RESUMO
Pure phospholipids and membrane fragments from bacterial cells living under various conditions were studied against the influence of the surrounding acidity on the internal dynamics. For that we compared mean square displacements extracted from elastic incoherent neutron scattering data, measured both at low and at neutral pH, of the phospholipids 1,2-dimyristoyl-sn-glycero-3-phosphocholine and of samples from neutralophilic and acidophilic micro-organisms (some being hyperthermophilic and others mesophilic). The lipids showed a slight shift in the phase transition temperature of about 4 degrees under pH variation and became slightly more mobile at lower pH. The membrane fragments not used to extreme acidic conditions were significantly more sensitive to variations in the pH values, whereas the acidophilic and -tolerant samples were much less influenced by this parameter. They presented the higher softness at low pH, which was closer to their native condition. Such findings might be a hint for adaptation mechanisms to different acidity conditions.
Assuntos
Membrana Celular/química , Simulação de Dinâmica Molecular , Acidithiobacillus/química , Acidithiobacillus/fisiologia , Elasticidade , Escherichia coli/química , Escherichia coli/fisiologia , Concentração de Íons de Hidrogênio , Fosfolipídeos/química , Wolinella/química , Wolinella/fisiologiaRESUMO
The active and stable mutant forms of short chain cytoplasmic L-asparaginase type I of Rhodospirillum rubrum (RrA): RrA+N17, D60K, F61L, RrA+N17, A64V, E67K, RrA+N17, E149R, V150P, RrAE149R, V150P and RrAE149R, V150P, F151T were obtained by the method of site-directed mutagenesis. It is established that variants RrA-N17, E149R, V150P, F151T and RrÐE149R, V150P are capable to reduce an expression hTERT subunit of telomerase and, hence, activity of telomeres in Jurkat cells, but not in cellular lysates. During too time, L-asparaginases of Escherichia coli, Erwinia carotovora and Wolinella succinogenes, mutant forms RrÐ+N17, D60K, F61L and RrÐ+N17, A64V, E67K do not suppress of telomerase activity. The assumption of existence in structure RrA of areas (amino acids residues in the position 146-164, 1-17, 60-67) which are responsible for suppression of telomerase activity is made. The received results show that antineoplastic activity of some variants RrA is connected both with reduction of concentration of free L-asparagine, and with expression suppression of hTERT telomerase subunit, that opens new prospects for antineoplastic therapy.
Assuntos
Antineoplásicos/farmacologia , Asparaginase/farmacologia , Proteínas de Bactérias/farmacologia , Mutação Puntual , Rhodospirillum rubrum/enzimologia , Telomerase/antagonistas & inibidores , Telômero/efeitos dos fármacos , Sequência de Aminoácidos , Antineoplásicos/química , Antineoplásicos/metabolismo , Asparaginase/química , Asparaginase/genética , Asparaginase/metabolismo , Proteínas de Bactérias/química , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Escherichia coli/química , Escherichia coli/enzimologia , Escherichia coli/genética , Expressão Gênica , Células HL-60 , Humanos , Células Jurkat , Modelos Moleculares , Mutagênese Sítio-Dirigida , Pectobacterium carotovorum/química , Pectobacterium carotovorum/enzimologia , Pectobacterium carotovorum/genética , Plasmídeos/química , Plasmídeos/metabolismo , Estrutura Secundária de Proteína , Rhodospirillum rubrum/química , Rhodospirillum rubrum/genética , Especificidade da Espécie , Relação Estrutura-Atividade , Telomerase/genética , Telomerase/metabolismo , Telômero/química , Wolinella/química , Wolinella/enzimologia , Wolinella/genéticaRESUMO
The diheme cytochromes c of the widespread TsdA family are bifunctional thiosulfate dehydrogenase/tetrathionate reductases. Here, biochemical information was collected about TsdA from the Epsilonproteobacterium Wolinella succinogenes (WsTsdA). The situation in W. succinogenes is unique since TsdA is closely associated with the unprecedented lipoprotein TsdC encoded immediately downstream of tsdA in the same direction of transcription. WsTsdA purified from Escherichia coli catalyzed both thiosulfate oxidation and tetrathionate reduction. After co-production of TsdC and WsTsdA in E. coli, TsdC was found to mediate membrane attachment of TsdA and to ensure its full catalytic activity. This effect was much stronger in the tetrathionate-reducing than in the thiosulfate-oxidizing direction. It is concluded that the TsdAC complex predominantly acts as a tetrathionate reductase in vivo.
