RESUMO
Metalloid tellurium is characterized as a chemical element belonging to the chalcogen group without known biological function. However, its compounds, especially the oxyanions, exert numerous negative effects on both prokaryotic and eukaryotic organisms. Recent evidence suggests that increasing environmental pollution with tellurium has a causal link to autoimmune, neurodegenerative and oncological diseases. In this review, we provide an overview about the current knowledge on the mechanisms of tellurium compounds' toxicity in bacteria and humans and we summarise the various ways organisms cope and detoxify these compounds. Over the last decades, several gene clusters conferring resistance to tellurium compounds have been identified in a variety of bacterial species and strains. These genetic determinants exhibit great genetic and functional diversity. Besides the existence of specific resistance mechanisms, tellurium and its toxic compounds interact with molecular systems, mediating general detoxification and mitigation of oxidative stress. We also discuss the similarity of tellurium and selenium biochemistry and the impact of their compounds on humans.
Assuntos
Células Eucarióticas/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Células Procarióticas/efeitos dos fármacos , Telúrio/efeitos adversos , Ânions/efeitos adversos , Bactérias/efeitos dos fármacos , Poluição Ambiental/análise , Humanos , Selênio/química , Telúrio/química , Telúrio/toxicidadeRESUMO
Quantum dots (QDs) are a class of engineered nanoparticles (ENPs) with several biomedical, industrial and commercial applications. However, their metabolism and detoxification process in aquatic invertebrates and environmental health hazards remain unclear. This study investigate the transcriptional changes of metallothioneins (MTs) isoforms (mt10IIIa and mt20IV) induced by CdTe QDs, in comparison with its dissolved counterpart, in the marine mussel Mytilus galloprovincialis. Mussels were exposed to CdTe QDs and to the same Cd concentration (10 µg Cd L-1) of dissolved Cd for 14 days and mt transcription levels were measured by real time quantitative PCR (qPCR). Tissue specific mt transcription patterns were observed in mussels exposed to both Cd forms, wherein the gills were a more sensitive organ compared to the digestive gland. No significant changes were observed in mt10IIIa transcription levels in mussels exposed to both Cd forms. In contrast, transcription of mt20IV was tissue and exposure time dependent, with higher mt20IV mRNA levels in mussels exposed to QDs and dissolved Cd when compared to unexposed mussels. Multivariate analysis indicates particle-specific effects after 14 days of exposure and a dual role of MTs in the QD metabolism and in the protection against oxidative stress in mussels exposed to Cd-based ENPs.
Assuntos
Compostos de Cádmio/efeitos adversos , Metalotioneína/genética , Mytilus/efeitos dos fármacos , Pontos Quânticos/efeitos adversos , Telúrio/efeitos adversos , Transcrição Gênica/efeitos dos fármacos , Poluentes Químicos da Água/efeitos adversos , Animais , Brânquias/efeitos dos fármacos , Brânquias/metabolismo , Metalotioneína/metabolismo , Estresse Oxidativo , Isoformas de Proteínas/genética , Isoformas de Proteínas/metabolismoRESUMO
Tellurium (Te) is a semimetal rare element in nature. Together with oxygen, sulfur (S), and selenium (Se), Te is considered a member of chalcogen group. Over recent decades, Te applications continued to emerge in different fields including metallurgy, glass industry, electronics, and applied chemical industries. Along these lines, Te has recently attracted research attention in various fields. Though Te exists in biologic organisms such as microbes, yeast, and human body, its importance and role and some of its potential implications have long been ignored. Some promising applications of Te using its inorganic and organic derivatives including novel Te nanostructures are being introduced. Before discovery and straightforward availability of antibiotics, Te had considered and had been used as an antibacterial element. Antilishmaniasis, antiinflammatory, antiatherosclerotic, and immuno-modulating properties of Te have been described for many years, while the innovative applications of Te have started to emerge along with nanotechnological advances over the recent years. Te quantum dots (QDs) and related nanostructures have proposed novel applications in the biological detection systems such as biosensors. In addition, Te nanostructures are used in labeling, imaging, and targeted drug delivery systems and are tested for antibacterial or antifungal properties. In addition, Te nanoparticles show novel lipid-lowering, antioxidant, and free radical scavenging properties. This review presents an overview on the novel forms of Te, their potential applications, as well as related toxicity profiles.
