Effects of Arctium lappa aqueous extract on lipid profile and hepatic enzyme levels of sucrose-induced metabolic syndrome in female rats

ABSTRACT Arctium lappa is known to have antioxidant and antidiabetic effects in traditional medicine. Objectives: The aim of this paper was to study the effects of A. lappa root extract (AE) on lipid profile and hepatic enzyme levels in sucrose-induced metabolic syndrome (MS) in female rats. The study used 40 adult female Wistar rats weighing 150 g-250 g randomly divided into five groups: control, metabolic syndrome (MS), metabolic syndrome+AE at 50,100, 200 mg/kg. MS was induced by administering 50% sucrose in drinking water for 6 weeks. AE was intra-peritoneally administered daily at doses of 50,100, and 200 mg/kg for two sequential weeks at the end of the fourth week in metabolic syndrome rats. Twenty-four hours after the last administration of AE, blood was collected and centrifuged, and then the serum was used for the measurement of lipid profile and hepatic enzyme. Serum glucose, insulin, fasting insulin resistance index, body weight, water intake, lipid profile, and hepatic enzymes were significantly increased although food intake was decreased in MS rats compared to the control rats. The lipids and liver enzymes were reduced by AE extracts in the MS group. This study showed that the A. lappa root aqueous extract exhibits a hypolipidemic activity of hyperlipidemic rats. This activity is practically that of a triple-impact antioxidant, hypolipidemic, and hepatoprotective.

Liver transplantation organ allocation between Child and MELD.

Liver transplantation (LT) has been established as the most effective therapy for acute and chronic liver diseases over the last few decades due to its excellent long-term results. At the beginning of the LT era, donor organs were allocated based on waiting time. However, as the number of LT candidates consistently increased, a specific allocation system became necessary to prioritize the large number of patients waiting for a limited pool of organs. The LT candidates were categorized into different urgency levels based on their hospital status, degree of liver disease as measured by the Child-Turcotte-Pugh score, and accompanying complications of liver disease, such as ascites, variceal bleeding or hepatocellular carcinoma. The majority of European countries, including Austria, still rely on this organ allocation system. In the United States, however, a new allocation system based on the risk of death without transplantation, assessed by the Model for End-stage Liver Disease (MELD), was initiated in February 2002. Recent reports have shown that the introduction of the MELD system led to a reduction in waiting list mortality, but also that the MELD score has several limitations that call for further refinements. In the transplant community there are reasonable doubts that MELD is actually superior to the Child-Turcotte Pugh score. Therefore, the optimal liver organ allocation system is yet to be defined.

Preoperative assessment of liver function.

At the present time, the decision to resect and the choice of the extent ofa hepatic resection are largely based on surgical judgment. The CP score is the best assessment tool we can now employ. There is uniform agreement that even segmental resections are not possible in the vast majority of Child Class B patients, CP score 7 to 9. The CP score can be augmented by radiographic testing, ICG retention testing, and by assessing tumor extent and the severity of the patient's cirrhosis at surgery. Surgeons need a simple means to assist with liver function evaluation--a test to augment the CP score. Although determining ICG retention is simple, it is questionable whether it adds to one's ability to define the poor-risk patient with better accuracy than the CP score. Abundant data exist to dispute the accuracy and reproducibility of ICG retention. That surgeons use it says more about the fervent desire to find a test that supports clinical judgment in these difficult patients than the scientific validity of the test. Whether a series of tests would better define the Child-Pugh Class A patient who is also a relatively poor risk is not clear at present. Many investigations demonstrate the correlation of various assessment tools with each other, yet nothing distinguishes them in predicting risk beyond what is learned from the CP score. In a group of CP Class A patients, the extent of the disease, the nature of underlying cirrhosis, and the extent of resection provide the clinical backdrop against which a decision for resection must be made. It may well be that one test may not do it, or that one single assessment of the ICG or the 15-minute receptor volume of GSA may be inadequate to project the nuances of liver function. Thus, 99m-Tc GSA scintigraphy will provide volumetric receptor data, as well as kinetic distribution curves, and may prove a useful test in the future. Whether GSA is ultimately to be proven useful requires a correlation of the test with actual clinical outcomes, rather than correlation with other tests or with the CP score. Discovering which patients are the poor risk Child Class A patients is the desired goal. To have value, the GSA scan must augment, not mimic, the CP score. In view of the fact that experienced surgeons appear to be astute in their ability to select patients for hepatic resection, finding a more refined test will require large numbers of patients at several centers to correlate the test results and the outcomes against the spectrum of postoperative liver failure, including death. It appears that one lesson learned from portal vein embolization is that functional liver volume can be preserved. The compensatory hyperplasia that occurs in the contralateral hepatic lobe demonstrates two important features: (1) function of the opposite lobe has been transferred when evaluated by 99m-Tc-GSA, and (2) one considerable metabolic drain on the postoperative recovery from hepatic resection (ie, liver regeneration) can be attended to before the surgery. Cirrhotic livers do regenerate, but more slowly. Thus, pregrowing the remnant section of liver eliminates one stress on liver reserves following liver resection. For hepatocellular carcinoma or metastasis in cirrhotic patients, portal vein occlusion may be the best way to improve hepatic functional reserve. ICG retention may not corroborate return-to-baseline hepatic function within 2 weeks of portal vein occlusion,but may demonstrate a return to baseline when studied 6 to 8 weeks following the procedure. 99m-Tc-GSA is presently the best means to document compensatory hyperplasia and, possibly, a shift of functional reserve to the planned remnant of a more than four-segment hepatic resection. Whether this will predict the safe outcome of resection remains to be seen.

Liver recipients undergoing hepatic transplantation and subsequent retransplantation: a comparison between clinical characteristics.

BACKGROUND/AIMS: In order to establish a rational strategy for organ distribution and optimal patient management, we postulate it is mandatory not only to understand the pathophysiology of failing grafts but also to better recognize the baseline clinical characteristics of the recipients shortly before receiving a second liver allograft. METHODOLOGY: Between March 1986 and December 1997, 1061 patients underwent 1087 orthotopic liver transplantation at three Hospitals in Madrid (122 retransplants). RESULTS: Mean follow-up was 36 months (range, 1-90), 40.6% of the recipients were alive and survival at 1, 3 and 5 years was 62%, 53%, and 46%. Almost 50% of the recipients were UNOS 1 before retransplantation. In comparison to p-OLT (15% UNOS 1), it is clearly shown that the retransplants have been performed in the sickest patients with more adverse prognostic indicators (higher AST, bilirubin, creatinine serum levels, higher Child-Pugh score, higher rate of ascites and lower serum levels of albumin and prothrombin activity). CONCLUSIONS: Liver retransplantation is acceptable and significant differences in recipients' baseline characteristics suggest an impact on lower survival.

Clinical features of patients with HCC who are negative for both HBV and HCV markers.

BACKGROUND/AIMS: The aim of this study is to clarify the clinical features of hepatocellular carcinoma that are negative for both hepatitis B surface antigen and anti-hepatitis C antibody. METHODOLOGY: Patients were classified according to viral markers: 45 patients (82%) had hepatitis B (B-HCC), 467 patients (82%) had hepatitis C (C-HCC), and 53 patients (9%) had neither hepatitis B nor hepatitis C (NBNC-HCC). Differences in clinical parameters among these three groups were analyzed. RESULTS: Patients with NBNC-HCC were older than B-HCC and C-HCC patients. The incidence of alcoholism in NBNC-HCC patients was higher than in C-HCC patients. Patients with NBNC-HCC had similar rates of positive antibody to hepatitis B core antigen as did patients with C-HCC. NBNC-HCC patients were further classified according to median age. The younger group showed a greater tendency towards alcoholism than did the aged group. Liver functioning in the younger group was worse than in the older group. The older group had larger tumors than the younger group. CONCLUSIONS: The livers of younger NBNC-HCC patients were more cirrhotic, possibly because of alcoholism. Older NBNC-HCC patients presented with larger tumors, possibly because they did not receive regular medical check-ups due to their relatively preserved liver function.

Colestasis intrahepática del embarazo, un riesgo fetal impredecible: presentación de un caso y revisión de la literatura / Intrahepatic Cholestasis of pregnancy, an umpredictable fetal risk: a case report and liteature review

Se presenta un caso de colestasis intrahepática del embarazo (CIE) que tuvo muerte fetal unas horas después de un registro cardiotocográfico normal. Es una mujer de 35 años con el antecedente de dos embarazos que cursaron con dicha enfermedad y tuvieron un buen desenlace. En el último embarazo tuvo nuevamente un cuadro clínico y bioquímico característicos de CIE. La evolución cardiotocográfica y ecográfica del embarazo fueron normales, salvo por tratarse de un feto pequeño para la edad de crecimiento armónico. A las 35 4/7 semanas inició actividad uterina prodrómica y una prueba de registro basal fue reactiva. Ocho horas después la paciente regresó para revalorar su hospitalización y se demostró la existencia de un óbito fetal. El estudio genético del producto y la valoración histológica de la placenta, fueron normales. La colestasis materna revirtió unas semanas después del parto.

Abnormal findings on liver function tests. Interpreting results to narrow the diagnosis and establish a prognosis.

Evaluating abnormal liver test results requires careful attention to the corresponding clinical data obtained during history taking and physical examination. Generally, it is helpful to separate liver tests into three categories: tests that assess synthetic function, tests that assess hepatocellular necrosis (hepatocellular enzymes), and tests that assess cholestasis. The clinical setting together with the specific pattern of liver function abnormalities can narrow differential diagnosis and provide a cost-effective approach to assessing patients and identifying those who need liver biopsy.