Assuntos
Proteínas de Bactérias/metabolismo , Lipoproteínas/metabolismo , Oxirredutases/metabolismo , Wolinella/química , Wolinella/enzimologia , Biocatálise , Escherichia coli/metabolismo , Lipoproteínas/isolamento & purificação , Oxirredução , Wolinella/metabolismoRESUMO
The α-helical transmembrane proteins constitute 25% of the entire human proteome space and are difficult targets in high-resolution wet-lab structural studies, calling for accurate computational predictors. We present a novel sequence-based method called MemBrain-Rasa to predict relative solvent accessibility surface area (rASA) from primary sequences. MemBrain-Rasa features by an ensemble prediction protocol composed of a statistical machine-learning engine, which is trained in the sequential feature space, and a segment template similarity-based engine, which is constructed with solved structures and sequence alignment. We locally constructed a comprehensive database of residue relative solvent accessibility surface area from the solved protein 3D structures in the PDB database. It is searched against for segment templates that are expected to be structurally similar to the query sequence's segments. The segment template-based prediction is then fused with the support vector regression outputs using knowledge rules. Our experiments show that pure machine learning output cannot cover the entire rASA solution space and will have a serious prediction preference problem due to the relatively small size of membrane protein structures that can be used as the training samples. The template-based engine solves this problem very well, resulting in significant improvement of the prediction performance. MemBrain-Rasa achieves a Pearson correlation coefficient of 0.733 and mean absolute error of 13.593 on the benchmark dataset, which are 26.4% and 26.1% better than existing predictors. MemBrain-Rasa represents a new progress in structure modeling of α-helical transmembrane proteins. MemBrain-Rasa is available at www.csbio.sjtu.edu.cn/bioinf/MemBrain/.
Assuntos
Aprendizado de Máquina , Proteínas de Membrana/química , Modelos Químicos , Sequência de Aminoácidos , Animais , Proteínas de Bactérias/química , Bases de Dados de Proteínas , Humanos , Dados de Sequência Molecular , Estrutura Quaternária de Proteína , Estrutura Secundária de Proteína , Alinhamento de Sequência , Solubilidade , Solventes/química , Succinato Desidrogenase/química , Wolinella/químicaRESUMO
AIM: Evaluate immune response in mice against various L-asparaginases and determine their cross-immunogenicity. MATERIALS AND METHODS: The studies were carried out in C57Bl(6j) line mice. Immunogenicity of L-asparaginases was studied: Escherichia coli type II (recombinant) (Medak, Germany) (EcA); Erwinia carotovora type II (ErA); Yersinia pseudotuberculosis type II (YpA); Rhodospirillum rubrum type I (RrA); Wollinella succinogenes type II (WsA). Immune response against the administered antigens was determined in EIA. RESULTS: Y. pseudotuberculosis L-asparaginase was the most immunogenic, E. coli--the least immunogenic. E. carotovora, R. rubrum, W. succinogenes asparaginases displayed intermediate immunogenicity. The results of cross-immunogenicity evaluation have established, that blood sera of mice, that had received YpA, showed cross-immunogenicity against all the other L-asparaginase preparations except E. carotovora. During immunization with E. coli L-asparaginase the developed antibodies also bound preparation from E. carotovora. Sera from mice immunized with W. succinogenes, E. carotovora and R. rubrum L-asparaginases had cross-reaction only with EcA and did not react with other preparations. CONCLUSION: Cross-immunogenicity of the studied L-asparaginases was determined. A sequence of administration of the studied preparation is proposed that allows to minimize L-asparaginase neutralization by cross-reacting antibodies.
