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1.
Acta Biomed ; 92(S1): e2021136, 2021 11 04.
Artigo em Inglês | MEDLINE | ID: mdl-34747374

RESUMO

A fifty-three year-old military patient, presented with a 3 months neck pain and paraesthesia of upper right extremity after gastroscopy. Radiological examination demonstrated anuncertain degenerative/infective involvement of C6-C7 intervertebral disc. The patient underwent anterior debridement with C5-C6 disc excisional biopsy, with microbiological findings of Bacillus pumilus with high bacterial concentration. After biopsy, segment arthrodesis was achieved only with vertebral plate cruentation and 3 months of cervical collar. He completed a six-weeks course of intravenous antibiotics for the treatment of atypical spondylodiscitis. At one year of follow-up, he had no residual neck pain or neurological signs or symptoms.


Assuntos
Bacillus pumilus , Discite , Deslocamento do Disco Intervertebral , Vértebras Cervicais/diagnóstico por imagem , Vértebras Cervicais/cirurgia , Discite/complicações , Discite/diagnóstico por imagem , Discite/tratamento farmacológico , Humanos , Masculino , Pessoa de Meia-Idade , Cervicalgia/etiologia
2.
Molecules ; 26(15)2021 Jul 24.
Artigo em Inglês | MEDLINE | ID: mdl-34361631

RESUMO

The cyclic lipopeptide produced from Bacillus pumilus strain HY1 was isolated from Korean soybean sauce cheonggukjang. The chemical structures of the surfactin isomers were analyzed using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and electrospray ionization tandem mass spectrometry (ESI-MS/MS). The five potential surfactin isoforms were detected with protonated masses of m/z 994.7, 1008.7, 1022.7, 1036.7, and 1050.7 and different structures in combination with Na+, K+, and Ca2+ ions. ESI-MS/MS analysis revealed that the isolated surfactin possessed the precise amino acid sequence LLVDLL and hydroxyl fatty acids with 12 to 16 carbons. The surfactin content during cheonggukjang fermentation increased from 0.3 to 51.2 mg/kg over 60 h of fermentation. The mixture of five surfactin isoforms of cheonggukjang inhibited the growth of two cancer cell lines. The growth of both MCF-7 and Caco-2 cells was strongly inhibited with 100 µg/µL of surfactin. This study is the first-time report of five surfactin isomers of Bacillus pumilus strain HY1 during Korean soybean sauce cheonggukjang fermentation, which has cytotoxic properties.


Assuntos
Bacillus pumilus/metabolismo , Proliferação de Células/efeitos dos fármacos , Alimentos e Bebidas Fermentados/microbiologia , Lipopeptídeos , Soja/microbiologia , Células CACO-2 , Humanos , Lipopeptídeos/química , Lipopeptídeos/isolamento & purificação , Lipopeptídeos/farmacologia , Células MCF-7
3.
Braz J Biol ; 83: e243874, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34378658

RESUMO

In recent days, cheapest alternative carbon source for fermentation purpose is desirable to minimize production cost. Xylanases have become attractive enzymes as their potential in bio-bleaching of pulp and paper industry. The objective of the present study was to identify the potential ability on the xylanase production by locally isolated Bacillus pumilus BS131 by using waste fiber sludge and wheat bran media under submerged fermentation. Culture growth conditions were optimized to obtain significant amount of xylanase. Maximum xylanase production was recorded after 72 hours of incubation at 30 °C and 7 pH with 4.0% substrate concentration. In the nutshell, the production of xylanase using inexpensive waste fiber sludge and wheat-bran as an alternative in place of expensive xylan substrate was more cost effective and environment friendly.


Assuntos
Bacillus pumilus , Bacillus , Bacillus/metabolismo , Bacillus pumilus/metabolismo , Fibras na Dieta , Endo-1,4-beta-Xilanases/metabolismo , Fermentação , Concentração de Íons de Hidrogênio , Esgotos , Temperatura
4.
Int J Mol Sci ; 22(15)2021 Jul 29.
Artigo em Inglês | MEDLINE | ID: mdl-34360927

RESUMO

A 98.1 Kb genomic region from B. pumilus 15.1, a strain isolated as an entomopathogen toward C. capitata, the Mediterranean fruit fly, has been characterised in search of potential virulence factors. The 98.1 Kb region shows a high number of phage-related protein-coding ORFs. Two regions with different phylogenetic origins, one with 28.7 Kb in size, highly conserved in Bacillus strains, and one with 60.2 Kb in size, scarcely found in Bacillus genomes are differentiated. The content of each region is thoroughly characterised using comparative studies. This study demonstrates that these two regions are responsible for the production, after mitomycin induction, of a phage-like particle that packages DNA from the host bacterium and a novel phage for B. pumilus, respectively. Both the phage-like particles and the novel phage are observed and characterised by TEM, and some of their structural proteins are identified by protein fingerprinting. In addition, it is found that the phage-like particle shows bacteriocin activity toward other B. pumilus strains. The effect of the phage-like particles and the phage in the toxicity of the strain toward C. capitata is also evaluated.


