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1.
Acta Trop ; 225: 106216, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34717889

RESUMO

The prevalence of Opisthorchis viverrini, a fish-borne zoonotic trematode that can provoke cholangiocarcinoma, is high in the Northeast Thailand. The aim of this study was to determine the prevalence of O. viverrini metacercariae in the cyprinid fish and determine its association of O. viverrini infection among the consumers who regularly buy fish in the markets. A cross-sectional study was conducted in nine provinces covering 20 districts of Thailand, and we examined 778 cyprinoid fish specimens belonging to five species purchased from local markets. Pepsin-HCl digestion method was used to recover O. viverrini metacercariae from fish. In all districts surveyed, O. viverrini metacercariae-positive fish were found with the infection rates ranging from 3.9 to 21.1%. All five fish species studied were positive for O. viverrini metacercariae: Henicorhynchus siamensis (13.7%), Cyclocheilichtys spp. (12.7%), Hampala spp. (8.1%), Systomus spp. (6.9%) and Barbonymus goniatus (5.0%). An average prevalence of O. viverrini infection was 7.1% in the fish consumers surveyed in the markets. The source of fish was determined and our results showed that parasitized fish are sold in markets up to 100 km away from the point of capture, which contributes to the dispersion and maintenance of this helminthiasis. Our results point to the transmission of liver flukes via markets, in spite of many active programs of health education, elimination, prevention and control aimed to reduce O. viverrini infection and subsequent cholangiocarcinoma in the endemic areas of Thailand.


Assuntos
Cyprinidae , Doenças dos Peixes , Opistorquíase , Opisthorchis , Animais , Estudos Transversais , Doenças dos Peixes/epidemiologia , Humanos , Metacercárias , Opistorquíase/epidemiologia , Opistorquíase/veterinária , Prevalência , Tailândia/epidemiologia
2.
Biol Trace Elem Res ; 200(1): 354-363, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33576943

RESUMO

Elements are the shared result of the erosion of rocks in the catchment area and human activities. Nutritional habits, ecological needs, heavy metal concentrations in water and sediment, duration of fishing in the aquatic environment, fishing season, and physicochemical properties of water (salinity, pH, hardness, and temperature) are among the effective factors in the accumulation of heavy metals in various fish organs. In this study, 150 specimens of Rutilus kutum were collected from the southern shores of the Caspian Sea including Astara, Anzali, and Kiashahr in Guilan Province, Farahabad in Mazandaran Province, and Bandar Torkaman in Golestan Province from December 2018 through October 2019. It is possible to predict the metal concentrations using the variables such as fish tissue, sampling region, and season. Akaike information criterion (AIC) was used to select the best regression model. We used fish muscle tissue and Anzali sampling site which were considered reference variables in the regression model. For some elements, a better model is obtained by considering all variables (AIC criterion is its lowest value). The best model obtained for Cu, Mn, and Si was only with region (as a variable). The best model obtained for Sn and Sr only concerns the region and tissue variables. The best model obtained for Sb only related to tissue variable. Using these models, environmental monitoring becomes easier and cheaper. We suggest further studies to be carried out in the shortest possible time along with the least laboratory cost.


Assuntos
Cyprinidae , Metais Pesados , Oligoelementos , Poluentes Químicos da Água , Animais , Mar Cáspio , Monitoramento Ambiental , Humanos , Metais Pesados/análise , Estações do Ano , Oligoelementos/análise , Poluentes Químicos da Água/análise
3.
Sci Total Environ ; 806(Pt 2): 150600, 2022 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-34592296