Efeitos do implante subdérmico de acetato de nomegestrol sobre o metabolismo de carboidatos, lipoproteínas séricas e funçäo hepática / The effects of nomegestrol acetate subdermal implant on carboydrate metabolism. serum lipoproteins and on hepatic function

Objetivos: avaliar variações de peso corporal, pressao arterial, glicemia em jejum, HbA1C, insulina, coleterol total, HDL-C, LDL-C, triglicérides, TGO, TGP, GGT e bilirrubina em mulheres usuárias de um implate único, subdérmico, de Silástico, contendo 55 mg (+10 por cento) de acetato de nomegestrol, durante dois anos. Métodos: dezoito voluntárias saudáveis e em idade reprodutiva, que desejavam fazer uso de anticoncepcionais e nao apresentavam contra-indicaçoes para o uso de contracepçao hormonal, participaram deste estudo. Todas as mulheres foram avaliadas antes do início do tratamento e a seguir, acompanhadas por um período de dois anos. Ao final do primeiro ano, as cápsulas foram retiradas e novas cápsulas foram inseridas. Resultados: o peso corporal aumentou de 54,9 + 1,5 kg na admissao para 55,3 + 2,0 Kg no 12º mês de uso (p<0,05), e para 56,0 + 2,7 Kg no 24º mês de uso. Registrou-se discreto aumento da pressao arterial, tanto sistólica quanto diastólica, no mês 12 (p<0,01). No mês 24, a pressao arterial nao era significativamente diferente dos valores de admissao. Todos os valores estiveram dentro dos limites da normalidade. Insulina, HbA1C, LDL-C e GGT permaneceram inalterados durante os vinte e quatro meses de uso do implante. Diminuiçao significativa do colesterol total (p<0,05) foi observada no 3º mês e de HDL-C (p<0,01) no 6º mês. Observou-se aumento significativo de triglicérides (p<0,05) apenas no 12º mês. Todas as alteraçoes de lipoproteínas foram inconsistentes, e os valores estiveram dentro dos limites da normalidade. Aumentos significativos dos níveis de glicemia em jejum (p<0,05 e p<0,01) foram observados respectivamente no 3º e no 6º mês. Diminuiçoes significativas da TGO (p<0,05, p<0,01 e p<0,05) foram observadas respectivamente no 6º, 18º e 24º mês e da TGP (p<0,05) no 18º mês. Somente se observou aumento significativo de bilirrubina (p<0,05) no 3º mês de uso do implante. Todas estas variaçoes permaneceram dentro dos limites da normalidade. Conclusoes: esses resultados demonstraram que, dentro dos limites da normalidade, as variaçoes de glicemia em jejum nao se correlacionaram às alteraçoes dos níveis de insulina. As alteraçoes discretas em lipoproteínas séricas, TGO, TGP e bilirrubina foram transitórias. Nao foram observados efeitos clínicos em lipoproteínas, metabolismo de carboidratos, níveis de insulina e funçao hepática entre as usuárias por dois anos.

[Hemodynamics, liver function and clinical follow-up after TIPS]. / Hämodynamik, Leberfunktion und klinischer Verlauf nach TIPS.

In 126 patients with liver cirrhosis treated electively with transjugular intrahepatic portosystemic stent shunt (TIPS) to prevent variceal rebleeding, the portosystemic pressure gradient decreased by 60%. In spite of this incomplete effect the risk for variceal rebleeding was still under 20% after 2 years. Only 1 patient died of variceal rebleeding. Shunt insufficiency occurred in 50%, mainly during the first year, but shunt function was restored in nearly all cases by radiologic intervention, i.e., redilatation or implantation of an additional stent. During the follow-up of 16 +/- 9 months, 21 patients (17%) died, one-third of them from progressive liver failure aggravated in 4 cases by severe drinking. De novo hepatic encephalopathy was observed in 10%, especially in older patients and patients with impaired liver function before TIPS. In such patients it is recommended that the shunt be dilated to 0.8 cm at most, and the TIPS procedure can be combined with transjugular embolization of the varices. The advantages of TIPS over both endoscopic sclerotherapy and drug treatment must be clarified in randomized studies, which have already been initiated in several centers.

[Liver function tests in a clinical comparison]. / Leberfunktionstests im klinischen Vergleich.

Various liver function tests were evaluated in regard to a quantitative estimation of the impairment of liver function related to the Child-Pugh classification in 32 patients with cirrhosis. Only the ICG-test revealed significant differences between healthy subjects and cirrhotic patients in stadium Child A, B and C. When ICG-dye retention values were plotted as a function of the individual score units of the Child-Pugh classification, a linear relationship with a correlation coefficient of 0.7 was obtained. In contrast to the ICG-test, the MEGX- and galactose elimination capacity (GEC)-test as well as static parameters of liver function (cholinesterase activity, prealbumin concentration, coagulation factor V and VII) resulted in less significant differentiation of the various Child classes. The MEGX-test, GEC, concentration of prealbumin, coagulation factor V and VII were only weakly correlated to the score units of the Child-Pugh index. The results of this study indicate that of all evaluated parameters only the ICG-test is suitable for objective and graduated analysis of liver function in patients with cirrhosis.