Assuntos
Anticorpos Antibacterianos/sangue , Antígenos de Bactérias/imunologia , Asparaginase/imunologia , Proteínas de Bactérias/imunologia , Animais , Especificidade de Anticorpos , Antígenos de Bactérias/administração & dosagem , Antígenos de Bactérias/isolamento & purificação , Asparaginase/administração & dosagem , Asparaginase/isolamento & purificação , Proteínas de Bactérias/administração & dosagem , Proteínas de Bactérias/isolamento & purificação , Reações Cruzadas , Escherichia coli/química , Escherichia coli/enzimologia , Soros Imunes , Camundongos , Camundongos Endogâmicos C57BL , Pectobacterium carotovorum/química , Pectobacterium carotovorum/enzimologia , Rhodospirillum rubrum/química , Rhodospirillum rubrum/enzimologia , Wolinella/química , Wolinella/enzimologia , Yersinia pseudotuberculosis/química , Yersinia pseudotuberculosis/enzimologiaRESUMO
In this article, we consider, in detail, the second half-cycle of the six-electron nitrite reduction mechanism catalyzed by cytochrome c nitrite reductase. In total, three electrons and four protons must be provided to reach the final product, ammonia, starting from the HNO intermediate. According to our results, the first event in this half-cycle is the reduction of the HNO intermediate, which is accomplished by two PCET reactions. Two isomeric radical intermediates, HNOH(â¢) and H2NO(â¢), are formed. Both intermediates are readily transformed into hydroxylamine, most likely through intramolecular proton transfer from either Arg114 or His277. An extra proton must enter the active site of the enzyme to initiate heterolytic cleavage of the N-O bond. As a result of N-O bond cleavage, the H2N(+) intermediate is formed. The latter readily picks up an electron, forming H2N(+â¢), which in turn reacts with Tyr218. Interestingly, evidence for Tyr218 activity was provided by the mutational studies of Lukat (Biochemistry 47:2080, 2008), but this has never been observed in the initial stages of the overall reduction process. According to our results, an intramolecular reaction with Tyr218 in the final step of the nitrite reduction process leads directly to the final product, ammonia. Dissociation of the final product proceeds concomitantly with a change in spin state, which was also observed in the resonance Raman investigations of Martins et al. (J Phys Chem B 114:5563, 2010).
Assuntos
Amônia/metabolismo , Citocromos a1/metabolismo , Citocromos c1/metabolismo , Heme/metabolismo , Hidroxilamina/metabolismo , Nitrato Redutases/metabolismo , Óxidos de Nitrogênio/metabolismo , Wolinella/enzimologia , Amônia/química , Citocromos a1/química , Citocromos c1/química , Heme/química , Hidroxilamina/química , Ligantes , Modelos Moleculares , Nitrato Redutases/química , Óxidos de Nitrogênio/química , Wolinella/química , Wolinella/metabolismoRESUMO
Various models on membrane structure and organization of proteins and complexes in natural membranes emerged during the last years. However, the lack of systematic dynamical studies to complement structural investigations hindered the establishment of a more complete picture of these systems. Elastic incoherent neutron scattering gives access to the dynamics on a molecular level and was applied to natural membranes extracted from the hyperthermophile Aquifex aeolicus and the mesophile Wolinella succinogenes bacteria. The results permitted to extract a hierarchy of dynamic flexibility and atomic resilience within the samples, which correlated with the organization of proteins in bioenergetics complexes and the functionality of the membranes.
Assuntos
Membrana Celular/química , Bactérias Gram-Negativas/química , Difração de Nêutrons , Wolinella/química , Metabolismo Energético , Movimento (Física) , TermodinâmicaRESUMO
Cytochrome c nitrite reductase catalyzes the six-electron, seven-proton reduction of nitrite to ammonia without release of any detectable reaction intermediate. This implies a unique flexibility of the active site combined with a finely tuned proton and electron delivery system. In the present work, we employed density functional theory to study the recharging of the active site with protons and electrons through the series of reaction intermediates based on nitrogen monoxide [Fe(II)-NO(+), Fe(II)-NO·, Fe(II)-NO(-), and Fe(II)-HNO]. The activation barriers for the various proton and electron transfer steps were estimated in the framework of Marcus theory. Using the barriers obtained, we simulated the kinetics of the reduction process. We found that the complex recharging process can be accomplished in two possible ways: either through two consecutive proton-coupled electron transfers (PCETs) or in the form of three consecutive elementary steps involving reduction, PCET, and protonation. Kinetic simulations revealed the recharging through two PCETs to be a means of overcoming the predicted deep energetic minimum that is calculated to occur at the stage of the Fe(II)-NO· intermediate. The radical transfer role for the active-site Tyr(218), as proposed in the literature, cannot be confirmed on the basis of our calculations. The role of the highly conserved calcium located in the direct proximity of the active site in proton delivery has also been studied. It was found to play an important role in the substrate conversion through the facilitation of the proton transfer steps.
Assuntos
Citocromos a1/metabolismo , Citocromos c1/metabolismo , Ferro/metabolismo , Nitrato Redutases/metabolismo , Óxido Nítrico/metabolismo , Óxidos de Nitrogênio/metabolismo , Wolinella/enzimologia , Domínio Catalítico , Citocromos a1/química , Citocromos c1/química , Transporte de Elétrons , Ativação Enzimática , Heme/química , Heme/metabolismo , Modelos Moleculares , Nitrato Redutases/química , Oxirredução , Prótons , Teoria Quântica , Termodinâmica , Wolinella/químicaRESUMO
Bacterial cytochrome c maturation occurs at the outside of the cytoplasmic membrane, requires transport of haem b across the membrane, and depends on membrane-bound cytochrome c haem lyase (CCHL), an enzyme that catalyses covalent attachment of haem b to apocytochrome c. Epsilonproteobacteria such as Wolinella succinogenes use the cytochrome c biogenesis system II and contain unusually large CCHL proteins of about 900 amino acid residues that appear to be fusions of the CcsB and CcsA proteins found in other bacteria. CcsBA-type CCHLs have been proposed to act as haem transporters that contain two haem b coordination sites located at different sides of the membrane and formed by histidine pairs. W. succinogenes cells contain three CcsBA-type CCHL isoenzymes (NrfI, CcsA1 and CcsA2) that are known to differ in their specificity for apocytochromes and apparently recognize different haem c binding motifs such as CX(2)CH (by CcsA2), CX(2)CK (by NrfI) and CX(15)CH (by CcsA1). In this study, conserved histidine residues were individually replaced by alanine in each of the W. succinogenes CCHLs. Characterization of NrfI and CcsA1 variants in W. succinogenes demonstrated that a set of four histidines is essential for maturing the dedicated multihaem cytochromes c NrfA and MccA, respectively. The function of W. succinogenes CcsA2 variants produced in Escherichia coli was also found to depend on each of these four conserved histidine residues. The presence of imidazole in the growth medium of both W. succinogenes and E. coli rescued the cytochrome c biogenesis activity of most histidine variants, albeit to different extents, thereby implying the presence of two functionally distinct histidine pairs in each CCHL. The data support a model in which two conserved haem b binding sites are involved in haem transport catalysed by CcsBA-type CCHLs.
Assuntos
Proteínas de Bactérias/química , Heme/metabolismo , Histidina/metabolismo , Liases/química , Wolinella/enzimologia , Motivos de Aminoácidos , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Sítios de Ligação , Sequência Conservada , Histidina/química , Histidina/genética , Liases/genética , Liases/metabolismo , Wolinella/química , Wolinella/genéticaRESUMO
Various nitrate-reducing bacteria produce proteins of the periplasmic nitrate reductase (Nap) system to catalyse electron transport from the membraneous quinol pool to the periplasmic nitrate reductase NapA. The composition of the corresponding nap gene clusters varies but, in addition to napA, genes encoding at least one membrane-bound quinol dehydrogenase module (NapC and/or NapGH) are regularly present. Moreover, some nap loci predict accessory proteins such as the iron-sulfur protein NapF, whose function is poorly understood. Here, the role of NapF in nitrate respiration of the Epsilonproteobacterium Wolinella succinogenes was examined. Immunoblot analysis showed that NapF is located in the membrane fraction in nitrate-grown wild-type cells whereas it was found to be a soluble cytoplasmic protein in a napH deletion mutant. This finding indicates the formation of a membrane-bound NapGHF complex that is likely to catalyse NapH-dependent menaquinol oxidation and electron transport to the iron-sulfur adaptor proteins NapG and NapF, which are located on the periplasmic and cytoplasmic side of the membrane, respectively. The cysteine residues of a CX(3)CP motif and of the C-terminal tetra-cysteine cluster of NapH were found to be required for interaction with NapF. A napF deletion mutant accumulated the catalytically inactive cytoplasmic NapA precursor, suggesting that electron flow or direct interaction between NapF and NapA facilitated NapA assembly and/or export. On the other hand, NapA maturation and activity was not impaired in the absence of NapH, demonstrating that soluble NapF is functional. Each of the four tetra-cysteine motifs of NapF was modified but only one motif was found to be essential for efficient NapA maturation. It is concluded that the NapGHF complex plays a multifunctional role in menaquinol oxidation, electron transfer to periplasmic NapA and maturation of the cytoplasmic NapA precursor.