RESUMO

Fish production from aquaculture and wild captures suffers from the rising risk of climate change. This impacts the livelihoods of fishers and fish farmers by shrinking wild fishery stocks, inland water scarcity, and consequent declines in economic and protein productions. China, feeding the most fish of the world with water-intensive crops, faces challenges of water scarcity but still be premature in developing strategies to adapt to climate change. Here, focusing on methodology development, we quantified the water footprint of fish-farming and economic and protein productions in the baseline year 2014. Then, 29 scenarios of farmed-fish-species composition (FFSC, i.e., tons of each farmed fish species) were developed for the target year 2020. The baseline 2014 shows that fish farming generates an average of 150 billion m3 of water footprint, 4.70 million tons of protein, and 263 billion RMB of economic output (~39 billion USD). Uncertainty optimizations were conducted to generate the optimal FFSC solutions that show a potential to increase fish production by 22%, economic and protein output by 18% and 29%, respectively and simultaneously lower water footprint by 22% to the maximum extent. Nine scenarios that lower wild fishery captures were further examined, with optimal FFSC solution that encourages aquaculture of Grass carp, Bighead Carp, and Silver Carp, and discourages Black carp, Tilapia, Crucian carp, Sea bass, and Wuchang bream. From a methodology aspect, this study pulls back policymakers from only focusing on the short-term economic interest of fish-farming and persuades them to rethink long-term adaptive strategies to climate change from multiple sustainable dimensions.


Assuntos
Conservação dos Recursos Hídricos , Cyprinidae , Animais , Aquicultura , Mudança Climática , Pesqueiros
4.
Sci Total Environ ; 806(Pt 2): 150427, 2022 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-34600207

RESUMO

The emergence of non-native fungal pathogens is a growing threat to global health, biodiversity, conservation biology, food security and the global economy. Moreover, a thorough understanding of the spread and emergence of pathogens among invasive and native host populations, as well as genetic analysis of the structure of co-invasive host populations, is crucial in terms of conservation biology and management strategies. Here we combined extensive catchment sampling, molecular detection tools and genomic signatures to i) assess the prevalence of the rosette agent Sphaerothecum destruens in invasive and native fish populations in contrasting french regions, and ii) characterize the genetic diversity and population structure of its co-invasive and asymptomatic carrier Pseudorasbora parva. Although S. destruens was not detected in all the fish collected its presence in contrasting freshwater ecosystems suggests that the disease may already be widespread in France. Furthermore, our results show that the detection of S. destruens DNA in its asymptomatic carrier P. parva is still limited. Finally, we found that P. parva populations show a homogeneous genetic and geographical structuring, which raises the possibility of the occurrence of successive introduction events in France from their native and invasive range.


Assuntos
Cyprinidae , Cipriniformes , Doenças dos Peixes , Mesomycetozoea , Animais , Ecossistema
5.
Chemosphere ; 288(Pt 1): 132482, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-34627815

RESUMO

Following the Deepwater Horizon oil spill in April 2010, much research has been conducted on the cardiotoxic effects of oil on fish. Sensitive life history stages, such as the embryonic period, have been targeted to elucidate the effects of polycyclic aromatic hydrocarbons (PAHs) on the developing cardiovascular systems of fish. However, much of this research has focused on rapidly developing pelagic species, with little emphasis on estuarine species with longer embryological periods. Moreover, previous studies have used heart rate as the primary endpoint to measure cardiac performance in embryos and larvae; an endpoint that on its own may overlook impairment in cardiac performance. This study aims to fill these knowledge gaps and provide a more holistic approach for assessing the effects of PAHs on cardiac function by exposing sheepshead minnow (Cyprinodon variegatus) embryos to two oil doses (150 and 300 µg/L tPAH nominally) throughout embryonic development and measuring cardiac responses through the identification of cardiotoxic phenotypes (pericardial edema) as well as calculation of cardiac output at 4 days post fertilization. Results of this study show significant increases in pericardial edema at both oil doses relative to controls as well as significantly reduced cardiac output - driven by reductions in ventricular stroke volume. This study is one of the first to assess cardiac output in embryonic fish exposed to oil and methods described here allow for more physiologically relevant measures of cardiac performance in early life stages through established and non-invasive measures.


