RESUMO
Partly due to climate change, and partly due to changes of human habitat occupation, the impact of tick-borne viruses is increasing. Nairobi sheep disease virus (NSDV) and Ganjam virus (GV) are two names for the same virus, which causes disease in sheep and goats and is currently known to be circulating in India and East Africa. The virus is transmitted by ixodid ticks and causes a severe hemorrhagic disease. We have developed a real-time PCR assay for the virus genome and validated it in a pilot study of the pathogenicity induced by two different isolates of NSDV/GV. One isolate was highly adapted to tissue culture, grew in most cell lines tested, and was essentially apathogenic in sheep. The second isolate appeared to be poorly adapted to cell culture and retained pathogenicity in sheep. The real-time PCR assay for virus easily detected 4 copies or less of the viral genome, and allowed a quantitative measure of the virus in whole blood. Measurement of the changes in cytokine mRNAs showed similar changes to those observed in humans infected by the closely related virus Crimean Congo hemorrhagic fever virus.
Assuntos
Citocinas/genética , Doenças das Cabras/genética , Doença dos Ovinos de Nairobi/genética , Vírus da Doença do Carneiro de Nairobi/patogenicidade , Reação em Cadeia da Polimerase em Tempo Real/métodos , Animais , Citocinas/metabolismo , Doenças das Cabras/imunologia , Doenças das Cabras/virologia , Cabras , Doença dos Ovinos de Nairobi/imunologia , Doença dos Ovinos de Nairobi/virologia , Vírus da Doença do Carneiro de Nairobi/classificação , Vírus da Doença do Carneiro de Nairobi/genética , Vírus da Doença do Carneiro de Nairobi/isolamento & purificação , RNA Viral/análise , Reação em Cadeia da Polimerase em Tempo Real/veterinária , Ovinos , Virulência , Replicação ViralRESUMO
Ganjam virus (GANV), a member of genus Nairovirus of family Bunyavirdae is of considerable veterinary importance in India. Though, predominantly tick borne, GANV was also isolated from mosquitoes, man and sheep. Neutralizing and complement fixing antibodies to GANV have been detected in animal and human sera collected from different parts of the country. Thirty three strains of GANV have been isolated from India, mainly from Haemaphysalis ticks. The virus replicated in certain vertebrate and mosquito cell lines and found pathogenic to laboratory animals. One natural infection and five laboratory-acquired infections in men were also reported. GANV is antigenically related to Nairobi sheep disease virus (NSDV) of Africa, which is highly pathogenic for sheep and goats causing 70-90 per cent mortality among the susceptible population. Recent molecular studies have demonstrated that GANV is an Asian variant of NSDV and both these viruses are related to the dreaded Crimean Congo haemorrhagic fever (CCHF) group viruses. The versatility of the virus to replicate in different arthropod species, its ability to infect sheep, goat and man makes it an important zoonotic agent.