Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 80.869
Filtrar
1.
Anal Chim Acta ; 1208: 339824, 2022 May 22.
Artigo em Inglês | MEDLINE | ID: mdl-35525586

RESUMO

Luminescent organic molecules are of important realistic significance to the human health and ecological environment due to their fascinating applications. Here we report the design and synthesis of luminescent organic-molecules by introducing two or four NH-pyrazolate groups as mercury-binding moieties to aromatic cores. Interestingly, the new aromatic tetraphenylene-bridged multi-NH-pyrazoles exhibit strong fluorescence in both aggregate and solid state and constitutes highly selective proof-of-concept luminescent sensor for Hg(II) ion among various competitive transition-metal ions in both organic and mixed solutions via metal-nitrogen binding. Especially, the present sensor including two NH-pyrazolyl groups showed an extremely high sensitivity with low limit of detection of 7.26 and 3.67 nM. The proposed design strategy provides a wide scope for the construction of unique turn-on sensors with substantial potential in the sense of heavy metal pollution in enviromental water samples.


Assuntos
Mercúrio , Metais Pesados , Corantes Fluorescentes/química , Humanos , Íons , Espectrometria de Fluorescência
2.
Anal Chim Acta ; 1208: 339825, 2022 May 22.
Artigo em Inglês | MEDLINE | ID: mdl-35525587

RESUMO

Atherosclerosis (AS) is the main cause of coronary heart disease, cerebral infarction and peripheral vascular disease, which is an important disease threatening human health. Abnormal levels of protein phosphorylation are closely related to the occurrence and development of diseases. Herein, the ratiometric fluorescence nanosensor (PCN/W- B@BSA) was prepared by using metal-organic frameworks (PCN-224) and fluorescent nanocluster wool-balls, which was applied for ratiometric fluorescence imaging of protein phosphorylation level in the AS mice model. Specific recognition of phosphorylation sites was achieved via specific interaction between active center Zr(IV) and phosphate. Using the two-photon property of porphyrin, the background is significantly reduced, and the sensitivity of imaging analysis is improved by combining with ratio imaging. Bovine serum albumin (BSA) was used to modify the surface of the nanosensor to reduce the non-specific adsorption and improve the biocompatibility of the nanosensor. Finally, the fluorescence nanosensor was successfully apply to fluorescence imaging of protein phosphorylation level in AS mice model, and the results showed that the protein phosphorylation level in the AS mice model was lower than that of the normal mice. The present study provides suitable fluorescence tool for further revealing phosphorylation related signaling pathways and disease mechanisms.


Assuntos
Aterosclerose , Técnicas Biossensoriais , Animais , Aterosclerose/diagnóstico por imagem , Fluorescência , Corantes Fluorescentes , Camundongos , Fosforilação , Carbonitrila de Pregnenolona , Soroalbumina Bovina , Espectrometria de Fluorescência/métodos
3.
Anal Chim Acta ; 1208: 339831, 2022 May 22.
Artigo em Inglês | MEDLINE | ID: mdl-35525589

RESUMO

Food additives are essential to guarantee processed foods' safety throughout its journey from workshops or factories to shops or catering establishment and eventually to consumers. As one of the commonly-used food additives, nitrites upon reaction with amines would generate highly toxic nitrosamines (e.g., N,N-diethylnitrosamine, DEN) as inadvertent byproducts resulted from food processing or preparation which are known to cause hepatotoxicity and even cancer. Hence detecting nitrosamine-induced acute liver injury accurately would be conducive to planning optimal treatment and avoid any further deterioration. Herein we design an activatable probe (BHC-Lut) that can release the drug luteolin for therapy and the chromophore (BHC-OH) for NIR-II fluorescence/optoacoustic imaging upon being triggered by hepatic biomarker hydrogen peroxide. In the probe BHC-Lut, benzoindolium heptamethine cyanine with NIR-II fluorescent emission is adopted as the chromophore scaffold, the incorporation of triethylene glycol into benzoindolium ensures sufficient water solubility and enhances biocompatibility of the probe, and luteolin is coupled onto the chromophore via boronate linkage that acts as both H2O2-responsive unit and the fluorescence quencher. The probe itself is weakly emissive. In the presence of H2O2, the boronate bond is cleaved, and the chromophore BHC-OH and the drug luteolin are released, which produces evident NIR-II fluorescent/optoacoustic signals for imaging and wields therapeutic effect respectively. The probe BHC-Lut has been used in DEN-induced hepatic injury model in mice, and the results evince BHC-Lut's capability for in-situ biomarker-activatable detection and imaging of the acute liver injury site as well as in-situ biomarker-triggered drug release for therapy.


Assuntos
Técnicas Fotoacústicas , Animais , Biomarcadores , Liberação Controlada de Fármacos , Corantes Fluorescentes/química , Aditivos Alimentares , Peróxido de Hidrogênio , Fígado/diagnóstico por imagem , Luteolina , Camundongos , Imagem Óptica , Técnicas Fotoacústicas/métodos
4.
J Chem Phys ; 156(17): 174901, 2022 May 07.
Artigo em Inglês | MEDLINE | ID: mdl-35525636

RESUMO

We investigate the local viscosity of a polymer glass around its glass transition temperature by using environment-sensitive fluorescent molecular rotors embedded in the polymer matrix. The fluorescence of the rotors depends on the local viscosity, and measuring the fluorescence intensity and lifetime of the probe therefore allows us to measure the local free volume in the polymer glass when going through the glass transition. This also allows us to study the local viscosity and free volume when the polymer film is put under an external stress. We find that the film does not flow homogeneously but undergoes shear banding that is visible as a spatially varying free volume and viscosity.


Assuntos
Corantes Fluorescentes , Polímeros , Vidro , Espectrometria de Fluorescência , Viscosidade
5.
J Chem Phys ; 156(17): 174202, 2022 May 07.
Artigo em Inglês | MEDLINE | ID: mdl-35525668

RESUMO

Fluorescence-encoded infrared (FEIR) spectroscopy is a vibrational spectroscopy technique that has recently demonstrated the capability of single-molecule sensitivity in solution without near-field enhancement. This work explores the practical experimental factors that are required for successful FEIR measurements in both the single-molecule and bulk regimes. We investigate the role of resonance conditions by performing measurements on a series of coumarin fluorophores of varying electronic transition frequencies. To analyze variations in signal strength and signal to background between molecules, we introduce an FEIR brightness metric that normalizes out measurement-specific parameters. We find that the effect of the resonance condition on FEIR brightness can be reasonably well described by the electronic absorption spectrum. We discuss strategies for optimizing detection quality and sensitivity in bulk and single-molecule experiments.


Assuntos
Nanotecnologia , Vibração , Corantes Fluorescentes/química , Espectrometria de Fluorescência/métodos , Espectrofotometria Infravermelho/métodos
6.
Bioorg Med Chem Lett ; 68: 128762, 2022 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-35490954

RESUMO

γ-glutamyl transpeptidase (GGT) is a kind of cell-surface enzyme that is overexpressed in many cancer cells. It is of great significance to develop an ideal tool for the diagnosis of GGT-rich cancer cells. Here, we reported a simple-structured but effective imaging probe for the detection of GGT activity. In the presence of GGT, the γ-glutamyl linkage could be cleaved specifically to produce amino-substituted product, resulting in significant fluorescence enhancement at 578 nm. Moreover, we successfully employed the probe to monitor GGT activity in HepG2 cells. We envisaged that such a simple but effective imaging tool could improve the practical applications for bioimaging.


Assuntos
Corantes Fluorescentes , gama-Glutamiltransferase , Fluorescência , Células Hep G2 , Humanos , Água
7.
J Phys Chem A ; 126(18): 2788-2793, 2022 May 12.
Artigo em Inglês | MEDLINE | ID: mdl-35502941

RESUMO

H2S is an important signal molecule in living systems and related with many physiological processes and diseases. Rapid detection of H2S, hence, is important for studying physiological processes and early diagnosis of diseases. Deep insight into the sensing mechanism is significant and inspiring for the design and modification of high-efficiency H2S probes. The current study has theoretically investigated the recognition and fluorescence mechanism of a newly reported high-efficiency H2S probe. The recognition mechanism is determined to be the reaction between the probe and HS- anion, the rationality of which is further confirmed from the fluorescence property of the recognition product. The non-fluorescence property of the probe attributes to a photoinduced electron transfer process, and the turn-on fluorescence upon exposure to H2S exhibits an intramolecular charge transfer property according to frontier molecular orbital analysis.


Assuntos
Corantes Fluorescentes , Sulfeto de Hidrogênio , Transporte de Elétrons , Fluorescência , Sulfeto de Hidrogênio/análise
8.
Anal Chim Acta ; 1210: 339879, 2022 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-35595366

RESUMO

The fluctuation of the halide ion concentration in human serum is of a high clinical implication. The measurement of Cl- in human serum samples is important because Cl- is a major constituent of human serum and certain Cl- levels are indicators of many acute diseases. Real-time monitoring of Br- levels in human serum samples from patients ingesting Br- salt-based antiepileptic drugs is important to regulate potential adverse drug effects. In this study, we developed a fluorescent probe for the discriminative sensing of Cl- and Br- using a naphthalimide-benzimidazole conjugate-based Ag+ complex (L1-Ag+ complex) by exploiting the strong interaction between Ag+ and halide ions with selectivity control by pH variation. The probe exhibited a dual function of detecting Cl- and Br- at pH levels of 4.5 and 8.0, respectively. Under this two pH conditions, the probe showed a high sensitivity (limit of detection (LOD) = 43 and 5 µM for Cl- and Br-, respectively) and selectivity toward Cl- and Br-. Importantly, the probe could be applied in the quantification of Cl- and Br- levels in human serum samples under two conditions: Cl- in normal human serum and Br- in the serum-mimicking that of epileptic patients during medication.


Assuntos
Corantes Fluorescentes , Humanos , Concentração de Íons de Hidrogênio , Íons , Limite de Detecção
9.
Sci Rep ; 12(1): 8520, 2022 May 20.
Artigo em Inglês | MEDLINE | ID: mdl-35595810

RESUMO

Breast-conserving surgery has become the preferred treatment method for breast cancer. Surgical margin assessment is performed during surgery, as it can reduce local recurrence in the preserved breast. Development of reliable and lower-cost ex vivo cancer detection methods would offer several benefits for patient care. Here, a practical and quantitative evaluation method for the ex vivo fluorescent diagnosis of breast lesions was developed and confirmed through a three-step clinical study. Gamma-glutamyl-hydroxymethyl rhodamine green (gGlu-HMRG) has been reported to generate fluorescence in breast lesions. Using this probe, we constructed a reliable and reproducible procedure for the quantitative evaluation of fluorescence levels. We evaluated the reliability of the method by considering reproducibility, temperature sensitivity, and the effects of other clinicopathological factors. The results suggest that the fluorescence increase of gGlu-HMRG is a good indicator of the malignancy of breast lesions. However, the distributions overlapped. A 5 min reaction with this probe could be used to distinguish at least part of the normal breast tissue. This method did not affect the final pathological examination. In summary, our results indicate that the methods developed in this study may serve as a feasible intraoperative negative-margin assessment tool during breast-conserving surgery.


Assuntos
Neoplasias da Mama , Margens de Excisão , Neoplasias da Mama/diagnóstico por imagem , Neoplasias da Mama/cirurgia , Feminino , Corantes Fluorescentes , Humanos , Mastectomia Segmentar , Reprodutibilidade dos Testes , gama-Glutamiltransferase
10.
Nat Commun ; 13(1): 2475, 2022 May 05.
Artigo em Inglês | MEDLINE | ID: mdl-35513404

RESUMO

Ultra-multiplexed fluorescence imaging requires the use of spectrally overlapping fluorophores to label proteins and then to unmix the images of the fluorophores. However, doing this remains a challenge, especially in highly heterogeneous specimens, such as the brain, owing to the high degree of variation in the emission spectra of fluorophores in such specimens. Here, we propose PICASSO, which enables more than 15-color imaging of spatially overlapping proteins in a single imaging round without using any reference emission spectra. PICASSO requires an equal number of images and fluorophores, which enables such advanced multiplexed imaging, even with bandpass filter-based microscopy. We show that PICASSO can be used to achieve strong multiplexing capability in diverse applications. By combining PICASSO with cyclic immunofluorescence staining, we achieve 45-color imaging of the mouse brain in three cycles. PICASSO provides a tool for multiplexed imaging with high accessibility and accuracy for a broad range of researchers.


Assuntos
Corantes Fluorescentes , Imagem Óptica , Animais , Camundongos , Microscopia de Fluorescência/métodos , Proteínas , Coloração e Rotulagem
11.
J Phys Chem A ; 126(19): 2899-2908, 2022 May 19.
Artigo em Inglês | MEDLINE | ID: mdl-35502789

RESUMO

The ability to tune the optoelectronic properties of quantum dots (QDs) makes them ideally suited for the use as fluorescence sensing probes. The vast structural diversity in terms of the composition and size of QDs can make designing a QD for a specific sensing application a challenging process. Quantum chemical calculations have the potential to aid this process through the characterization of the properties of QDs, leading to their in silico design. This is explored in the context of QDs for the fluorescence sensing of dopamine based upon density functional theory and time-dependent density functional theory (TDDFT) calculations. The excited states of hydrogenated carbon, silicon, and germanium QDs are characterized through TDDFT calculations. Analysis of the molecular orbital diagrams for the isolated molecules and calculations of the excited states of the dopamine-functionalized quantum dots establish the possibility of a photoinduced electron-transfer process by determining the relative energies of the electronic states formed from a local excitation on the QD and the lowest QD → dopamine electron-transfer state. The results suggest that the Si165H100 and Ge84H64 QDs have the potential to act as fluorescent markers that could distinguish between the oxidized and reduced forms of dopamine, where the fluorescence would be quenched for the oxidized form. The work contributes to a better understanding of the optical and electronic behavior of QD-based sensors and illustrates how quantum chemical calculations can be used to inform the design of QDs for specific fluorescent sensing applications.


Assuntos
Pontos Quânticos , Dopamina , Transporte de Elétrons , Fluorescência , Corantes Fluorescentes , Pontos Quânticos/química
12.
Nat Commun ; 13(1): 2533, 2022 May 09.
Artigo em Inglês | MEDLINE | ID: mdl-35534485

RESUMO

Metabolic distribution of fatty acid to organelles is an essential biological process for energy homeostasis as well as for the maintenance of membrane integrity, and the metabolic pathways are strictly regulated in response to environmental stimuli. Herein, we report a fluorescent fatty acid probe, which bears an azapyrene dye that changes its absorption and emission features depending on the microenvironment polarity of the organelle into which it is transported. Owing to the environmental sensitivity of this dye, the distribution of the metabolically incorporated probe in non-polar lipid droplets, medium-polarity membranes, and the polar aqueous regions, can be visualized in different colors. Based on density scatter plots of the fluorophore, we demonstrate that the degradation of triacylglycerols in lipid droplets occurs predominantly via lipolysis rather than lipophagy in nutrition-starved hepatocytes. This tool can thus be expected to significantly advance our understanding of the lipid metabolism in living organisms.


Assuntos
Ácidos Graxos , Corantes Fluorescentes , Ácidos Graxos/metabolismo , Corantes Fluorescentes/metabolismo , Gotículas Lipídicas/metabolismo , Metabolismo dos Lipídeos/fisiologia , Lipólise/fisiologia
13.
Biosens Bioelectron ; 210: 114330, 2022 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-35567882

RESUMO

Numerous polymeric agents have been widely applied in biology and medicine by virtue of the facile chemical modification, feasible nano-engineering approaches and fine-tuned pharmacokinetics. To endow polymeric imaging agents with ability to monitor and measure subtle molecular or cellular alterations at diseased sites, activatable polymeric probes that can elicit signal changes in response to biomolecular interactions or the analytes of interest have to be developed. Herein, this review aims to provide a systemic interpretation and summarization of the design methodology and imaging utility of recently emerged activatable polymeric probes. An introduction of activatable probes allowing for precise imaging and classification of polymeric imaging agents is reported first. Then, we give a detailed discussion of the contemporary design approaches toward activatable polymeric probes in diverse imaging modes for the detection of various stimuli and their imaging applications. Finally, current challenges and future advances are discussed and highlighted.


Assuntos
Técnicas Biossensoriais , Técnicas Fotoacústicas , Corantes Fluorescentes/química , Luminescência , Imagem Molecular/métodos , Imagem Óptica , Técnicas Fotoacústicas/métodos , Polímeros
14.
ChemistryOpen ; 11(5): e202200055, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35543213

RESUMO

Accurate monitoring of methylglyoxal (MGO) at cell and living level was crucial to reveal its role in the pathogenesis of diabetes since MGO was closely related to diabetes. Herein, a ratiometric fluorescence strategy was constructed based on the capture probe 2,3-diaminonaphthalene (DAN) for the specific detection of MGO. Compared to the fluorescent probes with a single emission wavelength, the ratiometric mode by monitoring two emissions can effectively avoid the interference from the biological background, and provided additional self-calibration ability, which can realize accurate detection of MGO. The proposed method showed a good linear relationship in the range of 0-75 µm for MGO detection, and the limit of detection was 0.33 µm. DAN responded to MGO with good specificity and was successfully applied for detecting the ex vivo MGO level in plasma of KK-Ay mice as a type II diabetes model. Besides, the prepared DAN test strip can be visualized for rapid semi-quantitative analysis of MGO using the naked eye. Furthermore, human skin fibroblasts and HeLa cells were utilized for exogenous MGO imaging, and ex vivo MGO imaging was performed on tissues of KK-Ay mice. All results indicated that the DAN-based ratiometric fluorescence probe can be used as a potential method to detect the level of MGO, thus enabling indications for the occurrence of diabetes and its complications.


Assuntos
Diabetes Mellitus Experimental , Diabetes Mellitus Tipo 2 , Animais , Corantes Fluorescentes , Células HeLa , Humanos , Óxido de Magnésio , Camundongos , Aldeído Pirúvico
15.
Methods Mol Biol ; 2451: 495-503, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35505027

RESUMO

Fluorophores have been conjugated to nanobodies for approximately a decade, for several applications in molecular biology. More recently, photosensitizers have been conjugated to nanobodies for targeted photodynamic therapy (PDT). The most common chemistry is the random conjugation in which commercial fluorophores or photosensitizers contain a N-hydroxysuccinimide ester (NHS ester) group that reacts specifically and efficiently with lysines in the amino acid sequence of the nanobody and with the N-terminal amino groups to form a stable amide bond. Alternatively, maleimide-containing fluorophores or photosensitizers can be used for conjugation to thiols, in a site-directed manner through a cysteine incorporated at the C-terminal of the nanobody. This chapter addresses both conjugation strategies, providing details on the reaction conditions, purification, and characterization of the conjugates obtained.


Assuntos
Fármacos Fotossensibilizantes , Anticorpos de Domínio Único , Ésteres , Corantes Fluorescentes , Ionóforos , Fármacos Fotossensibilizantes/química , Anticorpos de Domínio Único/química
16.
J Am Chem Soc ; 144(18): 8194-8203, 2022 May 11.
Artigo em Inglês | MEDLINE | ID: mdl-35482960

RESUMO

3,6-Carbazole precursors were used to prepare an octaphyrin. The conformation and electronic structure of the system could be modulated through trifluoroacetate (TFA) protonation and BF2 complexation. The resulting nonaromatic macrocyclic complexes, 2-2TFA and 2-2BF2, displayed noteworthy photophysical properties. For instance, the diprotonated species 2-2TFA showed a strong panchromic absorption up to 800 nm, while the bis-BF2-chelated dipyrromethene (BODIPY)-like complex 2-2BF2 exhibited an intense visible absorption feature (ε535nm = 2.1 × 105 M-1 cm-1), as well as a relatively red-shifted emission at 640 nm characterized by a large Stokes shift. It was found that 2-2BF2 could be used to construct a high-quality organic microlaser that functions under optical pumping. The present study highlights the potential utility of expanded porphyrins as possible laser dyes.


Assuntos
Corantes Fluorescentes , Porfirinas , Corantes Fluorescentes/química , Lasers , Conformação Molecular , Porfirinas/química
17.
J Mater Chem B ; 10(18): 3512-3523, 2022 May 11.
Artigo em Inglês | MEDLINE | ID: mdl-35416232

RESUMO

Carbon dot (CD)-based tumor imaging has been proven to be a reliable nanodiagnostic technique. Although abundant types of CDs have been developed, it is still a major challenge to synthesize long-wavelength CDs with high quality and superior repetition due to the complicated synthetic process. Here, stable long-wavelength red-light emission carbon dots (R-CDs) have been synthesized using appropriate carbon sources via a solvothermal method, which enables effective visualization of deep brain glioblastoma (GBM) by a liposome-formulated delivery system. The luminescence phenomenon and structural growth characteristics of R-CDs have been fully investigated and it has been found that R-CDs exhibit different optical behaviors in different pH and solvent environments. In vitro and in vivo models have proved their excellent cell targeting capacity, bioluminescence imaging potential, and biosafety for GBM visualization. Considering their stability and biocompatibility, the in-depth tissue imagining and other extensive applications of R-CDs are strongly recommended.


Assuntos
Neoplasias Encefálicas , Pontos Quânticos , Neoplasias Encefálicas/diagnóstico por imagem , Carbono/química , Corantes Fluorescentes/química , Humanos , Luminescência , Pontos Quânticos/química
18.
Chem Commun (Camb) ; 58(38): 5769-5772, 2022 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-35452065

RESUMO

Acyl chloride is an important functional group in acylation reactions, though its compounds are difficult to store and transport due to their high reactivity. In this research, phenanthro[9,10-d]imidazole is introduced into an acyl chloride agent of TPEPCl to enhance the stability via the delocalization effect. Meanwhile, rarely reported acyl chloride crystals have been achieved which could further prove its stability. The tetraphenylethylene group is also employed to visually monitor the changes of TPEPCl via fluorescent variation with the help of aggregation-induced emission (AIE) characteristics. The reactivity of TPEPCl could be stimulated by a strong alkaline solution or high temperature. Crystallography analyses of acyl TPE-PI derivatives are also discussed and the relationship between the delocalization structure and stability is determined. These findings help to stabilize, control and monitor the reactivities of active functional materials for applications.


Assuntos
Cloretos , Corantes Fluorescentes , Corantes Fluorescentes/química
19.
BMC Neurosci ; 23(1): 21, 2022 03 31.
Artigo em Inglês | MEDLINE | ID: mdl-35361108

RESUMO

BACKGROUND: Microglia, the resident immune cells in the central nervous system, accrue autofluorescent granules inside their cytoplasm throughout their lifespan. In this report, we studied the impacts of autofluorescence on widely used fluorescence-based techniques to study microglia, including flow cytometry, immunofluorescence staining, and live imaging. RESULTS: The failed attempt of using fluorescein isothiocyanate (FITC) conjugated antibody to detect lymphocyte-activation gene 3 protein in microglia prompted us to compare the sensitivity of FITC, phycoerythrin (PE) and allophycocyanin (APC) conjugated antibodies to detect surface protein expression in microglia. We found that PE outperformed FITC and APC as the fluorophore conjugated to antibody for flow cytometry by overcoming the interference from microglia autofluorescence. To identify the location and source of microglia autofluorescence, we did confocal imaging and spectral analysis of microglia autofluorescence on fixed brain tissues, revealing that microglia autofluorescence emitted from cytoplasmic granules and displayed a multi-peak emission spectrum. We recommended removing autofluorescence by lipofuscin removing agents when staining intracellular proteins in microglia with the immunofluorescence techniques. On live brain slices, autofluorescent granules reduced the amplitudes of calcium signals in microglial somata derived from GCaMP6s fluorescence and thus needed to be excluded when selecting regions of interest (ROI). CONCLUSIONS: In conclusion, autofluorescence is a critical factor to consider when designing experiments and interpreting results based on fluorescence-based techniques to study microglia.


Assuntos
Microglia , Ficoeritrina , Citometria de Fluxo/métodos , Imunofluorescência , Corantes Fluorescentes
20.
Anal Chem ; 94(14): 5514-5520, 2022 04 12.
Artigo em Inglês | MEDLINE | ID: mdl-35360906

RESUMO

Hydrogen sulfide (H2S) is an important endogenous gas signal molecule in living system, which participates in a variety of physiological processes. Very recent evidence has accumulated to show that endogenous H2S is closely associated with various cancers and can be regarded as a biomarker of cancer. Herein, we have constructed a new near-infrared fluorescent probe (DCP-H2S) based on isophorone-xanthene dye for sensing hydrogen sulfide (H2S). The probe shows remarkable NIR turn-on signal at 770 nm with a large Stokes shift of 200 nm, together with high sensitivity (15-fold) and rapid detection ability for H2S (4 min). The probe also possesses excellent selectivity for H2S over various other analytes including biothiols containing sulfhydryl (-SH). Moreover, DCP-H2S has been successfully applied to visualize endogenous and exogenous H2S in living cells (293T, Caco-2 and CT-26 cells). In particular, the excellent ability of DCP-H2S to distinguish normal mice and tumor mice is shown, and it is expected to be a powerful tool for detection of H2S in cancer diagnosis.


Assuntos
Corantes Fluorescentes , Sulfeto de Hidrogênio , Animais , Células CACO-2 , Células HeLa , Humanos , Camundongos , Imagem Óptica
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA