Development of a self-cloning system for Actinomadura verrucosospora and identification of polyketide synthase genes essential for production of the angucyclic antibiotic pradimicin.
Appl Environ Microbiol
; 65(6): 2703-9, 1999 Jun.
Article
in En
| MEDLINE
| ID: mdl-10347064
ABSTRACT
A self-cloning system for Actinomadura verrucosospora, a producer of the angucyclic antibiotic pradimicin A (PRM A), has been developed. The system is based on reproducible and reliable protoplasting and regeneration conditions for A. verrucosospora and a novel plasmid vector that consists of a replicon from a newly found Actinomadura plasmid and a selectable marker cloned from the Actinomadura strain. The system has an efficiency of more than 10(5) CFU/microgram of DNA. Using this system, we have cloned and identified the polyketide synthase (PKS) genes essential for PRM A biosynthesis from A. verrucosospora. Nucleotide sequence analysis of the 3.5-kb SalI-SphI fragment showed that ketosynthase subunits (open reading frame 1 [ORF1] and ORF2) of the essential PKS genes have strong similarities (59 to 89%) to those for angucyclic antibiotic biosynthesis.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Actinomycetales
/
Anthracyclines
/
Antibiotics, Antineoplastic
/
Multienzyme Complexes
/
Antifungal Agents
Type of study:
Diagnostic_studies
Language:
En
Journal:
Appl Environ Microbiol
Year:
1999
Document type:
Article