Assuntos
Antioxidantes/uso terapêutico , Biologia/história , Descoberta de Drogas/história , Drogas em Investigação/uso terapêutico , Nanoestruturas/uso terapêutico , Telúrio/uso terapêutico , Animais , Anti-Infecciosos/efeitos adversos , Anti-Infecciosos/química , Anti-Infecciosos/uso terapêutico , Anti-Hipertensivos/efeitos adversos , Anti-Hipertensivos/química , Anti-Hipertensivos/uso terapêutico , Antioxidantes/efeitos adversos , Antioxidantes/química , Descoberta de Drogas/tendências , Drogas em Investigação/efeitos adversos , Drogas em Investigação/química , Sequestradores de Radicais Livres/efeitos adversos , Sequestradores de Radicais Livres/química , Sequestradores de Radicais Livres/uso terapêutico , História do Século XIX , História do Século XX , História do Século XXI , Humanos , Fatores Imunológicos/efeitos adversos , Fatores Imunológicos/química , Fatores Imunológicos/uso terapêutico , Microbiologia/história , Nanoestruturas/efeitos adversos , Nanoestruturas/química , Nanotecnologia/história , Nanotecnologia/tendências , Pontos Quânticos/efeitos adversos , Pontos Quânticos/química , Pontos Quânticos/uso terapêutico , Telúrio/efeitos adversos , Telúrio/químicaRESUMO
Thorough characterization of toxic effects of nanoparticles (NP) is desirable due to the increasing risk of potential environmental contamination by NP. In the current study, we combined three recently developed proteomics approaches to assess the effects of Au, CuO, and CdTe NP on the innate immune system. The human monocyte cell line THP-1 was employed as a model. The anticancer drugs camptothecin and doxorubicin were used as positive controls for cell death, and lipopolysaccharide was chosen as a positive control for proinflammatory activation. Despite equivalent overall toxicity effect (50 ± 10% dead cells), the three NP induced distinctly different proteomics signatures, with the strongest effect being induced by CdTe NP, followed by CuO and gold NP. The CdTe toxicity mechanism involves down-regulation of topoisomerases. The effect of CuO NP is most reminiscent of oxidative stress and involves up-regulation of proteins involved in heat response. The gold NP induced up-regulation of the inflammatory mediator, NF-κB, and its inhibitor TIPE2 was identified as a direct target of gold NP. Furthermore, gold NP triggered activation of NF-κB as evidenced by phosphorylation of the p65 subunit. Overall, the combined proteomics approach described here can be used to characterize the effects of NP on immune cells.
Assuntos
Imunidade Inata/efeitos dos fármacos , Inflamação/genética , Nanopartículas Metálicas/efeitos adversos , Proteoma/genética , Proteômica , Compostos de Cádmio/efeitos adversos , Camptotecina/administração & dosagem , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Cobre/efeitos adversos , Citotoxinas/efeitos adversos , Doxorrubicina/administração & dosagem , Ouro/efeitos adversos , Humanos , Imunidade Inata/genética , Inflamação/induzido quimicamente , Lipopolissacarídeos/administração & dosagem , Monócitos/efeitos dos fármacos , Monócitos/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Proteoma/efeitos dos fármacos , Telúrio/efeitos adversosRESUMO
The wide-ranging applications of fluorescent semiconductor quantum dots (QDs) have triggered increasing concerns about their biosafety. Most QD-related toxicity studies focus on the subcellular processes in cultured cells or global physiological effects on whole animals. However, it is unclear how QDs affect subcellular processes in living organisms, or how the subcellular disturbance contributes to the overall toxicity. Here the behavior and toxicity of QDs of three different sizes in Caenorhabditis elegans (C. elegans) are systematically investigated at both the systemic and the subcellular level. Specifically, clear size-dependent distribution and toxicity of the QDs in the digestive tract are observed. Short-term exposure of QDs leads to acute toxicity on C. elegans, yet incurring no lasting, irreversible damage. In contrast, chronic exposure of QDs severely inhibits development and shortens lifespan. Subcellular analysis reveals that endocytosis and nutrition storage are disrupted by QDs, which likely accounts for the severe deterioration in growth and longevity. This work reveals that QDs invasion disrupts key subcellular processes in living organisms, and may cause permanent damage to the tissues and organs over long-term retention. The findings provide invaluable information for safety evaluations of QD-based applications and offer new opportunities for design of novel nontoxic nanoprobes.
