Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 50.337
Filtrar
Mais filtros








Intervalo de ano de publicação
1.
Braz. j. biol ; 83: e242603, 2023. tab, graf
Artigo em Inglês | MEDLINE, LILACS, VETINDEX | ID: biblio-1355852

RESUMO

Abstract Transcription factors (TF) are a wide class of genes in plants, and these can regulate the expression of other genes in response to various environmental stresses (biotic and abiotic). In the current study, transcription factor activity in sugarcane was examined during cold stress. Initially, RNA transcript reads of two sugarcane cultivars (ROC22 and GT08-1108) under cold stress were downloaded from SRA NCBI database. The reads were aligned into a reference genome and the differential expression analyses were performed with the R/Bioconductor edgeR package. Based on our analyses in the ROC22 cultivar, 963 TF genes were significantly upregulated under cold stress among a total of 5649 upregulated genes, while 293 TF genes were downregulated among a total of 3,289 downregulated genes. In the GT08-1108 cultivar, 974 TF genes were identified among 5,649 upregulated genes and 283 TF genes were found among 3,289 downregulated genes. Most transcription factors were annotated with GO categories related to protein binding, transcription factor binding, DNA-sequence-specific binding, transcription factor complex, transcription factor activity in RNA polymerase II, the activity of nucleic acid binding transcription factor, transcription corepressor activity, sequence-specific regulatory region, the activity of transcription factor of RNA polymerase II, transcription factor cofactor activity, transcription factor activity from plastid promoter, transcription factor activity from RNA polymerase I promoter, polymerase II and RNA polymerase III. The findings of above results will help to identify differentially expressed transcription factors during cold stress. It also provides a comprehensive analysis of the regulation of the transcription activity of many genes. Therefore, this study provides the molecular basis for improving cold tolerance in sugarcane and other economically important grasses.


Resumo Fatores de transcrição (FT) são uma ampla classe de genes em plantas e podem regular a expressão de outros genes em resposta a vários estresses ambientais (estresses bióticos e abióticos). No presente estudo, a atividade do fator de transcrição na cana-de-açúcar foi examinada durante o estresse pelo frio. Inicialmente, as leituras de transcrição de RNA de duas cultivares de cana-de-açúcar (ROC22 e GT08-1108) sob estresse frio foram baixadas do banco de dados SRA NCBI. As leituras foram alinhadas em um genoma de referência e as análises de expressão diferencial foram realizadas com o pacote R / Bioconductor edgeR. Com base em nossas análises no cultivar ROC22, 963 genes TF foram significativamente regulados positivamente sob estresse pelo frio entre um total de 5.649 genes regulados positivamente, enquanto 293 genes TF foram regulados negativamente entre um total de 3.289 genes regulados negativamente. No cultivar GT08-1108, 974 genes TF foram identificados entre 5.649 genes regulados positivamente e 283 genes TF foram encontrados entre 3.289 genes regulados negativamente. Os fatores de transcrição, em sua maioria, foram anotados com categorias GO relacionadas à ligação de proteína, ligação de fator de transcrição, ligação específica de sequência de DNA, complexo de fator de transcrição, atividade de fator de transcrição em RNA polimerase II, atividade de fator de transcrição de ligação de ácido nucleico, atividade de corepressor de transcrição, sequência específica da região reguladora, atividade do fator de transcrição da RNA polimerase II, atividade do cofator do fator de transcrição, atividade do fator de transcrição do promotor do plastídio, atividade do fator de transcrição do promotor da RNA polimerase I, polimerase II e RNA polimerase III. As descobertas dos resultados acima ajudarão a identificar fatores de transcrição expressos diferencialmente durante o estresse pelo frio. Ele também fornece uma análise abrangente da regulação da atividade de transcrição de muitos genes. Portanto, este estudo fornece base molecular para melhorar a tolerância ao frio em cana-de-açúcar e outras gramíneas economicamente importantes.


