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1.
Dent Mater ; 38(7): 1162-1172, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-35690483

RESUMO

OBJECTIVE: To characterize the effect of elemental composition and manufacturing process on the electrochemical properties of Co-Cr-Mo, Co-Cr-W and Co-Cr-Mo-W alloys. METHODS: Six Co-Cr based alloys were included in this study. All alloys are Co-Cr based alloys, classified in three different types according to their elemental composition. The first group has Mo as the third alloying element while the second one has W instead of Mo. The third one has both alloying elements. The groups are further divided by the manufacturing process (casting or Selective Laser Melting(SLM)). All groups were subjected to static immersion, open circuit potential, anodic scan, SEM/EDX analysis, static and cyclic tarnish testing according to ISO 10271 requirements. The ionic release was evaluated by inductively coupled plasma mass spectrometry and the results were statistically analyzed by two way ANOVA and Tukey test (a=0.05). RESULTS: No statistical differences were identified for Co-Cr-Mo alloy for all elements and their total ionic release between casting and SLM manufacturing processes, in contrast to significantly lower values for SLM groups for the other two groups. All groups tested demonstrated similar performance in OCP and AS testing while no gross elemental changes before and after AS were identified following EDX analysis. All alloys fulfilled the requirements of tarnish resistance CONCLUSIONS: The ionic release is dependent on alloy type and manufacturing process while all groups were found to fulfill the requirements of international standards for ionic release, corrosion and tarnish resistance and thus an acceptable clinical performance is anticipated.


Assuntos
Ligas de Cromo , Técnica de Fundição Odontológica , Ligas , Ligas de Cromo/química , Corrosão , Ligas Dentárias/química , Lasers , Teste de Materiais , Propriedades de Superfície
2.
Transpl Int ; 35: 10455, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35711322

RESUMO

Endogenous production of carbon monoxide (CO) is affected by inflammatory phenomena and ischemia-reperfusion injury. Precise measurement of exhaled endogenous CO (eCO) is possible thanks to a laser spectrometer (ProCeas® from AP2E company). We assessed eCO levels of human lung grafts during the normothermic Ex-Vivo Lung Perfusion (EVLP). ProCeas® was connected in bypass to the ventilation circuit. The surgical team took the decision to transplant the lungs without knowing eCO values. We compared eCO between accepted and rejected grafts. EVLP parameters and recipient outcomes were also compared with eCO values. Over 7 months, eCO was analyzed in 21 consecutive EVLP grafts. Two pairs of lungs were rejected by the surgical team. In these two cases, there was a tendency for higher eCO values (0.358 ± 0.52 ppm) compared to transplanted lungs (0.240 ± 0.76 ppm). During the EVLP procedure, eCO was correlated with glucose consumption and lactate production. However, there was no association of eCO neither with edema formation nor with the PO2/FiO2 ratio per EVLP. Regarding post-operative data, every patient transplanted with grafts exhaling high eCO levels (>0.235 ppm) during EVLP presented a Primary Graft Dysfunction score of 3 within the 72 h post-transplantation. There was also a tendency for a longer stay in ICU for recipients with grafts exhaling high eCO levels during EVLP. eCO can be continuously monitored during EVLP. It could serve as an additional and early marker in the evaluation of the lung grafts providing relevant information for post-operative resuscitation care.


Assuntos
Expiração , Transplante de Pulmão , Humanos , Lasers , Pulmão , Transplante de Pulmão/métodos , Perfusão/métodos
3.
Medicine (Baltimore) ; 101(25): e29250, 2022 Jun 24.
Artigo em Inglês | MEDLINE | ID: mdl-35758354

