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1.
Braz. j. biol ; 84: e258128, 2024. tab, mapas, ilus
Artigo em Inglês | VETINDEX | ID: biblio-1374657

RESUMO

In the western mountainous region of Khyber Pakhtunkhwa Pakistan at the Shangla district, we found Physalis ixocarpa for the first time, not yet reported from Pakistan. Physalis ixocarpa was unidentified and has no ethnobotanical record in the flora of Pakistan. It is a member of family Solanaceae and having a close relation with Solanum tuberosum and Lycopersicon esculentum. The stem is prostrate with a dichotomous pattern of branches having leaves flower and fruits. Leaves are smooth, ovate and the margins of leaf blade dentation are poorly developed. The average length and width of the leaves are 6.50 and 3.61 cm respectively. P. ixocarpa grows to the length of 4-5 feet and an annual herb. The flowers of the plants are yellow in color and having purple color spots on the petals which are star-shaped. The round berry fruits are surrounded by persistent calyx and purple in color. The fruits are the 3-6cm in diameter. The plants are found in the different localities of district Shangla especially in Bar and Koz Kana. The life cycle of reporting plant is started in May and completed in November.


Na região montanhosa ocidental de Khyber Pakhtunkhwa, Paquistão, no distrito de Shangla, encontramos Physalis ixocarpa pela primeira vez, ainda não relatada nesse país. A P. ixocarpa não foi identificada e não possui registro etnobotânico na flora do Paquistão. É membro da família Solanaceae e tem estreita relação com Solanum tuberosum e Lycopersicon esculentum. O caule é rastejante com um padrão dicotômico de ramos com folhas, flores e frutos. As folhas são lisas e ovais e as margens da dentição da lâmina foliar são pouco desenvolvidas. O comprimento e a largura médios das folhas são de 6,50 e 3,61 cm, respectivamente. A P. ixocarpa cresce aproximadamente 120-150 cm de comprimento e é uma planta anual. As flores das plantas são de cor amarela com manchas roxas nas pétalas em forma de estrela. Os frutos redondos da baga são rodeados por cálice persistente e de cor roxa. Os frutos têm 3-6 cm de diâmetro. As plantas são encontradas em diferentes localidades do distrito de Shangla, especialmente em Bar e Koz Kana. O ciclo de vida da planta reportado é iniciado em maio e concluído em novembro.


Assuntos
Registros , Flora , Etnobotânica , Physalis , Paquistão
2.
J Ethnopharmacol ; 300: 115750, 2023 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-36162547

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Different Physalis plants have been widely employed in traditional medicine for management of diabetes mellitus. Previous studies with respect to the in vivo antidiabetic activity of Physalis plants illustrated that they improved glucose and lipid metabolism in streptozotocin (STZ) -induced diabetic rats yet the mechanism of action of bioactive constituents of the different organs of Physalis plants on diabetes remains obscure. AIM OF STUDY: Our objective is to study the effects of the different organs of ground cherry (P. pruinosa) on diabetes in rat models and elucidate their mechanism of actions through serum pharmacochemistry combined to network pharmacology analyses and in-vivo testing. MATERIALS AND METHODS: Characterization of the constituents in the drug-dosed serum samples relative to the blank serum after treatment with different extracts was performed by UPLC -MS/MS technique. The absorbed metabolites where then subjected to network pharmacology analysis to construct an interaction network linking "compound-target-pathway". In vivo verification was implemented to determine a hypothesized mechanism of action on a STZ and high fat diet induced type II diabetes mellitus (T2DM) model based on functional and enrichment analyses of the Kyoto Encyclopedia of Genes and Genome and Gene Ontology. RESULTS: Identification of a total of 73 compounds (22 prototypes and 51 metabolites) derived from P. pruinosa extracts was achieved through comparison of the serum samples collected from diabetic control group and extracts treated groups. The identified compounds were found to belong to different classes according to their structural type including withanolides, physalins and flavonoids. The absorbed compounds in the analyzed serum samples were considered as the potential bioactive components. The component-target network was found to have 23 nodes with 17 target genes including MAPK8, CYP1A1 and CYP1B1. Quercetin and withaferin A were found to possess the highest combined score in the C-T network. Integrated serum pharmacochemistry and network pharmacology analyses revealed the enrichment of leaves extract with the active constituents, which can be utilized in T2DM treatment. In the top KEGG pathways, lipid and atherosclerosis metabolic pathways in addition to T2DM pathways were found to be highly prioritized. The diabetic rats, which received leaves extract exhibited a substantial increment in GLUT2, INSR, IRS-1, PI3K-p85 and AKT-ser473 proteins by 105%, 142%, 109%, 81% and 73%, respectively relative to the untreated diabetic group. The immunoblotting performed for MAPK and ERK1/2 part of the inflammatory pathway studied in STZ induced diabetic rats revealed that leaves, calyces and stems extracts resulted in a substantial diminish in p38-MAPK, ERK 1/2, NF-κB, and TNF-α. Histopathological examination revealed that the hepatic histoarchitecture was substantially improved in the leaves, stems, and clayces-treated rats in comparison with untreated diabetic rats. Further, pancreatic injuries, which induced by STZ were dramatically altered by the treatment with P. pruinosa leaves, calyces and stems extracts. ß-cells in diabetic rats received leaves extract disclosed moderate insulin immunostaining with a notable increase in the mean insulin area%. CONCLUSIONS: The study in hand offers a comprehensive study to clarify the bioactive metabolites of the different organs of P. pruinosa. The basic pharmacological effects and underlying mechanism of actions in the management of STZ and high fat diet induced T2DM were specifically covered in this paper.