Assuntos
Bacillus pumilus , Bacteriocinas/metabolismo , Bacteriófagos , Ceratitis capitata/microbiologia , Fatores de Virulência/metabolismo , Animais , Bacillus pumilus/isolamento & purificação , Bacillus pumilus/patogenicidade , Bacillus pumilus/virologia , Bacteriófagos/patogenicidade , Bacteriófagos/fisiologia
5.
Nuklearmedizin ; 60(6): 445-449, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34416783

RESUMO

BACKGROUND: For radiopharmaceuticals, aseptic preparation in combination with filtration is the most commonly used sterilizing method. In general, the production of radiopharmaceuticals needs to fulfil the requirements of good manufacturing practice. In the scope of this work, we focused on the positron emitter gallium-68 and on the therapeutically used beta- and gamma-emitter lutetium-177, as they are routinely used for in-house synthesis of radiopharmaceuticals in nuclear medicine departments. Our hypothesis is, that radiopharmaceuticals might be self-sterilizing due to a high radioactivity concentration and high-energy radionuclides in the preparation for intravenous injections. RESULTS: Incubation with gallium-68 and lutetium-177 for both 30 minutes and 5 hours post-dispensing did not cause any significant effect on bacteria growth. As the theoretical dose is only 0.1-0.6 % of the Ph. Eur. recommended dose of 25 kGy, we conclude that the beta and positron energy of lutetium-177 and gallium-68 as used for standard radiopharmaceutical in-house production is not sufficient to decrease the number of colony forming units compared to the control values. CONCLUSIONS: Based on these findings, gallium-68 and lutetium-177 labeled radiopharmaceuticals are not self-sterilizing under the tested conditions with respect to bacillus pumilus and staphylococcus succinus. Consequently, strict aseptic preparation conditions in addition to end-sterilization of the radiopharmaceutical e.g. through membrane filtration are strongly advised for in-house productions.


Assuntos
Bacillus pumilus , Compostos Radiofarmacêuticos , Radioisótopos de Gálio , Lutécio , Radioisótopos , Staphylococcus , Esterilização
6.
Sci Rep ; 11(1): 17196, 2021 08 25.
Artigo em Inglês | MEDLINE | ID: mdl-34433897

RESUMO

Heavy metals contaminate the soil that alters the properties of soil and negatively affect plants growth. Using microorganism and plant can remove these pollutants from soil. The present investigation was designed to evaluate the induced effect of Bacillus pumilus on maize plant in Cadmium (Cd) contaminated soil. Three different concentrations of Cd (i.e. 0.25, 0.50 and 0.75 mg kg-1) were applied in soil under which maize plants were grown. The germination percentage, shoot length, leaf length, number of leaves, root length, fresh weight and nutrient uptake by maize plant were determined. The experiment was conducted by using complete randomized design (CRD) with three replicates. The result indicated that germination percentage, Shoot length, leaf length, root length, number of leaves, and plant fresh weight were reduced by 37, 39, 39, 32 and 59% respectively at 0.75 mg kg-1 of CdSO4 concentration but when maize seeds inoculated with Bacillus pumilus significantly increased the germination percentage, shoot length, leaf length, number of leaves, plant fresh weight at different concentrations of CdSO4. Moreover, the plant protein were significantly increased by 60% in T6 (0.25 mg kg-1 of CdSO4 + inoculated seed) and Peroxidase dismutase (POD) was also significantly higher by 346% in T6 (0.25 mg kg-1 of CdSO4 + inoculated seed), however, the Superoxide dismutase (SOD) was significantly higher in T5 (0.75 mg kg-1 of CdSO4 + uninoculated seed) and was 769% higher as compared to control. The Cd contents in Bacillus pumilus inoculated maize roots and shoots were decreased. The present investigations indicated that the inoculation of maize plant with Bacillus pumilus can help maize plants to withstand Cd stress but higher concentration of Cd can harm the plant. The Bacillus pumilus has good potential to remediate Cd from soil, and also have potential to reduce the phyto availability and toxicity of Cd.


Assuntos
Bacillus pumilus/metabolismo , Cádmio/toxicidade , Poluentes do Solo/toxicidade , Zea mays/efeitos dos fármacos , Bacillus pumilus/patogenicidade , Biodegradação Ambiental , Cádmio/metabolismo , Sementes/efeitos dos fármacos , Sementes/metabolismo , Sementes/microbiologia , Poluentes do Solo/metabolismo , Estresse Fisiológico , Zea mays/metabolismo , Zea mays/microbiologia
7.
APMIS ; 129(10): 587-597, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34117658

RESUMO

Periodontological grafts are materials used in dentistry to regenerate lost gingival soft tissues or bone parts. In the case of direct contact with blood, the possibility of disease transmission from the source to the patient is high. This source can be an animal or a human. Therefore, the sterilization of grafts before implanting to the patient is of significant importance. The purpose of this study was to evaluate gamma radiation and microwave sterilization processes from microbiological and sterility perspectives and to compare the effectiveness of these two sterilization methods. Grafts were irradiated with 2, 4, 5, 10, 25 and 50 kGy doses of gamma radiation. Another group of same materials was irradiated by microwave for 1, 2, 3 and 4 min at 24,500 MHz and 900 W. Gamma radiation and microwave sterilization methods were evaluated as successful at minimum doses as 5 kGy and 3 min, respectively. Both gamma and microwave sterilization successfu lly sterilized periodontological grafts coded as PBG1, HBG1, HL1, PDG1, MBG3, MDG2 and PDG3. Moreover, microwave sterilization can be used as an alternative novel method to gamma radiation sterilization.


