Structural basis for lipoxygenase specificity. Conversion of the human leukocyte 5-lipoxygenase to a 15-lipoxygenating enzyme species by site-directed mutagenesis.

Schwarz, K; Walther, M; Anton, M; Gerth, C; Feussner, I; Kuhn, H

Schwarz, K; Walther, M; Anton, M; Gerth, C; Feussner, I; Kuhn, H.

Affiliation

Schwarz K; Institute of Biochemistry, University Clinics Charité, Humboldt-University, Hessische Strasse 3-4, D-10115 Berlin, Germany.

J Biol Chem ; 276(1): 773-9, 2001 Jan 05.

Article in En | MEDLINE | ID: mdl-11027682

Subject(s)

Arachidonate 15-Lipoxygenase/chemistry; Arachidonate 15-Lipoxygenase/metabolism; Arachidonate 5-Lipoxygenase/chemistry; Arachidonate 5-Lipoxygenase/metabolism; Leukocytes/enzymology; Mutagenesis, Site-Directed/genetics; Amino Acid Sequence; Amino Acid Substitution/genetics; Arachidonate 15-Lipoxygenase/genetics; Arachidonate 5-Lipoxygenase/genetics; Binding Sites; Chromatography, High Pressure Liquid; Humans; Hydrogen-Ion Concentration; Hydroxyeicosatetraenoic Acids/analysis; Hydroxyeicosatetraenoic Acids/chemistry; Hydroxyeicosatetraenoic Acids/metabolism; Isomerism; Molecular Sequence Data; Point Mutation; Recombinant Proteins/chemistry; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Sequence Alignment; Substrate Specificity

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Collection: 01-internacional Database: MEDLINE Main subject: Arachidonate 5-Lipoxygenase / Arachidonate 15-Lipoxygenase / Mutagenesis, Site-Directed / Leukocytes Limits: Humans Language: En Journal: J Biol Chem Year: 2001 Document type: Article

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