Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 15.516
Filtrar
Mais filtros








Intervalo de ano de publicação
1.
PLoS One ; 19(5): e0302514, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38718004

RESUMO

Expanding spatial presentation from two-dimensional profile transects to three-dimensional ocean mapping is key for a better understanding of ocean processes. Phytoplankton distributions can be highly patchy and the accurate identification of these patches with the context, variability, and uncertainty of measurements on relevant scales is difficult to achieve. Traditional sampling methods, such as plankton nets, water samplers and in-situ vertical sensors, provide a snapshot and often miss the fine-scale horizontal and temporal variability. Here, we show how two autonomous underwater vehicles measured, adapted to, and reported real-time chlorophyll a measurements, giving insights into the spatiotemporal distribution of phytoplankton biomass and patchiness. To gain the maximum available information within their sensing scope, the vehicles moved in an adaptive fashion, looking for the regions of the highest predicted chlorophyll a concentration, the greatest uncertainty, and the least possibility of collision with other underwater vehicles and ships. The vehicles collaborated by exchanging data with each other and operators via satellite, using a common segmentation of the area to maximize information exchange over the limited bandwidth of the satellite. Importantly, the use of multiple autonomous underwater vehicles reporting real-time data combined with targeted sampling can provide better match with sampling towards understanding of plankton patchiness and ocean processes.


Assuntos
Clorofila A , Oceanos e Mares , Fitoplâncton , Clorofila A/análise , Monitoramento Ambiental/métodos , Clorofila/análise , Biomassa , Imageamento Tridimensional/métodos
2.
PeerJ ; 12: e17361, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38737741

RESUMO

Phytoplankton are the world's largest oxygen producers found in oceans, seas and large water bodies, which play crucial roles in the marine food chain. Unbalanced biogeochemical features like salinity, pH, minerals, etc., can retard their growth. With advancements in better hardware, the usage of Artificial Intelligence techniques is rapidly increasing for creating an intelligent decision-making system. Therefore, we attempt to overcome this gap by using supervised regressions on reanalysis data targeting global phytoplankton levels in global waters. The presented experiment proposes the applications of different supervised machine learning regression techniques such as random forest, extra trees, bagging and histogram-based gradient boosting regressor on reanalysis data obtained from the Copernicus Global Ocean Biogeochemistry Hindcast dataset. Results obtained from the experiment have predicted the phytoplankton levels with a coefficient of determination score (R2) of up to 0.96. After further validation with larger datasets, the model can be deployed in a production environment in an attempt to complement in-situ measurement efforts.


Assuntos
Aprendizado de Máquina , Fitoplâncton , Tecnologia de Sensoriamento Remoto , Tecnologia de Sensoriamento Remoto/métodos , Tecnologia de Sensoriamento Remoto/instrumentação , Oceanos e Mares , Monitoramento Ambiental/métodos , Aprendizado de Máquina Supervisionado
3.
Environ Sci Technol ; 58(19): 8510-8517, 2024 May 14.
Artigo em Inglês | MEDLINE | ID: mdl-38695484

RESUMO

Anthropogenic activities have fundamentally changed the chemistry of the Baltic Sea. According to results reported in this study, not even the thallium (Tl) isotope cycle is immune to these activities. In the anoxic and sulfidic ("euxinic") East Gotland Basin today, Tl and its two stable isotopes are cycled between waters and sediments as predicted based on studies of other redox-stratified basins (e.g., the Black Sea and Cariaco Trench). The Baltic seawater Tl isotope composition (ε205Tl) is, however, higher than predicted based on the results of conservative mixing calculations. Data from a short sediment core from East Gotland Basin demonstrates that this high seawater ε205Tl value originated sometime between about 1940 and 1947 CE, around the same time other prominent anthropogenic signatures begin to appear in the same core. This juxtaposition is unlikely to be coincidental and suggests that human activities in the surrounding area have altered the seawater Tl isotope mass-balance of the Baltic Sea.