Assuntos
Bacillus pumilus/efeitos da radiação , Raios gama , Micro-Ondas , Esterilização/métodos , Transplantes/efeitos da radiação , Perda do Osso Alveolar/cirurgia , Animais , Bacillus pumilus/crescimento & desenvolvimento , Transplante Ósseo/métodos , Colágeno/efeitos da radiação , Contagem de Colônia Microbiana , Relação Dose-Resposta à Radiação , Cavalos , Humanos , Suínos , Transplantes/microbiologia
8.
Int J Biol Macromol ; 183: 1119-1135, 2021 Jul 31.
Artigo em Inglês | MEDLINE | ID: mdl-33974923

RESUMO

Lectins are renowned hemagglutinins and multivalent proteins with a well known quality for sugar-binding specificity that participate significantly in invertebrate defense functions. Studies on biological activity of lectin from coleopteran insect are very scarce. In this study, lectin from the hemolymph in the grub of banana pest, Odoiporus longicollis was subjected to purification, biochemical and functional characterizations. The lectin was purified by PEG precipitation and ion-exchange chromatography using Q-Sepharose as a matrix. The purified lectin showed hemagglutination activity against rat erythrocytes, heat-labile, cation independent and insensitive to EDTA. Further, the carbohydrate affinity of this lectin was found with mannitol, adonitol, L-arabinose, L-rhamnose, D-galactose and sorbitol. The native form of purified lectin was calculated as 360 kDa by FPLC system. Denatured gel electrophoresis of the purified lectin consisted of five distinct polypeptides with molecular weights approximately 160, 60, 52, 40 and 38 kDa, respectively. The amino acid sequences obtained through peptide mass fingerprinting analysis exhibited homologies to the known conserved regions of galactose binding lectins. Further, the purified lectin exhibited bacterial inhibition with LPS from Serratia marcescens. In addition, isolated lectin also exerted bacterial agglutination, antibacterial and anti-proliferative activity against Mycobacterium smegmatis, Bacillus pumilus and Neuro 2a cell line, respectively.


Assuntos
Antibacterianos/farmacologia , Antineoplásicos/farmacologia , Besouros/metabolismo , Galectinas/farmacologia , Animais , Antibacterianos/isolamento & purificação , Antineoplásicos/isolamento & purificação , Bacillus pumilus/efeitos dos fármacos , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Cromatografia por Troca Iônica , Relação Dose-Resposta a Droga , Galectinas/isolamento & purificação , Humanos , Proteínas de Insetos/isolamento & purificação , Proteínas de Insetos/farmacologia , Camundongos , Mycobacterium smegmatis/efeitos dos fármacos , Ratos
9.
Toxins (Basel) ; 13(5)2021 04 21.
Artigo em Inglês | MEDLINE | ID: mdl-33919181

RESUMO

Zearalenone (ZEA) is a mycotoxin widely occurring in many agricultural commodities. In this study, a purified bacterial isolate, Bacillus sp. S62-W, obtained from one of 104 corn silage samples from various silos located in the United States, exhibited activity to transform the mycotoxin ZEA. A novel microbial transformation product, ZEA-14-phosphate, was detected, purified, and identified by HPLC, LC-MS, and NMR analyses. The isolate has been identified as belonging to the genus Bacillus according to phylogenetic analysis of the 16S rRNA gene and whole genome alignments. The isolate showed high efficacy in transforming ZEA to ZEA-14-phosphate (100% transformation within 24 h) and possessed advantages of acid tolerance (work at pH = 4.0), working under a broad range of temperatures (22-42 °C), and a capability of transforming ZEA at high concentrations (up to 200 µg/mL). In addition, 23 Bacillus strains of various species were tested for their ZEA phosphorylation activity. Thirteen of the Bacillus strains showed phosphorylation functionality at an efficacy of between 20.3% and 99.4% after 24 h incubation, suggesting the metabolism pathway is widely conserved in Bacillus spp. This study established a new transformation system for potential application of controlling ZEA although the metabolism and toxicity of ZEA-14-phosphate requires further investigation.


