Production and functional analysis of rat CD59 and chimeric CD59-Crry as active soluble proteins in Pichia pastoris.

Quigg, R J; He, C; Hack, B K; Alexander, J J; Morgan, B P

Quigg, R J; He, C; Hack, B K; Alexander, J J; Morgan, B P.

Affiliation

Quigg RJ; Department of Medicine, Section of Nephrology, The University of Chicago, Chicago, IL 60637, USA.

Immunology ; 99(1): 46-53, 2000 Jan.

Article in En | MEDLINE | ID: mdl-10651940

Subject(s)

CD59 Antigens/biosynthesis; Complement Inactivator Proteins; Pichia/metabolism; Receptors, Complement/biosynthesis; Recombinant Fusion Proteins/biosynthesis; Recombinant Proteins/biosynthesis; Animals; Antigens, Surface; CD59 Antigens/analysis; CD59 Antigens/immunology; Fermentation; Hemolysis/drug effects; Immunoblotting; Rats; Receptors, Cell Surface; Receptors, Complement/analysis; Receptors, Complement/immunology

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Pichia / Recombinant Fusion Proteins / Recombinant Proteins / Complement Inactivator Proteins / Receptors, Complement / CD59 Antigens Limits: Animals Language: En Journal: Immunology Year: 2000 Document type: Article

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