta(3)-integrins with the extracellular matrix protein vitronectin in lungs of PI3K-gamma(-/-) mice after E. coli challenge. PMNs from these mice also showed increased beta(3)-integrin expression and augmented beta(3)-integrin-dependent PMN adhesion to vitronectin. These results point to a key role of PMN PI3K-gamma in negatively regulating CD47 and beta(3)-integrin expression in gram-negative sepsis. PI3K-gamma activation in PMNs induced by E. coli may modulate the extent of lung tissue PMN sequestration secondary to CD47 and beta(3)-integrin expression. Therefore, the level of PI3K-gamma activation may be an important determinant of PMN-dependent lung vascular injury.
Assuntos
Infecções por Escherichia coli/enzimologia , Escherichia coli , Infiltração de Neutrófilos , Neutrófilos/enzimologia , Fosfatidilinositol 3-Quinases/metabolismo , Sepse/enzimologia , Animais , Vasos Sanguíneos/enzimologia , Vasos Sanguíneos/lesões , Vasos Sanguíneos/patologia , Sequestro Broncopulmonar/enzimologia , Sequestro Broncopulmonar/genética , Sequestro Broncopulmonar/microbiologia , Sequestro Broncopulmonar/patologia , Antígeno CD47/metabolismo , Permeabilidade Capilar/genética , Adesão Celular/genética , Classe Ib de Fosfatidilinositol 3-Quinase , Infecções por Escherichia coli/complicações , Infecções por Escherichia coli/genética , Infecções por Escherichia coli/patologia , Regulação da Expressão Gênica/genética , Integrina beta3/metabolismo , Isoenzimas/deficiência , Isoenzimas/genética , Isoenzimas/metabolismo , Pulmão/enzimologia , Pulmão/microbiologia , Pulmão/patologia , Camundongos , Camundongos Knockout , Infiltração de Neutrófilos/genética , Neutrófilos/patologia , Fosfatidilinositol 3-Quinases/deficiência , Fosfatidilinositol 3-Quinases/genética , Sepse/genética , Sepse/microbiologia , Sepse/patologia , Transdução de Sinais/genéticaRESUMO
Nitric oxide (NO) derived from inducible NO synthase (iNOS) contributes to the pathophysiology of acute lung injury (ALI). The effect of iNOS on pulmonary neutrophil infiltration in ALI is not known. Thus, we assessed pulmonary microvascular neutrophil sequestration through intravital videomicroscopy and pulmonary neutrophil infiltration, reflected by myeloperoxidase activity and lavage neutrophil counts, after induction of sepsis by cecal ligation/perforation in wild-type (iNOS+/+) versus iNOS-/- mice. Pulmonary microvascular neutrophil sequestration was attenuated in septic iNOS-/- versus iNOS+/+ mice (15 +/- 1 vs. 20 +/- 1 leukocytes per field, p < 0.05), but lavage neutrophil counts were greater in iNOS-/- mice (5.7 +/- 1.5% vs. 0.7 +/- 0.1%, p < 0.05) between 6 and 18 hours after cecal ligation and perforation. When iNOS+/+ bone marrow was transplanted into bone marrow-depleted iNOS-/- mice (+ to - chimeras; iNOS limited to marrow-derived inflammatory cells), septic pulmonary microvascular neutrophil sequestration and lavage neutrophil counts were restored to levels seen in septic iNOS+/+ mice. In contrast, in - to + chimeras, pulmonary neutrophil trafficking was similar to iNOS-/- mice. In vitro cytokine-stimulated neutrophil transendothelial migration was significantly greater for iNOS-/- versus iNOS+/+ neutrophils (7.9 +/- 0.7% vs. 3.8 +/- 0.6%, p < 0.05) but was independent of endothelial iNOS. Thus, neutrophil iNOS-derived NO is an important autocrine modulator of pulmonary neutrophil infiltration in murine sepsis.
Assuntos
Pneumopatias/fisiopatologia , Infiltração de Neutrófilos/imunologia , Óxido Nítrico Sintase/metabolismo , Síndrome de Resposta Inflamatória Sistêmica/fisiopatologia , Doença Aguda , Animais , Sequestro Broncopulmonar/enzimologia , Sequestro Broncopulmonar/imunologia , Movimento Celular/imunologia , Modelos Animais de Doenças , Endotélio Vascular/metabolismo , Pneumopatias/complicações , Pneumopatias/patologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Óxido Nítrico/biossíntese , Óxido Nítrico/imunologia , Óxido Nítrico Sintase/deficiência , Distribuição Aleatória , Síndrome de Resposta Inflamatória Sistêmica/complicações , Síndrome de Resposta Inflamatória Sistêmica/patologiaRESUMO
A single case of a subdiaphragmatic bronchopulmonary sequestrated cyst removed at laparotomy is reported. Needle aspiration of the cyst prior to removal revealed a high amylase although the serum amylase was normal. A review of the literature failed to reveal a similar case. The ultrasound, computed tomography and histopathology of this case are described.