Assuntos
Bacillus/metabolismo , Zearalenona/metabolismo , Bacillus/genética , Bacillus pumilus/genética , Bacillus pumilus/metabolismo , Cromatografia Líquida de Alta Pressão , Cromatografia Gasosa-Espectrometria de Massas , Fosforilação , Filogenia , RNA Ribossômico 16S/genética , Alinhamento de Sequência
10.
J Anim Physiol Anim Nutr (Berl) ; 105(4): 797-805, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-33675272

RESUMO

An increasing number of Bacillus strains have been developed for use as animal feed additives. The aim of the current work was to evaluate the impacts of Bacillus pumilus fsznc-09 in growth performance, organs development, blood constituents, genes expression of growth and immune in spleen and microbial communities in jejunum of weanling mice. The results showed that the body weight of mice in BP1 group increased significantly (p < 0.05) after feeding Bacillus pumilus fsznc-09. Compared with control group, the feed conversion ratio of BP1 and BP2 groups showed 13.57% (p < 0.05) and 9.64% improvements, respectively. The lengths of large intestine, small intestine in BP1 group were significantly increased (p < 0.05). While compared with control group, the organ indexes in BP1 and BP2 group did not differ significantly. Compared with control group, the activities of serum total superoxide dismutase (T-SOD), alkaline phosphatase (AKP), lysozyme (LZM) in BP1 group and T-SOD, AKP in BP2 group were significantly increased (p < 0.05). Compared with control group, the expressions of ghrelin-2 (Ghrl-2) and insulin-like growth factor 1 (Igf1) in BP1 group were significantly increased (p < 0.05). Compared with control group, the expressions of interleukin-6 (IL-6), nitric oxide synthase (INOS), tumour necrosis factor-α (TNF-α), interleukin-1ß (IL-1ß) in BP1 group and the expressions of IL-6, INOS, TNF-α, IL-1ß and interferon alpha 11 (Ifna11) in BP2 group were slightly decreased. Moreover, compared with control group, the diversity of intestinal flora and relative abundance of potentially probiotics (e.g., Bifidobacterium, Bacillus) in BP1 and BP2 groups were increased. While compared with control group, the relative abundance of the potentially pathogenic bacterium (e.g., Staphylococcus) was reduced. The relative abundances of dominant species in BP1 (Lactobacillus johnsonii) and BP2 (Lactobacillus reuteri) groups were also higher than control group (Lactobacillus intestinalis). In conclusion, Bacillus pumilus fsznc-09 might improve the growth performance and immunity of mice.


Assuntos
Bacillus pumilus , Microbioma Gastrointestinal , Probióticos , Ração Animal/análise , Animais , Lactobacillus , Camundongos
11.
Microb Cell Fact ; 20(1): 40, 2021 Feb 08.
Artigo em Inglês | MEDLINE | ID: mdl-33557838

RESUMO

BACKGROUND: Microbial surfactants called biosurfactants, thanks to their high biodegradability, low toxicity and stability can be used not only in bioremediation and oil processing, but also in the food and cosmetic industries, and even in medicine. However, the high production costs of microbial surfactants and low efficiency limit their large-scale production. This requires optimization of management conditions, including the possibility of using waste as a carbon source, such as food processing by-products. This papers describes the production and characterization of the biosurfactant obtained from the endophytic bacterial strain Bacillus pumilus 2A grown on various by-products of food processing and its potential applications in supporting plant growth. Four different carbon and nitrogen sources, pH, inoculum concentration and temperature were optimized within Taguchi method. RESULTS: Optimization of bioprocess within Taguchi method and experimental analysis revealed that the optimal conditions for biosurfactant production were brewer's spent grain (5% w/v), ammonium nitrate (1% w/v), pH of 6, 5% of inoculum, and temperature at 30 °C, leading to 6.8 g/L of biosurfactant. Based on gas chromatography-mass spectrometry and Fourier transform infrared spectroscopy analysis produced biosurfactant was determined as glycolipid. Obtained biosurfactant has shown high and long term thermostability, surface tension of 47.7 mN/m, oil displacement of 8 cm and the emulsion index of 69.11%. The examined glycolipid, used in a concentration of 0.2% significantly enhanced growth of Phaseolus vulgaris L. (bean), Raphanus L. (radish), Beta vulgaris L. (beetroot). CONCLUSIONS: The endophytic Bacillus pumilus 2A produce glycolipid biosurfactant with high and long tem thermostability, what makes it useful for many purposes including food processing. The use of brewer's spent grain as the sole carbon source makes the production of biosurfactants profitable, and from an environmental point of view, it is an environmentally friendly way to remove food processing by products. Glycolipid produced by endophytic Bacillus pumilus 2A significantly improve growth of Phaseolus vulgaris L. (bean), Raphanus L. (radish), Beta vulgaris L. (beetroot). Obtained results provide new insight to the possible use of glycolipids as plant growth promoting agents.


Assuntos
Bacillus pumilus , Beta vulgaris/crescimento & desenvolvimento , Endófitos , Phaseolus/crescimento & desenvolvimento , Raphanus/crescimento & desenvolvimento , Tensoativos , Bacillus pumilus/química , Bacillus pumilus/metabolismo , Endófitos/química , Endófitos/metabolismo , Tensoativos/isolamento & purificação , Tensoativos/metabolismo , Tensoativos/farmacologia
12.
Curr Microbiol ; 78(4): 1142-1149, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33608775

RESUMO

One marine bacteria Bacillus pumilus was isolated using allura red as ε-poly-L-lysine (ε-PL) secretion indicator. But actually the product was identified as poly-γ-L-diaminobutanoic acid (γ-PAB) by ionization-time-of-flight mass spectrometry, not coproduced with ε-PL. The polymerization degree of γ-PAB was 4-22, namely short-chain γ-PAB, compared with that in S. celluloflavus, and it exhibited stronger inhibitory activities against yeasts than long-chain γ-PAB but weaker activities against bacteria. The fermentative behavior of B. pumilus was investigated, and the γ-PAB production was 38.6 mg/L in shake flask and was enhanced to 284.2 mg/L in 5-L bioreactor by a pH control strategy. Interestingly, the suitable pH for B. pumilus to produce γ-PAB was 4.8, different from 4.0 for current Streptomyces strains, which suggests a potential new metabolic mechanism in B. pumilus as a novel γ-PAB producer. No studies on short-chain γ-PAB production in bacteria have been reported previously and we considered that this is a new discovery in the field of homopolymer research.