Assuntos
Diabetes Mellitus Experimental , Diabetes Mellitus Tipo 2 , Physalis , Vitanolídeos , Animais , Citocromo P-450 CYP1A1 , Diabetes Mellitus Experimental/metabolismo , Diabetes Mellitus Tipo 2/metabolismo , Glucose/metabolismo , Hipoglicemiantes/análise , Hipoglicemiantes/farmacologia , Hipoglicemiantes/uso terapêutico , Insulina , NF-kappa B , Farmacologia em Rede , Extratos Vegetais/farmacologia , Extratos Vegetais/uso terapêutico , Proteínas Proto-Oncogênicas c-akt/metabolismo , Quercetina/uso terapêutico , Ratos , Estreptozocina , Espectrometria de Massas em Tandem , Fator de Necrose Tumoral alfa
4.
Food Res Int ; 161: 111903, 2022 11.
Artigo em Inglês | MEDLINE | ID: mdl-36192919

RESUMO

In this work, metabolic profiling of the different parts of ground cherry (P. pruinosa) including fruits, calyces, leaves, stems and roots using UPLC-MS/MS analysis combined to chemometric analysis was attempted. A total of 82 chromatographic peaks belonging to different metabolite classes were identified including terpenes, flavonoids genin and glycosides, withanolides, physalins, sucrose esters, fatty acids and other miscellaneous compounds with withanolides being the most predominant class. Roots extracts possessed the highest relative content of the identified 5ß,6ß-epoxy withanolides and intermediate withanolides, while sucrose esters and flavonoidal glycosides were found in a great abundance in calyces extracts. Moreover, physalins were found in all extracts except for roots extracts. Studying the coefficients plots revealed that terpenes and physalins (physanicantriol, loliolide, physalisitin C) were responsible for discrimination of fruits extracts. Calyces, leaves and stems extracts were found to possess antioxidant activity and higher inhibition of α-glucosidase activity. In an attempt to identify the compounds responsible for the hypoglycemic activity using both α-amylase and α-glucosidase inhibition assays, OPLS models coefficient plots were constructed which indicated that physangulide B, physaperuvin G, neophysalin A, and acylsucroses were positively correlated to α-glucosidase inhibition, while guaiacyl-primeveroside, phyperunolide C, physalactone, physalolactone C and perulactone, were positively correlated to α-amylase inhibitory activity.


Assuntos
Physalis , Vitanolídeos , Antioxidantes/química , Quimiometria , Cromatografia Líquida , Ésteres , Ácidos Graxos , Flavonoides , Glicosídeos , Hipoglicemiantes , Metabolômica/métodos , Physalis/química , Extratos Vegetais/química , Sacarose , Espectrometria de Massas em Tandem , Terpenos , Vitanolídeos/química , Vitanolídeos/farmacologia , alfa-Amilases , alfa-Glucosidases
5.
Food Funct ; 13(22): 11733-11743, 2022 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-36281695