Assuntos
Saccharum/genética , Saccharum/metabolismo , Resposta ao Choque Frio/genética , Estresse Fisiológico/genética , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo , Temperatura Baixa , Regulação da Expressão Gênica de Plantas , Perfilação da Expressão Gênica
2.
Sheng Li Xue Bao ; 74(2): 201-208, 2022 Apr 25.
Artigo em Chinês | MEDLINE | ID: mdl-35503067

RESUMO

The shivering and nonshivering thermogenesis in skeletal muscles is important for maintaining body temperature in a cold environment. In addition to nervous-humoral regulation, adipose tissue was demonstrated to directly respond to cold in a cell-autonomous manner to produce heat. However, whether skeletal muscle can directly respond to low temperature in an autoregulatory manner is unknown. Transient receptor potential (TRP) channels TRPM8 and TRPA1 are two important cold sensors. In the current study, we found TRPM8 was expressed in mouse skeletal muscle tissue and C2C12 myotubes by RT-PCR. After exposure to 33 °C for 6 h, the gene expression pattern of C2C12 myotubes was significantly changed which was evidenced by RNA-sequencing. KEGG-Pathway enrichment analysis of these differentially expressed genes showed that low temperature changed several important signaling pathways, such as IL-17, TNFα, MAPK, FoxO, Hedgehog, Hippo, Toll-like receptor, Notch, and Wnt signaling pathways. Protein-protein interaction network analysis revealed that IL-6 gene was a key gene which was directly affected by low temperature in skeletal muscle cells. In addition, both mRNA and protein levels of IL-6 were increased by 33 °C exposure in C2C12 myotubes. In conclusion, our findings demonstrated that skeletal muscle cells could directly respond to low temperature, characterized by upregulated expression of IL-6 in skeletal muscle cells.


Assuntos
Temperatura Baixa , Interleucina-6 , Animais , Interleucina-6/genética , Interleucina-6/metabolismo , Camundongos , Fibras Musculares Esqueléticas/metabolismo , Músculo Esquelético/fisiologia , Temperatura
3.
Biol Lett ; 18(5): 20220050, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35506240

RESUMO

Freezing temperatures are inherently challenging for life, which is water based. How species cope with these conditions fundamentally shapes ecological and evolutionary processes. Despite this, there is no comprehensive conceptual framework for cold-survival strategies-seasonal migration, cold resistance and torpor. Here, I propose a framework with four components for conceptualizing and quantifying cold-survival strategies. Cold-survival strategies are (i) collectively encompassed by the proposed framework, and that this full breadth of strategies should be considered in focal species or systems (comprehensive consideration). These strategies also (ii) exist on a spectrum, such that species can exhibit partial use of strategies, (iii) are non-exclusive, such that some species use multiple strategies concurrently (combined use) and (iv) should collectively vary inversely and proportionally with one another when controlling for the external environment (e.g. when considering species that occur in sympatry in their summer range), such that use of one strategy reduces, collectively, the use of others (proportional use). This framework is relevant to understanding fundamental patterns and processes in evolution, ecology, physiology and conservation biology.


Assuntos
Torpor , Temperatura Baixa , Estações do Ano , Água
4.
Methods Mol Biol ; 2469: 145-154, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35508836

RESUMO

The localization of metabolites in plant tissues is often related to their biological function and biosynthesis. Mass spectrometry imaging (MSI) provides comprehensive information about the distribution of known and unknown compounds in tissues. In this protocol, we describe the use of laser desorption low-temperature plasma (LD-LTP) ionization MSI. This technology enables the direct analysis of native tissues under ambient conditions.


Assuntos
Lasers , Plantas , Temperatura Baixa , Espectrometria de Massas/métodos , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos , Temperatura
5.
J Bodyw Mov Ther ; 30: 100-104, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-35500956

RESUMO

INTRODUCTION: Cold spray is a form of cryotherapy used in acute injuries at sports medicine. In the current study, we aimed to investigate the acute effect of cold spray application as a treatment strategy for acute sports injuries on the mechanical properties of the rectus femoris muscle in athletes. METHODS: The study included 23 volunteer active male athletes between the ages of 18-23 with a subepidermal fold thickness of the rectus femoris muscle between 5 mm and 15 mm. Skin temperature (thermal camera) and mechanical property evaluations of the muscle (Myoton Pro) were measured before, immediately after, and in 2 min, 5 min, 10 min and 15 min intervals following cold spray application. RESULTS: The skin temperature value of the athletes were significantly lower even in the 15-min interval following application (p < 0.001). Muscle tone increased significantly after the application and in the second minute compared to the pre-application (p < 0.001). Muscle stiffness increased significantly only after the second and fifth minutes compared to the pre-application (p < 0.001). Muscle decrement showed an increase in all time-points compared to the pre-application (p < 0.05). CONCLUSION: In athletes, the rectus femoris muscle has been observed to become harder and less elastic following cooling with cold spray. These changes did not improve completely following the 5 min. The muscle regained its mechanical properties at the earliest approximately 10 min after the cold spray application.


