RESUMO
Baboons naturally infected with simian T lymphotropic virus (STLV) are a potentially useful model system for the study of vaccination against human T lymphotropic virus (HTLV). Here we expanded the number of available full-length baboon STLV-1 sequences from one to three and related the T cell responses that recognize the immunodominant Tax protein to the tax sequences present in two individual baboons. Continuously growing T cell lines were established from two baboons, animals 12141 and 12752. Next-generation sequencing (NGS) of complete STLV genome sequences from these T cell lines revealed them to be closely related but distinct from each other and from the baboon STLV-1 sequence in the NCBI sequence database. Overlapping peptides corresponding to each unique Tax sequence and to the reference baboon Tax sequence were used to analyze recognition by T cells from each baboon using intracellular cytokine staining (ICS). Individual baboons expressed more gamma interferon and tumor necrosis factor alpha in response to Tax peptides corresponding to their own STLV-1 sequence than in response to Tax peptides corresponding to the reference baboon STLV-1 sequence. Thus, our analyses revealed distinct but closely related STLV-1 genome sequences in two baboons, extremely low heterogeneity of STLV sequences within each baboon, no evidence for superinfection within each baboon, and a ready ability of T cells in each baboon to recognize circulating Tax sequences. While amino acid substitutions that result in escape from CD8+ T cell recognition were not observed, premature stop codons were observed in 7% and 56% of tax sequences from peripheral blood mononuclear cells from animals 12141 and 12752, respectively.IMPORTANCE It has been estimated that approximately 100,000 people suffer serious morbidity and 10,000 people die each year from the consequences associated with human T lymphotropic virus (HTLV) infection. There are no antiviral drugs and no preventive vaccine. A preventive vaccine would significantly impact the global burden associated with HTLV infections. Here we provide fundamental information on the simian T lymphotropic virus (STLV) naturally transmitted in a colony of captive baboons. The limited viral sequence heterogeneity in individual baboons, the identity of the viral gene product that is the major target of cellular immune responses, the persistence of viral amino acid sequences that are the major targets of cellular immune responses, and the emergence in vivo of truncated variants in the major target of cellular immune responses all parallel what are seen with HTLV infection of humans. These results justify the use of STLV-infected baboons as a model system for vaccine development efforts.
Assuntos
Produtos do Gene tax/química , Produtos do Gene tax/genética , Infecções por HTLV-I/virologia , Vírus Linfotrópico T Tipo 1 de Símios/isolamento & purificação , Linfócitos T/imunologia , Substituição de Aminoácidos , Animais , DNA Viral/genética , Produtos do Gene tax/imunologia , Genoma Viral , Infecções por HTLV-I/imunologia , Infecções por HTLV-I/transmissão , Sequenciamento de Nucleotídeos em Larga Escala , Imunidade Celular , Interferon gama/biossíntese , Interferon gama/imunologia , Papio anubis , Filogenia , Reação em Cadeia da Polimerase , Vírus Linfotrópico T Tipo 1 de Símios/imunologia , Linfócitos T/virologia , Fator de Necrose Tumoral alfa/biossíntese , Fator de Necrose Tumoral alfa/imunologiaRESUMO
Central African tropical forests are home to several species of non-human primates (NHPs), infected by Simian Immunodeficiency Virus (SIV). It is well-known that HIV-1 epidemic is due to cross-transmission and adaptation of SIV to humans. The main goal of this work was to investigate if a NHP bite is a risk factor for SIV acquisition. A cross-sectional study was performed in rural Cameroon on 246 bitten individuals (mostly by adult NHPs), matched, according to sex, age, and ethnicity (Bantus and Pygmies), with an equal number of not-bitten subjects. Following a serological assay for a wide range of SIVs, we observed a high level of indeterminate seroreactivity (25.8%) in the total population, whereas 68.9% were sero-negative and 5.3% HIV-1 positive. Bites do not appear to be a risk factor for SIV seroreactivity, in contrast to Simian Foamy Virus and Simian T-Lymphotropic Virus type 1 in the same studied population.