RESUMO

Physalis peruviana L. is a common edible fruit in Egypt and other regional countries. In the present study, we investigated its crude extract as a potential source of antiproliferative secondary metabolites. Upon bioactivity guided solvent fractionation, ethyl acetate extract showed preferential activity toward the human pancreatic cancer cell line PANC-1 with an IC50 value of 5.23 ± 0.2 µg mL-1. The subsequent HR-LCMS-guided and biological activity-guided isolation revealed magnolin as a potent preferential antiproliferative agent against PANC-1 with an IC50 of 0.51 ± 0.46 µM that was comparable to that of the positive control doxorubocin (IC50 of 0.17 ± 0.15 µM). Moreover, magnolin showed much less cytotoxicity in comparison with the positive control doxorubicin (6.96% and 30.48% growth inhibition, respectively, at 5 µg mL-1) towards normal human cells (i.e. dermal fibroblasts; HDFa). Furthermore, magnolin was able to induce a concentration-dependent suppression of the formation of PANC-1 colonies, where the treatment of the tumor cells with 25 nM, 50 nM, and 100 nM concentrations of the compound resulted in a 36%, 57, and 78% reduction, respectively, in the PANC-1 colony formation. Additionally, magnolin was observed to limit PANC-1 tumor cell migration in the tumor cell wound healing assay, indicating a substantial anti-migratory effect against the PANC-1 cell line. A subsequent in silico-based study of this compound structure putatively suggested matrix metalloproteinase-3 (MMP3) as the molecular target that mediates these observed effects on PANC-1 cells. Absolute binding free energy estimation (ΔGbinding) and 100 ns long molecular dynamics simulation (MDS) experiments indicated that the magnolin structure has good affinity towards the MMP3's active site and can achieve significantly stable binding inside it. Accordingly, upon experimental validation, magnolin was found to inhibit the catalytic activity of MMP3 in a dose-dependent manner with a nanomolar IC50 value of 185 nm ± 4.86 and a Ki of 112 nm ± 6.31. In conclusion, our results clearly revealed that magnolin derived from P. peruviana is an interesting antiproliferative and antimetastatic agent against PANC-1 cells with potent inhibitory activity against MMP3. Further in vivo evaluation will be of great interest in the future.


Assuntos
Lignanas , Neoplasias Pancreáticas , Physalis , Humanos , Metaloproteinase 3 da Matriz , Lignanas/farmacologia , Neoplasias Pancreáticas/tratamento farmacológico , Linhagem Celular Tumoral , Proliferação de Células
6.
Molecules ; 27(18)2022 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-36144521

RESUMO

In recent years there has been an extensive search for nature-based products with functional potential. All structural parts of Physalis alkekengi (bladder cherry), including fruits, pulp, and less-explored parts, such as seeds and peel, can be considered sources of functional macro- and micronutrients, bioactive compounds, such as vitamins, minerals, polyphenols, and polyunsaturated fatty acids, and dietetic fiber. The chemical composition of all fruit structural parts (seeds, peel, and pulp) of two phenotypes of P. alkekengi were studied. The seeds were found to be a rich source of oil, yielding 14-17%, with abundant amounts of unsaturated fatty acids (over 88%) and tocopherols, or vitamin E (up to 5378 mg/kg dw; dry weight). The predominant fatty acid in the seed oils was linoleic acid, followed by oleic acid. The seeds contained most of the fruit's protein (16-19% dw) and fiber (6-8% dw). The peel oil differed significantly from the seed oil in fatty acid and tocopherol composition. Seed cakes, the waste after oil extraction, contained arginine and aspartic acid as the main amino acids; valine, phenylalanine, threonine, and isoleucine were present in slightly higher amounts than the other essential amino acids. They were also rich in key minerals, such as K, Mg, Fe, and Zn. From the peel and pulp fractions were extracted fruit concretes, aromatic products with specific fragrance profiles, of which volatile compositions (GC-MS) were identified. The major volatiles in peel and pulp concretes were ß-linalool, α-pinene, and γ-terpinene. The results from the investigation substantiated the potential of all the studied fruit structures as new sources of bioactive compounds that could be used as prospective sources in human and animal nutrition, while the aroma-active compounds in the concretes supported the plant's potential in perfumery and cosmetics.


Assuntos
Frutas , Physalis , Arginina/análise , Ácido Aspártico/análise , Ácidos Graxos/análise , Ácidos Graxos Insaturados/análise , Frutas/química , Humanos , Isoleucina , Ácido Linoleico/análise , Ácido Oleico/análise , Fenilalanina/análise , Physalis/química , Óleos Vegetais/química , Estudos Prospectivos , Sementes/química , Treonina , Tocoferóis/análise , Valina/análise , Vitaminas/análise
7.
J Food Sci ; 87(10): 4429-4439, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-36102046

RESUMO

Cape gooseberry fruits are increasingly recognized due to their excellent organoleptic and functional characteristics as a food. As the cultivation of this fruit expands, it is necessary to determine the quality characteristics and evolution of the new growing zones. This study sought to characterize the growth and development of cape gooseberry fruits, Ecotype Colombia, in the Ventaquemada region (Department of Boyacá in Colombia). For the experiments, 50 plants were taken completely at random from which 20 flowers of the middle third were selected and marked considering that 50% of the flowers were open. The selected cape gooseberry plants were 9 months old from the establishment. Samples were carried out every 5-7 days to evaluate changes in different physiological and physicochemical properties of the fruits such as equatorial diameter, weight, dry matter, respiration intensity (RI), total soluble solids (SST), titratable acidity (TA), and maturity ratio (SST/TA). Logistic and modified enzyme kinetics models were fitted to represent the change in these characteristics as a function of growth time and to establish a suitable maturity index. A rapid increase in the growth and a substantive change in the properties of the fruits were observed between 36 and 45 days after anthesis (DAA) and stabilization between 60 and 65 DAA, which constitutes the stage of physiological maturity. At this stage, fruits with a polar/equatorial diameter of 2.2-2.5 cm, 14.9% TSS, 2.2% TA, and 191.7 cm3 CO2 /g/d RI were obtained. Likewise, from the adjusted models, it was possible to identify these changes, especially for the relative growth rate (RGR), color index, and maturity ratio. PRACTICAL APPLICATION: In this work, we present the characterization of some physicochemical properties of cape gooseberry throughout its development and preharvest growth. This fruit is of wide interest due to its broad nutritional properties and is known as a "superfruit." The monitoring of the changes in the quality characteristics is important because it allows producers to define an optimum maturity stage for its commercialization. We also fit suitable regression models to relate the evolution in the product as a function of time and provide prediction tools to monitor fruit development. The above can help growers better schedule their production cycles.


Assuntos
Physalis , Ribes , Frutas/química , Ecótipo , Colômbia , Antivirais/análise , Dióxido de Carbono/análise , Crescimento e Desenvolvimento
8.
Molecules ; 27(13)2022 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-35807484

RESUMO

There is increasing interest in research of secondary metabolites from Physalis peruviana (Cape gooseberry) because of their potential bioactivities. In this study, the profile of compounds found in fruits and husks from Costa Rica was determined through ultra-performance liquid chromatography coupled with high-resolution mass spectrometry using a quadrupole time-of-flight analyzer (UPLC-ESI-QTOF MS) on extracts (n = 10) obtained through pressurized liquid extraction (PLE) conditions. In total, 66 different compounds were identified, comprising 34 withanolides, 23 sucrose ester derivatives and 9 flavonoids. UPLC-DAD analysis was performed to determine the ß-carotene in fruits and to quantify the flavonoids in all 10 samples, with the results showing higher contents in samples from the Dota region (58.6-60.1 µg/g of dry material versus 1.6-2.8 mg/g of dry material). The Folin-Ciocalteau total polyphenolic content (FC) and antioxidant activity using the DPPH method showed better results for the husk extracts, with the ones from the Dota region holding the best values (4.3-5.1 mg GAE/g of dry material versus IC50 = 1.6-2.3 mg of dry material/mL). In addition, a significant negative correlation was found between the RU, FC and DPPH values (r = -0.902, p < 0.05), aligning with previous reports on the role of polyphenols in antioxidant activity. Principal correlation analysis (PCoA) and hierarchical clustering (HC) analysis were performed on HRMS results, and they indicated that the D1 and D2 fruit samples from the Dota region were clustered with husks related to a higher presence of the analyzed metabolites. In turn, principal component analysis (PCA) performed on the flavonoid content and antioxidant activity yielded results indicating that the D1 and D2 husks and fruit samples from the Dota region stood out significantly, showing the highest antioxidant activity. In summation, our findings suggest that P. peruviana husks and fruits from Costa Rica constitute a substrate of interest for further studies on their potential health benefits.


Assuntos
Physalis , Ribes , Antioxidantes/química , Costa Rica , Flavonoides/química , Frutas/química , Physalis/química , Extratos Vegetais/química
9.
Spectrochim Acta A Mol Biomol Spectrosc ; 280: 121551, 2022 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-35779475

RESUMO

Treatments that allow plants to better tolerate water deficit become essential, such as the application of chemical priming. In addition, it is essential to use analyses capable of measuring these effects at the biomolecular level, complementing the other physiological evaluations. In view of the above, this study aimed to evaluate the use of attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy for analyses of water deficit tolerance in Physalis peruviana plants. For this, samples of leaves, stems and roots of plants subjected to different pretreatments with proline (10 mM and 20 mM), sodium nitroprusside (SNP 25 µM and 50 µM) and H2O as control, aiming at increasing tolerance to water deficit, were evaluated. The chemical agents used attenuated water deficit in P. peruviana plants, influencing phenotypic characterization and spectral analyses. Analysis of FTIR spectra indicates that different functional groups present in leaves, stems and roots were influenced by water deficit and priming treatments. Changes in lipid levels contributed to reducing water losses by increasing the thickness of cuticular wax. Accumulation of proteins and carbohydrates promoted osmoregulation and maintenance of the water status of plants. Thus, water deficit causes changes in the functional groups present in the organs of P. peruviana, and the ATR-FTIR technique is able to detect these biomolecular changes, helping in the selection of priming treatments to increase tolerance to water deficit.


Assuntos
Physalis , Água , Análise de Fourier , Folhas de Planta , Plantas , Espectroscopia de Infravermelho com Transformada de Fourier/métodos
10.
Phytochemistry ; 202: 113301, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-35780926

RESUMO

Eight undescribed and two known withanolides were obtained from the aerial parts of Physalis minima. The structures of these compounds were defined by spectroscopic methods including 1D and 2D NMR, HRESIMS, and electronic circular dichroism (ECD) data analysis. Physminin E was elucidated to be a rare 13,14-seco-withanolide. Inhibitory effects of these compounds on nitric oxide (NO) production were evaluated by using LPS-activated RAW264.7 macrophages, and physminin C was shown to be the most active with an IC50 value of 3.5 µM. The further mechanistic analysis of NO inhibition was performed by molecular docking and Western blotting.


Assuntos
Physalis , Vitanolídeos , Anti-Inflamatórios/química , Anti-Inflamatórios/farmacologia , Simulação de Acoplamento Molecular , Estrutura Molecular , Óxido Nítrico , Physalis/química , Componentes Aéreos da Planta , Vitanolídeos/química , Vitanolídeos/farmacologia
11.
Phytomedicine ; 105: 154328, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-35908519

RESUMO

BACKGROUND: Physalis alkekengi L. var. franchetii (Mast.) Makino (PAF) (Chinese name Jin-Deng-Long) from the Solanaceae family is a traditional Chinese medicine with various pharmacological effects, such as removing heat, detoxification, improving throat conditions, removing phlegm, and ameliorating diuresis. PURPOSE: This paper reviews the existing literature and patents and puts forward some suggestions for future PAF research. METHODS: Using the PubMed, Google Scholar, Web of Science, and China National Knowledge Infrastructure databases, we performed comprehensive search of literature and patents published before April 2022 on PAF and its active ingredients. RESULTS: We comprehensively reviewed the research progress of PAF from aspects of the traditional application, botany, chemical composition, pharmacological effects, and toxicology, and first discussed quality control and modern applications, which have not been explored in previous reviews. Thereafter, we reviewed the limitations of pharmacological mechanism and quality control studies and proposed appropriate solutions, which is of great practical significance to subsequent studies. CONCLUSION: In this review, we present a comprehensive overview on PAF, and put forward new insights on studies regarding quality control, material basis, and mechanisms in classical prescription, providing theoretical guidance for the clinical application and development of Chinese medicine.


Assuntos
Physalis , China , Medicina Tradicional Chinesa , Farmacognosia , Compostos Fitoquímicos , Controle de Qualidade
12.
Acta Virol ; 66(2): 174-181, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35766474

RESUMO

Tamarillo leaf malformation virus (TaLMV) is a potyvirus first discovered in cape gooseberry fields in Eastern, and South-western Antioquia. This virus is responsible for a very damaging disease that has resulted in significant reductions in yields and cultivated area for this crop in Colombia. Tamarillo is frequently co-cultivated with other solanaceous plants but no evidence for cross-pathogenicity of TaLMV has been found until now. In this work, we report a natural infection of cape gooseberry (Physalis peruviana L.) by TaLMV. Infection by TaLMV was detected by RNAseq screening of cape gooseberry fields and confirmed by RT-qPCR and Sanger sequencing. The sequenced genome is 99.3% identical to previously sequenced TaLMV isolates, and evidence suggests that it can accumulate at high loads in this new reported host. RT-qPCR analysis indicates that TaLMV is already widely distributed, can naturally infect other solanaceous hosts and may become an emerging threat to the cape gooseberry agroindustry, the second most important exotic fruit export in Colombia. Keywords: high-throughput sequencing; plant virology; Potyviridae; RT-qPCR; Solanaceae.


Assuntos
Physalis , Potyvirus , Ribes , Physalis/genética , Doenças das Plantas , Folhas de Planta , Potyvirus/genética
13.
Braz J Biol ; 84: e258128, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35649038

RESUMO

In the western mountainous region of Khyber Pakhtunkhwa Pakistan at the Shangla district, we found Physalis ixocarpa for the first time, not yet reported from Pakistan. Physalis ixocarpa was unidentified and has no ethnobotanical record in the flora of Pakistan. It is a member of family Solanaceae and having a close relation with Solanum tuberosum and Lycopersicon esculentum. The stem is prostrate with a dichotomous pattern of branches having leaves flower and fruits. Leaves are smooth, ovate and the margins of leaf blade dentation are poorly developed. The average length and width of the leaves are 6.50 and 3.61 cm respectively. P. ixocarpa grows to the length of 4-5 feet and an annual herb. The flowers of the plants are yellow in color and having purple color spots on the petals which are star-shaped. The round berry fruits are surrounded by persistent calyx and purple in color. The fruits are the 3-6cm in diameter. The plants are found in the different localities of district Shangla especially in Bar and Koz Kana. The life cycle of reporting plant is started in May and completed in November.


Assuntos
Physalis , Etnobotânica , Paquistão , Folhas de Planta , Plantas
14.
J Nat Prod ; 85(6): 1522-1539, 2022 06 24.
Artigo em Inglês | MEDLINE | ID: mdl-35608269

RESUMO

The inherent structural instability of some physalins has hampered the isolation and identification of these compounds for approximately 50 years, and an effective method to overcome these challenges remains unavailable. In the present study, the unprecedented tautomerization mechanism of unstable physalins was elucidated by performing isotopic labeling experiments and DFT calculations, which led to the successful separation of tautomers and isolation of highly pure products for the first time. As a result, 15 new physalins, physaminins A-O (1-15), as well as 17 known analogues (16-32), were isolated from the whole plants of Physalis minima L. The chemical structures of the new compounds were established by performing a comprehensive analysis of spectroscopic data, and their absolute configurations were confirmed by using computational ECD calculations and/or single-crystal X-ray diffraction analyses. All obtained isolates were evaluated for their antiproliferative effects against four human cancer cell lines (A549, HepG2, MCF-7, and SCG-7901) and two noncancerous cell lines (RAW 264.7 and human normal hepatocytes L02), as well as their anti-inflammatory activities by measuring their abilities to inhibit NO production in LPS-stimulated murine RAW 264.7 cells in vitro. Compounds 1-5, 13, 16, 18, 19, 23, and 30 exerted significant antiproliferative effects on the four human cancer lines, with IC50 values ranging from 0.2(0) to 24.7(2) µM, and these compounds were not toxic to the two noncancerous cell lines at a concentration of 10 µM. Moreover, compounds 7, 10, 11, 12, 14, 17, 22, and 27 significantly inhibited NO production, with IC50 values ranging from 2.9(1) to 9.5(2) µM.


Assuntos
Physalis , Animais , Anti-Inflamatórios/farmacologia , Humanos , Camundongos , Estrutura Molecular , Physalis/química , Células RAW 264.7
15.
J Agric Food Chem ; 70(18): 5595-5609, 2022 May 11.
Artigo em Inglês | MEDLINE | ID: mdl-35476903

RESUMO

Physalis minima is a medicinal and edible plant in China. In this study, 22 new withaphysalins, including a novel 1(10 → 6)abeo-14ß-hydroxy one (1) and other 15 unusual 14ß-hydroxy ones (3-4, 6-17, 19), were isolated from the whole herbs of P. minima together with two known analogues (23-24). Their structures were established by extensive analysis of high-resolution electrospray ionization mass spectrometry, IR, and 1D and 2D NMR spectroscopic data. Their absolute configurations were determined by electronic circular dichroism (ECD) spectra and single-crystal X-ray crystallographic analyses, together with DFT NMR calculations. All isolated compounds were evaluated for their anti-inflammatory activity via measuring the colorimetric reporter of the secreted embryonic alkaline phosphatase gene driven by an IFN-ß minimal promoter fused to five copies of the NF-κB consensus transcriptional response element and three copies of the c-Rel binding site in LPS-stimulated human THP1-Dual cells. Compounds 2, 5, 6, 9, 10, 11, and 20 showed significant anti-inflammatory effects with IC50 values in the range of 3.01-13.39 µM. Among them, compounds 2 and 10 showed better anti-inflammatory effects to inhibit the secretion of IL-6, IL-1ß, and TNF-α in LPS-stimulated THP1-Dual cells.


Assuntos
Physalis , Vitanolídeos , Anti-Inflamatórios/química , Anti-Inflamatórios/farmacologia , Humanos , Lipopolissacarídeos/farmacologia , NF-kappa B , Physalis/química , Vitanolídeos/química , Vitanolídeos/farmacologia
16.
Plant J ; 111(1): 183-204, 2022 07.
Artigo em Inglês | MEDLINE | ID: mdl-35481627

RESUMO

Solanaceae have important economic value mainly due to their edible fruits. Physalis organ size 1/cytokinin response factor 3 (POS1/CRF3), a unique gene in Solanaceae, is involved in fruit size variation in Physalis but not in Solanum. However, the underlying mechanisms remain elusive. Here, we found that POS1/CRF3 was likely created via the fusion of CRF7 and CRF8 duplicates. Multiple genetic manipulations revealed that only POS1 and Capsicum POS1 (CaPOS1) functioned in fruit size control via the positive regulation of cell expansion. Comparative studies in a phylogenetic framework showed the directional enhancement of POS1-like expression in the flowers and fruits of Physaleae and the specific gain of certain interacting proteins associated with cell expansion by POS1 and CaPOS1. A lineage-specific single nucleotide polymorphism (SNP) caused the 68th amino acid histidine in the POS1 orthologs of non-Physaleae (Nicotiana and Solanum) to change to arginine in Physaleae (Physalis and Capsicum). Substituting the arginine in Physaleae POS1-like by histidine completely abolished their function in the fruits and the protein-protein interaction (PPI) with calreticulin-3. Transcriptomic comparison revealed the potential downstream pathways of POS1, including the brassinosteroid biosynthesis pathway. However, POS1-like may have functioned ancestrally in abiotic stress within Solanaceae. Our work demonstrated that heterometric expression and a SNP caused a single amino acid change to establish new PPIs, which contributed to the co-option of POS1 in multiple regulatory pathways to regulate cell expansion and thus fruit size in Physaleae. These results provide new insights into fruit morphological evolution and fruit yield control.


Assuntos
Capsicum , Physalis , Solanaceae , Arginina/metabolismo , Capsicum/genética , Citocininas/metabolismo , Frutas/genética , Frutas/metabolismo , Regulação da Expressão Gênica de Plantas/genética , Histidina/genética , Histidina/metabolismo , Filogenia , Physalis/genética , Physalis/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Solanaceae/genética
17.
Molecules ; 27(5)2022 Feb 22.
Artigo em Inglês | MEDLINE | ID: mdl-35268579

RESUMO

Physalis angulata L. belongs to the family Solanaceae and is distributed throughout the tropical and subtropical regions. Physalis angulata leaf and fruit extracts were assessed for in vitro anticancer, antioxidant activity, and total phenolic and flavonoid content. The GC-MS technique investigated the chemical composition and structure of bioactive chemicals reported in extracts. The anticancer activity results revealed a decrease in the percentage of anticancer cells' viability in a concentration- and time-dependent way. We also noticed morphological alterations in the cells, which we believe are related to Physalis angulata extracts. Under light microscopy, we observed that as the concentration of ethanolic extract (fruit and leaves) treated HeLa cells increased, the number of cells began to decrease.


Assuntos
Physalis
18.
Front Biosci (Landmark Ed) ; 27(3): 98, 2022 03 15.
Artigo em Inglês | MEDLINE | ID: mdl-35345330

RESUMO

BACKGROUND: Many drugs for anti-tumour have been developed, nevertheless, seeking new anticancer drug is the focus of ongoing investigation. Withanolides have been reported to possess potent antiproliferative activity. Literature findings revealed that a diversity of withanolides were obtained from Physalis peruviana, however, the antitumor activity of these bioactive compounds is still unclear. METHODS: The EtOAc fraction of P. peruviana were decolorized on Middle Chromatogram Isolated (MCI) Gel column, repeatedly subjected to column chromatography (CC) over sephadex LH-20, preparative High Performance Liquid Chromatography (HPLC) and silica gel to afford compounds. Their chemical structures of the new isolates were elucidated through analyzing spectroscopic and HRESIMS data. All these obtained metabolites were appraised for their potential antiproliferative activity against the human breast cancer cell line MCF-7 by MTT assay, and in vitro antibacterial activity of the isolated compounds (1-7) were evaluated against E. coli, B. cereus and S. aureus. Results: Four new withanolides, including one withaphysalin-type withanolide (peruranolide A, 1), two 13,14-seco-withaphysalins (peruranolides B-C, 2-3), as well as one normal withanolide (peruranolide D, 4), were purified and separated from P. peruviana L.. Compound 5 was discovered to exhibit potent cytotoxic effect with an IC50 value of 3.51 µM. In vitro antibacterial activities, compounds 1-7 had no obvious inhibitory activity against E. coli, but had moderate inhibitory activities against B. cereus and S. aureus. CONCLUSIONS: Our findings might offer valuable clues for the utilization of withanolides as lead compounds for antineoplastic or antibacterial drug development.


Assuntos
Antineoplásicos , Physalis , Vitanolídeos , Antibacterianos/farmacologia , Antineoplásicos/farmacologia , Escherichia coli , Humanos , Estrutura Molecular , Physalis/química , Staphylococcus aureus , Vitanolídeos/farmacologia
19.
Molecules ; 27(3)2022 Jan 21.
Artigo em Inglês | MEDLINE | ID: mdl-35163960

RESUMO

The calyxes and fruits of Physalis alkekengi L. var. franchetii (Mast.) Makino (P. alkekengi), a medicinal and edible plant, are frequently used as heat-clearing and detoxifying agents in thousands of Chinese medicine prescriptions. For thousands of years in China, they have been widely used in clinical practice to treat throat disease, hepatitis, and bacillary dysentery. This systematic review summarizes their structural analysis, quality control, pharmacology, and pharmacokinetics. Furthermore, the possible development trends and perspectives for future research studies on this medicinal plant are discussed. Relevant information on the calyxes and fruits of P. alkekengi was collected from electronic databases, Chinese herbal classics, and Chinese Pharmacopoeia. Moreover, information was collected from ancient documents in China. The components isolated and identified in P. alkekengi include steroids, flavonoids, phenylpropanoids, alkaloids, nucleosides, terpenoids, megastigmane, aliphatic derivatives, organic acids, coumarins, and sucrose esters. Steroids, particularly physalins and flavonoids, are the major characteristic and bioactive ingredients in P. alkekengi. According to the literature, physalins are synthesized by the mevalonate and 2-C-methyl-d-erythritol-4-phosphate pathways, and flavonoids are synthesized by the phenylpropanoid pathway. Since the chemical components and pharmacological effects of P. alkekengi are complex and varied, there are different standards for the evaluation of its quality and efficacy. In most cases, the analysis was performed using high-performance liquid chromatography coupled with ultraviolet detection. A pharmacological study showed that the crude extracts and isolated compounds from P. alkekengi had extensive in vitro and in vivo biological activities (e.g., anti-inflammatory, anti-tumor, immunosuppressive, antibacterial, anti-leishmanial, anti-asthmatic, anti-diabetic, anti-oxidative, anti-malarial, anti-Alzheimer's disease, and vasodilatory). Moreover, the relevant anti-inflammatory and anti-tumor mechanisms were elucidated. The reported activities indicate the great pharmacological potential of P. alkekengi. Similarly, studies on the pharmacokinetics of specific compounds will also contribute to the progress of clinical research in this setting.


Assuntos
Produtos Biológicos/análise , Physalis/enzimologia , Physalis/metabolismo , Produtos Biológicos/farmacologia , China , Cromatografia Líquida de Alta Pressão/métodos , Ésteres/química , Flavonoides , Flores/efeitos dos fármacos , Frutas/efeitos dos fármacos , Physalis/química , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Sacarose/química
20.
Molecules ; 27(3)2022 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-35164184

RESUMO

Withanolides constitute one of the most interesting classes of natural products due to their diversity of structures and biological activities. Our recent studies on withanolides obtained from plants of Solanaceae including Withania somnifera and a number of Physalis species grown under environmentally controlled aeroponic conditions suggested that this technique is a convenient, reproducible, and superior method for their production and structural diversification. Investigation of aeroponically grown Physalis coztomatl afforded 29 withanolides compared to a total of 13 obtained previously from the wild-crafted plant and included 12 new withanolides, physacoztolides I-M (9-13), 15α-acetoxy-28-hydroxyphysachenolide C (14), 28-oxophysachenolide C (15), and 28-hydroxyphysachenolide C (16), 5α-chloro-6ß-hydroxy-5,6-dihydrophysachenolide D (17), 15α-acetoxy-5α-chloro-6ß-hydroxy-5,6-dihydrophysachenolide D (18), 28-hydroxy-5α-chloro-6ß-hydroxy-5,6-dihydrophysachenolide D (19), physachenolide A-5-methyl ether (20), and 17 known withanolides 3-5, 8, and 21-33. The structures of 9-20 were elucidated by the analysis of their spectroscopic data and the known withanolides 3-5, 8, and 21-33 were identified by comparison of their spectroscopic data with those reported. Evaluation against a panel of prostate cancer (LNCaP, VCaP, DU-145, and PC-3) and renal carcinoma (ACHN) cell lines, and normal human foreskin fibroblast (WI-38) cells revealed that 8, 13, 15, and 17-19 had potent and selective activity for prostate cancer cell lines. Facile conversion of the 5,6-chlorohydrin 17 to its 5,6-epoxide 8 in cell culture medium used for the bioassay suggested that the cytotoxic activities observed for 17-19 may be due to in situ formation of their corresponding 5ß,6ß-epoxides, 8, 27, and 28.


Assuntos
Antineoplásicos Fitogênicos/metabolismo , Antineoplásicos Fitogênicos/farmacologia , Physalis/crescimento & desenvolvimento , Vitanolídeos/metabolismo , Vitanolídeos/farmacologia , Antineoplásicos Fitogênicos/química , Vias Biossintéticas , Biotecnologia , Linhagem Celular Tumoral , Humanos , Masculino , Physalis/química , Physalis/metabolismo , Neoplasias da Próstata/tratamento farmacológico , Vitanolídeos/química
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