Construction and preliminary characterization of a library of "lethal" preterminal protein mutant adenoviruses.
J Virol
; 73(11): 9599-603, 1999 Nov.
Article
in En
| MEDLINE
| ID: mdl-10516069
ABSTRACT
Adenoviruses containing lethal in-frame insertion mutant alleles of the preterminal protein (pTP) gene were constructed with cell lines that express pTP. Thirty in-frame insertion mutant alleles, including 26 alleles previously characterized as lethal and 4 newly constructed mutant alleles, were introduced into the viral chromosome in place of the wild-type pTP gene. The viruses were tested for ability to form plaques at 37 degrees C in HeLa-pTP cells and at 32 degrees C and 39.5 degrees C in HeLa cells. Two of the newly constructed viruses exhibited temperature sensitivity for plaque formation, one virus did not form plaques in the absence of complementation, seven additional mutants exhibited a greater than 10-fold reduction in plaque formation in the absence of complementation, and another eight mutants exhibited stronger phenotypes than did previously characterized in-frame insertion mutants in the plaque assay. These mutant viruses offer promise for analysis of pTP functions.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phosphoproteins
/
Protein Precursors
/
Gene Library
/
Adenoviruses, Human
/
Genes, Lethal
Limits:
Humans
Language:
En
Journal:
J Virol
Year:
1999
Document type:
Article