Assuntos
Mordeduras e Picadas/epidemiologia , Infecções por Retroviridae/epidemiologia , Infecções por Retroviridae/transmissão , Zoonoses/epidemiologia , Zoonoses/transmissão , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Animais , Camarões/epidemiologia , Cercopithecus , Criança , Pré-Escolar , Estudos Transversais , Feminino , Gorilla gorilla , Humanos , Masculino , Pessoa de Meia-Idade , Pan troglodytes , Infecções por Retroviridae/virologia , Fatores de Risco , Vírus da Imunodeficiência Símia/imunologia , Vírus Linfotrópico T Tipo 1 de Símios/imunologia , Vírus Espumoso dos Símios/imunologia , Inquéritos e Questionários , Adulto Jovem , Zoonoses/virologiaRESUMO
Human T-cell leukemia virus type 1 (HTLV-1) causes adult T-cell leukemia and inflammatory diseases. Because anti-HTLV-1 immune responses are critical for suppressing infected cells, enhancing cellular immunity is beneficial for the treatment of HTLV-1-associated diseases. Using simian T-cell leukemia virus type 1 (STLV-1) infected Japanese macaques, we analyzed the immune responses to viral antigens and the dynamics of virus-infected cells. The chemokine receptor CCR4 is expressed on STLV-1 infected cells, and administration of humanized monoclonal antibody to CCR4, mogamulizumab, dramatically decreased the number of STLV-1-infected cells in vivo. Concurrently, mogamulizumab treatment enhanced STLV-1 specific CD4(+) and CD8(+) T cell responses by simultaneously targeting CCR4(+) effector regulatory T (Treg) cells and infected cells. Mogamulizumab promoted the phagocytosis of CCR4(+) infected cells by macrophages, which likely enhanced antigen presentation. Vaccination with recombinant vaccinia virus (rVV) expressing viral antigens suppressed the proviral load and the number of Tax-expressing cells. Enhanced T-cell responses were also observed in some ATL patients who were treated with mogamulizumab. This study shows that mogamulizumab works not only by killing CCR4(+) infected cells directly, but also by enhancing T cell responses by increasing the phagocytosis of infected cells by antigen-presenting cells and suppressing CCR4(+) effector Treg cells.
Assuntos
Anticorpos Monoclonais Humanizados/administração & dosagem , Infecções por Deltaretrovirus/tratamento farmacológico , Vírus Linfotrópico T Tipo 1 Humano/efeitos dos fármacos , Receptores CCR4/metabolismo , Vírus Linfotrópico T Tipo 1 de Símios/efeitos dos fármacos , Animais , Anticorpos , Anticorpos Monoclonais Humanizados/farmacologia , Antígenos Virais/metabolismo , Linfócitos T CD4-Positivos/metabolismo , Linfócitos T CD8-Positivos/metabolismo , Infecções por Deltaretrovirus/virologia , Vírus Linfotrópico T Tipo 1 Humano/imunologia , Humanos , Macaca/imunologia , Macaca/virologia , Camundongos , Vírus Linfotrópico T Tipo 1 de Símios/imunologia , Linfócitos T Reguladores/metabolismo , Carga Viral/efeitos dos fármacosRESUMO
UNLABELLED: There are currently 5 million to 10 million human T-lymphotropic virus type 1 (HTLV-1)-infected people, and many of them will develop severe complications resulting from this infection. A vaccine is urgently needed in areas where HTLV-1 is endemic. Many vaccines are best tested in nonhuman primate animal models. As a first step in designing an effective HTLV-1 vaccine, we defined the CD8(+) and CD4(+) T cell response against simian T-lymphotropic virus type 1 (STLV-1), a virus closely related to HTLV-1, in olive baboons (Papio anubis). Consistent with persistent antigenic exposure, we observed that STLV-1-specific CD8(+) T cells displayed an effector memory phenotype and usually expressed CD107a, gamma interferon (IFN-γ), and tumor necrosis factor alpha (TNF-α). To assess the viral targets of the cellular immune response in STLV-1-infected animals, we used intracellular cytokine staining to detect responses against overlapping peptides covering the entire STLV-1 proteome. Our results show that, similarly to humans, the baboon CD8(+) T cell response narrowly targeted the Tax protein. Our findings suggest that the STLV-1-infected baboon model may recapitulate some of the important aspects of the human response against HTLV-1 and could be an important tool for the development of immune-based therapy and prophylaxis. IMPORTANCE: HTLV-1 infection can lead to many different and often fatal conditions. A vaccine deployed in areas of high prevalence might reduce the incidence of HTLV-1-induced disease. Unfortunately, there are very few animal models of HTLV-1 infection useful for testing vaccine approaches. Here we describe cellular immune responses in baboons against a closely related virus, STLV-1. We show for the first time that the immune response against STLV-1 in naturally infected baboons is largely directed against the Tax protein. Similar findings in humans and the sequence similarity between the human and baboon viruses suggest that the STLV-1-infected baboon model might be useful for developing a vaccine against HTLV-1.
