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Evidence that helix-loop-helix proteins collaborate with retinoblastoma tumor suppressor protein to regulate cortical neurogenesis.
Toma, J G; El-Bizri, H; Barnabe-Heider, F; Aloyz, R; Miller, F D.
Affiliation
  • Toma JG; Center for Neuronal Survival, Montreal Neurological Institute, Montreal, Canada H3A 2B4.
J Neurosci ; 20(20): 7648-56, 2000 Oct 15.
Article in En | MEDLINE | ID: mdl-11027225
ABSTRACT
The retinoblastoma tumor suppressor protein (pRb) family is essential for cortical progenitors to exit the cell cycle and survive. In this report, we test the hypothesis that pRb collaborates with basic helix-loop-helix (bHLH) transcription factors to regulate cortical neurogenesis, taking advantage of the naturally occurring dominant-inhibitory HLH protein Id2. Overexpression of Id2 in cortical progenitors completely inhibited the induction of neuron-specific genes and led to apoptosis, presumably as a consequence of conflicting differentiation signals. Both of these phenotypes were rescued by coexpression of a constitutively activated pRb mutant. In contrast, Id2 overexpression in postmitotic cortical neurons affected neither neuronal gene expression nor survival. Thus, pRb collaborates with HLHs to ensure the coordinate induction of terminal mitosis and neuronal gene expression as cortical progenitors become neurons.
Subject(s)

Full text: 1 Collection: 01-internacional Health context: 6_ODS3_enfermedades_notrasmisibles Database: MEDLINE Main subject: Repressor Proteins / Transcription Factors / Cerebral Cortex / Retinoblastoma Protein / Helix-Loop-Helix Motifs / Neurons Limits: Animals Language: En Journal: J Neurosci Year: 2000 Document type: Article

Full text: 1 Collection: 01-internacional Health context: 6_ODS3_enfermedades_notrasmisibles Database: MEDLINE Main subject: Repressor Proteins / Transcription Factors / Cerebral Cortex / Retinoblastoma Protein / Helix-Loop-Helix Motifs / Neurons Limits: Animals Language: En Journal: J Neurosci Year: 2000 Document type: Article