Assuntos
Nitrato Redutases/metabolismo , Nitratos/metabolismo , Periplasma/metabolismo , Wolinella/enzimologia , Motivos de Aminoácidos , Proteínas da Membrana Bacteriana Externa/química , Proteínas da Membrana Bacteriana Externa/genética , Proteínas da Membrana Bacteriana Externa/metabolismo , Citoplasma/metabolismo , Família Multigênica , Complexos Multiproteicos , Nitrato Redutases/química , Nitrato Redutases/genética , Nitratos/química , Oxirredução , Wolinella/química , Wolinella/genéticaRESUMO
Nitrate respiration catalysed by the epsilon-proteobacterium Wolinella succinogenes relies on the NapAGHBFLD system that comprises periplasmic nitrate reductase (NapA) and various other Nap proteins required for electron transport from menaquinol to NapA or maturation of Nap components. The W. succinogenes Nap system is unusual as electron transfer to NapA was shown previously to depend on both subunits of the predicted menaquinol dehydrogenase complex NapGH but did not require a cytochrome c of the NapC/NrfH family. Nonetheless, minor residual growth by nitrate respiration was observed in napG and napH gene inactivation mutants. Here, the question is addressed whether alternative membrane-bound menaquinol dehydrogenases, like NrfH and NosGH, involved in nitrite or N2O reduction systems, are able to functionally replace NapGH. The phenotypes of various gene deletion mutants as well as strains expressing chimeric nap/nos operons demonstrate that NosH is able to donate electrons to the respiratory chain of nitrate respiration at a physiologically relevant rate, whereas NrfH and NosG are not. The iron-sulphur protein NapG was shown to form a complex with NapH in the membrane but was detected in the periplasmic cell fraction in the absence of NapH. Likewise, NosH is able to bind NapG. Each of the eight poly-cysteine motifs present in either NapG or NapH was shown to be essential for nitrate respiration. The NapG homologue NosG could not substitute for NapG, even after adjusting the cysteine spacing to that of NapG, implying that NapG and NosG are specific adapter proteins that channel electrons into either the Nap or Nos system. The current model on the structure and function of the NapGH menaquinol dehydrogenase complex is presented and the composition of the electron transport chains that deliver electrons to periplasmic reductases for either nitrate, nitrite or N2O is discussed.
Assuntos
Proteínas de Bactérias/metabolismo , Nitrato Redutase/metabolismo , Nitratos/metabolismo , Wolinella/enzimologia , Motivos de Aminoácidos , Proteínas de Bactérias/química , Proteínas de Bactérias/genética , Membrana Celular/química , Membrana Celular/enzimologia , Membrana Celular/genética , Membrana Celular/metabolismo , Transporte de Elétrons , Expressão Gênica , Genoma Bacteriano , Nitrato Redutase/química , Nitrato Redutase/genética , Óperon , Wolinella/química , Wolinella/genética , Wolinella/metabolismoRESUMO
Membrane protein complexes can support both the generation and utilisation of a transmembrane electrochemical proton potential ('proton-motive force'), either by transmembrane electron transfer coupled to protolytic reactions on opposite sides of the membrane or by transmembrane proton transfer. Here we provide the first evidence that both of these mechanisms are combined in the case of a specific respiratory membrane protein complex, the dihaem-containing quinol:fumarate reductase (QFR) of Wolinella succinogenes, so as to facilitate transmembrane electron transfer by transmembrane proton transfer. We also demonstrate the non-functionality of this novel transmembrane proton transfer pathway ('E-pathway') in a variant QFR where a key glutamate residue has been replaced. The 'E-pathway', discussed on the basis of the 1.78-Angstrom-resolution crystal structure of QFR, can be concluded to be essential also for the viability of pathogenic epsilon-proteobacteria such as Helicobacter pylori and is possibly relevant to proton transfer in other dihaem-containing membrane proteins, performing very different physiological functions.