Assuntos
Cyprinidae , Peixes Listrados , Poluição por Petróleo , Petróleo , Hidrocarbonetos Policíclicos Aromáticos , Poluentes Químicos da Água , Animais , Petróleo/toxicidade , Poluição por Petróleo/efeitos adversos , Hidrocarbonetos Policíclicos Aromáticos/toxicidade , Poluentes Químicos da Água/toxicidade
6.
Sci Total Environ ; 806(Pt 4): 150893, 2022 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-34653448

RESUMO

Manufactured silica nanoparticles are used worldwide in large volumes for a variety of applications. An exposure of environmental organisms is therefore likely, and several data on the ecotoxicology of silica nanoparticles to different organisms have been published in recent years. This systematic review compiles and assesses these studies, in order to analyse the sensitivity distribution across different organisms. On this basis, maximum acceptable environmental concentrations are suggested and potential environmental risks are discussed. 1429 papers were retrieved from the scientific literature (Scopus), the U.S. ECOTOX knowledge database. 63 studies were finally included in the review and appraised according to the nanoCRED criteria. A total of 219 ecotoxicological endpoints recorded in 38 species (7 taxonomic groups) were condensed into a species sensitivity distribution. The resulting concentration that is hazardous for a maximum of 5% of exposed species (HC05) is 130 µg/L, from which a PNEC of 30 µg/L is estimated by applying an assessment factor of 5. These concentrations are 1-3 orders of magnitudes above the concentrations modelled to occur in European aquatic ecosystems. Algae and bacteria have a comparatively low sensitivity to MSNP exposure, likely because their cell wall forms a protective barrier against nanoparticle exposure. Similarly, embryonic stages of fish also show a comparatively low sensitivity due to the protection from their chorion. However, the fish species Labeo rohita and Oncorhynchus mykiss are among the most sensitive species. The ecotoxicity of silica nanoparticles is linked to the number of hydroxyl groups on their surface, corresponding to findings from human toxicological studies. It is recommended that future ecotoxicological studies use explicit concentration-response designs, use proven biocide-free testing material, comparatively apply mass and surface area as exposure metrics, and provide important metainformation in the study report.


Assuntos
Cyprinidae , Nanopartículas , Poluentes Químicos da Água , Animais , Organismos Aquáticos , Ecossistema , Ecotoxicologia , Humanos , Nanopartículas/toxicidade , Medição de Risco , Dióxido de Silício/toxicidade , Poluentes Químicos da Água/toxicidade
7.
Sci Total Environ ; 806(Pt 4): 150920, 2022 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-34653458

RESUMO

The toxicity of single pesticides is likely underestimated when considering complex pesticide mixtures found in agricultural runoff and this is especially true for newer pesticides with little toxicity data on non-target species. The goal of our study was to compare the toxicity of two newer pesticides, imidacloprid (IMI) and chlorantraniliprole (CHL), when an invertebrate and fish were exposed to single compounds, binary mixtures or surface water collected near agricultural fields. A secondary goal was to determine whether changes in select subcellular molecular pathways correspond to the insecticides' mechanisms of activity in aquatic organisms. We conducted acute (96 h) exposures using a dilution series of field water and environmentally relevant concentrations of single and binary mixtures of IMI and CHL. We then evaluated survival, gene expression and the activity of IMI toward the n-acetylcholine receptor (nAChR) and CHL activity toward the ryanodine receptor (RyR). Both IMI and CHL were detected at all sampling locations for May 2019 and September 2019 sampling dates and exposure to field water led to high invertebrate but not fish mortality. Fish exposed to field collected water had significant changes in the relative expression of genes involved with detoxification and neuromuscular function. Exposure of fish to single compounds or binary mixtures of IMI and CHL led to increased relative gene expression of RyR in fish. Furthermore, we found that IMI targets the nAChR in aquatic invertebrates and that CHL can cause overactivation of the RyR in invertebrates and fish. Overall, our finding suggests that IMI and CHL may impact neuromuscular health in fish. Expanding monitoring efforts to include sublethal and molecular assays would allow the detection of subcellular level effects due to complex mixtures present in surface water near agricultural areas.