Assuntos
Sedimentos Geológicos , Oceanos e Mares , Água do Mar , Tálio , Água do Mar/química , Sedimentos Geológicos/química , Atividades Humanas , Humanos , Monitoramento Ambiental , Poluentes Químicos da Água , Isótopos
4.
Proc Biol Sci ; 291(2023): 20232207, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38772423

RESUMO

Population and species persistence in a rapidly warming world will be determined by an organism's ability to acclimate to warmer conditions, especially across generations. There is potential for transgenerational acclimation but the importance of ontogenetic timing in the transmission of environmentally induced parental effects remains mostly unknown. We aimed to disentangle the effects of two critical ontogenetic stages (juvenile development and reproduction) to the new-generation acclimation potential, by exposing the spiny chromis damselfish Acanthochromis polyacanthus to simulated ocean warming across two generations. By using hepatic transcriptomics, we discovered that the post-hatching developmental environment of the offspring themselves had little effect on their acclimation potential at 2.5 months of life. Instead, the developmental experience of parents increased regulatory RNA production and protein synthesis, which could improve the offspring's response to warming. Conversely, parental reproduction and offspring embryogenesis in warmer water elicited stress response mechanisms in the offspring, with suppression of translation and mitochondrial respiration. Mismatches between parental developmental and reproductive temperatures deeply affected offspring gene expression profiles, and detrimental effects were evident when warming occurred both during parents' development and reproduction. This study reveals that the previous generation's developmental temperature contributes substantially to thermal acclimation potential during early life; however, exposure at reproduction as well as prolonged heat stress will likely have adverse effects on the species' persistence.


Assuntos
Aclimatação , Recifes de Corais , Animais , Reprodução , Aquecimento Global , Perciformes/fisiologia , Transcriptoma , Oceanos e Mares , Peixes/fisiologia , Temperatura
5.
Proc Natl Acad Sci U S A ; 121(19): e2319937121, 2024 May 07.
Artigo em Inglês | MEDLINE | ID: mdl-38696469

RESUMO

Subtropical oceans contribute significantly to global primary production, but the fate of the picophytoplankton that dominate in these low-nutrient regions is poorly understood. Working in the subtropical Mediterranean, we demonstrate that subduction of water at ocean fronts generates 3D intrusions with uncharacteristically high carbon, chlorophyll, and oxygen that extend below the sunlit photic zone into the dark ocean. These contain fresh picophytoplankton assemblages that resemble the photic-zone regions where the water originated. Intrusions propagate depth-dependent seasonal variations in microbial assemblages into the ocean interior. Strikingly, the intrusions included dominant biomass contributions from nonphotosynthetic bacteria and enrichment of enigmatic heterotrophic bacterial lineages. Thus, the intrusions not only deliver material that differs in composition and nutritional character from sinking detrital particles, but also drive shifts in bacterial community composition, organic matter processing, and interactions between surface and deep communities. Modeling efforts paired with global observations demonstrate that subduction can flux similar magnitudes of particulate organic carbon as sinking export, but is not accounted for in current export estimates and carbon cycle models. Intrusions formed by subduction are a particularly important mechanism for enhancing connectivity between surface and upper mesopelagic ecosystems in stratified subtropical ocean environments that are expanding due to the warming climate.


Assuntos
Bactérias , Oceanos e Mares , Água do Mar , Água do Mar/microbiologia , Água do Mar/química , Bactérias/metabolismo , Carbono/metabolismo , Ciclo do Carbono , Clorofila/metabolismo , Ecossistema , Fitoplâncton/metabolismo , Estações do Ano , Biomassa , Microbiota/fisiologia , Oxigênio/metabolismo
6.
Nat Commun ; 15(1): 3979, 2024 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-38729972

RESUMO

A primary response of many marine ectotherms to warming is a reduction in body size, to lower the metabolic costs associated with higher temperatures. The impact of such changes on ecosystem dynamics and stability will depend on the resulting changes to community size-structure, but few studies have investigated how temperature affects the relative size of predators and their prey in natural systems. We utilise >3700 prey size measurements from ten Southern Ocean lanternfish species sampled across >10° of latitude to investigate how temperature influences predator-prey size relationships and size-selective feeding. As temperature increased, we show that predators became closer in size to their prey, which was primarily associated with a decline in predator size and an increase in the relative abundance of intermediate-sized prey. The potential implications of these changes include reduced top-down control of prey populations and a reduction in the diversity of predator-prey interactions. Both of these factors could reduce the stability of community dynamics and ecosystem resistance to perturbations under ocean warming.