Assuntos
Caenorhabditis elegans/efeitos dos fármacos , Pontos Quânticos/efeitos adversos , Animais , Compostos de Cádmio/efeitos adversos , Compostos de Cádmio/química , Semicondutores/efeitos adversos , Telúrio/efeitos adversos , Telúrio/químicaRESUMO
As quantum dots (QDs) are widely used in biomedical applications, the number of studies focusing on their biological properties is increasing. While several studies have attempted to evaluate the toxicity of QDs towards neural cells, the in vivo toxic effects on the nervous system and the molecular mechanisms are unclear. The aim of the present study was to investigate the neurotoxic effects and the underlying mechanisms of water-soluble cadmium telluride (CdTe) QDs capped with 3-mercaptopropionic acid (MPA) in Caenorhabditis elegans (C. elegans). Our results showed that exposure to MPA-capped CdTe QDs induced behavioral defects, including alterations to body bending, head thrashing, pharyngeal pumping and defecation intervals, as well as impaired learning and memory behavior plasticity, based on chemotaxis or thermotaxis, in a dose-, time- and size-dependent manner. Further investigations suggested that MPA-capped CdTe QDs exposure inhibited the transporters and receptors of glutamate, serotonin and dopamine in C. elegans at the genetic level within 24 h, while opposite results were observed after 72 h. Additionally, excessive reactive oxygen species (ROS) generation was observed in the CdTe QD-treated worms, which confirmed the common nanotoxicity mechanism of oxidative stress damage, and might overcome the increased gene expression of neurotransmitter transporters and receptors in C. elegans induced by long-term QD exposure, resulting in more severe behavioral impairments.
Assuntos
Cádmio , Proteínas de Caenorhabditis elegans/metabolismo , Caenorhabditis elegans/metabolismo , Proteínas de Transporte/metabolismo , Dopamina/metabolismo , Ácido Glutâmico/metabolismo , Síndromes Neurotóxicas/metabolismo , Pontos Quânticos , Espécies Reativas de Oxigênio/metabolismo , Serotonina/metabolismo , Telúrio , Animais , Cádmio/efeitos adversos , Cádmio/química , Cádmio/farmacologia , Pontos Quânticos/efeitos adversos , Pontos Quânticos/química , Telúrio/efeitos adversos , Telúrio/química , Telúrio/farmacologiaRESUMO
To impart biocompatibility, stability, and specificity to quantum dots (QDs)-and to reduce their toxicity-it is essential to carry out surface modification. However, most surface-modification processes are costly, complicated, and time-consuming. In addition, the modified QDs often have a large size, which leads to easy aggregation in biological environments, making it difficult to excrete them from in vivo systems. To solve these problems, three kinds of conventional polymers, namely, polyvinyl alcohol (PVA, neutral), sodium polystyrene sulfonate (PSS, negative charged), and poly(diallyl dimethyl ammonium chloride) (PDDA, positive charged) were selected to modify the surface of QDs at low cost via a simple process in which the size of the QDs was kept small after modification. The effect of polymer modification on the photoluminescence (PL) properties of the QDs was systematically investigated. High quantum yields (QYs) of 65 % were reached, which is important for the realization of bio-imaging. Then, the cytotoxicity of CdTe QD-polymer composites was systematically investigated via MTT assay using the Cal27 and HeLa cell lines, especially for high concentrations of QD-polymer composites in vitro. The experimental results showed that the cytotoxicity decreased in the order CdTe-PDDA>CdTe>CdTe-PSS>CdTe-PVA, indicating that PSS and PVA can reduce the toxicity of the QDs. An obvious cytotoxicity of CdTe-PVA and CdTe-PSS was present until 120â h for the Cal27 cell line and until 168â h for the HeLa cell line. At last, the Cal27 cell line was selected to realize bio-imaging using CdTe-PSS and CdTe-PVA composites with different emission colors under one excitation wavelength.