Assuntos
Cyprinidae , Inseticidas , Poluentes Químicos da Água , Animais , Cyprinidae/genética , Expressão Gênica , Inseticidas/análise , Inseticidas/toxicidade , Neonicotinoides/toxicidade , Nitrocompostos/toxicidade , Água , Poluentes Químicos da Água/análise , Poluentes Químicos da Água/toxicidade , ortoaminobenzoatos
8.
Sci Total Environ ; 806(Pt 3): 151252, 2022 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-34710409

RESUMO

The temperature of the rivers in the Qilian Mountains, China varies widely from day to night, and Gymnocypris chilianensis living in these rivers may experience a change of 10 °C to 20 °C within a day. To explore the mechanisms underlying G. chilianensis responses to heat stress, we conducted an acute temperature stress experiment. In response to heat stress, levels of antioxidant enzymes (SOD\CAT\MDA) first increased and then decreased with time, but T-AOC levels only decreased. The activities of key glycolytic enzymes HK and PFK in the liver also first increased and then decreased and transaminase (AST/ALT) activity increased significantly. We obtained 5350 significantly different genes through transcriptome sequencing with enrichment pathways including primarily glycine, serine and threonine metabolism, cysteine and methionine metabolism, tryptophan metabolism, fructose and mannose metabolism, steroid hormone biosynthesis, and fatty acid degradation. A total of 457 differential metabolites were identified in the liver under thermal stress, most of which are involved in biochemical pathways of amino acid metabolism. Biosynthesis of amino acids indicated that G. chilianensis maintained physiological homeostasis by enhancing glucose metabolism and regulating lipid and amino acid metabolism pathways under thermal stress. We also randomly selected 12 key response genes for validation using qRT-PCR. This is the first study describing the mechanisms underlying responses to thermal stress in G. chilianensis, and may also provide reference data for the study of environmental mutations in indigenous fish in the Qinghai-Tibet Plateau and Qilian Mountains.


Assuntos
Cyprinidae , Transtornos de Estresse por Calor , Animais , Antioxidantes/metabolismo , Cyprinidae/metabolismo , Metabolismo Energético , Resposta ao Choque Térmico , Estresse Fisiológico
9.
Environ Pollut ; 295: 118667, 2022 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-34896397

RESUMO

Current approaches in chemical hazard assessment face significant challenges because they rely on live animal testing, which is time-consuming, expensive, and ethically questionable. These concerns serve as an impetus to develop new approach methodologies (NAMs) that do not rely on live animal tests. This study explored a molecular benchmark dose (BMD) approach using a 7-day embryo-larval fathead minnow (FHM) assay to derive transcriptomic points-of-departure (tPODs) to predict apical BMDs of fluoxetine (FLX), a highly prescribed and potent selective serotonin reuptake inhibitor frequently detected in surface waters. Fertilized FHM embryos were exposed to graded concentrations of FLX (confirmed at < LOD, 0.19, 0.74, 3.38, 10.2, 47.5 µg/L) for 32 days. Subsets of fish were subjected to omics and locomotor analyses at 7 days post-fertilization (dpf) and to histological and biometric measurements at 32 dpf. Enrichment analyses of transcriptomics and proteomics data revealed significant perturbations in gene sets associated with serotonergic and axonal functions. BMD analysis resulted in tPOD values of 0.56 µg/L (median of the 20 most sensitive gene-level BMDs), 5.0 µg/L (tenth percentile of all gene-level BMDs), 7.51 µg/L (mode of the first peak of all gene-level BMDs), and 5.66 µg/L (pathway-level BMD). These tPODs were protective of locomotor and reduced body weight effects (LOEC of 10.2 µg/L) observed in this study and were reflective of chronic apical BMDs of FLX reported in the literature. Furthermore, the distribution of gene-level BMDs followed a bimodal pattern, revealing disruption of sensitive neurotoxic pathways at low concentrations and metabolic pathway perturbations at higher concentrations. This is one of the first studies to derive protective tPODs for FLX using a short-term embryo assay at a life stage not considered to be a live animal under current legislations.


