Triple helix assembly and processing of human collagen produced in transgenic tobacco plants.
FEBS Lett
; 469(1): 132-6, 2000 Mar 03.
Article
in En
| MEDLINE
| ID: mdl-10708770
ABSTRACT
The use of tobacco plants as a novel expression system for the production of human homotrimeric collagen I is presented in this report. Constructs were engineered from cDNA encoding the human proalpha1(I) chain to generate transgenic tobacco plants expressing collagen I. The recombinant proalpha1(I) chains were expressed as disulfide-bonded trimers and were shown to fold into a stable homotrimeric triple helix. Moreover, the recombinant procollagen was subsequently processed to collagen as it occurs in animals. Large amounts of recombinant collagen were purified from field grown plant material. The data suggest that plants are a valuable alternative for the recombinant production of collagen for various medical and scientific purposes.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plants, Toxic
/
Nicotiana
/
Procollagen
Limits:
Humans
Language:
En
Journal:
FEBS Lett
Year:
2000
Document type:
Article