Assuntos
Compostos de Cádmio/química , Luminescência , Imagem Molecular , Polímeros/química , Polímeros/economia , Pontos Quânticos/química , Telúrio/química , Compostos de Cádmio/efeitos adversos , Compostos de Cádmio/economia , Linhagem Celular Tumoral , Células HeLa , Humanos , Estrutura Molecular , Tamanho da Partícula , Processos Fotoquímicos , Pontos Quânticos/efeitos adversos , Pontos Quânticos/economia , Propriedades de Superfície , Telúrio/efeitos adversos , Telúrio/economiaRESUMO
Tellurium has long appeared as a nearly 'forgotten' element in Biology, with most studies focusing on tellurite, tellurate and a handful of organic tellurides. During the last decade, several discoveries have fuelled a renewed interest in this element. Bioincorporation of telluromethionine provides a new approach to add heavy atoms to selected sites in proteins. Cadmium telluride (CdTe) nanoparticles are fluorescent and may be used as quantum dots in imaging and diagnosis. The antibiotic properties of tellurite, long known yet almost forgotten, have attracted renewed interest, especially since the biochemical mechanisms of tellurium cytotoxicity are beginning to emerge. The close chemical relationship between tellurium and sulfur also transcends into in vitro and in vivo situations and provides new impetus for the development of enzyme inhibitors and redox modulators, some of which may be of interest in the field of antibiotics and anticancer drug design.
Assuntos
Telúrio/química , Telúrio/metabolismo , Animais , Antibacterianos/química , Bactérias/metabolismo , Catálise , Fungos/metabolismo , Humanos , Estrutura Molecular , Compostos Orgânicos/química , Oxirredução , Plantas/metabolismo , Telúrio/efeitos adversosRESUMO
The tellurium oxyanion tellurite is toxic for most organisms and it seems to alter a number of intracellular targets. In this work the toxic effects of tellurite upon Escherichia coli [4Fe-4S] cluster-containing dehydratases was studied. Reactive oxygen species (ROS)-sensitive fumarase A (FumA) and aconitase B (AcnB) as well as ROS-resistant fumarase C (FumC) and aconitase A (AcnA) were assayed in cell-free extracts from tellurite-exposed cells in both the presence and absence of oxygen. While over 90 % of FumA and AcnB activities were lost in the presence of oxygen, no enzyme inactivation was observed in anaerobiosis. This result was not dependent upon protein biosynthesis, as determined using translation-arrested cells. Enzyme activity of purified FumA and AcnB was inhibited when exposed to an in vitro superoxide-generating, tellurite-reducing system (ITRS). No inhibitory effect was observed when tellurite was omitted from the ITRS. In vivo and in vitro reconstitution experiments with tellurite-damaged FumA and AcnB suggested that tellurite effects involve [Fe-S] cluster disabling. In fact, after exposing FumA to ITRS, released ferrous ion from the enzyme was demonstrated by spectroscopic analysis using the specific Fe(2+) chelator 2,2'-bipyridyl. Subsequent spectroscopic paramagnetic resonance analysis of FumA exposed to ITRS showed the characteristic signal of an oxidatively inactivated [3Fe-4S](+) cluster. These results suggest that tellurite inactivates enzymes of this kind via a superoxide-dependent disabling of their [4Fe-4S] catalytic clusters.