Assuntos
Infecções por Deltaretrovirus/imunologia , Produtos do Gene tax/imunologia , Imunidade Celular , Vírus Linfotrópico T Tipo 1 de Símios/imunologia , Animais , Linfócitos T CD4-Positivos/imunologia , Linfócitos T CD8-Positivos/imunologia , Infecções por Deltaretrovirus/virologia , Modelos Animais de Doenças , Descoberta de Drogas , Humanos , Memória Imunológica , Interferon gama/genética , Papio , Proteoma , Fator de Necrose Tumoral alfa/genética , Carga Viral , Vacinas Virais/imunologiaRESUMO
BACKGROUND: Simian T-cell lymphoma/leukemia virus-1 (STLV-1) infection of non-human primates can serve as a model for human T-cell lymphoma/leukemia virus infection. METHODS: Two tantalus and 2 patas monkeys were transfused with intraspecies whole blood infected with STLV-1. Infection was determined by ELISA, western blot and DNA PCR analyses. The entire genome of the STLV-1 Tan 90 strain and some of the STVL-1 Pat74 strain were amplified using over-lapping primer-pairs and subsequently sequenced. RESULTS: Followup studies conducted over 2 years indicated that all 4 monkeys remained healthy despite being infected with STLV-1, as determined by PCR, cloning and sequencing analyses. ELISA and Western blot analyses indicated that both patas monkeys seroconverted within 2 months of transfusion, while one tantalus monkey required one year to seroconvert and the other never fully seroconverted. The tantalus monkey which never fully seroconverted, failed to react to HTLV-1 p24 Gag antigen. Sequence analyses indicated that, while unique, the deduced p24 Gag amino acid sequence of the STLV-1 Tan 90 strain used for infection was still highly homologous to the HTLV-1 p24 Gag amino acids present in the ELISA and WB assays. However, a mutation in the pol sequence of STLV-1 Tan 90 encoded a putative stop codon, while a common deletion in the pol/rex regulatory gene causes significant changes in the Pol, and p27 Rex proteins. These same mutations were also observed in the viral DNA of both recipient infected tantalus monkeys and were not present in the STLV-1 Pat 74 strain. CONCLUSION: Our data suggest that seroconversion to STLV-1 infection may be prolonged due to the above mutations, and that compensatory molecular events must have occurred to allow for virus transmission.
Assuntos
Infecções por Deltaretrovirus/veterinária , Genes pX/genética , Genes pX/imunologia , Mutação , RNA Polimerase Dependente de RNA/genética , RNA Polimerase Dependente de RNA/imunologia , Vírus Linfotrópico T Tipo 1 de Símios/imunologia , Sequência de Aminoácidos , Animais , Doenças Assintomáticas , Sequência de Bases , Western Blotting , Análise Mutacional de DNA , Infecções por Deltaretrovirus/virologia , Ensaio de Imunoadsorção Enzimática , Haplorrinos , Dados de Sequência Molecular , Doenças dos Macacos/virologia , Proteínas Mutantes/genética , Proteínas Mutantes/imunologia , RNA Viral/genética , Análise de Sequência de DNA , Vírus Linfotrópico T Tipo 1 de Símios/genéticaRESUMO
Simian T-lymphotropic virus type 1 (STLV-1) strains occasionally infect humans. However, the frequency of such infections is unknown. We show that direct transmission of STLV-1 from nonhuman primates to humans may be responsible for a substantial proportion of human T-lymphotropic virus type 1 infections in rural Côte d'Ivoire, where primate hunting is common.
Assuntos
Infecções por HTLV-I/transmissão , Vírus Linfotrópico T Tipo 1 Humano/genética , Animais , Côte d'Ivoire , Genes env , Vírus Linfotrópico T Tipo 1 Humano/imunologia , Humanos , Filogenia , Primatas , Vírus Linfotrópico T Tipo 1 de Símios/genética , Vírus Linfotrópico T Tipo 1 de Símios/imunologia , Sequências Repetidas TerminaisRESUMO
We used mandrills (Mandrillus sphinx) naturally infected with simian T-cell leukemia virus type 1 (STLV-1) as a model for evaluating the influence of natural STLV-1 infection on the dynamics and evolution of the immune system during chronic infection. Furthermore, in order to evaluate the role of the immune system in controlling the infection during latency, we induced immunosuppression in the infected monkeys. We first showed that the STLV-1 proviral load was higher in males than in females and increased significantly with the duration of infection: mandrills infected for 10-6 years had a significantly higher proviral load than those infected for 2-4 years. Curiously, this observation was associated with a clear reduction in CD4+ T-cell number with age. We also found that the percentage of CD4(+) T cells co-expressing the activation marker HLA-DR and the mean percentage of CD25(+) in CD4(+) and CD8(+) T cells were significantly higher in infected than in uninfected animals. Furthermore, the STLV-1 proviral load correlated positively with T-cell activation but not with the frequency of T cells secreting interferon gamma in response to Tax peptides. Lastly, we showed that, during immunosuppression in infected monkeys, the percentages of CD8(+) T cells expressing HLA-DR(+) and of CD4(+) T cells expressing the proliferation marker Ki67 decreased significantly, although the percentage of CD8(+) T cells expressing HLA-DR(+) and Ki67 increased significantly by the end of treatment. Interestingly, the proviral load increased significantly after immunosuppression in the monkey with the highest load. Our study demonstrates that mandrills naturally infected with STLV-1 could be a suitable model for studying the relations between host and virus. Further studies are needed to determine whether the different compartments of the immune response during infection induce the long latency by controlling viral replication over time. Such studies would provide important information for the development of immune-based therapeutic strategies.
