RESUMO
With suppressed photon scattering and diminished autofluorescence, in vivo fluorescence imaging in the 1,500- to 1,700-nm range of the near-IR (NIR) spectrum (NIR-IIb window) can afford high clarity and deep tissue penetration. However, there has been a lack of NIR-IIb fluorescent probes with sufficient brightness and aqueous stability. Here, we present a bright fluorescent probe emitting at â¼1,600 nm based on core/shell lead sulfide/cadmium sulfide (CdS) quantum dots (CSQDs) synthesized in organic phase. The CdS shell plays a critical role of protecting the lead sulfide (PbS) core from oxidation and retaining its bright fluorescence through the process of amphiphilic polymer coating and transferring to water needed for imparting aqueous stability and compatibility. The resulting CSQDs with a branched PEG outer layer exhibited a long blood circulation half-life of 7 hours and enabled through-skin, real-time imaging of blood flows in mouse vasculatures at an unprecedented 60 frames per second (fps) speed by detecting â¼1,600-nm fluorescence under 808-nm excitation. It also allowed through-skin in vivo confocal 3D imaging of tumor vasculatures in mice with an imaging depth of â¼1.2 mm. The PEG-CSQDs accumulated in tumor effectively through the enhanced permeation and retention effect, affording a high tumor-to-normal tissue ratio up to â¼32 owing to the bright â¼1,600-nm emission and nearly zero autofluorescence background resulting from a large â¼800-nm Stoke's shift. The aqueous-compatible CSQDs are excreted through the biliary pathway without causing obvious toxicity effects, suggesting a useful class of â¼1,600-nm emitting probes for biomedical research.
Assuntos
Corantes Fluorescentes , Imageamento Tridimensional/métodos , Microscopia Intravital/métodos , Microscopia de Fluorescência/métodos , Imagem Óptica/métodos , Pontos Quânticos , Adenocarcinoma/irrigação sanguínea , Adenocarcinoma/secundário , Animais , Neoplasias do Colo/patologia , Estabilidade de Medicamentos , Artéria Femoral/ultraestrutura , Veia Femoral/ultraestrutura , Corantes Fluorescentes/análise , Corantes Fluorescentes/farmacocinética , Corantes Fluorescentes/toxicidade , Meia-Vida , Membro Posterior/irrigação sanguínea , Microscopia Intravital/instrumentação , Chumbo/química , Camundongos , Camundongos Endogâmicos C57BL , Microscopia Confocal/instrumentação , Microscopia Confocal/métodos , Microscopia Eletrônica , Microscopia de Fluorescência/instrumentação , Imagem Óptica/instrumentação , Pontos Quânticos/análise , Pontos Quânticos/química , Pontos Quânticos/toxicidade , Sulfetos/química , Gravação em VídeoRESUMO
Changes in the muscular tissue after subcutaneous injection of autologous bone marrow multipotent mesenchymal stromal cells transfected with GFP gene and additionally stained with cell membrane dye Vybrant CM-Dil in the projection of ligated femoral vein were studied by light microscopy with luminescence. Stromal cells injected through the skin can appear not only in the damaged tissue where acceleration of regeneration processes is required, but also in intact structures located in superficial or deeper layers. In intact muscular tissue, stromal cells spreading in the perivascular tissue initiate inflammation and migration of macrophages, activate and even trigger sclerotic processes due to differentiation into connective tissue cells (fibroblasts) and stimulation of proliferation and collagen synthesis by host fibroblasts. Injected multipotent mesenchymal stromal cells are gradually phagocytized by macrophages.
Assuntos
Fibroblastos/patologia , Macrófagos/patologia , Transplante de Células-Tronco Mesenquimais/efeitos adversos , Células-Tronco Mesenquimais/citologia , Esclerose/patologia , Animais , Animais Endogâmicos , Diferenciação Celular , Veia Femoral/fisiopatologia , Veia Femoral/ultraestrutura , Fibroblastos/metabolismo , Genes Reporter , Proteínas de Fluorescência Verde/genética , Proteínas de Fluorescência Verde/metabolismo , Injeções Intramusculares , Macrófagos/metabolismo , Masculino , Células-Tronco Mesenquimais/metabolismo , Músculo Esquelético/metabolismo , Músculo Esquelético/patologia , Fagocitose , Plasmídeos/química , Plasmídeos/metabolismo , Ratos , Esclerose/etiologia , Esclerose/metabolismo , Transfecção , Transplante AutólogoRESUMO
OBJECTIVE: Bilateral large variant veins were encountered in the lower extremity. It was aimed to identify the structural characteristics of this rare case and then, regarding the structural features, to overview its formation process and denomination. MATERIAL AND METHOD: During the routine dissection of a 93-year-old male cadaver, bilateral large variant veins were found at the thigh. Valves of the veins were examined and evaluated together with the vascular wall histology. RESULTS: The variant vein was loosely attached to the sciatic nerve by fibrous tissue and had anastomoses with the popliteal vein in the popliteal fossa on each side. The popliteal veins were hypoplastic on both sides. The right variant vein was passing through the fibers of the adductor magnus muscle 56.2 mm above the adductor hiatus, which corresponds to the third perforating branch of deep femoral vein. The left one was turning to the front over the adductor magnus muscle, at the lower border of quadratus femoris muscle. The left variant vein was corresponding to the descending branch of the medial circumflex femoral vein. Both variant veins had one incomplete and three well-developed valves. CONCLUSION: In accordance with the findings, the variant vein was concluded to be an embryonic remnant, rather than an acquired one subsequent to any obstruction of the femoral vein. Regarding their connection with the popliteal vein but not with the internal iliac vein, both variant veins were denominated as "lower type persistent sciatic vein". Such a variation would be important with respect to the risk of complication during popliteal sciatic nerve blockade.