Assuntos
Escherichia coli , Hidroliases/antagonistas & inibidores , Proteínas Ferro-Enxofre/antagonistas & inibidores , Telúrio/efeitos adversos , Aconitato Hidratase/antagonistas & inibidores , Aerobiose , Anaerobiose , Escherichia coli/efeitos dos fármacos , Escherichia coli/metabolismo , Compostos Ferrosos/química , Compostos Ferrosos/metabolismo , Fumarato Hidratase/antagonistas & inibidores , Hidroliases/química , Oxirredutases Intramoleculares/antagonistas & inibidores , Oxirredutases Intramoleculares/química , Proteínas Ferro-Enxofre/química , Análise Espectral , Superóxidos/metabolismoAssuntos
Centrais Elétricas , Liberação Nociva de Radioativos , Neoplasias da Glândula Tireoide/etiologia , Criança , Feminino , Humanos , Radioisótopos do Iodo/efeitos adversos , Masculino , Programas de Rastreamento , Prevalência , Radioisótopos/efeitos adversos , República de Belarus , Telúrio/efeitos adversos , Neoplasias da Glândula Tireoide/epidemiologia , Neoplasias da Glândula Tireoide/prevenção & controle , UcrâniaRESUMO
Neuropathy in animals is either genetically determined or is provoked by chemical compounds or physical injury. Diabetes in mice and rats may be spontaneous or induced, but a true copy of diabetic neuropathy in man is not yet available. Painful neuropathy occurs after nerve constriction or neuroma formation. A mouse mutant with delayed Wallerian degeneration demonstrates the pivotal role of this process for the regeneration of injured axons. Surprisingly, the neurotoxic effect of cisplatin which is severe in cancer patients has not yet unambiguously been reproduced in animals. Genetically determined diseases in mutants or transgenic animals may affect the myelination of peripheral axons. 'Trembler mice' are deficient in myelin and possibly correspond to CMT IA in man. The relation of sensory neuronopathies in mice, rats and dogs to human diseases is not yet clear. Motor neuronopathies in experimental animals have attracted much interest, because the recent discovery of motoneuronotrophic factors has raised high hopes. Most of the mutants described have not been appropriately studied, and the mouse mutant 'motoneurone disease' (mnd) eventually was found to have Batten's disease. None of the few more thoroughly studied models is probably a copy of human disease, although they may none the less help to test new therapies.
Assuntos
Neuropatias Diabéticas/induzido quimicamente , Animais , Antineoplásicos/efeitos adversos , Cisplatino/efeitos adversos , Neuropatias Diabéticas/fisiopatologia , Cães , Neuropatias Hereditárias Sensoriais e Autônomas , Humanos , Masculino , Camundongos , Degeneração Neural , Regeneração Nervosa , Nervos Periféricos/fisiopatologia , Radioisótopos/efeitos adversos , Ratos , Telúrio/efeitos adversosRESUMO
Occupational and environmental medicine affords encounters with many unusual toxins, ranging from exotic metals to rocket fuels. Twelve of the most unusual industrial toxins are reviewed here and their clinical manifestations and treatments explored: acetonitrile, acrylonitrile, boron hydrides, dimethylaminopropionitrile, dimethylformamide, hydrazines, methyl isocyanate, 2-nitropropane, phosphine, Stalinon, tellurium, and vanadium.
Assuntos
Isocianatos , Doenças Profissionais/induzido quimicamente , Acetonitrilas/efeitos adversos , Acrilonitrila/efeitos adversos , Aminopropionitrilo/efeitos adversos , Aminopropionitrilo/análogos & derivados , Cianatos/efeitos adversos , Humanos , Hidrazinas/efeitos adversos , Nitroparafinas/efeitos adversos , Exposição Ocupacional/efeitos adversos , Fosfinas/efeitos adversos , Propano/efeitos adversos , Propano/análogos & derivados , Telúrio/efeitos adversos , Vanádio/efeitos adversosAssuntos
Cárie Dentária/etiologia , Fenômenos Fisiológicos da Nutrição/efeitos dos fármacos , Telúrio/farmacologia , Animais , Ácido Ascórbico/farmacologia , Cisteína/farmacologia , Cárie Dentária/metabolismo , Ratos , Ratos Endogâmicos , Telúrio/administração & dosagem , Telúrio/efeitos adversos , Ácido Tióctico/farmacologiaRESUMO
In 3 experiments, 684 newly hatched White Pekin ducklings were fed (for 15 to 28 days) a commercial starter mash that was adequate in selenium and vitamin E (Se-E) content, either alone or with supplements of Ag (3,000 mg/kg of feed, as acetate), Cu (1,500 mg/kg, as sulfate), Co (200 or 500 mg/kg, as chloride), Te (500 mg/kg, as tetrachloride), Cd (100 or 500 mg/kg, as sulfate), Zn (3,000 or 6,000 mg/kg, as sulfate), or V (100 mg/kg, as vanadate). The ducklings fed Ag, Cu, Co, Te, Cd, and Zn frequently developed lesions characteristic of Se-E deficiency, such as necrosis of skeletal and cardiac muscle and of smooth muscle of the gizzard and intestine. Complete protection from the muscle lesions produced by Cu, Co, Te, Cd, and Zn supplements was provided by vitamin E (200 IU of alpha-tocopherol acetate/kg) and Se (2 mg/kg, as selenite). Ducklings fed Ag were protected by supplements of vitamin E and partial protection was achieved by Se addition. The birds fed excessive Zn developed pancreatic necrosis and fibrosis that was not prevented by supplements of Se or vitamin E. Terminally, blood glutathione peroxidase activity was low and hepatic Se concentration was increased in the ducklings fed Ag. However, neither blood glutathione peroxidase activity nor hepatic Se concentrations was consistently abnormal in ducklings fed other trace elements, although lesions of Se-E deficiency were often present in these animals.