Assuntos
Imunossupressores/uso terapêutico , Síndrome de Imunodeficiência Adquirida dos Símios/imunologia , Síndrome de Imunodeficiência Adquirida dos Símios/virologia , Vírus Linfotrópico T Tipo 1 de Símios/imunologia , Linfócitos T/imunologia , Linfócitos T/virologia , Carga Viral , Animais , Linfócitos T CD4-Positivos/metabolismo , Linfócitos T CD4-Positivos/virologia , Linfócitos T CD8-Positivos/metabolismo , Linfócitos T CD8-Positivos/virologia , Feminino , Citometria de Fluxo/métodos , Antígenos HLA-DR/metabolismo , Interferon gama/metabolismo , Subunidade alfa de Receptor de Interleucina-2/biossíntese , Masculino , Mandrillus , Tacrolimo/uso terapêuticoRESUMO
BACKGROUND: Although a wide variety of non-human primates are susceptible to simian T-cell leukaemia virus type 1 (STLV-1), little is known about the virological or molecular determinants of natural STLV-1 infection. METHODS: We determined STLV-1 virus tropism in vivo and its relation to the immune response by evaluating cytokine production and T-cell subsets in naturally infected and uninfected mandrills. RESULTS: With real-time PCR methods, we found that STLV-1 in mandrills infects both CD4(+) and CD8(+) T cells; however, proviral loads were significantly higher (P = 0.01) in CD4(+) than in CD8(+) cells (mean STLV-1 copies number per 100 cells (+/- SD) was 7.8 +/- 8 in CD4(+) T cells and 3.9 +/- 4.5 in CD8(+) T cells). After culture, STLV-1 provirus was detected in enriched CD4(+) but not in enriched CD8(+) T cells. After 6 months of culture, STLV-1-transformed cell lines expressing CD3(+), CD4(+) and HLADR(+) were established, and STLV-1 proteins and tax/rex mRNA were detected. In STLV-1 infected monkeys, there was a correlation between high proviral load and elevated levels of interleukin (IL)-2, IL-6, IL-10, interferon-gamma and tumour necrosis factor-alpha. The two monkeys with the highest STLV-1 proviral load had activated CD4(+)HLADR(+) and CD8(+)HLADR(+) T-cell subsets and a high percentage of CD25(+) in CD4(+) and CD8(+) T cells. CONCLUSIONS: Our study provides the first cellular, immunological and virological characterization of natural STLV-1 infection in mandrills and shows that they are an appropriate animal model for further physiopathological studies of the natural history of human T-cell leukaemia viruses.
Assuntos
Citocinas/metabolismo , Mandrillus , Vírus Linfotrópico T Tipo 1 de Símios/imunologia , Linfócitos T/fisiologia , Animais , Anticorpos , Células Cultivadas , Doença Crônica , Perfilação da Expressão Gênica , Regulação Viral da Expressão Gênica/fisiologia , Reação em Cadeia da Polimerase/veterinária , Linfócitos T/citologia , Carga ViralRESUMO
Specific pathogen-free (SPF) macaque colonies are now requested frequently as a resource for research. Such colonies were originally conceived as a means to cull diseased animals from research-dedicated colonies, with the goal of eliminating debilitating or fatal infectious agents from the colony to improve the reproductive capacity of captive research animals. The initial pathogen of concern was Mycobacterium tuberculosis (M.tb.), recognized for many years as a pathogen of nonhuman primates as well as a human health target. More recently attention has focused on four viral pathogens as the basis for an SPF colony: simian type D retrovirus (SRV), simian immunodeficiency virus (SIV), simian T cell lymphotropic/leukemia virus (STLV), and Cercopithecine herpesvirus 1 (CHV-1). New technologies, breeding, and maintenance schemes have emerged to develop and provide SPF primates for research. In this review we focus on the nonhuman primates (NHPs) most common to North American NHP research facilities, Asian macaques, and the most common current research application of these animals, modeling of human AIDS.