Assuntos
Proteínas de Bactérias/química , Proteínas de Membrana/química , Oxirredutases/química , Prótons , Wolinella/química , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Transporte Biológico/genética , Cristalografia por Raios X/métodos , Transporte de Elétrons/genética , Helicobacter pylori/química , Helicobacter pylori/genética , Helicobacter pylori/metabolismo , Proteínas de Membrana/genética , Proteínas de Membrana/metabolismo , Oxirredutases/genética , Oxirredutases/metabolismo , Estrutura Terciária de Proteína , Bombas de Próton/química , Bombas de Próton/genética , Bombas de Próton/metabolismo , Especificidade da Espécie , Wolinella/genética , Wolinella/metabolismoRESUMO
The quinol:fumarate reductase of Wolinella succinogenes binds a low- and a high-potential heme b group in its transmembrane subunit C. Both hemes are part of the electron transport chain between the two catalytic sites of this redox enzyme. The oxidation-reduction midpoint potentials of the hemes are well established but their assignment in the structure has not yet been determined. By simulating redox titrations, using continuum electrostatics calculations, it was possible to achieve an unequivocal assignment of the low- and high-potential hemes to the distal and proximal positions in the structure, respectively. Prominent features governing the differences in midpoint potential between the two hemes are the higher loss of reaction field energy for the proximal heme and the stronger destabilization of the oxidized form of the proximal heme due to several buried Arg and Lys residues. According to the so-called "E-pathway hypothesis", quinol:fumarate reductase has previously been postulated to exhibit a novel coupling of transmembrane electron and proton transfer. Simulation of heme b reduction indicates that the protonation state of the conserved residue Glu C180, predicted to play a key role in this process, indeed depends on the redox state of the hemes. This result clearly supports the E-pathway hypothesis.
Assuntos
Membrana Celular/química , Membrana Celular/metabolismo , Modelos Biológicos , Modelos Químicos , Oxirredutases/química , Oxirredutases/metabolismo , Wolinella/química , Wolinella/metabolismo , Simulação por Computador , Transporte de Elétrons , Ativação Enzimática , Heme/química , Heme/metabolismo , Oxirredução , Prótons , Eletricidade Estática , Relação Estrutura-AtividadeRESUMO
The majority of bacterial membrane-bound NiFe-hydrogenases and formate dehydrogenases have homologous membrane-integral cytochrome b subunits. The prototypic NiFe-hydrogenase of Wolinella succinogenes (HydABC complex) catalyzes H2 oxidation by menaquinone during anaerobic respiration and contains a membrane-integral cytochrome b subunit (HydC) that carries the menaquinone reduction site. Using the crystal structure of the homologous FdnI subunit of Escherichia coli formate dehydrogenase-N as a model, the HydC protein was modified to examine residues thought to be involved in menaquinone binding. Variant HydABC complexes were produced in W. succinogenes, and several conserved HydC residues were identified that are essential for growth with H2 as electron donor and for quinone reduction by H2. Modification of HydC with a C-terminal Strep-tag II enabled one-step purification of the HydABC complex by Strep-Tactin affinity chromatography. The tagged HydC, separated from HydAB by isoelectric focusing, was shown to contain 1.9 mol of heme b/mol of HydC demonstrating that HydC ligates both heme b groups. The four histidine residues predicted as axial heme b ligands were individually replaced by alanine in Strep-tagged HydC. Replacement of either histidine ligand of the heme b group proximal to HydAB led to HydABC preparations that contained only one heme b group. This remaining heme b could be completely reduced by quinone supporting the view that the menaquinone reduction site is located near the distal heme b group. The results indicate that both heme b groups are involved in electron transport and that the architecture of the menaquinone reduction site near the cytoplasmic side of the membrane is similar to that proposed for E. coli FdnI.
