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1.
Spectrochim Acta A Mol Biomol Spectrosc ; 267(Pt 1): 120563, 2022 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-34749113

RESUMO

A method for a sensitive fluorescence detection of glutathione was established. Glutathione-stabilized copper nanoclusters (CuNCs) were synthesized via a facile process. These CuNCs showed blue fluorescence with a peak around 450 nm. In the presence of p-benzoquinone (PBQ), the electron transfer from the copper nanoclusters to PBQ quenched the fluorescence of the CuNCs. Glutathione (GSH), as a reducing agent, formed a complex with PBQ. This formation inhibited the quenching from PBQ, and a restored fluorescence was obtained. This interaction provided a fluorescence enhancement for the measurement of GSH. Under the optimal condition, linear responses were obtained toward GSH in the ranges of 0.06-6.0 µM, with a limit of detection at 20 nM. This developed assay was easy in operation with high sensitivity and selectivity. The applicability was approved with successful glutathione measurements in real samples.


Assuntos
Cobre , Nanopartículas Metálicas , Bioensaio , Glutationa , Limite de Detecção , Espectrometria de Fluorescência
2.
Talanta ; 238(Pt 2): 123041, 2022 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-34801898

RESUMO

A novel [Co(L)(H2O)2] (1) was obtained by hydrothermal method and it exhibited a 1D chain with exposed carboxyl groups, the unique coordination mode made it have unusual physical and chemical stability. Meanwhile, 1 showed peroxidase-like and weak oxidase-like activity. 1 as a peroxidase mimic enzyme had an excellent affinity for the substrates luminol and H2O2. Compared with HRP, 1 had catalytic activity in a wide pH range and showed the best catalytic activity at pH 7.4. Meanwhile, the catalysis process of 1 was reversible and recyclable, and the catalytic activity remained stable after different pH and temperatures and long-time storage. Based on the inhibition of glutathione on luminol-H2O2-MOF 1 chemiluminescence signal, a chemiluminescence method for the determination of glutathione has been proposed with high sensitivity and selectivity and had been applied for detecting glutathione in cell lysate with satisfactory results.


Assuntos
Estruturas Metalorgânicas , Catálise , Glutationa , Peróxido de Hidrogênio , Limite de Detecção , Luminescência , Medições Luminescentes , Luminol
3.
Biol Trace Elem Res ; 200(1): 147-155, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33689144

RESUMO

Hypercapnia can increase the production of reactive oxygen species (ROS) by inducing oxidative stress in cells. Transient receptor potential melastatin 2 (TRPM2) channel activation that is realized by ROS plays a critical role in the cellular mechanism. It was shown that antioxidants such as zinc (Zn), selenium (Se), and glutathione (GSH) can partake in the structures of enzymes and create a protective effect against oxidative stress. This study revealed the relationship between TRPM2 channel and hypercapnia, and the interaction of zinc, selenium, and glutathione. In our study, normoxia, hypercapnia, hypercapnia + Zn, hypercapnia + Se, and hypercapnia + GSH were created, in transfected HEK293 cells. The cells were exposed to normoxia or hypercapnia gasses in two different times (30 min and 60 min), while Zn, Se, and GSH were applied to the cells in the other groups before being exposed to the gas mixtures. The statistical evaluation showed a significant increase in lipid peroxidation (LPO) level and lactate dehydrogenase (LDH)% in the hypercapnia 30 min and 60 min groups, compared to the normoxia 30 min and 60 min groups, and an increase in LPO level and LDH% in the hypercapnia groups that Zn, Se, and GSH were applied. It was determined that in comparison with the normoxia 30 min and 60 min groups, the amount of inward Ca+2 current across TRPM2 channels and mean current density increased in the groups that were exposed to hypercapnia for 30 min and 60 min, while the same values significantly decreased in the hypercapnia groups that Zn, Se, and GSH were applied. Also, it was shown that oxidative stress rose as the duration of hypercapnia exposure increased. It was concluded that hypercapnia increased oxidative stress and caused cellular membrane damage, while the addition of Zn, Se, and GSH could protect the cell membrane from these damaging effects.