Assuntos
Macaca/sangue , Organismos Livres de Patógenos Específicos , Animais , Herpesvirus Cercopitecino 1/crescimento & desenvolvimento , Herpesvirus Cercopitecino 1/imunologia , Macaca/microbiologia , Macaca/virologia , Mycobacterium tuberculosis/crescimento & desenvolvimento , Mycobacterium tuberculosis/imunologia , Vírus da Imunodeficiência Símia/crescimento & desenvolvimento , Vírus da Imunodeficiência Símia/imunologia , Vírus Linfotrópico T Tipo 1 de Símios/crescimento & desenvolvimento , Vírus Linfotrópico T Tipo 1 de Símios/imunologiaRESUMO
Humans and simian species are infected by deltaretroviruses (HTLV and STLV respectively), which are collectively called primate T-cell lymphotropic viruses (PTLVs). In humans, four types of HTLV have been described (HTLV-1 to -4) with three of them having closely related simian virus analogues named STLV-1, 2 and 3. In this study, our aim was to search for a simian HTLV-4-related virus and to document and characterize further the diversity of STLV infections in wild primate populations. We screened 1297 whole blood samples from 13 different primate species from southern Cameroon. Overall, 93 samples gave HTLV-1, HTLV-2 or dual HTLV-1/-2 INNOLIA profiles, 12 were HTLV positive but untypeable and 14 were indeterminate. Subsequently, we performed generic and specific (STLV-1 to -3) tax-rex PCRs to discriminate the different PTLV types, completed with phylogenetic analysis of 450-bp LTR sequences for STLV-1 and 900 bp pX-LTR sequences for STLV-3. We show for the first time that Lophocebus albigena and Cercopithecus cephus carry both STLV-1 and a divergent STLV-3. We also identified a new STLV-1 lineage in one C. cephus. Finally, we identify relative divergence levels in the tax/rex phylogeny suggesting that additional types of PTLV should be defined, particularly for the highly divergent STLV-1(MarB43) strain that we provisionally name STLV-5.
Assuntos
Animais Selvagens/virologia , Doenças dos Símios Antropoides/virologia , Infecções por Deltaretrovirus/veterinária , Doenças dos Macacos/virologia , Vírus Linfotrópico T Tipo 3 de Primatas/classificação , Vírus Linfotrópico T Tipo 1 de Símios/classificação , Animais , Anticorpos Antivirais/sangue , Doenças dos Símios Antropoides/epidemiologia , Camarões , Cercocebus , Infecções por Deltaretrovirus/epidemiologia , Infecções por Deltaretrovirus/virologia , Variação Genética , Gorilla gorilla , Haplorrinos , Dados de Sequência Molecular , Doenças dos Macacos/epidemiologia , Filogenia , Vírus Linfotrópico T Tipo 3 de Primatas/genética , Vírus Linfotrópico T Tipo 3 de Primatas/imunologia , Vírus Linfotrópico T Tipo 3 de Primatas/isolamento & purificação , Análise de Sequência de DNA , Vírus Linfotrópico T Tipo 1 de Símios/genética , Vírus Linfotrópico T Tipo 1 de Símios/imunologia , Vírus Linfotrópico T Tipo 1 de Símios/isolamento & purificação , Sequências Repetidas Terminais/genéticaRESUMO
BACKGROUND: The breeding colony of free-ranging rhesus macaques was established in 1938 in Cayo Santiago (CS) with animals collected in northern India. The seroprevalence to cercopithecine herpesvirus type 1 (B virus) and simian retroviruses has been studied previously. RESULTS: This is the first report on the seropositivity to different viruses using samples collected shortly after removing animals (n = 245) from CS. All samples were negative for measles, simian immunodeficiency virus and simian type D retroviruses. The overall prevalence of antibodies was around 50% for simian T-lymphotropic virus I (STLV-I). For B virus, the prevalence was 38%. CONCLUSIONS: Data obtained showed marked differences in the antibody distribution to B virus and STLV-I within the free-ranging colony of rhesus macaques. Implication of these data for the Specific Pathogen Free program at the Caribbean Primate Research Center are also discussed.
Assuntos
Anticorpos Antivirais/sangue , Macaca mulatta/sangue , Macaca mulatta/imunologia , Envelhecimento , Animais , Região do Caribe , Herpesvirus Cercopitecino 1/imunologia , Estudos Soroepidemiológicos , Caracteres Sexuais , Vírus Linfotrópico T Tipo 1 de Símios/imunologiaRESUMO
Our goal was to determine if a multiplex technique using a fluorescent bead-based flow cytometric assay could yield results comparable to traditional enzyme-linked immunosorbent assay (ELISA) in terms of sensitivity, specificity, cross-reactivity, and throughput. We applied both techniques to serologic screening of specific pathogen-free macaques, for type D simian retrovirus, simian T-lymphotropic virus, Cercopithicine herpesvirus 1, and simian immunodeficiency virus, and found a high correlation between the bead-based multiplex assay and ELISA. The multiplex assay demonstrated greater sensitivity with no loss in specificity when compared to the ELISA. A lower false-positive rate with the multiplex assay decreased the number of confirmatory Western blots required. Using the multiplex assay, we were able to screen samples for 4 viruses simultaneously in the time it took to perform a single-virus ELISA, resulting in a faster turnaround time and higher throughput. The multiplexed assay provided greater sensitivity, increased stability, and better performance than ELISA.
Assuntos
Anticorpos Antivirais/sangue , Citometria de Fluxo/métodos , Viroses/diagnóstico , Viroses/imunologia , Vírus/imunologia , Animais , Ensaio de Imunoadsorção Enzimática , Citometria de Fluxo/instrumentação , Herpesvirus Cercopitecino 1/imunologia , Imunoglobulina G/sangue , Microesferas , Retrovirus dos Símios/imunologia , Sensibilidade e Especificidade , Vírus da Imunodeficiência Símia/imunologia , Vírus Linfotrópico T Tipo 1 de Símios/imunologia , Organismos Livres de Patógenos Específicos , Viroses/virologiaRESUMO
A diagnosis of glioblastoma multiforme (GBM) was made for cerebral masses found at necropsy in two baboons (Papio cynocephalus anubis). Case 1 was an adult (6.18 years old) male baboon that suddenly died during a physical examination as part of a clinical evaluation for a leg lameness. Case 2 was an adult (5.95 years old) female baboon that stopped breathing during anesthesia for an magnetic resonance imaging to evaluate lethargy, weight loss, inappetence, and dilated pupils. Both animals had undergone total body irradiation with cobalt during a research protocol. The incidence of spontaneous brain tumors in nonhuman primates is low, but radiation-induced GBM lesions in rhesus macaques (Macaca mulatta) have been reported. A definitive diagnosis was made in these cases, using histopathologic criteria of cellular pleomorphism, high mitotic rate, regions of coagulation necrosis, and endothelial proliferation.
Assuntos
Neoplasias Encefálicas/veterinária , Glioblastoma/veterinária , Neoplasias Induzidas por Radiação/veterinária , Doenças dos Primatas/patologia , Animais , Anticorpos Antivirais/análise , Transplante de Medula Óssea , Neoplasias Encefálicas/etiologia , Neoplasias Encefálicas/patologia , Feminino , Glioblastoma/etiologia , Glioblastoma/patologia , Coxeadura Animal , Masculino , Papio , Vírus Linfotrópico T Tipo 1 de Símios/imunologia , Vírus Linfotrópico T Tipo 1 de Símios/isolamento & purificaçãoRESUMO
Simian T-cell leukemia viruses (STLVs) are the simian counterparts of human T-cell leukemia viruses (HTLVs). A novel, divergent type of STLV (STLV-L) from captive baboons was reported in 1994, but its natural prevalence remained unclear. We investigated the prevalence of STLV-L in 519 blood samples from wild-living nonhuman primates in Ethiopia. Seropositive monkeys having cross-reactive antibodies against HTLV were found among 22 out of 40 hamadryas baboons, 8 of 96 anubis baboons, 24 of 50 baboons that are hybrids between hamadryas and anubis baboons, and 41 of 177 grivet monkeys, but not in 156 gelada baboons. A Western blotting assay showed that sera obtained from seropositive hamadryas and hybrid baboons exhibited STLV-L-like reactivity. A PCR assay successfully amplified STLV sequences, which were subsequently sequenced and confirmed as being closely related to STLV-L. Surprisingly, further PCR showed that nearly half of the hamadryas (20 out of 40) and hybrid (19 out of 50) baboons had STLV-L DNA sequences. In contrast, most of the seropositive anubis baboons and grivet monkeys carried typical STLV-1 but not STLV-L. These observations demonstrate that STLV-L naturally prevails among hamadryas and hybrid baboons at significantly high rates. STLV-1 and -2, the close relative of STLV-L, are believed to have jumped across simian-human barriers, which resulted in widespread infection of HTLV-1 and -2. Further studies are required to know if STLV-L is spreading into human populations.