RESUMO

RATIONALE: Femtosecond laser-assisted cataract surgery (FLACS) has grown in popularity among ophthalmologists as a novel surgical technique. However, malignant glaucoma (MG) is a complication of FLACS. Herein, we report a case of MG following FLACS. PATIENT CONCERNS: A 66-year-old woman presented with complaints of blurred vision in the right eye and a foreign body sensation in both eyes. Ophthalmological examinations showed that the corrected distance visual acuity was 20/50 and 20/25 in the right and left eyes, respectively. Without any topical anti-glaucoma medication, the intraocular pressure (IOP) was 20 mmHg in the right eye and 17 mmHg in the left eye. Slit-lamp examination of the right eye revealed a transparent cornea with a defect in the punctate overlying epithelium; the central anterior chamber depth was shallow the peripheral iris laser shot was visible, the pupil was normal, and the lens was mainly cortical opacified. DIAGNOSES: Based on the patient's symptoms, examination results, and preliminary diagnoses, age-related cataract in the right eye, binocular post-antiglaucoma surgery, pseudophakicin in the left eye, and Sjogren syndrome were included. INTERVENTIONS: FLACS was performed to facilitate anterior capsulotomy and segmentation of the nucleus in the right eye. MG occurred after the femtosecond procedure, and with the treatment of medicines combined with phacoemulsification, IOP was eventually normal without further antiglaucoma therapy. OUTCOMES: IOP was 16 mmHg on postoperative day 1. Ocular ultrasonography revealed no choroid detachment or hemorrhage in the right eye. Two weeks postoperatively, uncorrected visual acuity was 20/25, and IOP remained normal with no further antiglaucoma treatment on 1 month postoperatively. CONCLUSIONS: We describe the occurrence of MG after FLACS and illustrate that miosis and bubble formation after FLACS may be risk factors for MG during FLACS.


Assuntos
Extração de Catarata , Catarata , Glaucoma , Terapia a Laser , Facoemulsificação , Idoso , Catarata/complicações , Extração de Catarata/efeitos adversos , Extração de Catarata/métodos , Feminino , Glaucoma/complicações , Glaucoma/cirurgia , Humanos , Terapia a Laser/efeitos adversos , Terapia a Laser/métodos , Lasers , Facoemulsificação/métodos
4.
J Phys Chem Lett ; 13(24): 5413-5423, 2022 Jun 23.
Artigo em Inglês | MEDLINE | ID: mdl-35679146

RESUMO

Femtosecond laser pulses readily produce coherent quantum beats in transient-absorption spectra. These oscillatory signals often arise from molecular vibrations and therefore may contain information about the excited-state potential energy surface near the Franck-Condon region. Here, by fitting the measured spectra of two laser dyes to microscopic models of femtosecond coherence spectra (FCS) arising from molecular vibrations, we classify coherent quantum-beat signals as fundamentals or overtones and quantify their Huang-Rhys factors and anharmonicity values. We discuss the extracted Huang-Rhys factors in the context of quantum-chemical computations. This work solidifies the use of FCS for analysis of coherent quantum beats arising from molecular vibrations, which will aid studies of molecular aggregates and photosynthetic proteins.


Assuntos
Fotossíntese , Vibração , Lasers
5.
Mikrochim Acta ; 189(7): 259, 2022 06 15.
Artigo em Inglês | MEDLINE | ID: mdl-35704127

RESUMO

A rapid and effective technique has been develped for the fabrication of sensor-active copper-based materials on the surface of such flexible polymers as terephthalate, polyethylene naphthalate, and polyimide using the method of laser surface modification. For this purpose, we optimized the polymer surface activation parameters using laser sources with a picosecond pulse duration for subsequent selective metallization within the activated region. Furthermore, the fabricated copper structures were modified with gold nanostructures and by electrochemical passivation to produce copper-gold and oxide-containing copper species, respectively. As a result, in comparison with pure copper electrodes, these composite materials exhibit much better electrocatalytic performance concerning the non-enzymatic identification of biologically important disease markers such as glucose, hydrogen peroxide, and dopamine.


Assuntos
Técnicas Biossensoriais , Técnicas Eletroquímicas , Técnicas Biossensoriais/métodos , Cobre/química , Técnicas Eletroquímicas/métodos , Ouro/química , Lasers
6.
Sci Rep ; 12(1): 10535, 2022 Jun 22.
Artigo em Inglês | MEDLINE | ID: mdl-35732689

RESUMO

The intraocular pressure (IOP)-lowering mechanisms of micropulse laser trabeculoplasty (MLT) remain unclear. The present study was performed to investigate the mechanism of action of MLT, and to determine whether the pigmentation intensity of trabecular meshwork (TM) cells is associated with the treatment effects. Primary human TM cells were exposed to melanin granules to artificially introduce different levels of pigmentation. Micropulse (MP) laser irradiation was performed, and interleukin (IL)-1α/ß, matrix metalloproteinases (MMPs), tissue inhibitor of metalloproteinases (TIMPs), and extracellular matrix (ECM) protein expression were evaluated by RT-qPCR and immunocytochemistry. IL-1α/ß and MMP-1, -3, and -9 mRNA expression were significantly upregulated at 4 and 24 h after MP laser irradiation, respectively, but there were no significant changes in TIMP expression. The extent of these upregulation was greater in cells with strong pigmentation intensity. Protein expressions of fibronectin and collagen I were significantly decreased in cells with strong staining intensity. These results suggested that MP laser irradiation alter the MMP/TIMP ratio and enhance ECM turnover, resulting in increased outflow of aqueous humor. The pigmentation intensity of the TM tissues may affect the treatment efficacy of MLT, because TM cells with strong staining intensity showed a significantly enhanced response to MP laser irradiation.


Assuntos
Malha Trabecular , Trabeculectomia , Células Cultivadas , Proteínas da Matriz Extracelular/metabolismo , Humanos , Pressão Intraocular , Lasers , Metaloproteinases da Matriz/genética , Metaloproteinases da Matriz/metabolismo , Pigmentação , Inibidores Teciduais de Metaloproteinases/genética , Malha Trabecular/metabolismo
7.
Methods Mol Biol ; 2505: 45-58, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35732935

RESUMO

The surface-assisted laser desorption/ionization (SALDI) technique uses inorganic materials to aid desorption and ionization of molecules. SALDI is suitable for analyzing small molecules due to the absence of interfering signals in the low m/z range originating from the organic matrix. Imaging mass spectrometry (IMS) is a versatile imaging approach with high spatial resolution for analyzing various molecular species, but its application depends heavily on the ionization method. We have developed a functionalized titanium dioxide (TiO2) nanowire as a solid substrate for SALDI-MS detection of low-molecular-weight molecules. We apply this novel substrate for imprinting fragile specimens such as petals and further SALDI-IMS analysis. The TiO2 nanowire substrate is prepared from a commercial Ti plate by a hydrothermal process and subsequently chemically modified to improve the quality and selectivity of imprinting as well as the sensitivity of SALDI-IMS analysis. Here, the functionalized TiO2 nanowire substrate is applied to visualize the distribution of vinca alkaloids in the petal of Madagascar periwinkle (Catharanthus roseus).


Assuntos
Catharanthus , Alcaloides de Vinca , Diagnóstico por Imagem/métodos , Lasers , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos
8.
Retina ; 42(7): 1356-1363, 2022 07 01.
Artigo em Inglês | MEDLINE | ID: mdl-35723923

RESUMO

PURPOSE: To study the utility of MultiColor confocal scanning laser ophthalmoscope imaging (MCI) in identifying the morphology of uveitic lesions compared with conventional color fundus photography (CFP) in patients with posterior uveitis. METHODS: In this prospective observational study, subjects with posterior uveitis underwent MCI and CFP. The images obtained by the two modalities were analyzed by two independent reviewers for vitreoretinal surface abnormalities, retinal fluid and hemorrhages, and depth/location of lesions. These findings were compared with the clinical findings and other imaging techniques. RESULTS: Sixty-nine eyes of 43 patients (25 men) with mean age of 33.5 ± 13.9 years were studied. MultiColor imaging had better sensitivity and specificity in detecting vitreoretinal interface abnormalities, such as epiretinal membrane and inner retinal striae, compared with CFP. MultiColor imaging failed to detect retinochoroiditis lesions in 5 of 6 eyes (83%) and choroiditis in 9 46 eyes (20%), which were detected on CFP and clinical examination. Also, MCI showed a high false-positive rate of 34% in detecting intraretinal hemorrhages. CONCLUSION: Retinochoroidal lesions in posterior uveitis may be poorly identified on MCI compared with CFP and clinical examination. One must exercise caution in commenting on disease morphology based on MCI alone.


Assuntos
Tomografia de Coerência Óptica , Uveíte Posterior , Adulto , Angiofluoresceinografia/métodos , Fundo de Olho , Humanos , Lasers , Masculino , Pessoa de Meia-Idade , Oftalmoscópios , Oftalmoscopia/métodos , Estudos Retrospectivos , Tomografia de Coerência Óptica/métodos , Uveíte Posterior/diagnóstico , Adulto Jovem
9.
Twin Res Hum Genet ; 25(1): 45-49, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-35644997

RESUMO

The aim of this study was to describe the neonatal management and outcome in monochorionic twins with twin-to-twin transfusion syndrome (TTTS) not treated with fetoscopic laser surgery. All consecutive live-born neonates with TTTS managed at our center between 2002 and 2021 were included in this retrospective study. Neonatal outcome was assessed in 44 twin pairs with TTTS not treated with laser (nonlaser group) compared to a control group of 88 twin pairs with TTTS successfully treated with laser (laser group), matched for gestational age at birth. Primary outcome was adverse neonatal outcome, a composite outcome including neonatal mortality or severe neonatal morbidity. The incidence of adverse neonatal outcome in the nonlaser group and laser group was 30% (26/88) and 11% (19/176), respectively (relative risk = 3.46, 95% CI [1.79, 6.71]). In the nonlaser group, 11% had necrotizing enterocolitis (vs. 2% in the laser group) and 24% had hypotension (vs. 10% in the laser group). Recipients in the nonlaser group had, compared to recipients in the laser group, significantly more severe cerebral injury (18% vs. 5%) and more polycythemia at birth (21% vs. 1%). Donors in the nonlaser group had, compared to donors in the laser group, more severe growth restriction (71% vs 42%), renal failure (11% vs 1%), and anemia at birth (25% vs. 7%). Thus, the risk for neonatal mortality and/or severe morbidity is three-fold higher in TTTS not treated with laser than in TTTS treated with laser, which highlights the fact that these neonates with TTTS are very sick at birth, requiring accurate and prompt intensive treatment.


Assuntos
Transfusão Feto-Fetal , Terapia a Laser , Feminino , Transfusão Feto-Fetal/cirurgia , Fetoscopia , Humanos , Recém-Nascido , Lasers , Gravidez , Estudos Retrospectivos
10.
Acta Crystallogr F Struct Biol Commun ; 78(Pt 6): 226-231, 2022 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-35647679

RESUMO

The enzymatic degradation of semi-cellulosic substrates has recently received immense attention. The enzyme endo-1,4-ß-xylanase is essential for the complete digestion of complex and heterogeneous hemicellulose. Here, the purification, crystallization and preliminary X-ray free-electron laser (XFEL) diffraction analysis of endo-1,4-ß-xylanase from the fungus Hypocrea virens (HviGH11) are reported. Codon-optimized HviGH11 was overexpressed in Escherichia coli and spontaneously crystallized after His-tag purification and concentration. Preliminary XFEL diffraction data were collected at the Pohang Accelerator Laboratory XFEL (PAL-XFEL). A total of 1021 images containing Bragg peaks were obtained and indexed. The HviGH11 crystals belonged to the orthorhombic space group P212121, with unit-cell parameters a = 43.80, b = 51.90, c = 94.90 Å. Using 956 diffraction patterns, the phasing problem was solved and an initial model structure of HviGH11 was obtained.


Assuntos
Endo-1,4-beta-Xilanases , Hypocrea , Cristalização/métodos , Cristalografia por Raios X , Endo-1,4-beta-Xilanases/química , Escherichia coli/genética , Escherichia coli/metabolismo , Hypocrea/genética , Lasers
11.
BMC Ophthalmol ; 22(1): 252, 2022 Jun 07.
Artigo em Inglês | MEDLINE | ID: mdl-35668416

RESUMO

BACKGROUND: To identify the OCT biomarkers related to the anatomical outcomes in eyes with central serous chorioretinopathy (CSCR) after subthreshold micropulse laser (SML) treatment. METHODS: Patients with CSCR underwent SML were enrolled in this retrospective study. Only patients who underwent enhanced depth imaging optical coherence tomography (EDI-OCT) examination before and after SML were selected. Patients were divided into two groups based on whether subretinal fluid (SRF) absorbed or not after SML. Group 1 was the SRF resolved group, and Group 2 was the SRF non-resolved group. Factors including age and gender, duration of symptoms, CSCR history, the height of SRF at baseline, retinal pigment epithelium (RPE) /inner choroid alterations, as well as subfoveal choroidal thickness (SFCT) of the affected eye and the fellow eye before and after SML were recorded and compared between two groups. Longitudinal change of SFCT of a subgroup of patients were analyzed. RESULTS: A total of 58 eyes of 58 patients were involved in this study. SRF of 31 eyes got completely absorbed, and SRF of 27 eyes was retained after SML. Logistic regression analysis revealed baseline SFCT of the affected eye (OR = 1.007, 95% CI: 1.001-1.012, P = 0.019) and RPE/inner choroid alterations (OR = 25.229, 95% CI: 2.890-220.281, P = 0.004) were correlated with SML efficacy. Thirty-three eyes of 33 patients were enrolled in the subgroup analysis. A significant difference of SFCT changes between two groups were demonstrated (P = 0.001). The difference of SFCT between baseline and three months after SML was also related to SRF resolution (OR = 0.952, 95% CI: 0.915-0.990, P = 0.014). CONCLUSION: Baseline SFCT, change of SFCT at 3-month after treatment, and RPE/inner choroid alterations were the OCT biomarkers related to SRF resolution after SML treatment.


Assuntos
Coriorretinopatia Serosa Central , Biomarcadores , Coriorretinopatia Serosa Central/diagnóstico , Coriorretinopatia Serosa Central/cirurgia , Corioide , Angiofluoresceinografia , Humanos , Lasers , Estudos Retrospectivos , Tomografia de Coerência Óptica
12.
Sensors (Basel) ; 22(11)2022 May 27.
Artigo em Inglês | MEDLINE | ID: mdl-35684676

RESUMO

CycleGAN is widely used in various image translations, such as thermal-infrared-visible-image translation, near-infrared-visible-image translation, and shortwave-infrared-visible-image translation. However, most image translations are used for infrared-to-visible translation, and the wide application of laser imaging has an increasingly strong demand for laser-visible image translation. In addition, the current image translation is mainly aimed at frontal face images, which cannot be effectively utilized to translate faces at a certain angle. In this paper, we construct a laser-visible face mapping dataset; in case of the gradient dispersion of the objective function of the original adversarial loss, the least squares loss function is used to replace the cross-entropy loss function and an identity loss function is added to strengthen the network constraints on the generator. The experimental results indicate that the SSIM value of the improved model increases by 1.25%, 8%, 0, 8%, the PSNR value is not much different, and the FID value decreases by 11.22, 12.85, 43.37 and 72.19, respectively, compared with the CycleGAN, Pix2pix, U-GAN-IT and StarGAN models. In the profile image translation, in view of the poor extraction effect of CycleGAN's original feature extraction module ResNet, the RRDB module is used to replace it based on the first improvement. The experimental results show that, compared with the CycleGAN, Pix2pix, U-GAN-IT, StarGAN and the first improved model, the SSIM value of the improved model increased by 3.75%, 10.67%, 2.47%, 10.67% and 2.47%, respectively; the PSNR value increased by 1.02, 2.74, 0.32, 0.66 and 0.02, respectively; the FID value reduced by 26.32, 27.95, 58.47, 87.29 and 15.1, respectively. Subjectively, the contour features and facial features were better conserved.