Assuntos
Tamanho Corporal , Peixes , Oceanos e Mares , Comportamento Predatório , Temperatura , Animais , Comportamento Predatório/fisiologia , Tamanho Corporal/fisiologia , Peixes/fisiologia , Cadeia Alimentar , Ecossistema , Dinâmica Populacional
7.
PLoS One ; 19(5): e0302305, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38722994

RESUMO

This article proposes an integer ambiguity determination method based on Beidou system-reflectometry (Beidou-R) observations of the carrier phase at the B1I and B3I frequencies. To enhance the accuracy of sea surface height (SSH) estimation, this study introduces a parallel filtering algorithm and an adaptive iterative fusion algorithm, enabling data fusion based on the variance at B1I and B3I frequencies. To validate and evaluate the proposed method, a coastal experiment was conducted at the Shenxian River. In this experiment, reflected signals from GEO and IGSO satellites were collected. Data analysis reveals that the method is effective, demonstrating that the root mean square error (RMSE) of SSH achieves 2.85 cm and 2.89 cm for PRN 04 and PRN 33, respectively. Furthermore, the impact of the elevation angle on measurement accuracy is analyzed. This study aims to propose a method to enhance coastal sea surface height estimation, offering potential advancements in sea surface altimetry.


Assuntos
Algoritmos , Oceanos e Mares , Monitoramento Ambiental/métodos
9.
Commun Biol ; 7(1): 611, 2024 May 21.
Artigo em Inglês | MEDLINE | ID: mdl-38773323

RESUMO

Human impacts lead to widespread changes in the abundance, diversity and traits of shark assemblages, altering the functioning of coastal ecosystems. The functional consequences of shark declines are often poorly understood due to the absence of empirical data describing long-term change. We use data from the Queensland Shark Control Program in eastern Australia, which has deployed mesh nets and baited hooks across 80 beaches using standardised methodologies since 1962. We illustrate consistent declines in shark functional richness quantified using both ecological (e.g., feeding, habitat and movement) and morphological (e.g., size, morphology) traits, and this corresponds with declining ecological functioning. We demonstrate a community shift from targeted apex sharks to a greater functional richness of non-target species. Declines in apex shark functional richness and corresponding changes in non-target species may lead to an anthropogenically induced trophic cascade. We suggest that repairing diminished shark populations is crucial for the stability of coastal ecosystems.


Assuntos
Biodiversidade , Tubarões , Tubarões/fisiologia , Animais , Queensland , Ecossistema , Dinâmica Populacional , Austrália , Oceanos e Mares
11.
Glob Chang Biol ; 30(5): e17337, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38771026

RESUMO

Persistently high marine temperatures are escalating and threating marine biodiversity. The Baltic Sea, warming faster than other seas, is a good model to study the impact of increasing sea surface temperatures. Zostera marina, a key player in the Baltic ecosystem, faces susceptibility to disturbances, especially under chronic high temperatures. Despite the increasing number of studies on the impact of global warming on seagrasses, little attention has been paid to the role of the holobiont. Using an outdoor benthocosm to replicate near-natural conditions, this study explores the repercussions of persistent warming on the microbiome of Z. marina and its implications for holobiont function. Results show that both seasonal warming and chronic warming, impact Z. marina roots and sediment microbiome. Compared with roots, sediments demonstrate higher diversity and stability throughout the study, but temperature effects manifest earlier in both compartments, possibly linked to premature Z. marina die-offs under chronic warming. Shifts in microbial composition, such as an increase in organic matter-degrading and sulfur-related bacteria, accompany chronic warming. A higher ratio of sulfate-reducing bacteria compared to sulfide oxidizers was found in the warming treatment which may result in the collapse of the seagrasses, due to toxic levels of sulfide. Differentiating predicted pathways for warmest temperatures were related to sulfur and nitrogen cycles, suggest an increase of the microbial metabolism, and possible seagrass protection strategies through the production of isoprene. These structural and compositional variations in the associated microbiome offer early insights into the ecological status of seagrasses. Certain taxa/genes/pathways may serve as markers for specific stresses. Monitoring programs should integrate this aspect to identify early indicators of seagrass health. Understanding microbiome changes under stress is crucial for the use of potential probiotic taxa to mitigate climate change effects. Broader-scale examination of seagrass-microorganism interactions is needed to leverage knowledge on host-microbe interactions in seagrasses.


