Flower-like 3D MoS2 microsphere/2D C3N4 nanosheet composite for highly sensitive electrochemical sensing of nitrite.

Nitrite pollution poses a serious threat to human health and the environment. In this study, a reliable and selective electrochemical (EC) sensor was developed for the quantitative determination of nitrite by combining flower-like three-dimensional (3D) MoS2 microspheres with two-dimensional (2D) C3N4 nanosheets. Benefiting from the synergistic effects of MoS2 and C3N4, the 3D MoS2/2D C3N4 nanocomposite displayed numerous active sites, a 3D mesoporous structure, high conductivity and excellent catalytic activity. The 3D MoS2/2D C3N4-modified glassy carbon electrode (GCE) exhibited a superior electrocatalytic activity toward nitrite oxidation, with a wider linear detection range (0.1-1100 µM), a lower detection limit (LOD) (0.065 µM, S/N = 3), outstanding stability, remarkable reproducibility and strong selectivity. Furthermore, the nitrite EC sensor was successfully applied to detect actual food and environmental samples involving sausage, pickled vegetables, river water and tap water, thus demonstrating the potential of the prepared 3D MoS2/2D C3N4/GCE for food analysis and environmental monitoring.

Acid-assisted ultrasonic preparation of nitrogen-doped MXene quantum dots for the efficient fluorescence "off-on-off" detection of Zn(II) in water and oxalic acid in vegetables.

A novel fluorescence "off-on-off" probe was presented to detect Zn(II) and oxalic acid (OA) based on nitrogen-doped MXene quantum dots (N-MQDs), which were synthesized by an ultrasound approach at room temperature with nitric acid and ethylenediamine. These N-MQDs displayed small size (<10 nm), water dispersibility, and good photoluminescence. Furthermore, the N-MQDs showed an selective response towards Zn(II) through fluorescence enhancement, with a limit of detection (LOD) calculated as 0.127 µM in the linear range of 0-20 µM. Then, the fluorescence of N-MQDs/Zn(II) system could be selectively quenched after adding OA, with an effective response in the range from 0 to 20 µM (LOD: 0.883 µM). The fluorescence "turn-on" and "turn-off" properties of N-MQDs were resulted from the intramolecular charge transfer (ICT) of Zn(II) and the coordination between OA and Zn(II), respectively. This sensing platform was successfully applied for Zn(II) and OA detection in actual environmental and vegetable samples.

Cobalt molybdate hollow spheres decorated graphitic carbon nitride sheets for electrochemical sensing of dimetridazole.

In the present work, a cobalt molybdate (CoMoO4) hollow spheres-incorporated graphitic carbon nitride (g-CN) composite is prepared for the electrochemical detection of dimetridazole (DZ). The synergistic effect between the hollow-structured CoMoO4 and g-CN nanosheets facilitates the transportation of electrons through kinetic barriers, thereby providing a high electrical conductivity with increased electroactive sites. The proposed CoMoO4@g-CN-modified electrode displayed a wide linear range (0.001-492.77 µM) and a lower detection limit (LOD: 0.4 nM) for the determination of DZ through the amperometry (i-t) method. In addition, the CoMoO4@g-CN-modified electrode achieved good operational stability, anti-interfering ability (five-fold excess amount of co-interfering compounds) and reproducibility. These results demonstrate the increased electrocatalytic activity of CoMoO4@g-CN modified glassy carbon electrode (GCE) towards the detection of DZ in food samples with satisfactory recovery ranges.

Electrochemical sensor for sensitive nitrite and sulfite detection in milk based on acid-treated Fe3O4@SiO2 nanoparticles.

In this work, a simple electrochemical sensing platform based on acid-treated Fe3O4@SiO2 nanoparticles was successfully prepared for nitrite and sulfite detection. Fe3O4@SiO2 nanoparticles were synthesized through the sol-gel and hydrothermal methods. Fe3O4@SiO2 presented positive charges after acid treatment, which could enhance the electrostatic attraction between Fe3O4@SiO2 and nitrite and sulfite. The Fe3O4@SiO2(acid-treated) modified magnetic glassy carbon electrode (MGCE) was applied to detect nitrite and sulfite using differential pulse voltammetry and cyclic voltammetry. Under optimized conditions, the developed electrochemical sensor presented good analytical properties for nitrite and sulfite detection with detection limits of 3.33 µmol/L and 31.57 µmol/L, respectively. The good recoveries varied from 85.18% to 111.02%, with a relative standard deviation of 0.23-4.80%. Furthermore, the Fe3O4@SiO2(acid-treated) modified MGCE showed better selectivity, reproducibility, and repeatability in nitrite and sulfite detection. Therefore, this proposed electrochemical sensor provides a new method for developing a nitrite and sulfite detection sensor.