Assuntos
Processamento de Imagem Assistida por Computador , Lasers , Processamento de Imagem Assistida por Computador/métodos
13.
Sensors (Basel) ; 22(11)2022 May 29.
Artigo em Inglês | MEDLINE | ID: mdl-35684746

RESUMO

The main goal of this work was to modify the previously developed blade-type planar structure using plasmonic gold nanostars in order to stimulate photofield emission and provide efficient laser control of the electron current. Localization and enhancement of the field at the tips of gold nanostars provided a significant increase in the tunneling electron current in the experimental sample (both electrical field and photofield emission). Irradiation at a wavelength in the vicinity of the plasmon resonance (red laser) provided a gain in the photoresponse value of up to 5 times compared to irradiation far from the resonance (green laser). The prospects for transition to regimes of structure irradiation by femtosecond laser pulses at the wavelength of surface plasmon resonance, which lead to an increase in the local optical field, are discussed. The kinetics of the energy density of photoinduced hot and thermalized electrons is estimated. The proposed laser-controlled matrix current source is promising for use in X-ray computed tomography systems.


Assuntos
Ouro , Nanopartículas Metálicas , Elétrons , Ouro/química , Lasers , Nanopartículas Metálicas/química , Ressonância de Plasmônio de Superfície/métodos
14.
Sensors (Basel) ; 22(11)2022 May 29.
Artigo em Inglês | MEDLINE | ID: mdl-35684751

RESUMO

Laser welding is an indispensable link in most types of industrial production. The realization of welding automation by industrial robots can greatly improve production efficiency. In the research and development of the welding seam tracking system, information on the position of the weld joint needs to be obtained accurately. For laser welding images with strong and complex interference, a weld tracking module was designed to capture real-time images of the weld, and a total of 737, 1920 × 1200 pixel weld images were captured using the device, of which 637 were used to create the dataset, and the other 100 were used as images to test the segmentation success rate. Based on the pixel-level segmentation capability of the semantic segmentation network, this study used an encoder-decoder architecture to design a lightweight network structure and introduced a channel attention mechanism. Compared to ERF-Net, SegNet, and DFA-Net, the network model in this paper has a fast segmentation speed and higher segmentation accuracy, with a success rate of 96% and remarkable segmentation results.


Assuntos
Processamento de Imagem Assistida por Computador , Soldagem , Automação , Processamento de Imagem Assistida por Computador/métodos , Lasers , Semântica
15.
Sensors (Basel) ; 22(11)2022 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-35684767

RESUMO

With the remarkable advances in vertical-cavity surface-emitting lasers (VCSELs) in recent decades, VCSELs have been considered promising light sources in the field of optical wireless communications. However, off-the-shelf VCSELs still have a limited modulation bandwidth to meet the multi-Gb/s data rate requirements imposed on the next-generation wireless communication system. Recently, employing machine learning (ML) techniques as a method to tackle such issues has been intriguing for researchers in wireless communication. In this work, through a systematic analysis, it is shown that the ML technique is also very effective in VCSEL-based visible light communication. Using a commercial VCSEL and bidirectional long short-term memory (Bi-LSTM)-based ML scheme, a high-speed visible light communication (VLC) link with a data rate of 13.5 Gbps is demonstrated, which is the fastest single channel result from a cost-effective, off-the-shelf VCSEL device, to the best of the authors' knowledge.


Assuntos
Lasers , Luz , Comunicação , Desenho de Equipamento , Redes Neurais de Computação
16.
J Refract Surg ; 38(6): 332-338, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35686713

RESUMO

PURPOSE: To compare visual outcomes and optical quality of femtosecond laser-assisted small incision lenticule extraction (SMILE) and Visian Implantable Collamer Lens (ICL V4c; STAAR Surgical) implantation for moderate to high myopia. METHODS: For this systematic review and meta-analysis, the Cochrane, PubMed, Embase, and Chinese databases (Wan-fang data and CNKI) were comprehensively searched to identify studies comparing SMILE and ICL V4c implantation. Data of interest were extracted and analyzed by Stata V.16.0 software (StataCorp). The weighted mean differences and their 95% confidence intervals were used to assess the strength of the association. RESULTS: Twelve studies incorporating 1,390 eyes of 822 patients were included. The analysis revealed ICL V4c implantation had a significantly higher safety index and lower high order aberrations, coma, and spherical aberration than SMILE in treating moderate to high myopia (P < .05). Subgroup analysis revealed such differences were more prominent in patients with high myopia who had shorter follow-up duration. When follow-up was longer than 6 months, the differences in safety index and total HOAs became nonsignificant (P > .05). The two procedures had no difference in efficacy index, postoperative visual acuity, spherical equivalent, trefoil, Objective Scatter Index, or modulation transfer function cut-off frequency (P > .05). CONCLUSIONS: Both SMILE and ICL V4c implantation were safe and efficient for moderate to high myopia. Compared with SMILE, ICL V4c implantation might provide better postoperative visual quality in patients with high myopia in the early postoperative period. [J Refract Surg. 2022;38(6):332-338.].


Assuntos
Lentes Intraoculares , Miopia , Humanos , Lasers , Implante de Lente Intraocular/métodos , Miopia/cirurgia , Refração Ocular
17.
Nature ; 606(7912): 94-101, 2022 06.
Artigo em Inglês | MEDLINE | ID: mdl-35650358

RESUMO

Neurotransmitters play essential roles in regulating neural circuit dynamics both in the central nervous system as well as at the peripheral, including the gastrointestinal tract1-3. Their real-time monitoring will offer critical information for understanding neural function and diagnosing disease1-3. However, bioelectronic tools to monitor the dynamics of neurotransmitters in vivo, especially in the enteric nervous systems, are underdeveloped. This is mainly owing to the limited availability of biosensing tools that are capable of examining soft, complex and actively moving organs. Here we introduce a tissue-mimicking, stretchable, neurochemical biological interface termed NeuroString, which is prepared by laser patterning of a metal-complexed polyimide into an interconnected graphene/nanoparticle network embedded in an elastomer. NeuroString sensors allow chronic in vivo real-time, multichannel and multiplexed monoamine sensing in the brain of behaving mouse, as well as measuring serotonin dynamics in the gut without undesired stimulations and perturbing peristaltic movements. The described elastic and conformable biosensing interface has broad potential for studying the impact of neurotransmitters on gut microbes, brain-gut communication and may ultimately be extended to biomolecular sensing in other soft organs across the body.


Assuntos
Encéfalo , Sistema Nervoso Entérico , Trato Gastrointestinal , Neurotransmissores , Animais , Técnicas Biossensoriais , Encéfalo/metabolismo , Elastômeros , Sistema Nervoso Entérico/metabolismo , Trato Gastrointestinal/inervação , Trato Gastrointestinal/fisiologia , Grafite , Lasers , Camundongos , Nanopartículas , Neurotransmissores/análise , Serotonina/análise
18.
Sci Rep ; 12(1): 9506, 2022 Jun 09.
Artigo em Inglês | MEDLINE | ID: mdl-35681067

RESUMO

We propose two novel methods to effectively utilize parylene C films. First, we demonstrate a vertical deposition method capable of depositing a parylene C film of the same thickness on both sides of a sample. Through this method, we have formed parylene C films with a thickness of 4 µm on both sides of the sample with a thickness deviation of less than 2.5%. Further optical verification indicates that parylene C films formed by this method have a very uniform thickness distribution on each side of the surfaces. Second, we propose a debris-tolerant laser patterning method as a mask-less means to fabricate self-supporting ultrathin parylene C films. This method does not involve any photolithography and entails a simple and rapid process that can be performed using only a few materials with excellent biocompatibility. It is demonstrated that patterned parylene C films exhibit a high degree of surface uniformity and have various geometrical shapes so that they can be used for substrates of highly flexible and/or stretchable devices. Finally, we use both of the proposed methods to fabricate flexible, stretchable, and waterproof-packaged bifacial blue LED modules to illustrate their potential in emerging applications that would benefit from such versatile form factors.


Assuntos
Polímeros , Xilenos , Lasers
19.
BMC Ophthalmol ; 22(1): 258, 2022 Jun 09.
Artigo em Inglês | MEDLINE | ID: mdl-35681133