Assuntos
Microbiota , Zosteraceae , Zosteraceae/microbiologia , Raízes de Plantas/microbiologia , Sedimentos Geológicos/microbiologia , Temperatura Alta , Aquecimento Global , Oceanos e Mares , Bactérias/classificação , Bactérias/isolamento & purificação , Estações do Ano , Mudança Climática
12.
Nature ; 629(8012): 603-608, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38750234

RESUMO

Natural iron fertilization of the Southern Ocean by windblown dust has been suggested to enhance biological productivity and modulate the climate1-3. Yet, this process has never been quantified across the Southern Ocean and at annual timescales4,5. Here we combined 11 years of nitrate observations from autonomous biogeochemical ocean profiling floats with a Southern Hemisphere dust simulation to empirically derive the relationship between dust-iron deposition and annual net community production (ANCP) in the iron-limited Southern Ocean. Using this relationship, we determined the biological response to dust-iron in the pelagic perennially ice-free Southern Ocean at present and during the last glacial maximum (LGM). We estimate that dust-iron now supports 33% ± 15% of Southern Ocean ANCP. During the LGM, when dust deposition was 5-40-fold higher than today, the contribution of dust to Southern Ocean ANCP was much greater, estimated at 64% ± 13%. We provide quantitative evidence of basin-wide dust-iron fertilization of the Southern Ocean and the potential magnitude of its impact on glacial-interglacial timescales, supporting the idea of the important role of dust in the global carbon cycle and climate6-8.


Assuntos
Poeira , Ferro , Oceanos e Mares , Água do Mar , Poeira/análise , Ferro/análise , Água do Mar/química , Água do Mar/análise , Nitratos/análise , Camada de Gelo/química , Ciclo do Carbono , Animais
13.
Science ; 384(6697): 734-737, 2024 May 17.
Artigo em Inglês | MEDLINE | ID: mdl-38753785

RESUMO

Comprehensive spatial planning in international waters is key to achieving ocean sustainability.


Assuntos
Oceanos e Mares , Mudança Climática , Conservação dos Recursos Naturais
14.
Sci Adv ; 10(20): eadm9511, 2024 May 17.
Artigo em Inglês | MEDLINE | ID: mdl-38748799

RESUMO

Helical motion is prevalent in nature and has been shown to confer stability and efficiency in microorganisms. However, the mechanics of helical locomotion in larger organisms (>1 centimeter) remain unknown. In the open ocean, we observed the chain forming salp, Iasis cylindrica, swimming in helices. Three-dimensional imaging showed that helicity derives from torque production by zooids oriented at an oblique orientation relative to the chain axis. Colonies can spin both clockwise and counterclockwise and longer chains (>10 zooids) transition from spinning around a linear axis to a helical swimming path. Propulsive jets are non-interacting and directed at a small angle relative to the axis of motion, thus maximizing thrust while minimizing destructive interactions. Our integrated approach reveals the biomechanical advantages of distributed propulsion and macroscale helical movement.


Assuntos
Oceanos e Mares , Fenômenos Biomecânicos , Natação/fisiologia
15.
Sci Total Environ ; 932: 173000, 2024 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-38719050

RESUMO

Ocean acidification and warming affect marine ecosystems from the molecular scale in organismal physiology to broad alterations of ecosystem functions. However, knowledge of their combined effects on tropical-subtropical intertidal species remains limited. Pushing the environmental range of marine species away from the optimum initiates stress impacting biochemical metabolic characteristics, with consequences on lipid-associated and enzyme biochemistry. This study investigates lipid-associated fatty acids (FAs) and enzyme activities involved in biomineralization of the tropical-subtropical starfish Aquilonastra yairi in response to projected near-future global change. The starfish were acclimatized to two temperature levels (27 °C, 32 °C) crossed with three pCO2 concentrations (455 µatm, 1052 µatm, 2066 µatm). Total lipid (ΣLC) and FAs composition were unaffected by combined elevated temperature and pCO2, but at elevated temperature, there was an increase in ΣLC, SFAs (saturated FAs) and PUFAs (polyunsaturated FAs), and a decrease in MUFAs (monounsaturated FAs). However, temperature was the sole factor to significantly alter SFAs composition. Positive parabolic responses of Ca-ATPase and Mg-ATPase enzyme activities were detected at 27 °C with elevated pCO2, while stable enzyme activities were observed at 32 °C with elevated pCO2. Our results indicate that the lipid-associated biochemistry of A. yairi is resilient and capable of coping with near-future ocean acidification and warming. However, the calcification-related enzymes Ca-ATPase and Mg-ATPase activity appear to be more sensitive to pCO2/pH changes, leading to vulnerability concerning the skeletal structure.


Assuntos
Água do Mar , Estrelas-do-Mar , Animais , Água do Mar/química , Concentração de Íons de Hidrogênio , Estrelas-do-Mar/fisiologia , Aquecimento Global , Dióxido de Carbono , Oceanos e Mares , Mudança Climática , Ácidos Graxos/metabolismo , Metabolismo dos Lipídeos , Acidificação dos Oceanos
17.
Nat Commun ; 15(1): 3715, 2024 May 02.
Artigo em Inglês | MEDLINE | ID: mdl-38698041

RESUMO

Phages play an essential role in controlling bacterial populations. Those infecting Pelagibacterales (SAR11), the dominant bacteria in surface oceans, have been studied in silico and by cultivation attempts. However, little is known about the quantity of phage-infected cells in the environment. Using fluorescence in situ hybridization techniques, we here show pelagiphage-infected SAR11 cells across multiple global ecosystems and present evidence for tight community control of pelagiphages on the SAR11 hosts in a case study. Up to 19% of SAR11 cells were phage-infected during a phytoplankton bloom, coinciding with a ~90% reduction in SAR11 cell abundance within 5 days. Frequently, a fraction of the infected SAR11 cells were devoid of detectable ribosomes, which appear to be a yet undescribed possible stage during pelagiphage infection. We dubbed such cells zombies and propose, among other possible explanations, a mechanism in which ribosomal RNA is used as a resource for the synthesis of new phage genomes. On a global scale, we detected phage-infected SAR11 and zombie cells in the Atlantic, Pacific, and Southern Oceans. Our findings illuminate the important impact of pelagiphages on SAR11 populations and unveil the presence of ribosome-deprived zombie cells as part of the infection cycle.


Assuntos
Bacteriófagos , Ribossomos , Ribossomos/metabolismo , Bacteriófagos/genética , Bacteriófagos/fisiologia , Fitoplâncton/virologia , Fitoplâncton/genética , Fitoplâncton/metabolismo , Hibridização in Situ Fluorescente , Alphaproteobacteria/genética , Alphaproteobacteria/metabolismo , Ecossistema , Água do Mar/microbiologia , Água do Mar/virologia , Oceanos e Mares
18.
Glob Chang Biol ; 30(5): e17296, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38715312

RESUMO

Prospective risks from climate change impacts in ocean and coastal systems are urging the implementation of nature-based solutions (NBS). These are climate-resilient strategies to maintain biodiversity and the delivery of ecosystem services, contributing to the adaptation of social-ecological systems and the mitigation of climate-related impacts. However, the effectiveness of measures like marine restoration or conservation is not exempt from the impacts of climate change, and the degree to which they can sustain biodiversity and ecosystem services remains unknown. Such uncertainty, together with the slow pace of implementation, causes decision-makers and societies to demand a better understanding of NBS effects. To address this gap, in this study, we use the risk mitigation capacity of marine NBS as a proxy for their effectiveness while providing a toolset for the implementation of the method. The method considers environmental data and relies on expert elicitation, allowing us to go beyond current practice to evaluate the effectiveness of NBS in reducing habitat or species risks under different future socio-political and climate-change scenarios. As a result, we present a ready-to-use tool, and supporting materials, for the implementation of the Climate Risk Assessment method and an illustrative example considering the application of the NBS "nature-inclusive harvesting" in two shellfisheries. The method works as a rapid assessment that guarantees comparability across sites and species due to its low data or resource demand, so it can be widely incorporated to adaptation policies across the marine realm.


