Immunogenicity. I. Use of peptide libraries to identify epitopes that activate clonotypic CD4+ T cells and induce T cell responses to native peptide ligands.
J Immunol
; 163(12): 6424-34, 1999 Dec 15.
Article
in En
| MEDLINE
| ID: mdl-10586032
ABSTRACT
Recent studies have demonstrated the utility of synthetic combinatorial libraries for the rapid identification of peptide ligands that stimulate clonotypic populations of T cells. Here we screen a decapeptide combinatorial library arranged in a positional scanning format with two different clonotypic populations of CD4+ T cells to identify peptide epitopes that stimulate proliferative responses by these T cells in vitro. An extensive collection of mimic peptide sequences was synthesized and used to explore the fine specificity of TCR/peptide/MHC interactions. We also demonstrate that many of these deduced ligands are not only effective immunogens in vivo, but are capable of inducing T cell responses to the original native ligands used to generate the clones. These results have significant implications for considerations of T cell specificity and the design of peptide vaccines for infectious disease and cancer using clinically relevant T cell clones of unknown specificity.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Peptides
/
Lymphocyte Activation
/
CD4-Positive T-Lymphocytes
/
Epitopes, T-Lymphocyte
/
Peptide Library
Limits:
Animals
/
Female
/
Humans
Language:
En
Journal:
J Immunol
Year:
1999
Document type:
Article