Assuntos
Traumatismos em Atletas , Músculo Quadríceps , Adolescente , Adulto , Atletas , Traumatismos em Atletas/terapia , Temperatura Baixa , Crioterapia , Humanos , Masculino , Músculo Quadríceps/fisiologia , Adulto Jovem
6.
Am J Hum Genet ; 109(5): 967-972, 2022 May 05.
Artigo em Inglês | MEDLINE | ID: mdl-35523147

RESUMO

The common loss-of-function mutation R577X in the structural muscle protein ACTN3 emerged as a potential target of positive selection from early studies and has been the focus of insightful physiological work suggesting a significant impact on muscle metabolism. Adaptation to cold climates has been proposed as a key adaptive mechanism explaining its global allele frequency patterns. Here, we re-examine this hypothesis analyzing modern (n = 3,626) and ancient (n = 1,651) genomic data by using allele-frequency as well as haplotype homozygosity-based methods. The presented results are more consistent with genetic drift rather than selection in cold climates as the main driver of the ACTN3 R577X frequency distribution in human populations across the world. This Matters Arising paper is in response to Wyckelsma et al. (2021),1 published in The American Journal of Human Genetics. See also the response by Wyckelsma et al. (2022),2 published in this issue.


Assuntos
Actinina , Músculo Esquelético , Actinina/genética , Temperatura Baixa , Frequência do Gene , Homozigoto , Humanos , Músculo Esquelético/metabolismo , Termogênese
7.
Proc Natl Acad Sci U S A ; 119(19): e2112250119, 2022 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-35500120

RESUMO

SignificanceBetter knowledge of dormancy (plant "hibernation") is required to understand future adaptation of woody perennial plants facing warmer climates. Typical dormancy research uses time to budbreak to define the transition from a warm temperature nonresponsive to a responsive state (long vs. short time to budbreak). Based on this phenotyping method, species diverge in dormancy transition times during winter. Here, dynamics of bud cold hardiness (lowest survival temperature) for many species are used to show convergence in their response to winter chilling. Therefore, previous studies determining chilling requirement based on budbreak may describe adaptation to an environment but do not accurately describe physiological dormancy transitions. Further, cold hardiness dynamics can be used for field predictions of bud cold hardiness and budbreak.


Assuntos
Aclimatação , Temperatura Baixa , Fenômenos Fisiológicos Vegetais , Clima , Estações do Ano , Temperatura
8.
Planta ; 255(5): 97, 2022 Apr 05.
Artigo em Inglês | MEDLINE | ID: mdl-35380306

RESUMO

MAIN CONCLUSION: Tuber-omics in potato with the T- and D-types of cytoplasm showed different sets of differentially expressed genes and proteins in response to cold storage. For the first time, we report differences in gene and protein expression in potato (Solanum tuberosum L.) tubers possessing the T- or D-type cytoplasm. Two F1 diploid reciprocal populations, referred to as T and D, were used. The pooling strategy was applied for detection of differentially expressed genes (DEGs) and differentially expressed proteins (DEPs) in tubers consisting of extreme chip colour after cold storage. RNA and protein bulks were constructed from contrasting phenotypes. We recognized 48 and 15 DEGs for the T and D progenies, respectively. DEPs were identified in the amyloplast and mitochondrial fractions. In the T-type cytoplasm, only 2 amyloplast-associated and 5 mitochondria-associated DEPs were detected. Of 37 mitochondria-associated DEPs in the D-type cytoplasm, there were 36 downregulated DEPs in the dark chip colour bulks. These findings suggest that T- and D-type of cytoplasm might influence sugar accumulation in cold-stored potato tubers in different ways. We showed that the mt/nucDNA ratio was higher in D-possessing tubers after cold storage than in T progeny. For the D-type cytoplasm, the pt/nucDNA ratio was higher for tubers characterized by dark chip colour than for those with light chip colour. Our findings suggest that T- and D-type cytoplasm might influence sugar accumulation in cold-stored potato tubers in different ways.


Assuntos
Solanum tuberosum , Temperatura Baixa , Citoplasma/genética , Regulação da Expressão Gênica de Plantas , Tubérculos/genética , Tubérculos/metabolismo , Proteômica , Solanum tuberosum/genética , Solanum tuberosum/metabolismo , Transcriptoma
9.
Molecules ; 27(8)2022 Apr 11.
Artigo em Inglês | MEDLINE | ID: mdl-35458659

RESUMO

Polar compounds and polymers are regarded as the most reliable indicators of oil degradation during heating, and it is desirable to find methods to reduce these undesirable changes. The aim of this study was (1) to determine the effect of enrichment with black cumin cold-pressed oil (CP) or essential oil obtained from black cumin cold-pressed oil in an equivalent amount (ES) on limiting the polar compounds and polymers content in blends based on refined rapeseed oil during high-temperature heating in a thin layer; (2) to determine tocochromanol losses and their effect on the change content of the polar compounds and polymers. Four fortified oils were made from refined rapeseed oil and one of the four additives (10% CP, 20% CP, 0.1% ES, and 0.2% ES). All fortified oils and refined rapeseed oil as a control sample were heated at 170 and 200 °C on the pan in a thin layer and evaluated regarding loss of individual tocochromanol homologs by HPLC-FL, polar compounds content, oxidized triacylglycerols (TAG), and polymers content by HPSEC-ELSD. Additionally, the fatty acid profile in nonheated oil was investigated. Tocochromanol analysis showed loss in all the samples. At 170 °C polymers were not detected; no difference was noted for polar compounds and oxidized TAG content; only the 20% CP sample showed a higher level. At 200 °C the 10% CP sample exhibited a significant protective effect with the lowest content of polar compounds, oxidized TAG, and dimers.


Assuntos
Nigella sativa , Temperatura Baixa , Calefação , Óleos Vegetais , Polímeros , Óleo de Brassica napus
10.
Environ Health Perspect ; 130(4): 47004, 2022 04.
Artigo em Inglês | MEDLINE | ID: mdl-35394808

RESUMO

BACKGROUND: Although substantial evidence suggests that high and low temperatures are adversely associated with nonaccidental mortality, few studies have focused on exploring the risks of temperature on external causes of death. OBJECTIVES: We investigated the short-term associations between temperature and external causes of death and four specific categories (suicide, transport, falls, and drowning) in 47 prefectures of Japan from 1979 to 2015. METHODS: We conducted a two-stage meta-regression analysis. First, we performed time-stratified case-crossover analyses with a distributed lag nonlinear model to examine the association between temperature and mortality due to external causes for each prefecture. We then used a multivariate meta-regression model to combine the association estimates across all prefectures in Japan. In addition, we performed stratified analyses for the associations by sex and age. RESULTS: A total of 2,416,707 external causes of death were included in the study. We found a J-shaped exposure-response curve for all external causes of death, in which the risks increased for mild cold temperatures [20th percentile; relative risk (RR)=1.09 (95% confidence interval [CI]: 1.05,1.12)] and extreme heat [99th percentile; RR=1.24 (95% CI: 1.20, 1.29)] compared with those for minimum mortality temperature (MMT). However, the shapes of the exposure-response curves varied according to four subcategories. The risks of suicide and transport monotonically increased as temperature increased, with RRs of 1.35 (95% CI: 1.26, 1.45) and 1.60 (95% CI: 1.35, 1.90), respectively, for heat, whereas J- and U-shaped curves were observed for falls and drowning, with RRs of 1.14 (95% CI: 1.03, 1.26) and 1.95 (95% CI: 1.70, 2.23) for heat and 1.13 (95% CI: 1.02, 1.26) and 2.33 (95% CI: 1.89, 2.88) for cold, respectively, compared with those for cause-specific MMTs. The sex- and age-specific associations varied considerably depending on the specific causes. DISCUSSION: Both low and high temperatures may be important drivers of increased risk of external causes of death. We suggest that preventive measures against external causes of death should be considered in adaptation policies. https://doi.org/10.1289/EHP9943.