Assuntos
Cyprinidae , Poluentes Químicos da Água , Animais , Cyprinidae/genética , Fluoxetina/toxicidade , Larva , Transcriptoma
10.
Korean J Parasitol ; 59(5): 531-536, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34724775

RESUMO

The prevalence and intensity of Opisthorchis viverrini metacercariae (OvMc) were investigated in fish from 3 southern administrative regions along the Mekong River in Cambodia, i.e., Phnom Penh, Takeo, and Kandal Provinces from 2017 to 2020. A total of 295 freshwater fish (24 species) were transported to our laboratory with ice and examined using the artificial digestion method. In Phnom Penh, among 4 fish species positive for OvMc, 9 (23.7%) of 38 specimens examined were infected, and their intensity of infection averaged 4.3 metacercariae per infected fish. In Takeo Province, among 10 fish species positive for OvMc, 24 (38.1%) out of 63 fish examined were infected, and their intensity of infection was av. 14.4 metacercariae per infected fish. In particular, all of 3 Osteochilus schlegelii fish examined were infected, and their infection intensity was high, 34.7 metacercariae per fish. In Kandal Province, among 6 fish species positive for OvMc, 46 (90.2%) out of 51 specimens examined were infected, and their infection intensity was 24.0 metacercaraie per infected fish. All fish of Systomus orphoides (n=17), Barbonymus altus (n=14), and Rasbora aurotaenia (n=2) were infected, and their intensity of infection averaged 37.7, 21.6, and 18.5 metacercariae per fish, respectively. Metacercariae of Haplochis yokogawai, Haplorchis taichui, and Centrocestus formosanus were detected in fish from Takeo and Kandal Provinces. From these results, it has been confirmed that a variety of fish species from Phnom Penh, Takeo, and Kandal Provinces are commonly infected with OvMc, and preventive measures to avoid human O. viverrini infection should be performed in Cambodia.


Assuntos
Cyprinidae , Doenças dos Peixes , Opisthorchis , Infecções por Trematódeos , Animais , Camboja/epidemiologia , Cyprinidae/parasitologia , Doenças dos Peixes/epidemiologia , Doenças dos Peixes/parasitologia , Metacercárias , Prevalência , Infecções por Trematódeos/epidemiologia , Infecções por Trematódeos/veterinária
11.
Braz J Biol ; 84: e253613, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34730702

RESUMO

Soybean meal is an inexpensive plant origin protein which has been used in practical diets as a replacement of animal protein such as fish meal or chicken meal, due to the uneconomical price of animal protein diets. Consequently, a research study was conducted on some commercial species of Indian major carps i.e. Catla (Cattla cattla), Rohu (Labeo rohita) and Mrigala (Cirhinus mrigala) (Hamilton, 1822) to estimate optimum dietary protein requirement of soy bean meal in diet in an intensive polyculture. Three different diets (SBM I, SBM II and SBM III) were formulated by 80%, 50% and 20% replacement of fish meal with soybean meal from a 45% fish meal diet (control).Highest monthly mean weight gain was obtained by SBM II (with 35% CP and about 50% substitution of fish meal), while SBM III (45% Crude Protein and about 20% substitution of fish meal) was stood second. All tested diets respond enormously by producing high yield as compare to control diet, though SBM II generated highest yield among all. On the bases of the following research, it was revealed that the SBM can surrogate even50% fish meal without any augmentation of other amino acids in the diet of Indian major carps.


Assuntos
Carpas , Cyprinidae , Ração Animal/análise , Fenômenos Fisiológicos da Nutrição Animal , Animais , Dieta/veterinária , Alimentos Marinhos , Soja
12.
Braz J Biol ; 82: e242897, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34816975

RESUMO

Cyprinus carpio is the member of family cyprinidae commonly called common carp. This study was aimed to find out the comparison of brain of wild (river system) and captive (hatchery reared) population of common carp. A total of thirty samples (15 from hatchery and 15 from river Swat) were collected. All the specimens were examined in Laboratory of Parasitoloy, Zoology Department, University of Malakand. Findings indicated that wild population were greater in brain size and weight as compared to hatchery reared population. The fish samples collected from captive environment (hatchery) were showing more weight and length as compared to wild population of common carps. The mean value of total weight of hatchery fishes 345±48.68 and the mean value of brain weight of hatchery reared fishes 0.28±0.047. The mean value of wild fish's total body weight 195.16±52.58 and the mean value of brain weight of wild fishes are 0.45±0.14. Present research calls for the fact that fish in dependent environmental conditions possess brain larger in size as compared to its captive population, it is due to use and disuse of brain in their environmental requirements.


Assuntos
Carpas , Cyprinidae , Animais , Encéfalo , Rios
13.
Braz J Biol ; 83: e242922, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34817020

RESUMO

Seven hundred and twenty four fish specimens were captured from March to September 2016. The materials used in the current study were cast nets, hand nets. Eight cyprinid fish species were studied for their length-weight relationships. Parameter b in the LWR was 3.03, 3.06, 3.02, 2.29, 2.82, 3.43, 2.73 and 2.47 for Schizothorax plagiostomus, Schizothorax esocinus, Racoma labiata, Tor putitora, Barilius vagra, Garra gotyla, Puntius ticto and Arassius auratus respectively. Current study is the first attempt on the LWRs of cyprinid species, provide a baseline approach for conservation and /management of local fish fauna of economic importance.


Assuntos
Cyprinidae , Rios , Animais , Paquistão
14.
Zootaxa ; 4999(4): 389-396, 2021 Jul 13.
Artigo em Inglês | MEDLINE | ID: mdl-34810475

RESUMO

Alburnus battalgilae zulu Freyhof, 2007 from the Gediz River drainage in western Anatolia is a junior synonym of A. attalus zulu Freyhof, 2007, from the adjacent Bakr River drainage. We were unable to confirm the morphological differences originally proposed. As both species have been described in the same publication, as First Reviewers, we prioritise of A. attalus over A. battalgilae.


Assuntos
Cyprinidae , Animais , Rios
15.
Zootaxa ; 5048(2): 265-278, 2021 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-34810802

RESUMO

Recognizing and defining a species has been a controversial concern for a long time. To define the variation and the limitation between different species, especially closely related taxa in a complex species group, several concepts have been proposed which may lead to different taxonomic decisions. When a taxonomist studies a specific taxon, she/he should adopt a species concept and provide a species limitation to define the studied taxa. Garra population from the Kol River drainage, Persian Gulf basin has already been considered as Garra sp., based on molecular data, and to date no taxonomic decision has been made to provide a specific name for it. The Kol population presents several morphological characters that distinguish it from congeners: fully scaled breast; 78 branched dorsal-fin rays; caudal fin with 1617 branched rays and well-developed mental disc with free lateral and posterior margins. It is also distinguished from all other congeners in the Garra rufa group in Iran, by having two fixed, diagnostic nucleotide substitutions in the mtDNA COI barcode region. Furthermore, the Kol population demonstrates some distinct osteological characteristics in comparison to its closest species G. mondica. Based on the integrative molecular phylogenetic and species delimitation analyses, and morphological, osteological and distribution range data presented here, we think that the Kol River population merits formal description and can be considered as a distinct taxonomic unit (species).


Assuntos
Cyprinidae , Rios , Animais , Cyprinidae/genética , DNA Mitocondrial/genética , Feminino , Oceano Índico , Filogenia
16.
Zootaxa ; 5052(3): 380-394, 2021 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-34810867

RESUMO

Garra hormuzensis, new species, is described from the Kol River drainage. It is distinguished from its congeners in the Iranian part of the Persian Gulf basin by having 7 branched dorsal-fin rays, usually 9+8 branched caudal-fin rays, the breast, belly and back in front of the dorsal-fin origin covered by scales, and a free lateral and posterior margins of the gular disc. It is further characterised by having a minimum K2P distance of 1.16% to G. mondica in the mtDNA COI barcode region.


Assuntos
Cyprinidae , Rios , Animais , Cyprinidae/genética , DNA Mitocondrial , Oceano Índico
17.
Aquat Toxicol ; 241: 105999, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34678657

RESUMO

Bisphenol A (BPA) is a well-known plasticizer that widely distributed in the aquatic environment. BPA has many adverse effects on reproduction. However, few studies have investigated the mechanism of BPA affecting reproduction from the perspective of lipid metabolism. Apolipoprotein A1 (ApoA1) is the major component of high-density lipoprotein (HDL), and plays critical roles in reverse cholesterol transport (RCT). In this study, in order to investigate the effect and molecular mechanism of BPA on testicular ApoA1 and the role of ApoA1 in BPA induced abnormal spermatogenesis, adult male rare minnow Gobiocypris rarus were exposed to 15 µg/L of BPA for 1, 3 and 5 weeks. Results showed that BPA could significantly affect testicular ApoA1 mRNA and protein levels, testicular cholesterol levels, plasmatic sex hormone levels and the integrity of sperm head membrane. The main mechanism of BPA regulating ApoA1 expression is to alter Esr recruitment and CpG sites DNA methylation in ApoA1 promoter. The induced ApoA1 up-regulated high density lipoprotein cholesterol levels and enhanced RCT, and finally decreased the testicular free cholesterol levels. This is likely a key mechanism by which BPA induces sex hormone disorder and sperm head membrane damage. The present study reveals the mechanism by which BPA interferes with spermatogenesis from the perspective of cholesterol transport.


Assuntos
Cyprinidae , Poluentes Químicos da Água , Animais , Apolipoproteína A-I/genética , Compostos Benzidrílicos/toxicidade , Colesterol , Cyprinidae/genética , DNA , Masculino , Fenóis , Receptores de Estrogênio , Testículo , Poluentes Químicos da Água/toxicidade
18.
Ecotoxicol Environ Saf ; 227: 112944, 2021 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-34715502

RESUMO

Traces of psychoactive substances have been found in freshwaters globally. Fish are chronically exposed to pollution at low concentrations. The changes of aggressive behaviour of chub (Squalius cephalus) were determined under the exposure to four psychoactive compounds (sertraline, citalopram, tramadol, methamphetamine) at environmentally relevant concentrations of 1 µg/L for 42 days. We tested whether (A) the behavioural effect of compounds varies within a single species; (B) there is a correlation between the individual brain concentration of the tested pollutants and fish aggression using the novel analysis of pollutants in brain; and (C) there is detectable threshold to effective pollutant concentration in brain. Behaviour and pollutant concentrations in brain were determined repeatedly (1st, 7th, 21st, 42nd and 56th days), including a two-week-long depuration period. The effect of particular compounds varied. Citalopram and methamphetamine generally increased the fish aggression, while no such effect was found after exposure to tramadol or sertraline. The longitudinal analysis showed an aggression increase after depuration, indicating the presence of withdrawal effects in methamphetamine- and tramadol-exposed fish. The analysis of pollutant concentration in brain revealed a positive linear relationship of citalopram concentration and aggression, while no such effect was detected for other compounds and/or their metabolites. Structural break analyses detected concentration thresholds of citalopram (1 and 3 ng/g) and sertraline (1000 ng/g) in brain tissue, from which a significant effect on behaviour was manifested. While the effect of sertraline was not detected using traditional approaches, there was a reduction in aggression after considering its threshold concentration in the brain. Our results suggest that pursuing the concentration threshold of psychoactive compounds can help to reduce false negative results and provide more realistic predictions on behavioural outcomes in freshwater environments, especially in the case of compounds with bioaccumulation potential such as sertraline.


Assuntos
Cyprinidae , Preparações Farmacêuticas , Poluentes Químicos da Água , Agressão , Animais , Água Doce , Poluentes Químicos da Água/toxicidade
19.
Environ Monit Assess ; 193(11): 755, 2021 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-34716518

RESUMO

The present study aims to comparatively examine the physical quality parameters of water samples taken from Karabel, Çalti, and Tohma brooks in Sivas province and the blood biochemical parameters of blood samples of Barbus plebejus fish obtained from these waters. In periods when chemical pollution in water increased and decreased, it was determined that GLU and UA among blood biochemical parameters were significantly affected. Moreover, the potential risk levels of lead (Pb), copper (Cu), cadmium (Cd), and ferrous (Fe) for human health were compared to the international standards. Metal (Fe, Pb, Cu, and Cd) concentrations in the water were determined using atomic absorption spectrophotometer. The highest HQingestion values of Cd were found in Brook Çalti, which were 0.0018 for adults and 0.1980 for children. THQ upper limit set by the United States Environment Protection Agency (USEPA) is < 1. It was determined that HQingestion, HQdermal, and THQ values of all the heavy metals were much lower than this limit. It was concluded that water quality parameters of samples taken from Karabel, Çalti, and Tohma brooks on monthly basis for 12 months were not higher than the limits and the water qualities of brooks were determined to be "good".


Assuntos
Cyprinidae , Metais Pesados , Poluentes Químicos da Água , Adulto , Animais , Criança , Monitoramento Ambiental , Humanos , Metais Pesados/análise , Medição de Risco , Poluentes Químicos da Água/análise , Qualidade da Água
20.
BMC Ecol Evol ; 21(1): 187, 2021 10 11.
Artigo em Inglês | MEDLINE | ID: mdl-34635051

RESUMO

BACKGROUND: Individuals can estimate risk by integrating prenatal with postnatal and personal information, but the relative importance of different information sources during the transgenerational response is unclear. The estimated level of risk can be tested using the cognitive rule of risk allocation, which postulates that under consistent high-risk, antipredator efforts should decrease so that individual metabolic requirements can be satisfied. Here we conduct a comprehensive study on transgenerational risk transmission by testing whether risk allocation occurs across 12 treatments that consist of different maternal, paternal, parental care (including cross-fostering) and offspring risk environment combinations in the fathead minnow Pimephales promelas, a small cyprinid fish with alloparental care. In each risk environment, we manipulated perceived risk by continuously exposing individuals from birth onwards to conspecific alarm cues or a control water treatment. Using 2810 1-month old individuals, we then estimated shoaling behaviour prior to and subsequent to a novel mechanical predator disturbance. RESULTS: Overall, shoals estimating risk to be high were denser during the prestimulus period, and, following the risk allocation hypothesis, resumed normal shoaling densities faster following the disturbance. Treatments involving parental care consistently induced densest shoals and greatest levels of risk allocation. Although prenatal risk environments did not relate to paternal care intensity, greater care intensity induced more risk allocation when parents provided care for their own offspring as opposed to those that cross-fostered fry. In the absence of care, parental effects on shoaling density were relatively weak and personal environments modulated risk allocation only when parental risk was low. CONCLUSIONS: Our study highlights the high relative importance of parental care as opposed to other information sources, and its function as a mechanism underlying transgenerational risk transmission.


Assuntos
Cyprinidae , Animais , Sinais (Psicologia) , Células Germinativas , Humanos , Herança Paterna
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