Assuntos
Veia Femoral/anormalidades , Perna (Membro)/irrigação sanguínea , Veia Poplítea/anormalidades , Idoso de 80 Anos ou mais , Cadáver , Dissecação , Veia Femoral/ultraestrutura , Humanos , Artéria Ilíaca , Masculino , Veia Poplítea/ultraestruturaRESUMO
OBJECTIVE: Constriction of vein grafts with braided external nitinol meshes had previously led to the successful elimination of neointimal tissue formation. We investigated whether pulse compliance, smaller kink-free bending radius, and milder medial atrophy can be achieved by knitting the meshes rather than braiding, without losing the suppressive effect on intimal hyperplasia. METHODS: Pulse compliance, bending stiffness, and bending radius, as well as longitudinal-radial deformation-coupling and radial compression, were compared in braided and knitted nitinol meshes. Identical to previous studies with braided mesh grafts, a senescent nonhuman primate model (Chacma baboons; bilateral femoral interposition grafts/6 months) mimicking the clinical size mismatch between vein grafts and runoff arteries was used to examine the effect of knitted external meshes on vein grafts: nitinol mesh-constricted (group 1); nitinol mesh-constricted and fibrin sealant (FS) spray-coated for mesh attachment (group 2); untreated control veins (group 3), and FS spray-coated control veins (group 4). RESULTS: Compared with braided meshes, knitted meshes had 3.8-times higher pulse compliance (3.43 ± 0.53 vs 0.94 ± 0.12%/100 mm Hg; P = .00002); 30-times lower bending stiffness (0.015 ± 0.002 vs 0.462 ± 0.077 Nmm(2); P = .0006); 9.2-times narrower kink-free bending radius (15.3 ± 0.4 vs 140.8 ± 22.4 mm; P = .0006), and 4.3-times lower radial narrowing caused by axial distension (18.0% ± 1.0% vs 77.0% ± 3.7%; P = .00001). Compared with mesh-supported grafts, neointimal tissue was 8.5-times thicker in group I (195 ± 45 µm) vs group III (23.0 ± 21.0 µm; P < .001) corresponding with a 14.3-times larger neointimal area in group I (4330 ± 957 × 103 µm(2)) vs group III (303 ± 221× 103 µm(2); P < .00004). FS had no significant influence. Medial muscle mass remained at 43.4% in knitted meshes vs the 28.1% previously observed in braided meshes. CONCLUSION: Combining the suppression of intimal hyperplasia with a more physiologic remodeling process of the media, manifold higher kink-resistance, and lower fraying than in braided meshes makes knitted nitinol an attractive concept in external vein graft protection.
Assuntos
Ligas , Artéria Femoral/cirurgia , Veia Femoral/transplante , Telas Cirúrgicas , Enxerto Vascular/instrumentação , Animais , Fenômenos Biomecânicos , Complacência (Medida de Distensibilidade) , Desenho de Equipamento , Artéria Femoral/fisiopatologia , Artéria Femoral/ultraestrutura , Veia Femoral/fisiopatologia , Veia Femoral/ultraestrutura , Adesivo Tecidual de Fibrina , Hiperplasia , Teste de Materiais , Microscopia Eletrônica de Varredura , Modelos Animais , Papio ursinus , Fluxo Pulsátil , Fatores de Tempo , Enxerto Vascular/efeitos adversosRESUMO
The adductor canal is a conical or pyramid-shaped pathway that contains the femoral vessels, saphenous nerve and a varying amount of fibrous tissue. It is involved in adductor canal syndrome, a claudication syndrome involving young individuals. Our objective was to study modifications induced by aging on the connective tissue and to correlate them to the proposed pathophysiological mechanism. The bilateral adductor canals and femoral vessels of four adult and five fetal specimens were removed en bloc and analyzed. Sections 12 microm thick were obtained and the connective tissue studied with Sirius Red, Verhoeff, Weigert and Azo stains. Scanning electron microscopy (SEM) photomicrographs of the surfaces of each adductor canal were also analyzed. Findings were homogeneous inside each group. The connective tissue of the canal was continuous with the outer layer of the vessels in both groups. The pattern of concentric, thick collagen type I bundles in fetal specimens was replaced by a diffuse network of compact collagen bundles with several transversal fibers and an impressive content of collagen III fibers. Elastic fibers in adults were not concentrated in the thick bundles but dispersed in line with the transversal fiber system. A dynamic compression mechanism with or without an evident constricting fibrous band has been proposed previously for adductor canal syndrome, possibly involving the connective tissue inside the canal. The vessels may not slide freely during movement. These age-related modifications in normal individuals may represent necessary conditions for this syndrome to develop.