Assuntos
Afogamento , Causas de Morte , Temperatura Baixa , Temperatura Alta , Humanos , Japão/epidemiologia , Temperatura
11.
J Physiol Anthropol ; 41(1): 17, 2022 Apr 20.
Artigo em Inglês | MEDLINE | ID: mdl-35443705

RESUMO

INTRODUCTION: Interest in human physiological responses to cold stress have seen a resurgence in recent years with a focus on brown adipose tissue (BAT), a mitochondria dense fat specialized for heat production. However, a majority of the work examining BAT has been conducted among temperate climate populations. METHODS: To expand our understanding of BAT thermogenesis in a cold climate population, we measured, using indirect calorimetry and thermal imaging, metabolic rate and body surface temperatures of BAT-positive and BAT-negative regions at room temperature, and mild cold exposure of resting participants from a small sample of reindeer herders (N = 22, 6 females) from sub-Arctic Finland. RESULTS: We found that most herders experienced a significant mean 8.7% increase in metabolic rates, preferentially metabolized fatty acids, and maintained relatively warmer body surface temperatures at the supraclavicular region (known BAT location) compared to the sternum, which has no associated BAT. These results indicate that the herders in this sample exhibit active BAT thermogenesis in response to mild cold exposure. CONCLUSIONS: This study adds to the rapidly growing body of work looking at the physiological and thermoregulatory significance of BAT and the important role it may play among cold stressed populations.


Assuntos
Rena , Tecido Adiposo Marrom/metabolismo , Animais , Temperatura Baixa , Feminino , Finlândia , Humanos , Masculino , Termogênese/fisiologia
12.
Ann Med ; 54(1): 1118-1125, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-35443846

RESUMO

BACKGROUND: Insulin is temperature sensitive as high temperatures reduce its potency. Refrigeration for insulin storage is still needed but households in remote areas do not have refrigerators. Also, the electricity supply is usually affected by natural disasters. We aim to examine the temperature-reducing efficacy of cooling devices in hot-humid conditions. METHODS: Five cooling devices, (1) earthen jar filled with water, (2) earthen jar filled with soil, (3) two clay pots, gap filled with wet soil, (4) two clay pots, gap filled with wet sand, and (5) commercial cooling wallet were used in this study. External and internal temperatures were monitored by the temperature logger between October 2019 and September 2020 in Narathiwat, Thailand. Cooling efficacy was assessed by average absolute temperature reduction and relative cooling effect. RESULTS: Mean external temperature and humidity were 27.3 ± 1.5 °C and 78.2 ± 7.1%RH. The mean differences between the external and internal temperatures were; device (1) -0.1 ± 0.6 °C (p = NS), (2) 0.0 ± 0.8 °C (p = NS), (3) -1.7 ± 0.9 °C (p < .0001), (4) -2.0 ± 0.9 °C (p < .0001), and (5) -1.8 ± 0.9 °C (p < .0001). Device no. 3, 4, and 5 achieved a constant temperature reduction. The most efficacious device was device no. 4 with a relative cooling effect of 63.6% better than the cooling wallet (57.7%, p = .003). All devices were more efficacious at lower humidity levels. CONCLUSIONS: Traditional low-cost devices, such as clay pots, reduce storage temperatures to or close to room temperature in hot-humid climates. This study provides some guidance for insulin storage in hot-humid environments.


Assuntos
Insulina , Refrigeração , Argila , Temperatura Baixa , Humanos , Umidade
13.
J Plant Physiol ; 272: 153693, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35413569

RESUMO

Genes play regulatory roles in plants' response to low-temperature stress. Our understanding of the mechanism of plants' response to low-temperature stress can be expanded by studying the functions of these genes. SfGPX was cloned from Spiraea fritschiana (S. fritschiana) with the highest low-temperature tolerance, to explore the molecular mechanisms of SfGPX in response to low-temperature stress and the physiological mechanisms involved in the regulation of SfGPX to adapt to low temperature, in two species of Spiraea. SfGPX, which was localized in the cytoplasm, was induced by low temperature. The low-temperature tolerance of Spiraea fritschiana was decreased via the interference of SfGPX, and the low-temperature tolerance of Spiraea japonica 'Gold Mound' (S. japonica 'Gold Mound') was elevated via the overexpression of SfGPX. Under low-temperature stress, the photosynthetic capacity of two species of Spiraea was affected by SfGPX; it was higher in the cold-tolerant plants and lower in the cold-intolerant plants. Under low-temperature stress, the transfer intensity of Ca2+ was affected by SfGPX. The transfer intensity of cold-tolerant plants with lower influx level of Ca2+ kinetics was weaker than that of cold-intolerant plants. Under low-temperature stress, the transfer velocity of Ca2+ was affected by SfGPX, and there were slower effluxes of Ca2+ from Ca2+ reservoir in cold-tolerant plants than in cold-intolerant plants. The above results indicate that the response of Spiraea to low temperature is regulated by SfGPX through affecting photosynthetic capacity as well as intensity and velocity of Ca2+ transfer in response to low temperature in Spiraea.


Assuntos
Spiraea , Temperatura Baixa , Regulação da Expressão Gênica de Plantas , Proteínas de Plantas/metabolismo , Spiraea/metabolismo , Temperatura
14.
Dev Cell ; 57(8): 947-958, 2022 04 25.
Artigo em Inglês | MEDLINE | ID: mdl-35417676

RESUMO

The dramatic temperature fluctuations spurred by climate change inhibit plant growth and threaten crop productivity. Unraveling how plants defend themselves against temperature-stress-induced cellular impairment is not only a crucial fundamental issue but is also of critical importance for agricultural sustainability and food security. Here, we review recent developments in elucidating the molecular mechanisms used by plants to sense and respond to cold and heat stress at multiple levels. We also describe the trade-off between plant growth and responses to high and low temperatures. Finally, we discuss possible strategies that could be used to engineer temperature-stress-tolerant, high-yielding crops.


Assuntos
Resposta ao Choque Térmico , Desenvolvimento Vegetal , Temperatura Baixa , Produtos Agrícolas , Resposta ao Choque Térmico/fisiologia , Estresse Fisiológico , Temperatura
15.
J Am Chem Soc ; 144(16): 7198-7207, 2022 Apr 27.
Artigo em Inglês | MEDLINE | ID: mdl-35427450

RESUMO

Although cold denaturation is a fundamental phenomenon common to all proteins, it can only be observed in a handful of cases where it occurs at temperatures above the freezing point of water. Understanding the mechanisms that determine cold denaturation and the rules that permit its observation is an important challenge. A way to approach them is to be able to induce cold denaturation in an otherwise stable protein by means of mutations. Here, we studied CyaY, a relatively stable bacterial protein with no detectable cold denaturation and a high melting temperature of 54 °C. We have characterized for years the yeast orthologue of CyaY, Yfh1, a protein that undergoes cold and heat denaturation at 5 and 35 °C, respectively. We demonstrate that, by transferring to CyaY the lessons learnt from Yfh1, we can induce cold denaturation by introducing a restricted number of carefully designed mutations aimed at destabilizing the overall fold and inducing electrostatic frustration. We used molecular dynamics simulations to rationalize our findings and demonstrate the individual effects observed experimentally with the various mutants. Our results constitute the first example of rationally designed cold denaturation and demonstrate the importance of electrostatic frustration on the mechanism of cold denaturation.


Assuntos
Temperatura Baixa , Proteínas , Temperatura Alta , Simulação de Dinâmica Molecular , Desnaturação Proteica , Termodinâmica
16.
Ecotoxicol Environ Saf ; 236: 113498, 2022 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-35421825