Assuntos
Bacillus pumilus , Streptomyces , Aminoácidos , Fermentação , Polilisina
13.
Biotechnol Lett ; 43(3): 691-700, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33386499

RESUMO

OBJECTIVES: To search for new alkaliphilic cellulases and to improve their efficiency on crystalline cellulose through molecular engineering RESULTS: Two novel cellulases, BpGH9 and BpGH48, from a Bacillus pumilus strain were identified, cloned and biochemically characterized. BpGH9 is a modular endocellulase belonging to the glycoside hydrolase 9 family (GH9), which contains a catalytic module (GH) and a carbohydrate-binding module belonging to class 3 and subclass c (CBM3c). This enzyme is extremely tolerant to high alkali pH and remains significantly active at pH 10. BpGH48 is an exocellulase, belonging to the glycoside hydrolase 48 family (GH48) and acts on the reducing end of oligo-ß1,4 glucanes. A truncated form of BpGH9 and a chimeric fusion with an additional CBM3a module was constructed. The deletion of the CBM3c module results in a significant decline in the catalytic activity. However, fusion of CBM3a, although in a non native position, enhanced the activity of BpGH9 on crystalline cellulose. CONCLUSIONS: A new alkaliphilic endocellulase BpGH9, was cloned and engineered as a fusion protein (CBM3a-BpGH9), which led to an improved activity on crystalline cellulose.


Assuntos
Bacillus pumilus/enzimologia , Proteínas de Bactérias , Celulases , Proteínas Recombinantes de Fusão , Bacillus pumilus/genética , Proteínas de Bactérias/química , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Celulases/química , Celulases/genética , Celulases/metabolismo , Celulose/metabolismo , Estabilidade Enzimática , Escherichia coli , Quênia , Lagos/microbiologia , Engenharia de Proteínas , Proteínas Recombinantes de Fusão/química , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/metabolismo
14.
Poult Sci ; 100(3): 100871, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33516480

RESUMO

Probiotics are being developed as alternatives to antibiotic growth promoters. The aim of the study was to investigate the effects of 2 novel strains of Bacillus pumilus and Bacillus subtilis on production, intestinal microbiota, gut health, and immunity of broilers raised under suboptimal conditions. Day-old chicks (Cobb 500, n = 2,073) were randomly assigned into 6 groups: Con group (group fed with basal diet), Ab group (group treated with virginiamycin), groups treated with 2 levels of B. pumilus (low dose: 3 × 108 cfu/kg of feed [BPL] and high dose: 1 × 109 cfu/kg [BPH]), and groups treated with 2 levels of B. subtilis (low dose: 3 × 108 cfu/kg [BSL] and high dose: 1 × 109 cfu/kg [BSH]). Production parameters were recorded weekly. Cecal tonsils and content as well as ileum samples were collected on day 14 and day 42. Cecal tonsils were used to sort T-regulatory cells (CD4+CD8-CD25+ and CD4+CD8+CD25+) to study expression of IL-10 and interferon gamma, whereas cecal content was used for bacterial culture. Ileum samples were used to measure gene expression of tight junction proteins, mucin, and cytokines. BW and feed intake increased in the Ab, BPL, BSL, and BSH groups compared with the Con group between day 35 and day 42. The CD4+CD8-CD25+ cells expressed high levels of IL-10 in the BSH group on day 14 and in the BPL, BSL, and BSH groups on day 42 and high levels of interferon gamma in the BPL, BSL, and BSH groups on day 14 and in the BSL and BSH groups on day 42. The expression of IL-10 and interferon gamma in CD4+CD8+CD25+ cells was higher only in the BSH group on day 14 and day 42. Cecal bacterial populations of genera, Lactobacillus (day 14 and day 42) and Clostridium (day 14), were higher in the BSH group. Expression of tight junction protein increased significantly in the ileum on day 14 in the BPL (occludin, zona occludens 1 [ZO-1]), BSL (occludin, ZO-1), and BSH (occludin, ZO-1, junctional adhesion molecule 2 [JAM-2]) groups compared with that in the Con group and declined in all groups except in the BSH group (occludin, ZO-1, JAM-2) on day 42. Expression of MUC2 and IL-17F increased in all groups on day 14 and remained high on day 42 in the BSL and BSH groups. Taken together, both Bacillus probiotics altered the intestinal and immune activities, particularly on day 14, suggesting beneficial influence of probiotics.


Assuntos
Bacillus pumilus , Bacillus subtilis , Galinhas , Microbioma Gastrointestinal , Probióticos , Animais , Bacillus pumilus/fisiologia , Bacillus subtilis/fisiologia , Galinhas/crescimento & desenvolvimento , Galinhas/imunologia , Galinhas/microbiologia , Dieta/veterinária , Trato Gastrointestinal/microbiologia , Trato Gastrointestinal/fisiologia , Distribuição Aleatória
15.
Physiol Plant ; 171(3): 388-399, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33034388

RESUMO

It has been reported that drought stress adversely affects the growth and yield of Glycyrrhiza uralensis, Chinese liquorice, in agricultural production. Bacillus pumilus, an important plant growth-promoting bacterium, play a significant role in improving plant tolerance to abiotic stress. However, the role of Bacillus pumilus G5 in resisting drought stress is largely unknown. In the present study, we found that drought stress significantly inhibited the growth and reduced the biomass of G. uralensis seedlings by restraining C- and N-metabolism, while this could be effectively reversed by B. pumilus G5 inoculation. Specifically, B. pumilus G5 significantly increased the content of primary metabolites such as soluble sugar, soluble protein, and free amino acids by regulating the C and N metabolic processes in G. uralensis seedlings. Moreover, B. pumilus G5 increased the content of glycyrrhizic acid, one of the important secondary metabolites, likely mediated through the increased content of primary metabolites and by recovering the expression of three key enzymes, HMGR, SQS, and ß-AS, in the biosynthesis of glycyrrhizic acid. Interestingly, the regulating effect of B. pumilus G5 inoculation on promoting the accumulation of glycyrrhizic acid and increasing the expression of synthesis-related genes is spatially selective. In summary, our findings suggest that B. pumilus G5 could alleviate adverse effects induced by drought stress on the growth of G. uralensis seedlings by regulating C- and N-metabolisms that further triggered the accumulation of secondary metabolites, and this finally improved the drought tolerance of cultivated G. uralensis seedlings.


Assuntos
Bacillus pumilus , Glycyrrhiza uralensis , Secas , Metabolismo Secundário , Plântula
16.
Anal Biochem ; 612: 113975, 2021 01 01.
Artigo em Inglês | MEDLINE | ID: mdl-32966803

RESUMO

The interaction of three proteins, viz. Bovine Serum Albumin (BSA), Human Serum Albumin (HSA) and Hen Egg White Lysozyme (HEWL) with gold nanoparticles (GNPs) is investigated using surface plasmon resonance (SPR) spectroscopy, fluorescence spectroscopy and circular dichroism (CD). Size and morphology of the samples was established using Transmission Electron Microscopy (TEM) and stability studies was established using zeta potential analysis. The stability of protein-GNP complex was found to be greater than that of individual protein as well as individual GNPs. Also HEWL-GNP complex was more stable compared to the other protein complexes. Absorbance of proteins increases with increase in gold nanoparticle concentration due to the extension of peptide strands of protein and decrease in hydrophobicity of gold nanoparticles. A ground state complex is also formed which is evident from the moderate shift observed in the absorbance peaks. Apparent association constant was also determined from the absorption spectra and was found to be maximum for HEWL and minimum for HSA. Gold nanoparticles were found to act as quenchers and reduced the protein fluorescence intensity. Binding constant and number of binding sites were found to be maximum for HEWL and minimum for HSA. The temperature dependent fluorescence studies were also performed to calculate the thermodynamic parameters and to determine the nature of interaction between the proteins and gold nanoparticles. The circular dichroism studies elucidate the reason behind the maximum binding for HEWL and minimum binding for HSA. TGA analysis determined the thermal stability of the samples. Fluorescence lifetime studies indicate static quenching of proteins. Antibacterial activity of protein-gold nanoparticles was studied against four pathogens, viz. Bacillus pumilus, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. HEWL exhibits a tunable antimicrobial activity against Pseudomonas aeruginosa due to the maximum binding of HEWL with gold nanoparticles. The study proposes a novel method for adjusting the antibacterial activity of HEWL against Pseudomonas aeruginosa when the resistance of this pathogen is a major issue in the chemotherapy of many infectious diseases. Thus the combination therapy of protein-gold nanoparticles could prove to be a new approach in medical field in the near future.


Assuntos
Antibacterianos/química , Antibacterianos/farmacologia , Ouro/química , Nanopartículas Metálicas/química , Muramidase/química , Soroalbumina Bovina/química , Albumina Sérica Humana/química , Animais , Bacillus pumilus/efeitos dos fármacos , Sítios de Ligação , Bovinos , Galinhas , Dicroísmo Circular , Escherichia coli/efeitos dos fármacos , Humanos , Muramidase/metabolismo , Ligação Proteica , Pseudomonas aeruginosa/efeitos dos fármacos , Soroalbumina Bovina/metabolismo , Albumina Sérica Humana/metabolismo , Espectrometria de Fluorescência , Espectrofotometria Ultravioleta , Staphylococcus aureus/efeitos dos fármacos , Ressonância de Plasmônio de Superfície , Termodinâmica , Termogravimetria
17.
Microbiol Res ; 242: 126616, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33115624

RESUMO

Rice (Oryza sativa L.) growth and productivity has been negatively affected due to high soil salinity. However, some salt-tolerant plant growth-promoting bacteria (ST-PGPB) enhance crop growth and reduce the negative impacts of salt stress through regulation of some biochemical, physiological, and molecular features. Total thirty six ST-PGPB were isolated from sodic soil of eastern Uttar Pradesh, India, and screened for salt tolerance at different salt (NaCl) concentrations up to 2000 millimolar (mM). Out of thirty-six, thirteen strains indicated better growth and plant growth properties (PGPs) in NaCl amended medium. Among thirteen, one most effective Bacillus pumilus strain JPVS11 was molecularly characterized, which showed potential PGPs, such as indole-3-acetic acid (IAA),1-aminocyclo propane-1-carboxylicacid (ACC) deaminase activity, P-solubilization, proline accumulation and exopolysaccharides (EPS) production at different concentrations of NaCl (0 -1200 mM). Pot experiment was conducted on rice (Variety CSR46) at different NaCl concentrations (0, 50, 100, 200, and 300 mM) with and without inoculation of Bacillus pumilus strain JPVS11. At elevated concentrations of NaCl, the adverse effects on chlorophyll content, carotenoids, antioxidant activity was recorded in non-inoculated (only NaCl) plants. However, inoculation of Bacillus pumilus strain JPVS11 showed positive adaption and improve growth performance of rice as compared to non-inoculated in similar conditions. A significant (P < 0.05) enhancement plant height (12.90-26.48%), root length (9.55-23.09%), chlorophyll content (10.13-27.24%), carotenoids (8.38-25.44%), plant fresh weight (12.33-25.59%), and dry weight (8.66-30.89%) were recorded from 50 to 300 mM NaCl concentration in inoculated plants as compared to non-inoculated. Moreover, the plants inoculated with Bacillus pumilus strain JPVS11showed improvement in antioxidant enzyme activities of catalase (15.14-32.91%) and superoxide dismutase (8.68-26.61%). Besides, the significant improvement in soil enzyme activities, such as alkaline phosphatase (18.37-53.51%), acid phosphatase (28.42-45.99%), urease (14.77-47.84%), and ß-glucosidase (25.21-56.12%) were recorded in inoculated pots as compared to non-inoculated. These results suggest that Bacillus pumilus strain JPVS11 is a potential ST-PGPB for promoting plant growth attributes, soil enzyme activities, microbial counts, and mitigating the deleterious effects of salinity in rice.


Assuntos
Bacillus pumilus/fisiologia , Oryza/crescimento & desenvolvimento , Oryza/microbiologia , Desenvolvimento Vegetal , Estresse Salino/fisiologia , Plantas Tolerantes a Sal/crescimento & desenvolvimento , Plantas Tolerantes a Sal/microbiologia , Solo/química , Antioxidantes , Bacillus pumilus/classificação , Bacillus pumilus/genética , Bacillus pumilus/isolamento & purificação , Bactérias/classificação , Bactérias/genética , Bactérias/isolamento & purificação , Clorofila , Cianeto de Hidrogênio/metabolismo , Ácidos Indolacéticos , Fixação de Nitrogênio , Fosfatos/metabolismo , Prolina/metabolismo , Salinidade , Tolerância ao Sal/fisiologia , Sementes/microbiologia , Sideróforos/metabolismo , Microbiologia do Solo , Estresse Fisiológico
18.
Ci. Rural ; 51(6)2021. graf, ilus, tab
Artigo em Inglês | VETINDEX | ID: vti-31169

RESUMO

The excessive use of agrochemicals negatively impacts the environment, making the development of sustainable technologies for the reduction of contaminants in soil necessary. Hexazinone is the herbicide most used for sugarcane crops and persists in the environment. Moreover, its main route of degradation in the soil is through microorganisms. Therefore, six microorganisms were selected that presented growth in the presence of the herbicide; SCR1 - Microbacterium arborescens; SCR2 - Bacillus pumilus; SCM3 - Stenotrophomonas maltophilia; SCM4 - Bacillus cereus; SCM5A - M. arborescens; and SCM5B - B. safensis. A test was performed to evaluate the ability of each lineage in phosphate solubilization. For the Ca3(PO4)2 solubilization test, the strains that showed the best results were B. pumilus and S. maltophilia. Subsequently, the inoculants were prepared and the concentrations after plating were 2.71 × 109 CFU mL-1 for B. pumilus, 1.02 × 109 CFU mL-1 for S. maltophilia, and 1.14 × 1010 CFU mL-1 for a combination of the two strains. These were satisfactory values for use as inoculants.(AU)


O uso de agroquímicos resulta em impactos ambientais e torna-se necessário o emprego de tecnologias sustentáveis para diminuição de contaminantes no solo. O hexazinona é o herbicida mais utilizado para a cultura da cana-de-açúcar, e apresenta persistência no ambiente. A principal via de degradação no solo é por meio de microrganismos. Com isso, selecionou-se seis microrganismos que apresentaram crescimento na presença do herbicida: SCR1 - Microbacterium arborescens; SCR2 - Bacillus pumilus; SCM3 - Stenotrophomonas maltophilia; SCM4 - Bacillus cereus; SCM5A - M. arborescens; SCM5B - Bacillus safensis. Foi realizado um teste para avaliar a habilidade de cada linhagem na solubilização de fosfatos, e no caso da solubilização de Ca3(PO4)2, as linhagens que apresentaram melhores resultados foram B. pumilus e S. maltophilia. Posteriormente, os inoculantes foram preparados e a concentração após plaqueamento de 2,71x109 UFC mL-1 para B. pumilus, 1,02x109 UFC mL-1 para S. maltophilia e consórcio com as duas linhagens 1,14x1010 UFC mL-1 apresentaram valores satisfatórios para utilização como inoculantes.(AU)


Assuntos
Tratamento do Solo/métodos , Microbiologia do Solo , Poluentes do Solo/antagonistas & inibidores , Bacillus pumilus/crescimento & desenvolvimento , Stenotrophomonas maltophilia/crescimento & desenvolvimento
19.
Biomolecules ; 10(11)2020 11 02.
Artigo em Inglês | MEDLINE | ID: mdl-33147876

RESUMO

The important role of miRNA in cell proliferation and differentiation has raised interest in exogenous ribonucleases (RNases) as tools to control tumour-associated intracellular and extracellular miRNAs. In this work, we evaluated the effects of the RNase binase from Bacillus pumilus on small non-coding regulatory RNAs in the context of mouse RLS40 lymphosarcoma inhibition. In vitro binase exhibited cytotoxicity towards RLS40 cells via apoptosis induction through caspase-3/caspase-7 activation and decreased the levels of miR-21a, let-7g, miR-31 and miR-155. Intraperitoneal injections of binase in RLS40-bearing mice resulted in the retardation of primary tumour growth by up to 60% and inhibition of metastasis in the liver by up to 86%, with a decrease in reactive inflammatory infiltration and mitosis in tumour tissue. In the blood serum of binase-treated mice, decreases in the levels of most studied miRNAs were observed, excluding let-7g, while in tumour tissue, the levels of oncomirs miR-21, miR-10b, miR-31 and miR-155, and the oncosuppressor let-7g, were upregulated. Analysis of binase-susceptible miRNAs and their regulatory networks showed that the main modulated events were transcription and translation control, the cell cycle, cell proliferation, adhesion and invasion, apoptosis and autophagy, as well as some other tumour-related cascades, with an impact on the observed antitumour effects.


Assuntos
Endorribonucleases/farmacologia , Neoplasias Hepáticas/tratamento farmacológico , Linfoma não Hodgkin/tratamento farmacológico , Ribonucleases/farmacologia , Animais , Apoptose/efeitos dos fármacos , Bacillus pumilus/enzimologia , Diferenciação Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Endorribonucleases/química , Endorribonucleases/genética , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Humanos , Neoplasias Hepáticas/terapia , Linfoma não Hodgkin/genética , Linfoma não Hodgkin/patologia , Camundongos , MicroRNAs/antagonistas & inibidores , MicroRNAs/genética , Ribonucleases/química , Ribonucleases/genética
20.
World J Microbiol Biotechnol ; 36(12): 181, 2020 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-33164140

RESUMO

Although the use of degrading-bacteria is one of the most efficient methods for the bioremediation of polluted sites, detection, selection and proliferation of the most efficient and competing bacteria is still a challenge. The objective of this multi-stage research was to investigate the effects of the selected bacterial strains on the degradation of anthracene, florentine, naphthalene, and oil, determined by biochemical tests. In the first stage, using the following tests: (a) biosurfactant production (emulsification, oil spreading, number of drops, drop collapse, and surface tension), (b) biofilm production, (c) activity of laccase enzyme, and (d) exopolysaccaride production, the three bacterial strains with the highest degrading potential including Bacillus pumilus, B. aerophilus, and Marinobacter hydrocarbonoclasticus were chosen. In the second stage using the following tests: (a) bacterial growth, (b) laccase enzyme activity, and (c) biosurfactant production (emulsification, oil spreading, and collapse of droplet) the degrading ability of the three selected bacterial strains plus Escherichia coli were compared. Different bacterial strains were able to degrade anthracene, florentine, naphthalene, and oil by the highest rate, three days after inoculation (DAI). However, M. hydrocarbonoclasticus showed the highest rate of florentine degradation. Although with increasing pollutant concentration the degrading potential of the bacterial strains significantly decreased, M. hydrocarbonoclasticus was determined as the most efficient bacterial strain.


Assuntos
Bactérias/crescimento & desenvolvimento , Bactérias/metabolismo , Poluentes Ambientais/química , Hidrocarbonetos Policíclicos Aromáticos/química , Antracenos/química , Bacillus/crescimento & desenvolvimento , Bacillus/metabolismo , Bacillus pumilus/crescimento & desenvolvimento , Bacillus pumilus/metabolismo , Bactérias/isolamento & purificação , Biodegradação Ambiental , Biofilmes , Biocombustíveis/análise , Escherichia coli/crescimento & desenvolvimento , Escherichia coli/metabolismo , Lacase/metabolismo , Marinobacter/crescimento & desenvolvimento , Marinobacter/metabolismo , Naftalenos/química , Polissacarídeos Bacterianos/metabolismo , Tensoativos/metabolismo
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