Assuntos
Acidose , Selênio , Canais de Cátion TRPM , Glutationa/metabolismo , Células HEK293 , Humanos , Zinco
4.
J Cell Biol ; 221(2)2022 02 07.
Artigo em Inglês | MEDLINE | ID: mdl-34817556

RESUMO

Ferroptosis is an oxidative and iron-dependent form of regulated cell death (RCD) recently described in eukaryotic organisms like animals, plants, and parasites. Here, we report that a similar process takes place in the photosynthetic prokaryote Synechocystis sp. PCC 6803 in response to heat stress. After a heat shock, Synechocystis sp. PCC 6803 cells undergo a cell death pathway that can be suppressed by the canonical ferroptosis inhibitors, CPX, vitamin E, Fer-1, liproxstatin-1, glutathione (GSH), or ascorbic acid (AsA). Moreover, as described for eukaryotic ferroptosis, this pathway is characterized by an early depletion of the antioxidants GSH and AsA, and by lipid peroxidation. These results indicate that all of the hallmarks described for eukaryotic ferroptosis are conserved in photosynthetic prokaryotes and suggest that ferroptosis might be an ancient cell death program.


Assuntos
Cianobactérias/citologia , Cianobactérias/metabolismo , Ferroptose , Ferro/metabolismo , Antioxidantes/metabolismo , Ácido Ascórbico/metabolismo , Cálcio/metabolismo , Caspase 3/metabolismo , Caspase 7/metabolismo , Citosol/metabolismo , Glutationa/metabolismo , Resposta ao Choque Térmico , Lipidômica , Lipídeos/química , Oxirredução , Espécies Reativas de Oxigênio/metabolismo , Synechocystis/metabolismo , Tilacoides/metabolismo
5.
J Hazard Mater ; 424(Pt A): 127310, 2022 02 15.
Artigo em Inglês | MEDLINE | ID: mdl-34879548

RESUMO

The current study was designed to assess nanomaterial sulfonated graphene oxide (SGO) potential in improving tolerance of wheat chloroplasts against nitrate (NS) and ammonium (AS) toxicity. Triticum aestivum cv. Ekiz was grown under SGOs (50-250-500 mg L-1) with/without 140 mM NS and 5 mM AS stress. SGOs were eliminated the adverse effects produced by stress on chlorophyll fluorescence, potential photochemical efficiency and physiological state of the photosynthetic apparatus. SGO reversed the negative effects on these parameters. Upon SGOs exposure, the induced expression levels of photosystems-related reaction center proteins were observed. SGOs reverted radical accumulation triggered by NS by enabling the increased superoxide dismutase (SOD) activity and ascorbate (AsA) regeneration. Under AS, the turnover of both AsA and glutathione (GSH) was maintained by 50-250 mg L-1 SGO by increasing the enzymes and non-enzymes related to AsA-GSH cycle. 500 mg L-1 SGO prevented the radical over-accumulation produced by AS via the regeneration of AsA and peroxidase (POX) activity rather than GSH regeneration. 50-250 mg L-1 SGO protected from the NS+AS-induced disruptions through the defense pathways connected with AsA-GSH cycle represented the high rates of AsA/DHA and, GSH/GSSG and GSH redox state. Our findings specified that SGO to NS and AS-stressed wheat provides a new potential tool to advance the tolerance mechanism.


Assuntos
Compostos de Amônio , Nanoestruturas , Compostos de Amônio/metabolismo , Antioxidantes/metabolismo , Ácido Ascórbico/metabolismo , Cloroplastos/metabolismo , Glutationa/metabolismo , Grafite , Nitratos/metabolismo , Oxirredução , Triticum/metabolismo
6.
J Sci Food Agric ; 102(2): 575-583, 2022 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-34148247