Assuntos
Anticorpos Antivirais/sangue , Infecções por Deltaretrovirus/veterinária , Doenças dos Macacos/epidemiologia , Papio , Vírus Linfotrópico T Tipo 1 de Símios/imunologia , Animais , Animais Selvagens , Sequência de Bases , Reações Cruzadas , Infecções por Deltaretrovirus/epidemiologia , Infecções por Deltaretrovirus/virologia , Anticorpos Anti-HTLV-I/sangue , Anticorpos Anti-HTLV-II/sangue , Humanos , Dados de Sequência Molecular , Doenças dos Macacos/virologia , Filogenia , Prevalência , Análise de Sequência de DNA , Vírus Linfotrópico T Tipo 1 de Símios/genética , Vírus Linfotrópico T Tipo 1 de Símios/isolamento & purificaçãoRESUMO
A serological survey searching for antibodies reacting with human T-cell leukemia virus type 1 (HTLV-1) antigens was performed on a series of 263 sera/plasma obtained from 34 monkey species or subspecies, originating from different parts of Africa. Among them, 34 samples exhibited a typical HTLV-1 Western blot pattern. Polymerase chain reaction was performed with three primer sets specific either to HTLV-1/STLV-1 or HTLV-2 and encompassing gag, pol, and tax sequences, on genomic DNA from peripheral blood mononuclear cells of 31 animals. The presence of HTLV-1/simian T-cell leukemia virus type 1 (STLV-1) related viruses was determined in the 21 HTLV-1 seropositive animals tested but not in the 10 HTLV-1 seronegative individuals. Proviral DNA sequences from the complete LTR (750 bp) and a portion of the env gene (522 bp) were determined for 16 new STLV-1 strains; some of them originating from species for which no STLV-1 molecular data were available as Allenopithecus nigroviridis and Cercopithecus nictitans. Comparative and phylogenetic analyses revealed that these 16 new sequences belong to five different molecular groups. The A. nigroviridis STLV-1 strains exhibited a very strong nucleotide similarity with HTLV-1 of the subtype B. Furthermore, four novel STLV-1, found in Cercocebus torquatus, C. m. mona, C. nictitans, and Chlorocebus aethipos, were identical to each other and to a previously described Papio anubis STLV-1 strain (PAN 503) originating from the same primate center in Cameroon. Our data extend the range of the African primates who could be permissive and/or harbor naturally STLV-1 and provide new evidences of cross-transmission of African STLV-1 between different monkey species living in the same environment and also of STLV-1 transmissions from some monkeys to humans in Central Africa.
Assuntos
Cercopithecinae/virologia , Vírus Linfotrópico T Tipo 1 de Símios/classificação , África , Animais , DNA Viral/análise , Produtos do Gene env/genética , Vírus Linfotrópico T Tipo 1 Humano/classificação , Vírus Linfotrópico T Tipo 1 Humano/genética , Vírus Linfotrópico T Tipo 1 Humano/imunologia , Filogenia , Proteínas Oncogênicas de Retroviridae/genética , Análise de Sequência de DNA , Vírus Linfotrópico T Tipo 1 de Símios/genética , Vírus Linfotrópico T Tipo 1 de Símios/imunologia , Sequências Repetidas Terminais/genética , Produtos do Gene env do Vírus da Imunodeficiência HumanaRESUMO
STLV-1 viruses are closely related to HTLV-1 and infect many African monkey species. Seroreactivities of monkeys infected by STLV-1 are nearly identical to those of HTLV-1-infected individuals. In some cases, STLV-1 are, sequence-wise, indistinguishable from HTLV-1, and cannot be separated from them on the basis of phylogenetic analyses. HTLV-2-related simian viruses have been rarely reported. Such STLV-2 viruses, present in African bonobo (Pan paniscus), possess a genomic organization related to but different from all known HTLV-2 subtypes. We report here the molecular characterization and the subtyping of a new STLV-1 in a wild-caught baboon (Papio anubis) whose serum exhibited an indeterminate STLV-2-like serology (p24, GD21, MTA-1 with no p19). In the env and LTR regions, this virus is phylogenetically related to the large African STLV-1 group, but does not cluster with any STLV-1 baboon sequence. The complete p19 sequence reveals amino acid changes at critical positions. This is the first report of an African STLV-1 virus leading to an STLV-2-like serological profile in its host.
Assuntos
Doenças dos Macacos/virologia , Papio/virologia , Filogenia , Infecções por Retroviridae/veterinária , Vírus Linfotrópico T Tipo 1 de Símios/classificação , Sequência de Aminoácidos , Animais , Animais Selvagens , Anticorpos Antivirais/sangue , Antígenos Virais/química , Epitopos , Dados de Sequência Molecular , Infecções por Retroviridae/imunologia , Infecções por Retroviridae/virologia , Vírus Linfotrópico T Tipo 1 de Símios/genética , Vírus Linfotrópico T Tipo 1 de Símios/imunologiaRESUMO
Retroviruses closely related to the human T-cell leukaemia/lymphotrophic virus type I (HTLV-I) have been detected in several, non-human, primate species. These retroviruses are called simian T-lymphotrophic virus type I (STLV-I). Infection with STLV-I has been associated with lymphoma and leukaemia in macaques, baboons, African green monkeys and gorillas. However, no STLV-I infection has been detected in New World primates, although STLV-II has been detected in spider monkeys. When sera from 10 species of non-human primates maintained at the Institute of Primate Research were screened for STLV-I infection, anti-STLV-I antibodies were detected in 12%, 12%, 23% and 38% of the olive baboons, yellow baboons, African green monkeys and lowland Sykes' monkeys, respectively. Western-blot studies confirmed these results. To date, no clinical disease has been linked with STLV-I infection in these colonies. The relatively high prevalence of anti-STLV-I antibodies in these non-human primates offers an opportunity for studies on the transmission, phylogenetic relationships and natural history of STLV-I in primate colonies.