Assuntos
Biodiversidade , Mudança Climática , Conservação dos Recursos Naturais , Ecossistema , Medição de Risco/métodos , Conservação dos Recursos Naturais/métodos , Oceanos e Mares
19.
PLoS One ; 19(5): e0301709, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38743649

RESUMO

Rogue waves are sudden and extreme occurrences, with heights that exceed twice the significant wave height of their neighboring waves. The formation of rogue waves has been attributed to several possible mechanisms such as linear superposition of random waves, dispersive focusing, and modulational instability. Recently, nonlinear Fourier transforms (NFTs), which generalize the usual Fourier transform, have been leveraged to analyze oceanic rogue waves. Next to the usual linear Fourier modes, NFTs can additionally uncover nonlinear Fourier modes in time series that are usually hidden. However, so far only individual oceanic rogue waves have been analyzed using NFTs in the literature. Moreover, the completely different types of nonlinear Fourier modes have been observed in these studies. Exploiting twelve years of field measurement data from an ocean buoy, we apply the nonlinear Fourier transform (NFT) for the nonlinear Schrödinger equation (NLSE) (referred to NLSE-NFT) to a large dataset of measured rogue waves. While the NLSE-NFT has been used to analyze rogue waves before, this is the first time that it is systematically applied to a large real-world dataset of deep-water rogue waves. We categorize the measured rogue waves into four types based on the characteristics of the largest nonlinear mode: stable, small breather, large breather and (envelope) soliton. We find that all types can occur at a single site, and investigate which conditions are dominated by a single type at the measurement site. The one and two-dimensional Benjamin-Feir indices (BFIs) are employed to examine the four types of nonlinear spectra. Furthermore, we verify on a part of the data set that for the localized types, the largest nonlinear Fourier mode can be attributed directly to the rogue wave, and investigate the relation between the height of the rogue waves and that of the dominant nonlinear Fourier mode. While the dominant nonlinear Fourier mode in general only contributes a small fraction of the rogue wave, we find that soliton modes can contribute up to half of the rogue wave. Since the NLSE does not account for directional spreading, the classification is repeated for the first quartile with the lowest directional spreading for each type. Similar results are obtained.


Assuntos
Análise de Fourier , Oceanos e Mares , Dinâmica não Linear , Filipinas
20.
Nat Commun ; 15(1): 4089, 2024 May 14.
Artigo em Inglês | MEDLINE | ID: mdl-38744831

RESUMO

Dominant microorganisms of the Sargasso Sea are key drivers of the global carbon cycle. However, associated viruses that shape microbial community structure and function are not well characterised. Here, we combined short and long read sequencing to survey Sargasso Sea phage communities in virus- and cellular fractions at viral maximum (80 m) and mesopelagic (200 m) depths. We identified 2,301 Sargasso Sea phage populations from 186 genera. Over half of the phage populations identified here lacked representation in global ocean viral metagenomes, whilst 177 of the 186 identified genera lacked representation in genomic databases of phage isolates. Viral fraction and cell-associated viral communities were decoupled, indicating viral turnover occurred across periods longer than the sampling period of three days. Inclusion of long-read data was critical for capturing the breadth of viral diversity. Phage isolates that infect the dominant bacterial taxa Prochlorococcus and Pelagibacter, usually regarded as cosmopolitan and abundant, were poorly represented.


Assuntos
Bacteriófagos , Metagenoma , Metagenômica , Oceanos e Mares , Água do Mar , Metagenômica/métodos , Bacteriófagos/genética , Bacteriófagos/isolamento & purificação , Bacteriófagos/classificação , Água do Mar/virologia , Água do Mar/microbiologia , Metagenoma/genética , Genoma Viral/genética , Filogenia , Prochlorococcus/virologia , Prochlorococcus/genética , Microbiota/genética , Bactérias/genética , Bactérias/virologia , Bactérias/classificação , Bactérias/isolamento & purificação
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA