AMP decreases the efficiency of skeletal-muscle mitochondria.
Biochem J
; 351 Pt 2: 307-11, 2000 Oct 15.
Article
in En
| MEDLINE
| ID: mdl-11023814
ABSTRACT
Mitochondrial proton leak in rat muscle is responsible for approx. 15% of the standard metabolic rate, so its modulation could be important in regulating metabolic efficiency. We report in the present paper that physiological concentrations of AMP (K(0.5)=80 microM) increase the resting respiration rate and double the proton conductance of rat skeletal-muscle mitochondria. This effect is specific for AMP. AMP also doubles proton conductance in skeletal-muscle mitochondria from an ectotherm (the frog Rana temporaria), suggesting that AMP activation is not primarily for thermogenesis. AMP activation in rat muscle mitochondria is unchanged when uncoupling protein-3 is doubled by starvation, indicating that this protein is not involved in the AMP effect. AMP activation is, however, abolished by inhibitors and substrates of the adenine nucleotide translocase (ANT), suggesting that this carrier (possibly the ANT1 isoform) mediates AMP activation. AMP activation of ANT could be important for physiological regulation of metabolic rate.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Atractyloside
/
Adenosine Monophosphate
/
Muscle, Skeletal
/
Mitochondria
Limits:
Animals
Language:
En
Journal:
Biochem J
Year:
2000
Document type:
Article