Assuntos
Anticorpos Antivirais/sangue , Primatas/imunologia , Vírus Linfotrópico T Tipo 1 de Símios/imunologia , Animais , Western Blotting , Chlorocebus aethiops/imunologia , Quênia , Papio/imunologia , Prevalência , Primatas/virologiaRESUMO
Lymphoma was seen in an 11-year-old female savanna monkey (Ceropithecus aethiops). The superficial inguinal and visceral lymph nodes were markedly enlarged, and their architecture was completely effaced by neoplastic cells. The neoplastic cells, which were highly pleomorphic, resembled those in adult T-cell lymphoma-leukemia in humans. Ultrastructurally the neoplastic cells were characterized by nuclear irregularity and clustered dense bodies, and almost all cells showed positivity for CD3. The animal had been reared with her family, and her mother and 2 brothers had antibodies reactive to human T-cell leukemia virus. This virus serologically cross-reacts with simian T-cell leukemia virus, which may be the causative agent of the present neoplasm.
Assuntos
Chlorocebus aethiops , Infecções por Deltaretrovirus/veterinária , Linfonodos/patologia , Linfoma de Células T/veterinária , Doenças dos Macacos/virologia , Vírus Linfotrópico T Tipo 1 de Símios , Animais , Infecções por Deltaretrovirus/patologia , Feminino , Linfoma de Células T/patologia , Linfoma de Células T/virologia , Doenças dos Macacos/patologia , Vírus Linfotrópico T Tipo 1 de Símios/imunologiaRESUMO
Evidence for the presence of simian T-lymphotropic viruses (STLV-I) was identified in live-caught pig-tailed macaques from two locations in southern Sumatra, Indonesia. Of 60 animals tested, 13.3% of the animals showed seroreactivity to HTLV-I/II enzyme-linked immunosorbent assay (ELISA) antigens. Of these, 75% showed indeterminate reactivity and 25% showed positive reactivity to HTLV-I/II Western blot antigens. Polymerase chain reaction (PCR) analysis of 6 of 8 seroreactive monkeys' peripheral blood mononuclear cell (PBMC) DNA showed production of proper size molecular weight product that hybridized specifically to an STLV-I tax gene-specific probe. Phylogenic analyses of tax gene fragment sequences from the PCR products of two samples, 930287 and 930306, indicated that these animals were infected with retroviruses related to those of the Asian STLV-I clade.
Assuntos
Infecções por Deltaretrovirus/veterinária , Macaca nemestrina , Doenças dos Macacos/epidemiologia , Vírus Linfotrópico T Tipo 1 de Símios , Animais , Anticorpos Antivirais/sangue , Sequência de Bases , Western Blotting/veterinária , DNA Viral/sangue , DNA Viral/química , Infecções por Deltaretrovirus/epidemiologia , Ensaio de Imunoadsorção Enzimática/veterinária , Feminino , Genótipo , Indonésia/epidemiologia , Masculino , Dados de Sequência Molecular , Filogenia , Reação em Cadeia da Polimerase/veterinária , Prevalência , Vírus Linfotrópico T Tipo 1 de Símios/classificação , Vírus Linfotrópico T Tipo 1 de Símios/genética , Vírus Linfotrópico T Tipo 1 de Símios/imunologiaRESUMO
A serological survey of 22 wild-caught South African (Transvaal) chacma baboons (Papio ursinus) and eight olive baboons (Papio anubis) from Kenya indicates that 13 P. ursinus and one P. anubis have antibodies reacting with human T cell leukemia/lymphoma virus type 1 (HTLV-1) antigens, whereas three P. ursinus had a indeterminate reactivity on Western blot analysis. With six primer sets specific to either HTLV-1-Simian T-cell leukemia virus type 1 (STLV-1) or HTLV-2 and encompassing long terminal repeat (LTR), gag, pol, env, and tax sequences, polymerase chain reaction was performed on genomic DNA from peripheral blood mononuclear cells of 18 animals, and the presence of HTLV-1-STLV-1-related viruses was determined in 13 seropositive and three seroindeterminate animals but not in the two HTLV seronegative individuals. Proviral DNA sequences from env (522 bp), pol (120 bp), and complete (755 bp) or partial (514 bp) LTR were determined for three STLV-1-infected P. ursinus and one P. anubis. Comparative and phylogenetic analyses revealed that P. anubis (Pan-486) sequence clusters with one (Pan-1621) of two previously described P. anubis STLV-1. Likewise, P. ursinus viruses (Pur-529, Pur-539, and Pur-543) form a distinct group, different from all known HTLV-1 but closely affiliated with two STLV-1 strains from South African vervets (Cercopithecus aethiops pygerythrus). This study, reporting the first STLV-1 sequences from wild-caught P. ursinus and P. anubis, corroborates the hypothesis of cross-species transmissions of STLV-1 in the wild. Further, phylogenetic analyses indicate that the known HTLV-1 strains do not share a common origin with nonhuman primates STLV in South Africa.