Assuntos
Patos , Doenças das Aves Domésticas/induzido quimicamente , Selênio/uso terapêutico , Oligoelementos/efeitos adversos , Deficiência de Vitamina E/veterinária , Vitamina E/uso terapêutico , Animais , Cádmio/efeitos adversos , Cobalto/efeitos adversos , Cobre/efeitos adversos , Dieta , Masculino , Músculos/patologia , Doenças das Aves Domésticas/patologia , Doenças das Aves Domésticas/prevenção & controle , Prata/efeitos adversos , Telúrio/efeitos adversos , Deficiência de Vitamina E/induzido quimicamente , Deficiência de Vitamina E/prevenção & controle , Zinco/efeitos adversosRESUMO
Forty-two weanling pigs were allotted to 7 groups and fed (for 10 weeks) a commercial ration that was adequate in selenium and vitamin E (Se-E) content, either alone or with supplements of Ag (3,000 mg/kg of feed, as acetate), Co (500 mg/kg, as chloride), Te (500 mg/kg, as tetrachloride), Zn (3,000 mg/kg, as sulfate), Cd (500 mg/kg, as sulfate), or V (200 mg/kg, as vanadate). The pigs fed the Ag supplement died after 25 to 39 days and had lesions characteristic of Se-E deficiency with accumulations of serous transudates in body cavities and hepatic and cardiac necrosis. In the pigs fed the Ag supplement, there was high hepatic Se content terminally; blood glutathione peroxidase (GSH-Px) activity decreased to low levels several weeks before the pigs died with lesions of Se-E deficiency. Macroscopic lesions of Se-E deficiency were not found in pigs fed Co, Te, Zn, Cd, or V. However, evidence of Se-E deficiency, as indicated by microscopically detected necrosis of cardiac and skeletal muscle, was present in 50% to 65% of the pigs fed Co or Te and occasionally in pigs fed Zn, Cd, and V supplements. The pigs fed Te had marked decrease of blood GSH-Px activity over the last 6 weeks of the feeding period. No consistently abnormal values for blood GSH-Px activity or terminal hepatic Se content were observed in pigs fed Co, Zn, Cd, or V. The pigs fed the Zn supplement grew as rapidly as the control pigs. Evidence of V toxicosis was observed as severe growth suppression, mortality, and marked enteritis and cystitis (with accompanying hydroureter in 1 pig).
Assuntos
Metais/efeitos adversos , Selênio/deficiência , Doenças dos Suínos/etiologia , Deficiência de Vitamina E/veterinária , Animais , Cádmio/efeitos adversos , Cobalto/efeitos adversos , Fígado/patologia , Masculino , Músculos/patologia , Miocárdio/patologia , Prata/efeitos adversos , Suínos , Doenças dos Suínos/patologia , Telúrio/efeitos adversos , Vanádio/efeitos adversos , Deficiência de Vitamina E/etiologia , Deficiência de Vitamina E/patologia , Zinco/efeitos adversosRESUMO
Single endotracheal injections of tellurium and tellurium dioxide, at dosage levels sufficient to cause observable stress in rats, did not result in a progressive fibrotic tissue response after 180 days. The observation period of 180 days is insufficient to assess the lack of tumorigenic potential of these compounds and, therefore, no conclusions on this point are to be inferred.