"Fluorescence-wavelength" label-free POCT tandem with "fluorescence-photothermal" nanobody-immunosensor for detecting BSA and ß-lactoglobulin.

Two carbon dots (CDs) (λEm = 525 nm, G-CDs and λEm = 640 nm, R-CDs) were synthesized from citric acid and urea. The bovine serum albumin (BSA) responsiveness of the R-CDs was used to develop a "fluorescence-wavelength" label-free point of care testing (POCT) for the detection of the milk quality marker BSA with the detection limit (LOD) of 4.89 µg/mL for fluorescence mode and 3.38 µg/mL for wavelength mode. In addition, R-CDs were found to have hydroxyl radical (·OH)-dependent fluorescence quenching properties, and a "fluorescence-photothermal" immunosensor based on nanobodies was constructed by introducing the fluorescence signal of R-CDs@BSA and the photothermal signal of oxTMB for the detection of ß-lactoglobulin (ß-LG) with the LOD of 0.034 ng/mL for fluorescence mode and 0.075 ng/mL for photothermal mode. The tandem detection of POCT and immunosensor enables the simultaneous and highly sensitive detection of BSA and ß-LG after only simple dilution of less than 5 µL of sample.

Preparation of size-tunable Fe3O4 magnetic nanoporous carbon composites by MOF pyrolysis regulation for magnetic resonance sensing of aflatoxin B1 with excellent anti-matrix effect.

Magnetic nanoporous materials represent a new emerging category of magnetic materials for construction of magnetic resonance sensors. In this study, we adopted the metal-organic framework materials, MIL-101(Fe), as the precursor to prepare series nanoporous-carbon-Fe3O4 (NPC-Fe3O4) composites. Results showed that Fe3O4 were uniformly distributed in MIL-101(Fe) and the size of MNP was precisely tuned at different pyrolysis temperatures, conferring the optimal NPC-Fe3O4-450 °C composite with dramatically improved T2 relaxivity. The NPC-Fe3O4-450 °C composite was modified with antibodies and antigens, respectively, for detection of aflatoxin B1 in various food samples with complicated matrix. Range from 0.010 ng mL-1 to 2.0 ng mL-1, extreme low detection limit of 5.0 pg mL-1, and satisfied recoveries were successfully achieved, indicating excellent anti-matrix effect. These findings offer a new dimension to engineer novel magnetic materials with improved relaxivity for simple and easy sensing of food hazards in complicated food matrix without any purification or separation procedures.

Monitoring levamisole in food and the environment with high selectivity using an electrochemical chiral sensor comprising an MOF and molecularly imprinted polymer.

We used an enhanced recognition strategy to fabricate a novel levamisole-detecting chiral electrochemical sensor featuring a metal-organic framework (MOF) combined with a molecularly imprinted polymer (MIP). We first synthesised a Cu/Zn-[benzene-1,3,5-tricarboxylic acid] (Cu/Zn-BTC) MOF as the molecular immobilisation and signal-amplifying unit, and then prepared the MIP (molecular recognition unit) using levamisole as the template on a glassy carbon electrode modified with Cu/Zn-BTC. We obtained a composite chiral sensor with enhanced recognition capability for levamisole after template removal. Using the templated sites as the switch and K3[Fe(CN)6]/K4[Fe(CN)6] as a probe, we established a new method for detecting levamisole in meat products and water bodies. The linear detection range and detection limit of our chiral sensor are 5 to 6000 × 10-11 mol/L and 1.65 × 10-12 mol/L, respectively. Moreover, the sensor exhibited 93.8-109.0% recovery in the detection of levamisole in chicken and other real samples.

Zinc-doped carbon quantum dots-based ratiometric fluorescence probe for rapid, specific, and visual determination of tetracycline hydrochloride.

In this work, a rapid, specific, and visual ratiometric fluorescence probe was constructed for tetracycline hydrochloride (TCH) determination based on zinc-doped carbon quantum dots (Zn-CDs). In the presence of TCH, the blue fluorescence at 440 nm originating from Zn-CDs was quenched, and the green fluorescence at 515 nm stemming from TCH was enhanced. The inner filter effect (IFE) and the chelation between Zn and tetracycline are the main mechanisms for the conversion of spectra. The spectrum and color change completed and stabilized within 1 min, indicating the possibility of real-time detection of TCH. The detection range for TCH is 0.1-50 µM, and the low detection limit is 61.1 nM. In addition, Zn-CDs-based test strips were successfully applied to direct visual identification of TCH in actual samples of river water and milk, indicating the possibility of their practical application.

Hinge-like paper-based dual-channel enzyme-free ratiometric fluorescent microfluidic platform for simultaneous visual detection of carbaryl and glyphosate.

On-site multi-pesticide residues detection is particularly urgent and challenging. Here, we fabricated an enzyme-free ratiometric fluorescent detection system in combination with a hinge-like dual-channel 3D microfluidic paper analytical device (3D µPAD) for simultaneous visual detection of carbaryl and glyphosate. Blue-emission 1-naphthol (Em. 470 nm) was hydrolyzed from carbaryl, while yellow-emission 2,3-diaminophenazine (Em. 570 nm) was produced with the aid of Cu2+ for glyphosate sensing. Inner-filter effect between 1-naphthol or 2,3-diaminophenazine and green-emission carbon dots (Em. 510 nm) realized two ratiometric fluorescent detection systems. Remarkable color variation of green-blue for carbaryl (50.00-1100 µΜ) and yellow-green for glyphosate (5.00-600 µΜ) were observed on a dual-channel 3D µPAD without crosstalk. Their detection limits were 1.11 and 0.63 µΜ, respectively. The strategy realized simultaneous visual detection of carbaryl and glyphosate in food/herbal with excellent accuracy (spiked recoveries, 91.00-107.2%), high precision (RSD ≤ 8.43%), and superior selectivity.

Smartphone-based electrochemical sensor for cost-effective, rapid and on site detection of chlorogenic acid in herbs using biomass-derived hierarchically porous carbon synthesized by a soft-hard dual template method.

To achieve excellent germplasm resource screening and ensure the quality control of herbal tea raw material, it is important to establish a cost-effective, rapid, and on site quantitative detection method for their bioactive constituents. We developed a smartphone-operated sensor for electrochemical detection of chlorogenic acid (CGA) using hierarchically porous carbon (DSiFPC), synthesized through a soft-hard dual template strategy with tannin acid as a carbon source, silica colloid as a hard template, and Pluronic F127 as a soft template. The DSiFPC modified glassy carbon electrode sensor showed excellent electrocatalytic ability towards CGA, with a wide linear range of 0.03-1 µM and a low limit of detection of 6.2 nM. It was successfully applied for detecting CGA in dried flowers of Lonicera japonica. Furthermore, a portable sensor utilizing a DSiFPC modified screen-printed electrode was employed for on site detection of CGA in fresh Eucommia ulmoides leaves, yielding satisfactory recoveries.

Dietary sources of branched-chain fatty acids and their biosynthesis, distribution, and nutritional properties.

Branched-chain fatty acids (BCFAs) consist of a wide variety of fatty acids with alkyl branching of methyl group. The most common BCFAs are the types with one methyl group (mmBCFA) on the penultimate carbon (iBCFA) or the antepenultimate carbon (aiBCFA). Long-chain mmBCFAs are widely existing in animal fats, milks and are mostly derived from bacteria in the diet or animal digestive system. Recent studies show that BCFAs benefit human intestinal health and immune homeostasis, but the connection between their content, distribution in the human and their nutritional functions are not well established. In this paper, we reviewed BCFAs from various dietary sources focused on their molecular species. The BCFAs biosynthesis in bacteria, Caenorhabditis elegans, mammals and their distribution in human tissues are summarized. This paper also discusses the nutritional properties of BCFAs including influences on intestinal health, immunoregulatory effects, anti-carcinoma, and anti-obesity activities, by highlighting the most recent research progress.

A biosensor encompassing fusarinine C-magnetic nanoparticles and aptamer-red/green carbon dots for dual-channel fluorescent and RGB discrimination of Campylobacter and Aliarcobacter.

The diarrhea pathogens Campylobacter and Aliarcobacter are similar in morphology and their leading symptoms, making them difficult to be differentially diagnosed. Herein, we report a biosensor with two modules to differentiate the genera-representative species of C. jejuni and A. butzleri. Module 1 was fusarinine C-decorated magnetic nanoparticles; module 2 consisted of C. jejuni-specific aptamer modified with red-emitting carbon dots (CDs) and A. butzleri-specific aptamer-modified green-emitting CDs, consisting non-interfering dual-fluorescence detection channels. Module 1 was used to selectively capture C. jejuni and A. butzleri from an un-cultured sample, and the specific CDs in module 2 would then recognize and bind to their counterpart bacteria when subjected to the collected module 1-bacteria complex. By measuring the fluorescence intensities from the CDs-bound bacteria, the abundance of each bacterium could be differentially indicated. This biosensor exhibited a wide detection range of up to 1 × 107 CFU/mL and the lowest limit of detection (LOD) of 1 CFU/mL, for each bacterium. Thus, the biosensor with dual-fluorescent channels facilitated a culture-independent, ultrasensitive and discriminative detection of C. jejuni and A. butzleri. Remarkably, this fluorescent detection could be transformed into RGB color indication to render the visual discrimination. After the biosensor was coupled with microfluidics, a biosensing platform was developed, which could render fluorescent and RGB differentiation of the two bacteria in human stool or chicken broilers, achieving a LOD of 5 CFU/mL and turnaround time of 65 min. This work established the first biosensor-based methodology for the discriminative detection of Campylobacter and Aliarcobacter in real samples.

Using stable carbon isotope ratio analysis to detect adulteration in red yeast rice dietary supplements.

Red yeast rice (RYR) is marketed as a dietary supplement because it contains natural 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitors (statins), including monacolin K. However, there is concern that some RYR supplements may be adulterated with the pharmaceutical drug lovastatin to enhance health claims. We have developed an optimized method to isolate monacolin K/lovastatin from complex RYR dietary supplement matrices to then test for adulteration in RYR supplements using stable carbon isotope (δ13C) analysis. Samples were initially screened for monacolin K/lovastatin using liquid chromatography with mass spectrometric detection (LC-MS). To ensure the extraction process did not affect the measured isotopic values (i.e., isotopic fractionation effects), neat lovastatin standards were spiked into two types of blank RYR matrices (powder and gel). The monacolin K/lovastatin peaks were detected using high performance liquid chromatography with ultraviolet detection (HPLC-UV) and isolated using fraction collection. Residual matrix components were removed from targeted fractions by solid phase extraction (SPE) using graphitized carbon black cartridges. The resulting isolates were then analyzed using elemental analyzer-isotope ratio mass spectrometry (EA-IRMS) to measure δ13C values. The δ13C values of the extracted lovastatin standards were compared to their respective neat lovastatin δ13C values and demonstrated negligible isotopic fractionation effects. Using this optimized clean up method and carbon isotope analysis, thirty-one samples were screened. Eight RYR dietary supplement samples had >0.8 mg/g of monacolin K/lovastatin, our minimum threshold for analyzing samples using this method. Four of these eight samples had δ13C values greater than -28.3‰, a previously proposed cutoff value for natural monacolin K, indicating likely adulteration. Additionally, five RYR powder samples were analyzed as part of a collaborative study using in-house methods from two laboratories and the data shows acceptable agreement in the δ13C values of monacolin K/lovastatin (differences ranging from ±0.02‰ to ±0.76‰). This optimized method represents a robust, reproducible procedure for detecting lovastatin adulteration in dietary supplements with minimal isotopic fractionation.

MOFs-derived Co3O4@MnO2@Carbon dots with enhanced nanozymes activity for photoelectrochemical detection of cancer cells in whole blood.

Nanozymes have attracted widespread attention, and rationally designing high-activity nanozymes to improve their application performance are a long-term objective. Herein, taking metal-organic frameworks-derived Co3O4 polyhedron with large surface area and high porosity as nanoconfinement carriers, Co3O4@MnO2@CDs polyhedron was successfully synthesized by the room-temperature reduction of MnO4- ions and physical load of carbon dots (CDs). Through cancer cells-triggered double antibody sandwich strategy, the Co3O4@MnO2@CDs polyhedron were introduced to the TiO2 nanoparticle (NPs) modified electrode, leading to the decreased photocurrent. The Co3O4@MnO2@CDs polyhedron can not only quench the photocurrent of TiO2 NPs, also act as nanozymes to catalyze precipitates. Moreover, the precipitates can not only reduce the photoelectrochemical (PEC) response, also increase the quenching capacity of the Co3O4@MnO2@CDs polyhedron. Additionally, the steric hindrance effect of the Co3O4@MnO2@CDs-Ab conjugates further weaken the photocurrent. Based on the multifunctional Co3O4@MnO2@CDs polyhedron, the proposed PEC biosensor for the detection of A549 cancer cells exhibits a wide linear range from 102 to 106 cells/mL and a low detection limit of 11 cells/mL. Furthermore, this strategy can differentiate between lung cancer patients and healthy individuals. The designed multifunctional Co3O4@MnO2@CDs nanozymes provide a new horizon for PEC detection of cancer cells, and may have great potential in early clinical diagnosis and biomedical research.

MOF-derived porous carbon nanozyme-based flexible electrochemical sensing system for in situ and real-time monitoring of H2O2 released from cells.

A novel flexible electrochemical sensor based on porous carbon nanosheets (PCNSs) nanozyme has been constructed for in situ and real-time monitoring of H2O2 released by cells. The PCNSs are prepared with the integration of thermal transformation, thermal activation and sonochemical exfoliation by using zeolitic imidazolate frameworks as template. The PCNSs exhibit high electrical conductivity, electrochemical activity and peroxidase-like catalytic properties, which is beneficial to H2O2 assay. With the transfer printing method, the flexible electrochemical sensor is obtained, which has excellent performances for H2O2 electrochemical detecting with wide linear range from 1 µM to 20 mM and a low detection limit of 0.76 µM. Owing to the great biocompatibility, the flexible sensor guarantees the growth of living cells for 72 h and realizes in situ and real-time monitoring the release of H2O2 from HeLa cells. The strategy of porous nanozyme preparation and flexible sensor construction provided a promising way for in situ and real-time assay of small molecules in the cellular microenvironment.

Dual strategy for 13C-Metabolic flux analysis of central carbon and energy metabolism in Mammalian cells based on LC-isoMRM-MS.

Central carbon and energy metabolism are the most concerned metabolic pathways in 13C-Metabolic flux analysis (13C-MFA). However, some α-keto acids, ribonucleoside triphosphate (NTPs) and deoxyribonucleoside triphosphate (dNTPs) involved in central carbon and energy metabolism pathways were unstable or reactive, leading to inaccurate metabolic flux analysis. To achieve accurate 13C-MFA of central carbon and energy metabolism, we proposed a dual strategy for the detection of 101 metabolites in glucose metabolism pathways. N-Methylphenylethylamine (MPEA) was utilized for derivatization of 4 carboxyl (α-keto acids) and 8 phosphate metabolites (NTPs and dNTPs). After derivatization, the MPEA derivatives were investigated to be stable for 4 weeks under 4 °C and detected with high intensity in ∼104 cells. On the other hand, we analyzed an additional 89 metabolites in central carbon and energy metabolic pathways were directly analyzed by liquid chromatography tandem mass spectrometry (LC-MRM-MS). The limit of detection (LODs) of our method were as low as 0.05 ng/mL and the linear range was at least two orders of magnitude with determination coefficient (R2) > 0.9701. The relative standard divisions (RSDs) of intra- and inter-day of 95% metabolites were below 20%. In addition, the isotope list of 82 detected metabolites in central carbon and energy metabolism were generated according to isotopologues and isotopomers for each metabolite resulting from 13C incorporation. Accurate assessment of mass isotopomer distributions (MIDs) of intracellular 13C-labeled metabolites was achieved in [U-13C]-glucose cultured HepG2 cells by our dual strategy. Finally, we performed MID analysis of 101 metabolites in central carbon and energy metabolism. Overall, this dual method is reproducible and robust for application on 13C-MFA and has a great potential for studying clinical isotope labeled samples.

A novel carbon-based material with titanium and zirconium ions etched on hollow mesoporous carbon tubes for specific capture of phosphopeptides and exosomes.

The analysis of low abundance phosphopeptides in organisms and specific capture exosomes are crucial for unraveling the pathogenesis of diseases. For this reason, titanium-zirconium ions and highly biocompatible dopamine and polyimide tubes (PITs) were introduced, and a novel carbon-based material with titanium and zirconium ions etched on hollow mesoporous carbon tubes (HMCT), denoted as G@C@Ti-Zr-HMCT, comes into being after high-temperature calcination. Attributing to the tightly bound titanium and zirconium ions to HMCT and the high carbon content of the polydopamine carbonaceous layer, G@C@Ti-Zr-HMCT displays satisfactory capability of enriching phosphopeptides with satisfactory detection limit (0.2 fmol), extraordinary selectivity (1:2000), and good loading capacity (100 µg/mg). In addition, 25 phosphopeptides related to 25 phosphoproteins from the serum of Parkinson's disease (PD) patients and 30 phosphopeptides attributed to 26 phosphoproteins from the serum of healthy individuals were enriched by G@C@Ti-Zr-HMCT, respectively. In addition, bioinformatics analysis of the above results revealed that PD were associated with serine, threonine, and leucine of high frequency, blood coagulation in BP, Golgi apparatus and mitochondrial outer membrane in CC, and heparin binding in MF. Moreover, the phospholipid bilayer of exosomes and metallic titanium and zirconium ions interact to produce the following results: this highly biocompatible carbon-based material was successfully applied to capture exosomes, which offers a promising platform for isolating exosomes. To sum up, these delightful results confirmed without doubt that G@C@Ti-Zr-HMCT has enjoyed a splendiferous future in the specific capture of phosphopeptides and exosomes isolation.

Carrageenan-based multifunctional packaging films containing Zn-carbon dots/anthocyanin derived from Kohlrabi peel for monitoring quality and extending the shelf life of shrimps.

Carrageenan-based active/intelligent packaging films containing anthocyanin and ZnO-doped CD (Zn-CD) from purple Kohlrabi peels were prepared for freshness monitoring and shelf-life extension of shrimp, and the influence of additives on the films' physical, functional, and structural properties was investigated. The films showed excellent UV blocking ability (85.2% of UV-A and 99.4% of UV-B) and high antioxidant effect (∼99% for ABTS and âˆ¼ 58.6% for DPPH radical scavenging activity) and showed strong antibacterial activity to stop the growth (100%) of L. monocytogenes and to reduce the growth of E. coli by 8.1 log CFU/mL after 12 h of incubation. In shrimp packaging experiments, the films were evident in the freshness monitoring, reduced spoilage, and increased shelf life. This study suggests that next-generation biopolymer films impregnated with biomass-derived CDs and natural colorants will provide broad directions for ensuring safety and extending shelf life to meet the accelerating demand for packaging products.

Bell pepper derived nitrogen-doped carbon dots as a pH-modulated fluorescence switching sensor with high sensitivity for visual sensing of 4-nitrophenol.

Recently, converting bio-waste into bio-asset and implementing a portable sensing instrument for pollutant monitoring has been highly desirable and challenging. Herein, biomass-derived nitrogen-doped carbon dots (CDs) are prepared hydrothermally and emit blue fluorescence (470 nm) with a high quantum yield of 23.2%. Significantly, CDs can serve as a pH-modulated fluorescence switching nano-sensor to detect 4-NP from 0.054 to 68 µM with low detection limit (LOD, 54 nM) and limit of quantification (LOQ, 181 nM) based on inner filter effect. Moreover, the satisfactory recovery of 101.8-107.5% is gained in practical sample monitoring. Furthermore, a smartphone-integrated optosensing device with CDs-based film is developed for detecting 4-NP with LOD and LOQ of 0.110 µM and 0.350 µM. Concomitantly, the practicability of this device is further validated in several crop samples with satisfactory recovery rates of 101.6-108.6%. Therefore, this work provides a reliable way and a prospective application for on-site 4-NP monitoring in food.

An in-situ blocking strategy for improved anti-interference inspection of AFB1 based on hollow covalent organic framework capsules with commodious and undisturbed microenvironment.

This work proposed an in-situ blocking strategy for improved anti-interference and signal-amplified inspection of hazards via constructing hollow covalent organic framework (HCOF) capsules. An aptamer-FRET nanoprobe integrated with carbon dots and CuS was introduced into the micro-capsule as signal indicator to demonstrate the proof-of-concept. The HCOF was successfully prepared by removing the metal-organic frameworks (MOF) core from the MOF@COF that had been preloaded with the nanoprobes under mild conditions. Meanwhile, the hydrophobic surface of HCOF enhanced the adsorption and penetration of aflatoxin B1 (AFB1) into the capsule to interact with the nanoprobes. This strategy was applied to detect AFB1 in food samples, achieving a linear response of 1-300 nM along with a detection limit of 0.3 nM. Selectivity test verified that the prepared sensing platform could specifically recognize AFB1 without complex sample pretreatment. This study provides new ideas for improved anti-interference inspection of hazards against complex sample matrix.