Assuntos
Tecido Conjuntivo/embriologia , Artéria Femoral/embriologia , Veia Femoral/embriologia , Coxa da Perna/embriologia , Adulto , Colágeno/ultraestrutura , Tecido Conjuntivo/ultraestrutura , Tecido Elástico/embriologia , Tecido Elástico/ultraestrutura , Feminino , Artéria Femoral/ultraestrutura , Veia Femoral/ultraestrutura , Feto/ultraestrutura , Humanos , Masculino , Microscopia Eletrônica , Microscopia Eletrônica de Varredura , Microscopia de Polarização , Pessoa de Meia-Idade , Coxa da Perna/anatomia & histologia , Coxa da Perna/irrigação sanguínea , Adulto JovemRESUMO
The arteriovenous loop (AV loop) model is gaining importance as a means of initiating and sustaining perfusion in tissue engineering constructs in vivo. This study represents an attempt to dissect the morphology of early arterialization and angiogenesis in the AV loop in a fibrin matrix with special focus on the interpositional venous graft (IVG) segment. An AV loop was constructed in 30 rats using the femoral vessels and an IVG. The AV loop was encased in an isolation chamber filled with a fibrin matrix. Evaluation methods included scanning electron microscopy (SEM) of corrosion casts, immune histology and micro magnetic resonance angiography (MRA). Direct luminal neovascular sprouting was evident between day 10 and day 14 from the vein and the IVG but not from the arterial segment. Arterialization of the IVG manifested itself on the corrosion casts as a gradual reduction in luminal caliber with onset after day 7. Microdissection of the microvascular replicas could demonstrate for the first time the presence of direct luminal sprouts from the IVG. MRA was used to display the shunt pattern of perfusion in the patent AV loop. From the three segments of the vascular axis in the AV loop the IVG is the most versatile for applications in the clinical as well as the experimental setting. Kinetics of angiogenesis warrant further investigation in the IVG.
Assuntos
Derivação Arteriovenosa Cirúrgica , Artéria Femoral/metabolismo , Veia Femoral/metabolismo , Adesivo Tecidual de Fibrina/metabolismo , Neovascularização Patológica/metabolismo , Engenharia Tecidual/métodos , Animais , Pressão Sanguínea , Molde por Corrosão , Artéria Femoral/fisiopatologia , Artéria Femoral/cirurgia , Artéria Femoral/ultraestrutura , Veia Femoral/fisiopatologia , Veia Femoral/cirurgia , Veia Femoral/ultraestrutura , Imuno-Histoquímica , Angiografia por Ressonância Magnética , Masculino , Microdissecção , Microscopia Eletrônica de Varredura , Neovascularização Patológica/patologia , Neovascularização Patológica/fisiopatologia , Ratos , Ratos Endogâmicos Lew , Fluxo Sanguíneo Regional , Estresse Mecânico , Fatores de Tempo , Grau de Desobstrução VascularRESUMO
OBJECTIVE: Abbe and Payr introduced vascular techniques and devices to facilitate vessel anastomosis over a century ago. Obora published the idea of a sutureless vascular anastomosis with use of magnetic rings in 1978. The purpose of this study was to assess the performance of a new magnetic device to perform a side-to-side arteriovenous anastomosis in a dog model. MATERIAL AND METHODS: Male fox hounds (25 kg) were treated preoperatively and daily postoperatively with clopidogrel bisulfate (Plavix) and aspirin. The femoral artery and vein were exposed unilaterally in 3 dogs and bilaterally in 4 dogs (n = 11 anastomoses). A 4-mm arteriotomy was performed, and 1 oval magnet 0.5 mm thick was inserted into the lumen of the artery and a second magnet was applied external to the artery, compressing and stabilizing the arterial wall to create a magnetic port. An identical venous magnetic port was created with another pair of oval magnets. When the 2 ports were allowed to approach each other, they self-aligned and magnetically coupled to complete the arteriovenous anastomosis. Patency was assessed for the first hour with direct observation, again after 9 weeks with duplex ultrasound scanning, and at 10 weeks under direct open observation. The anastomoses were explanted after 10 weeks. Hydrodynamic resistance was measured ex vivo on the final 8 anastomoses by measuring the pressure drop across an anastomosis with a known flow rate. RESULTS: After implantation, very high flow created visible turbulence and palpable vibration. All 11 anastomoses were patent under direct observation and palpation. Ten of 11 anastomoses were clearly patent on duplex scans, and patency of 1 anastomosis was questionable. Hydrodynamic resistance averaged 0.73 +/- 0.33 mm Hg min/mL (mean +/- SEM). CONCLUSIONS: Vascular anastomoses performed with magnets demonstrated feasibility; exhibited 100% patency after 10 weeks in a dog arteriovenous shunt model; lacked apparent aneurysm or other potentially catastrophic failure; demonstrated remodeling of the vessel wall after several weeks to incorporate the magnets, making the magnetic force unnecessary; and warrants further study in vessels with different sizes, flow rates, and locations. CLINICAL RELEVANCE: We present a magnet-based device used to perform side-to-side peripheral vascular anastomoses. Its advantages include the ability to anastomose vessels without requiring circumferential surgical exposure. Vascular anastomosis performed with these magnets demonstrated 100% patency in the dog, lacked apparent aneurysm or other potentially catastrophic failure, and demonstrated remodeling of the vessel wall after several weeks, to incorporate the magnets, making indefinite retention of field strength unnecessary. This technique could enable minimally invasive procedures, such as complex reconstructive and revascularizing surgery, and warrants further study in vessels with different sizes, flow rates, and locations.
Assuntos
Derivação Arteriovenosa Cirúrgica/instrumentação , Derivação Arteriovenosa Cirúrgica/métodos , Artéria Femoral/cirurgia , Veia Femoral/cirurgia , Magnetismo , Animais , Cães , Endotélio Vascular/ultraestrutura , Estudos de Viabilidade , Artéria Femoral/fisiopatologia , Artéria Femoral/ultraestrutura , Veia Femoral/fisiopatologia , Veia Femoral/ultraestrutura , Tecido de Granulação/ultraestrutura , Masculino , Teste de Materiais , Grau de Desobstrução Vascular , Resistência VascularRESUMO
The aim of this study was to document similarities and differences between veins from human diabetic patients and an experimentally induced diabetic animal model. The saphenous vein and posterior tibial vein from diabetic patients and the femoral vein from rats were studied. An increase in the extracellular matrix with migration of smooth muscle cells and endothelial alterations were observed in the intima of all specimens. These findings demonstrate that there is a high degree of similarity between the pathological changes in the venous wall during human diabetes mellitus and streptozotocin (STZ) induced-diabetes. This finding validates STZ induced-diabetes in rats as a model for further experimental study to clarify the fate of the diabetic venous wall when used as a graft.
Assuntos
Diabetes Mellitus Experimental/patologia , Diabetes Mellitus Tipo 1/patologia , Angiopatias Diabéticas/patologia , Túnica Íntima/ultraestrutura , Idoso , Animais , Feminino , Veia Femoral/ultraestrutura , Humanos , Masculino , Microscopia Eletrônica , Ratos , Ratos Sprague-Dawley , Veia Safena/ultraestruturaRESUMO
The commercially available mechanical vascular anastomotic device with ring-pin technique employs polyethylene rings and stainless steel pins, but even after healing of the anastomotic site, the ring remains. To resolve this disadvantage, the ring material was replaced by biodegradable material, and a ring coupling device was fabricated. This new ring was used to anastomose the femoral vein of rabbits, and morphological observations were made macroscopically and by light and scanning electron microscopy. Of the 58 anastomosed veins, 54 were patent, giving a patency rate of 92.9%. The ring was largely absorbed 15 weeks after anastomosis and completely after 30 weeks. Histologically, re-endothelialisation was observed in 1 week. Infiltration of inflammatory cells was significant around the ring at 3 weeks, but inflammation subsided within 6 weeks. There were no findings indicating adverse effects of the stainless steel pins remaining outside the vascular wall.
Assuntos
Implantes Absorvíveis , Anastomose Cirúrgica/instrumentação , Veia Femoral/cirurgia , Anastomose Cirúrgica/métodos , Animais , Endotélio Vascular/ultraestrutura , Estudos de Avaliação como Assunto , Veia Femoral/ultraestrutura , Microscopia Eletrônica de Varredura , Coelhos , Fatores de TempoRESUMO
UNLABELLED: In previous studies we have shown that 80-100% of rabbit femoral vascular autografts cold-stored at 4 degrees C for 3 weeks remain patent 3 weeks after reinsertion in the femoral artery. The present study reports the effect on graft patency of increasing either the period of cold storage prior to reinsertion or the duration of reperfusion to 6 months. Rabbit femoral blood vessels were cold-stored (CS) at 4 degrees C for varying periods. CS autografts were reinserted into the contralateral leg for 3 weeks or 6 months. Graft patency was determined and grafts examined by histological, immunohistochemical and electron microscopical techniques. Six months after reinsertion patency of 4-week CS arterial and 1-week CS venous grafts was 40% and 27% respectively, very much lower than the 80-100% seen after 3 weeks reperfusion. Arterial grafts CS for 6 months had a patency rate of 70% after 3 weeks reperfusion but 0% after 6 months. Morphological examination suggests that the delayed failure of cold-stored vascular grafts is caused by thrombus superimposed on intimal hyperplasia within the graft. CONCLUSIONS: Cold-stored vascular grafts are useful prostheses when only 3-4 weeks graft patency is required. They are not suitable for use when long-term graft patency is needed.
Assuntos
Prótese Vascular , Artéria Femoral/transplante , Veia Femoral/transplante , Preservação de Tecido , Grau de Desobstrução Vascular , Animais , Criopreservação , Artéria Femoral/ultraestrutura , Veia Femoral/ultraestrutura , Oclusão de Enxerto Vascular/patologia , Microscopia Eletrônica , Microscopia Eletrônica de Varredura , Período Pós-Operatório , Coelhos , Fatores de TempoRESUMO
1. We investigated the role of vascular smooth muscle alpha-adrenoceptor subtypes in the vasoconstrictor response of femoral arteries and veins to dopamine and whether the vasoconstriction is modified by endothelium-dependent relaxation mediated via the activation of alpha 2-adrenoceptors in ring preparations of femoral arteries and veins from mongrel dogs. 2. Dopamine contracted both arteries and veins in a dose-dependent manner and this contraction was inhibited by pretreatment with phentolamine or prazosin. Pretreatment with yohimbine shifted the dose-response curve for dopamine to the right in femoral veins, but not in arteries. 3. Phenylephrine contracted femoral arteries and veins in a dose-dependent manner and this contraction was inhibited by pretreatment with prazosin. 4. Clonidine produced a bell-shaped dose-response curve in femoral veins and this curve was shifted upwards by pretreatment with NG-nitro-L-arginine (L-NNA). In contrast, femoral arteries were not affected by clonidine. NG-Nitro-L-arginine potentiated contractile responses to dopamine in both veins and arteries. This potentiation was inhibited by yohimbine or by the removal of the endothelium in both arteries and veins. 5. These results suggest that dopamine contracts femoral arteries via stimulation of alpha 1-adrenoceptors and contracts femoral veins via stimulation of both alpha 1- and alpha 2-adrenoceptors and that these contractions are attenuated by the vasodilator action of dopamine via alpha 2-adrenoceptor-mediated release of endothelium-derived relaxing factor.
Assuntos
Dopamina/farmacologia , Artéria Femoral/efeitos dos fármacos , Artéria Femoral/fisiologia , Veia Femoral/efeitos dos fármacos , Veia Femoral/fisiologia , Óxido Nítrico/metabolismo , Receptores Adrenérgicos alfa 2/fisiologia , Agonistas alfa-Adrenérgicos/farmacologia , Antagonistas Adrenérgicos alfa/farmacologia , Animais , Clonidina/farmacologia , Cães , Interações Medicamentosas , Endotélio Vascular/fisiologia , Inibidores Enzimáticos/farmacologia , Artéria Femoral/ultraestrutura , Veia Femoral/ultraestrutura , Técnicas In Vitro , Contração Muscular/efeitos dos fármacos , Músculo Liso Vascular/efeitos dos fármacos , Músculo Liso Vascular/fisiologia , Músculo Liso Vascular/ultraestrutura , Nitroarginina/farmacologia , Receptores Adrenérgicos alfa 2/classificação , Vasoconstrição/efeitos dos fármacos , Vasoconstrição/fisiologiaRESUMO
The neovascularization of the arterial wall in human and experimental pathology has been demonstrated. The occlusion of the of the rat femoral artery is a suitable model for the study of these angiogenesis processes. Newly formed capillaries growing into the arterial wall have been described in this model. The origin of these ingrowing capillaries has been attribute to the preformed surrounding venules and capillaries. The contribution of the adjacent femoral vein with a supplementary population of vascular sprouts could also be possible. To test this hypothesis in half of the occluded arteries, the adventitia was removed from the side facing the femoral vein. Between 1 and 3 days after surgery several alterations were found both in the endothelial cells and the smooth muscle cells of the tunica media. Between 3 and 6 days, solid or canalized endothelial sprouts were observed arising from the femoral vein. By days 4 and 6, newly formed capillaries grew into the adventitia and tunica media of the femoral artery. Some of them, penetrated the internal elastic lamina. This microvascular penetration from the femoral vein was more prominent in the area of the ostium of the collateral and when the adventitia was removed. Some ingrowing capillaries were in continuity with the endothelial cells of the arterial neointima. At days 7 and 8, regressing capillaries were observed in the neomicrovasculature network between artery and vein, with a selective loss of the smaller vessels. From day 9 onwards, fewer and larger vascular channels were present between the femoral vein and the femoral artery. An arterial neolumen contained what appeared to be circulating "fresh" blood. Quantitatively, the venous neocapillary density increased from days 4 to 6 and then declined significantly by day 8. The arterial neocapillary density increased form days 4 to 8 and declined significantly by day 12. Moreover, both densities were significantly greater when the arterial adventitia was removed. The perfusion with barium solution showed the presence of the contrast material in the newly formed vessels, the lumen of the femoral vein, and the neolumen of the occluded arterial segment. The present findings indicate that putative angiogenic molecules released form the occluded arterial segment may reach the adjacent wall of the vein inducing neovascularization from it. The vein vascular sprouts are connected to the ingrowing capillaries in the occluded arterial wall and to the neocapillaries form the preexisting pericytic microvasculature. When the arterial adventitia were removed up to 2 times greater vein neocapillary's density was observed suggesting an easily access of the putative angiogenic factors to the vein.
Assuntos
Arteriosclerose Obliterante/patologia , Artéria Femoral/patologia , Veia Femoral/patologia , Neovascularização Patológica , Animais , Artéria Femoral/ultraestrutura , Veia Femoral/ultraestrutura , Humanos , Microcirculação/patologia , Microscopia Eletrônica , Ratos , Ratos Sprague-Dawley , Túnica Íntima/patologia , Túnica Íntima/ultraestruturaRESUMO
For the microscopical study of the great saphenous vein wall, the venous segments from six male necropsiedcadavers: were obtained three young individuals between 16 and 35 years old and three aged individuals between 50 and 83 years old. The tunica media of the great saphenous vein at the upper third level of the thigh belonging to both young and aged individuals is made up of two layers: an internal formed by fascicles of smooth longitudinal muscular fibers and the external layer made up by fascicles of smooth circular muscular fibers. At the level of the discharge in the femoral, the tunica media of the great saphenous vein wall of both, young and aged individuals, is made up of one layer, whose fascicles of smooth muscular fibers are circularly orientated in relation to the vascular axis. The tunica adventitia of the great saphenous vein wall at the upper third level of the young individuals thigh, presents elastic fibers whereas it the aged individuals it is made up of fascicles of smooth longitudinal fibers; at the level of the femoral vein disdarge in both young and aged individuals, the tunica adventitia presents a fiber elastic constitution. The young and aged individuals' parietal venous valve is elastic fiber in nature. The young individuals' ostial valve is also elastic and that of aged individuals is muscular fiber in nature.
Assuntos
Humanos , Masculino , Adolescente , Adulto , Pessoa de Meia-Idade , Veia Femoral/ultraestrutura , Veia Safena/ultraestrutura , Túnica Média/ultraestrutura , Idoso de 80 Anos ou mais , Cadáver , Fibras Musculares Esqueléticas/ultraestrutura , Coxa da Perna/irrigação sanguíneaRESUMO
Injuries of endothelial and smooth muscle cells of autologous vein due to preservation in standard storage media may be responsible for graft failure. The effects of vein preservation with University of Wisconsin solution (UWs) on endothelial and smooth muscle cell function and morphology were compared to the effects of preservation with autologous whole blood (AWB) and normal saline (NS), which are frequently used in cardiovascular surgery. Canine external jugular and common femoral vein segments were preserved in the different solutions at 4 degrees C for 45 min and 24 hr. Rings (4-5 mm in length) from control and preserved veins were evaluated by isometric tension studies at 37 degrees C and by scanning and transmission electron microscopy. Differences between groups were evaluated by Student's t test or Mann-Whitney U test and by analysis of the variance, and considered to be significant at P < 0.05. Sensitivities to norepinephrine (NE) showed that a 45-min vein storage in AWB (5.7 +/- 0.2 mumol/L) but not in NS (5.8 +/- 0.2 mumol/L) or UWs (6.5 +/- 0.2 mumol/L) had a deleterious effect on function of smooth muscle (P < 0.05) when compared to control veins (6.6 +/- 0.2 mumol/L). Maximum contractile responses and sensitivities to NE were significantly altered (P < 0.05) after 24-hr vein storage in AWB (0.09 +/- 0.02 g/mm2 and 5.4 +/- 0.07 mumol/L) and NS (0.12 +/- 0.03 g/mm2 and 5.6 +/- 0.08 mumol/L) but not in UWs (0.36 +/- 0.06 g/mm2 and 6.4 +/- 0.07 mumol/L). With both storage times, acetylcholine-induced endothelium-dependent maximum relaxations and sensitivities were significantly reduced (P < 0.05) in veins stored in AWB and NS, but not in UWs, compared with controls. Similarly, transmission electron microscopy revealed marked neutrophil migration beneath the intimal surface of vessels and extensive separation and desquamation of endothelial cells with exposure of subendothelial structures in veins stored in AWB and NS. The results suggest that UWs is a suitable storage medium when compared to AWB and NS.
Assuntos
Veia Femoral/fisiologia , Veias Jugulares/fisiologia , Preservação de Tecido/métodos , Animais , Cães , Endotélio Vascular/fisiologia , Feminino , Veia Femoral/ultraestrutura , Veias Jugulares/ultraestrutura , Masculino , Microscopia Eletrônica , Microscopia Eletrônica de Varredura , Contração Muscular/efeitos dos fármacos , Relaxamento Muscular/fisiologia , Músculo Liso Vascular/fisiologia , Norepinefrina/farmacologiaRESUMO
Visando a contribuir para o desenvolvimento de métodos de prevençäo de trombose em enxertos veno-venosos realizados por técnica microcirúrgica, o presente trabalho teve por objetivo avaliar comparativamente o efeito da heparina, de uma heparina de baixo peso molecular (enoxaparina) e do sulfato de heparan na prevençäo do desenvolvimento de trombo nesses enxertos no rato, utilizando doses antitrombóticas determinadas em modelo de trombose de veia cava. Pretendeu-se também realizar estudo ultra-estrutural dos enxertos em que se pode, eventualmente, prevenir a formaçäo de trombos, visando verificar uma eventual diferença na açäo das drogas sobre a formaçäo dos mesmos e sobre o endotélio. No modelo de ligadura de veia cava, a heparina teve eficácia nas doses de 200 UI/kg EV e 400 UI/kg SC, a enoxaparina nas doses de 1,5 mg/kg EV e 2 mg/kg SC e o sulfato de heparan nas doses de 2,5 mg/kg EV e 15 mg/kg SC. Os animais foram divididos em 4 grupos de tratamento: grupo controle; grupo heparina, grupo enoxaparina e grupo sulfato de heparan. A heparina foi administrada por via intravenosa, em dose de 200 UI por kg, imediatamente antes do início do procedimento cirúrgico e, após 3 horas, complementada com doses de 400 UI por kg, por via subcutânea, de 12 em 12 horas. A enoxaparina foi administrada por via intravenosa, em dose de 1,5 mg por kg, imediatamente antes do início do procedimento cirúrgico e, após 3 horas, complementada com doses de 2,0 mg por kg, por via subcutânea, de 12 em 12 horas. O sulfato de heparan foi administrado por via intravenosa, em dose de 2,5 mg por kg, imediatamente antes do início do procedimento cirúrgico e, após 3 horas, complementada com doses de 15,0 mg por kg, por via subcutânea, de 12 em 12 horas. O enxerto veno-venoso foi realizado em 56 animais. A avaliaçäo desses enxertos foi realizada 15 minutos (16 ratos) e 48 horas (40 ratos) após a liberaçäo do fluxo sangüíneo. No grupo controle, verificou-se trombose oclusiva do enxerto venoso em 9 dos 10 animais. No grupo tratado com heparina, houve trombose oclusiva em 4 dos 10 animais. No grupo enoxaparina, a trombose oclusiva ocorreu em 9 dos 10, e no grupo sulfato de heparan, em 3 dos animais. Houve diferença significante quanto ao número de trombose oclusiva nos enxertos quando se comparam os animais dos grupos heparina e sulfato de heparan com os animais do grupo controle...
Assuntos
Animais , Masculino , Ratos , Anastomose Cirúrgica , Glicosaminoglicanos/uso terapêutico , Microcirurgia , Trombose/tratamento farmacológico , Trombose/prevenção & controle , Veias/cirurgia , Prótese Vascular , Coagulação Sanguínea , Relação Dose-Resposta a Droga , Endotélio Vascular/ultraestrutura , Veia Femoral/cirurgia , Veia Femoral/transplante , Veia Femoral/ultraestrutura , Heparina de Baixo Peso Molecular/farmacologia , Heparina de Baixo Peso Molecular/uso terapêutico , Heparina/farmacologia , Heparina/uso terapêutico , LigaduraRESUMO
In order to evaluate the normal physiology of the maternal venous circulation, Doppler examinations of the venous system in the right inferior extremity and suprainguinal part of the common femoral vein were carried out in 38 healthy pregnant women, in 12 non-pregnant controls and in 16 women at early puerperium. Examinations were carried out when the mother was lying in a left semi-recumbent supine position. Blood flow velocities in the suprainguinal femoral and deep femoral veins decreased significantly in the first trimester and were almost normalized by the third postpartum day. Continuous forward venous flow with respiratory fluctuation was maintained in the femoral veins over the whole gestational period. The response to the Valsalva maneuver was similar in the pregnant and non-pregnant women. Our findings provide evidence that the venous valvular system and wall distensibility in the femoral area and the inferior extremity are in vivo not greatly changed in pregnancy. A more important reason for decreased velocities is probably the pregnancy-associated decrease of the arterial input to this area.
Assuntos
Perna (Membro)/irrigação sanguínea , Ultrassonografia Doppler em Cores/métodos , Manobra de Valsalva/fisiologia , Adulto , Circulação Sanguínea/fisiologia , Velocidade do Fluxo Sanguíneo/fisiologia , Feminino , Veia Femoral/fisiologia , Veia Femoral/ultraestrutura , Idade Gestacional , Humanos , Gravidez , Terceiro Trimestre da Gravidez , Descanso/fisiologia , Decúbito Dorsal , Ultrassonografia Pré-NatalRESUMO
Glycerol, injected into a site between the femoral vessels of the rat, induced neovascularization, both from the preexisting microcirculation and from the side of the femoral vein facing the artery-vein interstitium where the glycerol was administered. The use of glycerol together with a known angiogenic substance (PGE2) did not modify the neocapillary density (NCD) obtained with glycerol alone. In contrast, the lower level of NCD achieved with an acylglycerol (triacetylglycerol) was increased when the latter was associated with PGE2. Values reached were similar to, but never higher than, those for glycerol alone, or combined with PGE2. The results suggest that glycerol and some substances containing glycerol, amongst which 1-butyrylglycerol has been previously considered, may stimulate angiogenesis by a direct or indirect mechanism of action.
Assuntos
Veia Femoral/fisiologia , Glicerol/farmacologia , Neovascularização Fisiológica/efeitos dos fármacos , Animais , Veia Femoral/ultraestrutura , Microscopia Eletrônica , Músculo Liso Vascular/fisiologia , Músculo Liso Vascular/ultraestrutura , Ratos , Ratos Sprague-DawleyRESUMO
Thirty PTFE prostheses (internal diameter, 1.0 mm; length, 5.0 mm; fibril length, 30 microns) were implanted into rats' femoral veins by means of a coupling device (3M precise microvascular anastomosis system, 3M, St Paul, MN) and evaluated with electron microscopy at regular intervals from 1 day to 3 weeks after implantation to study, in detail, the healing process. Eighty-three percent of mechanically anastomosed grafts were found to be patent. At 1 and 3 days after implantation, the whole length of PTFE was covered with a clot layer containing platelets and a fibrin network. After 1 week, endothelial-like cells originating from the anastomotic sides grew in across the anastomoses. At 3 weeks, the prostheses were completely covered by an endothelial-like cell layer. These results demonstrate that the degree of neo-endothelialization in the microvenous PTFE prosthesis anastomosed with 3M rings was not delayed, as was seen in the healing of microarterial PTFE tubing that was mechanically anastomosed.
Assuntos
Prótese Vascular , Veia Femoral/cirurgia , Politetrafluoretileno , Animais , Veia Femoral/ultraestrutura , Masculino , Microscopia Eletrônica , Microcirurgia , Ratos , Ratos Wistar , Grau de Desobstrução Vascular , CicatrizaçãoRESUMO
Nitric oxide concentration in the periendothelial are of the femoral vein in anaesthetized dogs was measured directly with a catheter- protected porphyrinic sensor. A 2- to 4-fold increase occurred in the basal NO concentration of 90 +/- 12 nM after acetylcholine injection (1-1.5 micrograms/kg). A linear correlation was found between femoral artery blood flow and NO concentration in the periendothelial area of the femoral vein. Noradrenaline decreased NO levels below the detection limit of the porphyrinic sensor (10 nM).
Assuntos
Endotélio Vascular/química , Veia Femoral/metabolismo , Microeletrodos , Óxido Nítrico/sangue , Porfirinas/química , Acetilcolina/farmacologia , Anestesia , Animais , Velocidade do Fluxo Sanguíneo , Pressão Sanguínea/efeitos dos fármacos , Cães , Endotélio Vascular/metabolismo , Endotélio Vascular/ultraestrutura , Feminino , Artéria Femoral/fisiologia , Veia Femoral/ultraestrutura , Glutaral , Masculino , Microscopia Eletrônica , Norepinefrina/farmacologia , Fixação de Tecidos , Vasoconstritores/farmacologiaRESUMO
To date, no arterial substitute has been shown to be as effective as the autologous saphenous vein in peripheral revascularization procedures. In the present study, the venous allograft was evaluated as a vascular substitute in terms of patency and induction of host immune reactivity, whether used in major histocompatibility complex-incompatible, major histocompatibility complex-incompatible dogs. The immunosuppressive drug therapies were given for a period of 31 days, beginning 1 day before transplantation, and consisted of the use of cyclosporine A, mycophenolate mofetil, or a combination of both. All histoincompatible allografts were thrombosed at 4 or 8 weeks after transplantation with antibody development and cell-mediated cytotoxicity in the graft, whereas histocompatible allografts showed late stenosis without immunologic reactions directed toward donor cells. Given alone, neither cyclosporine A nor mycophenolate mofetil improved the overall patency of venous allografts; thrombosis occurred shortly after cessation of immunosuppression. Still, the cyclosporine A-mycophenolate mofetil combination therapy led to a 100% patency rate at 20 weeks after implantation and immune reactions were markedly reduced. This study shows that the fresh vein allograft is still an attractive and functional alternative to the autologous saphenous vein if the host immunologic reactions are controlled by cyclosporine A-mycophenolate mofetil immunosuppression.