Assuntos
Citocromos b/metabolismo , Hidrogenase/metabolismo , Vitamina K 2/metabolismo , Wolinella/química , Substituição de Aminoácidos , Sequência de Bases , Sítios de Ligação , Citocromos b/química , Primers do DNA , Hidrogenase/química , Hidrogenase/genética , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Oxirredução , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismoRESUMO
A TROSY-based triple-resonance pulse scheme is described which correlates backbone 1H and 15N chemical shifts of an amino acid residue with the 15N chemical shifts of both the sequentially preceding and following residues. The sequence employs 1J(NC alpha) and 2J(NC alpha) couplings in two sequential magnetization transfer steps in an 'out-and-back' manner. As a result, N,N connectivities are obtained irrespective of whether the neighbouring amide nitrogens are protonated or not, which makes the experiment suitable for the assignment of proline resonances. Two different three-dimensional variants of the pulse sequence are presented which differ in sensitivity and resolution to be achieved in one of the nitrogen dimensions. The new method is demonstrated with two uniformly 2H/13C/15N-labelled proteins in the 30-kDa range.
Assuntos
Ressonância Magnética Nuclear Biomolecular/métodos , Prolina/química , Proteínas/química , Sequência de Aminoácidos , Grupo dos Citocromos c/química , Grupo dos Citocromos c/metabolismo , Deutério/metabolismo , Magnetismo , Complexos Multienzimáticos/química , Complexos Multienzimáticos/metabolismo , Isótopos de Nitrogênio , Oxirredutases/química , Oxirredutases/metabolismo , Proteínas/metabolismo , Sensibilidade e Especificidade , Sulfurtransferases/química , Sulfurtransferases/metabolismo , Wolinella/químicaRESUMO
Wolinella succinogenes can grow by anaerobic respiration with fumarate or polysulfide as the terminal electron acceptor, and H2 or formate as the electron donor. A DeltahydABC mutant lacking the hydrogenase structural genes did not grow with H2 and either fumarate or polysulfide. In contrast to the wild-type strain, the mutant grown with fumarate and with formate instead of H2 did not catalyze the reduction of fumarate, polysulfide, dimethylnaphthoquinone, or benzyl viologen by H2. Growth and enzymic activities were restored upon integration of a plasmid carrying hydABC into the genome of the DeltahydABC mutant. The DeltahydABC mutant was complemented with hydABC operons modified by artificial stop codons in hydA (StopA) or at the 5'-end of hydC (StopC). The StopC mutant lacked HydC, and the hydrophobic C-terminus of HydA was missing in the hydrogenase of the StopA mutant. The two mutants catalyzed benzyl viologen reduction by H2. The enzyme activity was located in the membrane of the mutants. A mutant with both modifications (StopAC) contained the activity in the periplasm. The three mutants did not grow with H2 and either fumarate or polysulfide, and did not catalyze dimethylnaphthoquinone reduction by H2. We conclude that the same hydrogenase serves in the anaerobic respiration with fumarate and with polysulfide. HydC and the C-terminus of HydA appear to be required for both routes of electron transport and for dimethylnaphthoquinone reduction by H2. The hydrogenase is anchored in the membrane by HydC and by the C-terminus of HydA. The catalytic subunit HydB is oriented towards the periplasmic side of the membrane.
Assuntos
Hidrogenase/metabolismo , Wolinella/química , Sequência de Aminoácidos , Animais , Benzil Viologênio/metabolismo , Clonagem Molecular , Escherichia coli/genética , Formiatos/metabolismo , Fumaratos/metabolismo , Genes Bacterianos/genética , Hidrogênio/metabolismo , Hidrogenase/genética , Dados de Sequência Molecular , Poliéster Sulfúrico de Pentosana/metabolismo , Coelhos , Alinhamento de Sequência , Wolinella/enzimologia , Wolinella/genética , Wolinella/crescimento & desenvolvimentoRESUMO
Lipopolysaccharides (LPSs) of 11 bacterial strains from the type species of the genera Bacteroides (B. fragilis), Prevotella (Pr. melaninogenica), Porphyromonas (Po. gingivalis), Campylobacter (C. fetus subsp. fetus), and Wolinella (W. succinogenes), and from the type strains of B. distasonis, B. forsythus, B. ureolyticus, Po. levii, Po. macacae, and C. gracilis, were extracted with hot water-phenol (Westphal method). S-form LPSs, obtained from all organisms, were well resolved with tricine-sodium-dodecyl-sulphate polyacrylamide gel electrophoresis and visualized by silver staining. Lipid A was not stained. Also profiles from LPS of Escherichia coli, serotypes 0111:B4 and 055:B5, could be distinguished. While W. succinogenes showed a relatively short S-form LPS on electrophoregrams, the other bacteria, including B. fragilis, exhibited long-ladder LPSs. Po. gingivalis displayed the largest number of bands and the longest O-chain. The long O-chain of this bacterium may be important for its virulence.
Assuntos
Antígenos de Bactérias/química , Lipopolissacarídeos/química , Bacteroides/química , Campylobacter/química , Eletroforese em Gel de Poliacrilamida/métodos , Glicina/análogos & derivados , Bactérias Anaeróbias Gram-Negativas , Peso Molecular , Porphyromonas/química , Prevotella melaninogenica/química , Virulência , Wolinella/químicaRESUMO
A monomeric flavoprotein (18.8 kDa) was isolated from the soluble cell fraction of Wolinella succinogenes and was identified as a flavodoxin based on its N-terminal sequence, FMN content, and redox properties. The midpoint potentials of the flavodoxin (Fld) at pH 7. 5 were measured as -95 mV (Fldox/Flds) and -450 mV (Flds/Fldred) relative to the standard hydrogen electrode. The cellular flavodoxin content [0.3 micromol (g protein)-1] was the same in bacteria grown with fumarate or with polysulfide as the terminal acceptor of electron transport. The flavodoxin did not accept electrons from hydrogenase or formate dehydrogenase, the donor enzymes of electron transport to fumarate or polysulfide. Pyruvate:flavodoxin oxidoreductase activity [180 U (g cellular protein)-1] was detected in the soluble cell fraction of W. succinogenes grown with fumarate or polysulfide. The enzyme was equally active with Fldox or Flds at high concentrations. The Km for Flds (80 microM) was larger than that for Fldox and for the ferredoxin isolated from W. succinogenes (15 microM). We conclude that flavodoxin serves anabolic rather than catabolic functions in W. succinogenes.
Assuntos
Flavodoxina/isolamento & purificação , Wolinella/química , Sequência de Aminoácidos , Flavodoxina/química , Flavodoxina/metabolismo , Cetona Oxirredutases/metabolismo , Dados de Sequência Molecular , Oxirredução , Piruvato SintaseRESUMO
Three major outer membrane proteins with apparent molecular masses of 43, 45, and 51 kDa were purified from Wolinella recta ATCC 33238, and their pore-forming abilities were determined by the black lipid bilayer method. The non-heat-modifiable 45-kDa protein (Omp 45) showed no pore-forming activity even at high KCl concentrations. The single-channel conductances in 1 M KCl of the heat-modifiable proteins with apparent molecular masses of 43 kDa (Omp 43) and 51 kDa (Omp 51) were 0.49 and 0.60 nS, respectively. The proteins formed nonselective channels and, as determined by experiments of ion selectivity and zero-current potential, were weakly anion selective.
Assuntos
Proteínas da Membrana Bacteriana Externa/isolamento & purificação , Canais Iônicos/fisiologia , Wolinella/química , Proteínas da Membrana Bacteriana Externa/imunologia , Cloretos/metabolismo , Peso Molecular , Porinas , Potássio/metabolismo , Wolinella/fisiologiaRESUMO
Lipopolysaccharides (LPS) were extracted from cells of Wolinella recta ATCC 33238, W. curva ATCC 33224, W. succinogenes ATCC 29543 and Campylobacter sputorum ssp. sputorum A 3563 by a hot phenol-water method and purified by nuclease treatment and by repeated ultracentrifugation. Chemical compositions of the purified LPS including fatty acid and sugar composition were examined and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed. All LPS preparations contained a monosaccharide identified as L-glycero-D-mannoheptose, and another heptose isomer identified as D-glycero-D-mannoheptose was a typical constituent of the LPS from all three Wolinella species.