RESUMO

BACKGROUND: Exposure to non-optimum ambient temperature has been linked to increased risk of total cardiovascular disease (CVD) mortality; however, the adverse effects on mortality from specific types of CVD remain less understood. OBJECTIVES: To comprehensively investigate the association of ambient temperature with cause-specific CVD mortality, and to estimate and compare the corresponding mortality burden. METHODS: We conducted a time-stratified case-crossover study of 1000,014 CVD deaths in Jiangsu province, China during 2015-2019 using data from the China National Mortality Surveillance System. Residential daily 24-hour average temperature for each subject was extracted from a validated grid data at a spatial resolution of 0.0625° × 0.0625°. We fitted distributed lag non-linear models (DLNM) based on conditional logistic regression to quantitatively investigate the association of ambient temperature with total and cause-specific CVD mortality, which was used to further estimate mortality burden attributable to non-optimum ambient temperatures. RESULTS: With adjustment for relative humidity, we observed reverse J-shaped exposure-response associations of ambient temperature with total and cause-specific CVD mortality, with minimum mortality temperatures ranging from 19.5 °C to 23.0 °C. An estimated 20.3% of the total CVD deaths were attributable to non-optimum temperatures, while the attributable fraction (AF) of mortality from chronic rheumatic heart diseases, hypertensive diseases, ischemic heart diseases (IHD), pulmonary heart disease, stroke, and sequelae of stroke was 22.4%, 23.2%, 23.3%, 20.9%, 17.6% and 21.3%, respectively. For total and cause-specific CVDs, most deaths were attributable to moderate cold temperature. We observed significantly higher mortality burden from total and certain cause-specific CVDs in adults 80 years or older and those who were widowed. CONCLUSION: Exposure to ambient temperature was significantly associated with increased risk of cause-specific CVD mortality. The burden of CVD mortality attributable to non-optimum temperature was substantial especially in older and widowed adults, and significantly varied across specific types of CVD.


Assuntos
Doenças Cardiovasculares , Acidente Vascular Cerebral , Adulto , Idoso , China/epidemiologia , Temperatura Baixa , Estudos Cross-Over , Temperatura Alta , Humanos , Temperatura
17.
18.
J Plant Physiol ; 272: 153692, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35395488

RESUMO

Photoautotrophic organisms face extreme conditions in the Polar Regions including permanently low temperatures, freezing, salinity and low nutrient. Certain microalgae and cyanobacteria are able to withstand these conditions and adaptation mechanisms associated with photophysiology play an important part in overcoming challenges created by variation in irradiance under low temperatures.


Assuntos
Clima Frio , Cianobactérias , Aclimatação , Temperatura Baixa , Fotossíntese
19.
J Food Sci ; 87(5): 2072-2082, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35415844

RESUMO

This research aimed to investigate the oxidation and sorption isotherm properties of wheat germ and to determine monolayer moisture content (m0 ) in particular, because it has a limiting effect on lipid oxidation. This research comprised two stages. The first stage was the determination of sorption properties and m0 at three temperatures (15, 25, and 35°C). The second stage of the research was to investigate lipid oxidation properties of stored wheat germ at two different temperatures (4 and 25°C) and m0 for 28 days. As a result, it was determined that wheat germ has a Type II sorption isotherm, Halsey is the best fitting sorption equation, and the average m0 is 4.25 g moisture per 100 g dry matter. Furthermore, the free fatty acid and peroxide value of wheat germ oil increased with increasing storage moisture content and temperature, determined as 12.63% and 5.01 mEqO2 /kg on average, respectively. In conclusion, storage of impermeably packaged wheat germ at low temperature and m0 content is an applicable method for decreasing rancidity and prolonging shelf-life.


Assuntos
Grão Comestível , Triticum , Temperatura Baixa , Ácidos Graxos não Esterificados , Metabolismo dos Lipídeos
20.
Rev Bras Enferm ; 75(4): e20210620, 2022.
Artigo em Inglês, Português | MEDLINE | ID: mdl-35442311

RESUMO

OBJECTIVE: to analyze the accuracy of the defining characteristics of hypothermia in patients on hemodialysis. METHODS: a diagnostic accuracy study was assembled within a cross-sectional study with 124 patients from two dialysis centers. A latent class model was used for data analysis. RESULTS: the nursing diagnosis hypothermia was present in 13 (10.48%) study participants. The most prevalent defining characteristics were hypoxia (100%), decrease in blood glucose level (83.1%), hypertension (65.3%), piloerection (45.2%), and skin cool to touch (41.1%). The defining characteristics acrocyanosis (99.96%) and cyanotic nail beds (99.98%) had a high sensitivity. Acrocyanosis (91.8%), skin cool to touch (64.8%), and peripheral vasoconstriction (91.8%) had high specificity. CONCLUSION: specific and sensitive indicators of hypothermia work as good clinical indicators for confirming this diagnosis in patients on hemodialysis. The study findings can assist nurses in their clinical reasoning for a correct inference of hypothermia.


Assuntos
Hipotermia , Diagnóstico de Enfermagem , Temperatura Baixa , Estudos Transversais , Humanos , Hipotermia/complicações , Hipotermia/diagnóstico , Diálise Renal
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA