Discrepancies between apolipoprotein E phenotyping and genotyping in the elderly.
Clin Chem Lab Med
; 39(5): 405-13, 2001 May.
Article
in En
| MEDLINE
| ID: mdl-11434390
ABSTRACT
We estimated the frequencies of phenotype (isoelectric focusing; IEF) vs. genotype (PCR/Hhal) discordance in a sample of an aged population (> 65 years). Both phenotype and genotype techniques have been used in the study of apolipoprotein E (apoE) polymorphism in 125 elderly subjects. The discordance between phenotype and genotype was unresolved in 11 (8.8%) of the 125 unrelated subjects studied. We observed a significant association between the presence of the E4 allele and both Alzheimer's disease (chi2 = 13, p < 0.001) and increased cholesterol concentration (Mann Whitney, p < 0.03). These relationships were not affected by the techniques used. Our results indicate that transcriptional modulation and post-transductional modifications in normal ageing and in aged-related diseases may explain in part discrepancies between gene analysis and protein characterisation.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Apolipoproteins E
Limits:
Aged
/
Aged80
/
Female
/
Humans
/
Male
Language:
En
Journal:
Clin Chem Lab Med
Year:
2001
Document type:
Article