RESUMO

BACKGROUND: The treatment for diabetic macular edema (DME) has revolutionized during the last 15 years after the introduction of intravitreal anti-VEGF agents. The aim of the current study is to evaluate the real-world visual outcomes of diabetic macular edema (DME) treatment in patients with type 1 diabetes (T1D) in long-term follow-up. METHODS: A real-world, descriptive, population-based cohort and follow-up of all patients with T1D and DME in 2006-2020 in 34 communities of the Northern Ostrobothnia Hospital District. The main outcome measures included age, gender, duration of T1D at the onset of DME, stage of retinopathy, treatment of DME (observation, laser, intravitreal treatments, combination), and visual outcomes. RESULTS: A total of 304 eyes of 206 T1D patients with DME were included. 75% (n=155) had non-proliferative diabetic retinopathy during the onset of DME. 15% of the cases were observed, 33% had macular laser, 41% intravitreal anti-VEGF and 12% combination of laser and intravitreal injections. Patients in anti-VEGF and in combination groups gained 4.9 and 5.5 ETDRS letters after the initial DME episode (p<0.001 and p<0.001), and the long-term visual improvements were 4.1 and 5.1 ETDRS letters (p<0.001 and p<0.001), respectively. In observation and laser groups the initial gain of 0.1 (p>0.90) and loss of 0.4 ETDRS letter (p=0.61), respectively, was noted. After the follow-up, a 3.7 ETDRS letter decrease was documented in the observation group (p>0.90) and a 1.1 (p=0.14) ETDRS letter decline in the laser group of patients. At the beginning of treatment, eyes subjected to anti-VEGF alone or in combination with laser had lower visual acuity compared to eyes subjected to observation or macular laser. The average of a 6.1±4.8 anti-VEGF injections were needed to dry DME. Visual impairment due to DME decreased from 2.4% to 1.0% during the 15-year period. CONCLUSIONS: Anti-VEGF alone or in combination with macular laser seems to be beneficial in terms of visual outcomes and treatment stability in T1D patients with central DME. Moreover, satisfying long-term visual outcomes were achieved with anti-VEGF treatment in a real-world setting.


Assuntos
Diabetes Mellitus Tipo 1 , Retinopatia Diabética , Edema Macular , Inibidores da Angiogênese , Anticorpos Monoclonais/uso terapêutico , Diabetes Mellitus Tipo 1/complicações , Retinopatia Diabética/complicações , Retinopatia Diabética/tratamento farmacológico , Humanos , Injeções Intravítreas , Lasers , Edema Macular/tratamento farmacológico , Edema Macular/etiologia , Ranibizumab/uso terapêutico , Resultado do Tratamento
20.
Int J Mol Sci ; 23(11)2022 May 25.
Artigo em Inglês | MEDLINE | ID: mdl-35682611

RESUMO

Cisplatin is one of the most widely used anticancer drugs in the treatment of various types of solid human cancers, as well as germ cell tumors, sarcomas, and lymphomas. Strong evidence from research has demonstrated higher efficacy of a combination of cisplatin and derivatives, together with hyperthermia and light, in overcoming drug resistance and improving tumoricidal efficacy. It is well known that the antioncogenic potential of CDDP is markedly enhanced by hyperthermia compared to drug treatment alone. However, more recently, accelerators of high energy particles, such as synchrotrons, have been used to produce powerful and monochromatizable radiation to induce an Auger electron cascade in cis-platinum molecules. This is the concept that makes photoactivation of cis-platinum theoretically possible. Both heat and light increase cisplatin anticancer activity via multiple mechanisms, generating DNA lesions by interacting with purine bases in DNA followed by activation of several signal transduction pathways which finally lead to apoptosis. For the past twenty-seven years, our group has developed infrared photo-thermal activation of cisplatin for cancer treatment from bench to bedside. The future development of photoactivatable prodrugs of platinum-based agents injected intratumorally will increase selectivity, lower toxicity and increase efficacy of this important class of antitumor drugs, particularly when treating tumors accessible to laser-based fiber-optic devices, as in head and neck cancer. In this article, the mechanistic rationale of combined intratumor injections of cisplatin and laser-induced thermal therapy (CDDP-LITT) and the clinical application of such minimally invasive treatment for cancer are reviewed.


Assuntos
Antineoplásicos , Neoplasias de Cabeça e Pescoço , Antineoplásicos/farmacologia , Antineoplásicos/uso terapêutico , Cisplatino/farmacologia , DNA , Neoplasias de Cabeça e Pescoço/tratamento farmacológico , Humanos , Lasers
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