RESUMO

BACKGROUND: Yeast hydrolysate (YH) has multiple salutary biological activities. Nevertheless, the application of YH in broiler production is limited. This study was conducted to evaluate the protective effects of YH derived from Saccharomyces cerevisiae by exploring growth performance, serum parameters, organs relative weight, carcass traits, meat quality and antioxidant status of broilers. RESULTS: Supplementing YH linearly and quadratically improved (P < 0.05) body weight gain and gain-to-feed ratio compared to that in the control group. Triglycerides, low-density lipoprotein cholesterol and total cholesterol in serum, the decline in pH and cooking loss of breast muscle, and malonaldehyde concentration in serum and liver were decreased linearly and/or quadratically by YH (P < 0.05), whereas high-density lipoprotein cholesterol in serum, superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities in serum, GSH-Px activity in liver, glutathione content in serum and liver, eviscerated yield rate and chest muscle yield, and the relative weight of spleen and liver were linearly and/or quadratically increased (P < 0.05). Moreover, YH enhanced the mRNA levels of nuclear factor erythroid 2-related factor 2, heme oxygennase-1 (HO-1), GSH-Px1 and SOD1 (linear and/or quadratic, P < 0.05). CONCLUSION: Dietary YH beneficially affected growth performance, serum parameters, organ relative weight, carcass traits, meat quality and antioxidant status in broilers, indicating its potential application as a promising feed additive in broiler production. © 2021 Society of Chemical Industry.


Assuntos
Antioxidantes/metabolismo , Galinhas/crescimento & desenvolvimento , Suplementos Nutricionais/análise , Carne/análise , Hidrolisados de Proteína/análise , Saccharomyces cerevisiae/química , Ração Animal/análise , Animais , Peso Corporal , Galinhas/sangue , Galinhas/metabolismo , Colesterol/sangue , Glutationa/metabolismo , Malondialdeído/metabolismo , Músculo Esquelético/química , Músculo Esquelético/metabolismo , Hidrolisados de Proteína/metabolismo , Superóxido Dismutase/metabolismo , Triglicerídeos/sangue
7.
Endocrinology ; 163(1)2022 01 01.
Artigo em Inglês | MEDLINE | ID: mdl-34647995

RESUMO

Seminal plasma contains a high concentration of extracellular vesicles (EVs). The heterogeneity of small EVs or the presence of nonvesicular extracellular matter (NV) pose major obstacles in understanding the composition and function of seminal EVs. In this study, we employed high-resolution density gradient fractionation to accurately characterize the composition and function of seminal EVs and NV. We found that the seminal EVs could be divided into 3 different subtypes-namely, high-density EV (EV-H), medium-density EV (EV-M), and low-density EV (EV-L)-after purification using iodixanol, while NV was successfully isolated. EVs and NV display different features in size, shape, and expression of some classic exosome markers. Both EV-H and NV could markedly promote sperm motility and capacitation compared with EV-M and EV-L, whereas only the NV fraction induced sperm acrosome reaction. Proteomic analysis results showed that EV-H, EV-M, EV-L, and NV had different protein components and were involved in different physiological functions. Further study showed that EV-M might reduce the production of sperm intrinsic reactive oxygen species through glutathione S-transferase mu 2. This study provides novel insights into important aspects of seminal EVs constituents and sounder footing to explore their functional properties in male fertility.


Assuntos
Vesículas Extracelulares/metabolismo , Proteômica/métodos , Sêmen/metabolismo , Motilidade Espermática , Reação Acrossômica , Biomarcadores/metabolismo , Biotinilação , Biologia Computacional , Exossomos/metabolismo , Glutationa/metabolismo , Glutationa Transferase/metabolismo , Humanos , Masculino , Fosforilação , Proteínas Tirosina Fosfatases/química , Proteoma , Espécies Reativas de Oxigênio , Espermatozoides/metabolismo , Espermatozoides/fisiologia , Ácidos Tri-Iodobenzoicos/farmacologia
8.
Sci Total Environ ; 806(Pt 2): 150703, 2022 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-34600989

RESUMO

1,4-Dioxane (DX) is a synthetic chemical used as a stabilizer for industrial solvents. Recent occurrence data show widespread and significant contamination of drinking water with DX in the US. DX is classified by the International Agency for Research on Cancer as a group 2B carcinogen with the primary target organ being the liver in animal studies. Despite the exposure and cancer risk, US EPA has not established a drinking water Maximum Contaminant Level (MCL) for DX and a wide range of drinking water targets have been established across the US and by Health Canada. The DX carcinogenic mechanism remains unknown; this information gap contributes to the varied approaches to its regulation. Our recent mice study indicated alterations in oxidative stress response accompanying DNA damage as an early change by high dose DX (5000 ppm) in drinking water. Herein, we report a follow-up study, in which we used glutathione (GSH)-deficient glutamate-cysteine ligase modifier subunit (Gclm)-null mice to investigate the role of redox homeostasis in DX-induced liver cytotoxicity and genotoxicity. Gclm-null and wild-type mice were exposed to DX for one week (1000 mg/kg/day by oral gavage) or three months (5000 ppm in drinking water). Subchronic exposure of high dose DX caused mild liver cytotoxicity. DX induced assorted molecular changes in the liver including: (i) a compensatory nuclear factor erythroid 2-related factor 2 (NRF2) anti-oxidative response at the early stage (one week), (ii) progressive CYP2E1 induction, (iii) development of oxidative stress, as evidenced by persistent NRF2 induction, oxidation of GSH pool, and accumulation of the lipid peroxidation by-product 4-hydroxynonenal, and (iv) elevations in oxidative DNA damage and DNA repair response. These DX-elicited changes were exaggerated in GSH-deficient mice. Collectively, the current study provides additional evidence linking redox dysregulation to DX liver genotoxicity, implying oxidative stress as a candidate mechanism of DX liver carcinogenicity.


Assuntos
Dano ao DNA , Estresse Oxidativo , Animais , Dioxanos , Seguimentos , Glutationa/metabolismo , Fígado/metabolismo , Camundongos , Camundongos Knockout
9.
Talanta ; 238(Pt 1): 122999, 2022 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-34857332

RESUMO

This work initially reports a new nanosening method for simple, sensitive, specific, visual detection of mercury (II) (Hg2+) and glutathione (GSH) using the Tyndall Effect (TE) of the same colloidal gold nanoparticle (GNP) probes for efficient colorimetric signaling amplification. For the TE-inspired assay (TEA) method, arginine (Arg) molecules are pre-modified on the GNPs' surfaces (Arg-GNPs). Upon the Hg2+ introduction, it can be specifically coordinated with the terminal -NH2 and -COOH groups of the Arg molecules to make the Arg-GNPs aggregate, producing a significantly-enhanced TE signal in the reaction solution after its irradiation by a 635-nm red laser pointer pen. On the other hand, the introduction of the GSH results in the production of the original Arg-GNPs' weak TE response, as it is able to bind such metal ion via mercury-thiol reactions to inhibit the above aggregation. Under the optimal conditions, the utility of the new TEA method is well demonstrated to quantitatively detect the Hg2+ and GSH with the aid of a smartphone as a portable TE reader during the linear concentration ranges of 50-3000 and 10-3000 nM, respectively. The detection limits for the Hg2+ and GSH are estimated to be as low as ∼3.5 and ∼0.3 nM, respectively. The recovery results obtained from the detection of Hg2+ in the complex tap and pond water samples and the assay of GSH in real human serum and urine samples are also satisfactory.


Assuntos
Mercúrio , Nanopartículas Metálicas , Colorimetria , Glutationa , Ouro , Humanos , Íons
10.
Ecotoxicol Environ Saf ; 229: 113078, 2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-34929502

RESUMO

Leptodictyum riparium, a widely distributed aquatic moss, can both tolerate and accumulate very high concentrations of toxic heavy metals, with only slight apparent damage. Here we report the effects on photosynthetic yield, glutathione (GSH), phytochelatin (PCn) synthesis, nitrogen metabolism and cellular localization of molecules rich in SH groups in L. riparium exposed in vitro to heavy metals. We simulated the concentrations of Cu, Zn, Cd, Pb detected in Regi Lagni, Italy, one of the most contaminated freshwater sites in Southern Europe, in the laboratory to test how the moss responds to heavy metal contamination. There was a steady decrease of photosynthetic efficiency correlated with the heavy metal concentrations and ultrastructural organization. All PCn levels increased significantly as the concentration of heavy metals increased, while the GSH levels did not appear to be particularly affected. A significant increase of GDH and NADH-GOGAT activities increased with increasing heavy metal concentration. Immunoblotting analysis revealed an increase of the chl-GS2 while no significant increase was detected in the cyt-GS1. These results give insight into the molecular events underlying the metal-tolerance of the aquatic moss L. riparium exposed to environmental heavy metal concentrations.


Assuntos
Briófitas , Bryopsida , Metais Pesados , Monitoramento Ambiental , Água Doce , Glutationa , Metais Pesados/análise , Metais Pesados/toxicidade
11.
Ecotoxicol Environ Saf ; 229: 113111, 2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-34952378

RESUMO

Acrolein (ACR) is a metabolic byproduct in vivo and a ubiquitous environmental toxicant. It is implicated in the initiation and development of many diseases through multiple mechanisms, including the induction of oxidative stress. Currently, our understanding of the body defense mechanism against ACR toxicity is still limited. Given that hydrogen sulfide (H2S) has strong antioxidative actions and it shares several properties of ACR scavenger glutathione (GSH), we, therefore, tested whether H2S could be involved in ACR detoxification. Taking advantage of two cell lines that produced different levels of endogenous H2S, we found that the severity of ACR toxicity was reversely correlated with H2S-producing ability. In further support of the role of H2S, supplementing cells with exogenous H2S increased cell resistance to ACR, whereas inhibition of endogenous H2S sensitized cells to ACR. In vivo experiments showed that inhibition of endogenous H2S with CSE inhibitor markedly increased mouse susceptibility to the toxicity of cyclophosphamide and ACR, as evidenced by the increased mortality and worsened organ injury. Further analysis revealed that H2S directly reacted with ACR. It promoted ACR clearance and prevented ACR-initiated protein carbonylation. Collectively, this study characterized H2S as a presently unrecognized endogenous scavenger of ACR and suggested that H2S can be exploited to prevent and treat ACR-associated diseases.


Assuntos
Sulfeto de Hidrogênio , Acroleína/toxicidade , Animais , Antioxidantes , Glutationa/metabolismo , Sulfeto de Hidrogênio/toxicidade , Camundongos , Estresse Oxidativo
12.
Methods Mol Biol ; 2363: 199-213, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-34545495

RESUMO

Mitochondria actively participate in oxygenic metabolism and are one of the major sources of reactive oxygen species (ROS) production in plant cells. However, instead of measuring ROS concentrations in organelles it is more worthwhile to observe active ROS generation or downstream oxidation products, because the steady state level of ROS is easily buffered. Here, we describe how to measure the in vitro production of superoxide anion radicals (O2·-) by mitochondria and the release of O2·- into the cytosol. A method to determine glutathione, which is the most abundant mitochondrial low-mass antioxidant, is presented since changes in the redox state of glutathione can be indicative of the oxidative action of ROS. The identification of oxidative damage to mitochondrial components is the ultimate symptom that ROS homeostasis is not under control. We present how to determine the extent of oxidation of membrane lipids and the carbonylation of mitochondrial proteins. In summary, oxidative stress symptoms have to be analyzed at different levels, including ROS production, scavenging capacity, and signs of destruction, which only together can be considered markers of mitochondrial ROS status.


Assuntos
Mitocôndrias , Estresse Oxidativo , Glutationa/metabolismo , Mitocôndrias/metabolismo , Proteínas Mitocondriais/metabolismo , Oxirredução , Espécies Reativas de Oxigênio/metabolismo
13.
Anal Biochem ; 637: 114475, 2022 01 15.
Artigo em Inglês | MEDLINE | ID: mdl-34813770

RESUMO

In this study, we developed a novel colorimetric chemosensor for selective and sensitive recognition of Glutathione (GSH) using a simple binary mixture of commercially accessible and inexpensive metal receptors with names, Bromo Pyrogallol Red (BPR) and Xylenol Orange (XO). This procedure is based on the synergistic coordination of BPR and XO with cerium ion (Ce3+) for the recognition of GSH over other available competitive amino acids (AAs) especially thiol species in aqueous media. Generally, cysteine (Cys) and homocysteine (hCys) can seriously interfere with the detection of GSH among common biological species because they possess similar chemical behavior. Using all the information from 1HNMR and FT-IR studies, the proposed interaction is presented in which GSH acts as a tri-dentate ligand with three N donor atoms in conjunction with BPR and XO as mono and bi-dentate ligands respectively. This approach opens a path for selective detection of other AAs by argumentatively selecting the ensemble of mixed organic ligands from commercially available reagents, thereby eliminating the need for developing synthetic receptors, sample preparation, organic solvent mixtures, and expensive equipment. Evaluating the feasibility of the existing method was led to the determination of GSH in human plasma samples.


Assuntos
Cério/química , Colorimetria/métodos , Corantes/química , Glutationa/sangue , Fenóis/química , Pirogalol/análogos & derivados , Sulfóxidos/química , Técnicas Biossensoriais/métodos , Cisteína/análise , Cisteína/química , Humanos , Indicadores e Reagentes/química , Ligantes , Espectroscopia de Ressonância Magnética/métodos , Pirogalol/química , Espectroscopia de Infravermelho com Transformada de Fourier/métodos , Compostos de Sulfidrila/análise , Compostos de Sulfidrila/química , Água/química
14.
J Colloid Interface Sci ; 607(Pt 2): 1516-1526, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-34592546

RESUMO

Sorafenib-mediated chemotherapy is currently the first choice for hepatocellular carcinoma (HCC) that cannot be surgically excised, and can significantly improve the survival of patients. However, its poor water solubility restricts its bioavailability, and long-term single use of it does not achieve satisfactory HCC therapy effects. Herein, we report a novel cascaded copper-based metal-organic framework (MOF) therapeutic nanocatalyst using HKUST-1 by integrating cyclooxygenase-2 (COX-2) inhibitor meloxicam (Mel) and chemotherapeutic agent sorafenib (Sol) to amplify HCC therapy. This HKUST-1 nanocatalyst can be degraded by glutathione (GSH) into a Fenton-like agent to trigger chemodynamic therapy (CDT). CDT-mediated cytotoxic reactive oxygen species (ROS) can activate ferroptosis by accumulating lipid peroxides (LPO). Alternatively, GSH depletion not only deactivates glutathione peroxidase 4 (GPX4) to trigger ferroptosis, but also leads to oxidative stress amplification. Moreover, Sol can also activate ferroptosis by inhibiting system XC-, resulting in cascade-amplified ferroptosis mediated HCC therapy. Furthermore, the down-regulation of COX-2 can induce PINK1/Parkin-mediated mitophagy to further act synergistically with Sol-mediated chemotherapy. Therefore, this HKUST-1 nanocatalyst provides a novel strategy to regulate GSH and COX-2 levels for amplified chemo/chemodynamic and ferroptosis-mediated HCC therapy.


Assuntos
Carcinoma Hepatocelular , Neoplasias Hepáticas , Carcinoma Hepatocelular/tratamento farmacológico , Cobre , Ciclo-Oxigenase 2 , Glutationa , Humanos , Neoplasias Hepáticas/tratamento farmacológico
15.
Food Chem ; 372: 131142, 2022 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-34600193

RESUMO

To determine the glutathione (GSH) content in vegetables, an "on-off-on" fluorescence probe was developed by a synthesis of nitrogen and sulfur co-doped carbon dots (N,S-CDs) using the microwave pyrolysis considering citric acid and L-cysteine as precursors. The fluorescence of N,S-CDs was quenched by adding Cu2+ at a concentration of 20-200 µmol/L due to the inner filter effect. The quenched fluorescence of N,S-CDs@Cu2+ system was recovered by adding the GSH at a concentration of 10-150 µmol/L due to the sulfhydryl-metal compound mechanism. By observing the GSH concentrations measured by our N,S-CDs@Cu2+ system vs. a traditional fluorescent chelating method, the two measurements provided the GSH data with a good consistence by showing the RSD range of 1.86%-2.27%. This indicates the validation and novelty of our N,S-CDs@Cu2+ system as being a powerful fluorescent probe for effectively and efficiently determining the GSH in vegetables.


Assuntos
Glutationa , Pontos Quânticos , Carbono , Corantes Fluorescentes , Nitrogênio , Espectrometria de Fluorescência , Enxofre , Verduras
16.
Spectrochim Acta A Mol Biomol Spectrosc ; 266: 120409, 2022 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-34607091

RESUMO

Biothiols play an important role in many physiological and pathological processes, especially in the occurrence of oxidative stress caused by abnormal cysteine (Cys) concentration. Therefore, it is particularly critical to develop a method that can specifically identify Cys to avoid interference from other biological analytes. However, most Cys-specific fluorescent probes are difficult to distinguish between homocysteine (Hcy) and glutathione (GSH). In this work, to avoid the interference of Hcy and GSH, we developed a fluorescent probe triarylimidazole-naphthalimide-piperazine-sulfonyl benzoxadiazole (TNP-SBD-Cl) based on fluorescence resonance energy transfer (FRET) on platform of naphthalimide-sulfonyl benzoxadiazole (SBD), the main SBD 4-chlorine groups have mild reactivity to undergo substitution and rearrangement to distinguish Hcy and GSH. The TNP-SBD-Cl response to Cys would turn on FRET and generate a new yellow fluorescence with a large Stokes shift (157 nm), and with excellent selectivity and low detection limit (0.87 µM). Moreover, TNP-SBD-Cl can be used to monitor Cys in living HeLa cells with low cytotoxicity, suggesting that it has markedly diagnostic significance in physiological and pathological processes.


Assuntos
Cisteína , Corantes Fluorescentes , Glutationa , Células HeLa , Homocisteína , Humanos , Naftalimidas , Imagem Óptica , Espectrometria de Fluorescência
17.
Talanta ; 237: 122957, 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-34736682

RESUMO

The development of an efficient protein-inorganic nanohybrid with superior nanozyme activity for highly sensitive detection of glutathione (GSH) is essential for early diagnosis of human diseases. Herein, a rapid and highly sensitive colorimetric assay using self-assembled bovine serum albumin-hydrated manganese phosphate nanoflowers (MnPNF) as a biomimic oxidase is developed for GSH detection in human serum. The BSA can complex with Mn2+ to serve the nucleation center to produce MnPNF in the presence of phosphate-buffered saline (PBS). The morphology and surface characterization results show that the MnPNF is assembled with hierarchical nanoplates to form 500 nm nanoflowers. The oxidase-like activity of MnPNF is based on the redox reaction with 3,3',5,5'-tetramethylbenzidine. However, the addition of GSH can reduce MnPNF to Mn2+, and subsequently supresses the oxidase-like activity and a yellow color at 450 nm is observed in the presence of H2SO4. The MnPNF-based nanozyme exhibits excellent sensing ability toward GSH detection, and a good linear relationship between the change in absorbance at 450 nm and the added amounts of GSH at 50 nM-10 µM with low limits of detection of 20 and 26.6 nM in the PBS and diluted human serum, respectively, is observed. Moreover, the sensing probe shows a superior selectivity over the other 16 interferences, which drive the determination of GSH feasible in real human serum. Since the MnPNF can be simply prepared at room temperature and no functionalization is required, this assay can be used to design the highly efficient biomimic oxidase for effective sensing of GSH and other disease-related biomolecules in biological fluid samples.


Assuntos
Colorimetria , Glutationa , Humanos , Compostos Organometálicos , Oxirredução , Oxirredutases
18.
Biochim Biophys Acta Mol Basis Dis ; 1868(1): 166287, 2022 01 01.
Artigo em Inglês | MEDLINE | ID: mdl-34626772

RESUMO

Oxidative stress and lipid peroxidation are major causes of skin injury induced by ultraviolet (UV) irradiation. Ferroptosis is a form of regulated necrosis driven by iron-dependent peroxidation of phospholipids and contributes to kinds of tissue injuries. However, it remains unclear whether the accumulation of lipid peroxides in UV irradiation-induced skin injury could lead to ferroptosis. We generated UV irradiation-induced skin injury mice model to examine the accumulation of the lipid peroxides and iron. Lipid peroxides 4-HNE, the oxidative enzyme COX2, the oxidative DNA damage biomarker 8-OHdG, and the iron level were increased in UV irradiation-induced skin. The accumulation of iron and lipid peroxidation was also observed in UVB-irradiated epidermal keratinocytes without actual ongoing ferroptotic cell death. Ferroptosis was triggered in UV-irradiated keratinocytes stimulated with ferric ammonium citrate (FAC) to mimic the iron overload. Although GPX4 protected UVB-injured keratinocytes against ferroptotic cell death resulted from dysregulation of iron metabolism and the subsequent increase of lipid ROS, keratinocytes enduring constant UVB treatment were markedly sensitized to ferroptosis. Nicotinamide mononucleotide (NMN) which is a direct and potent NAD+ precursor supplement, rescued the imbalanced NAD+/NADH ratio, recruited the production of GSH and promoted resistance to lipid peroxidation in a GPX4-dependent manner. Taken together, our data suggest that NMN recruits GSH to enhance GPX4-mediated ferroptosis defense in UV irradiation-induced skin injury and inhibits oxidative skin damage. NMN or ferroptosis inhibitor might become promising therapeutic approaches for treating oxidative stress-induced skin diseases or disorders.


Assuntos
Glutationa/genética , Ferro/metabolismo , Estresse Oxidativo/genética , Fosfolipídeo Hidroperóxido Glutationa Peroxidase/genética , Pele/metabolismo , 8-Hidroxi-2'-Desoxiguanosina/farmacologia , Aldeídos/farmacologia , Animais , Ciclo-Oxigenase 2/genética , Dano ao DNA/efeitos dos fármacos , Dano ao DNA/efeitos da radiação , Compostos Férricos/farmacologia , Ferroptose/efeitos dos fármacos , Ferroptose/efeitos da radiação , Humanos , Queratinócitos/efeitos dos fármacos , Queratinócitos/efeitos da radiação , Peroxidação de Lipídeos/efeitos dos fármacos , Peroxidação de Lipídeos/efeitos da radiação , Peróxidos Lipídicos/farmacologia , Camundongos , Mononucleotídeo de Nicotinamida/farmacologia , Estresse Oxidativo/efeitos dos fármacos , Estresse Oxidativo/efeitos da radiação , Compostos de Amônio Quaternário/farmacologia , Pele/efeitos dos fármacos , Pele/lesões , Pele/patologia , Raios Ultravioleta/efeitos adversos
19.
J Colloid Interface Sci ; 606(Pt 1): 67-76, 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-34388574

RESUMO

Carbon dots have garnered significant attention owing to their versatile and highly tunable optical properties; however, the origins and the underlying mechanism remains a subject of debate especially for dual fluorescent systems. Here, we have prepared carbon dots from glutathione and formamide precursors via a one-pot solvothermal synthesis. Steady state and dynamic techniques indicate that these dual fluorescent dots possess distinct emissive carbon-core and a molecular states, which are responsible for the blue and red optical signatures, respectively. To further glean information into the fluorescence mechanism, electrochemical analysis was used to measure the bandgaps of the two fluorescent states, while femtosecond transient absorption spectroscopy evidenced the two-state model based on the observed heterogeneity and bimodal spectral distribution. Our findings provide novel and fundamental insights on the optical properties of dual fluorescent dots, which can translate to more effective and targeted application development particularly in bioimaging, multiplexed sensing and photocatalysis.


Assuntos
Carbono , Pontos Quânticos , Glutationa , Espectrometria de Fluorescência
20.
Spectrochim Acta A Mol Biomol Spectrosc ; 264: 120247, 2022 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-34399295

RESUMO

We developed a fluorescent probe, named 2-(4-(acryloyloxy) phenyl)-4-(2-carboxyphenyl)-7-(diethylamino) chromenylium (PA-A), for detecting Cys using the -OH protection/deprotection strategy, which can react with Cys to form a red-emitting anthocyanidin derivative fluorophore. The probe has high selectivity to Cys over Hcy and GSH in phosphate buffer solution (PBS, 10 mM, pH = 7.4), high sensitivity, a low detection limit of 4.48 × 10-8 mol/L, and it can be recognized with the naked eye. Fluorescence imaging experiment of Cys with PA-A at the cellular successfully showed excellent tissue penetration.


Assuntos
Cisteína , Corantes Fluorescentes , Glutationa , Células HeLa , Homocisteína , Humanos , Imagem Óptica
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