Chicken interleukin-6. cDNA structure and biological properties.
Eur J Biochem
; 268(15): 4200-6, 2001 Aug.
Article
in En
| MEDLINE
| ID: mdl-11488913
ABSTRACT
Suppression subtractive hybridization technology was used to identify differentially expressed genes in spleens of chickens that had been treated with the synthetic immune modifier S-28463. One induced chicken gene encoded a protein with about 35% sequence identity to human interleukin-6 (IL-6). It consists of 241 amino acids including a putative N-terminal signal peptide of 47 residues. Bacterially expressed chicken IL-6 (ChIL-6) carrying a histidine tag in place of the signal peptide was biologically active it induced proliferation of the IL-6-dependent murine hybridoma cell line 7TD1. The concentration of ChIL-6 required for half-maximal proliferative response was approximately 60 pg.mL-1. When injected intravenously into adult chickens, purified recombinant ChIL-6 induced an increase in serum corticosterone levels. Supernatants of chicken LMH and monkey COS-7 cells transiently transfected with a ChIL-6 expression construct induced proliferation of 7TD1 cells, demonstrating that recombinant ChIL-6 from eukaryotic cells is also active.
Search on Google
Collection:
01-internacional
Health context:
3_ND
Database:
MEDLINE
Main subject:
Interleukin-6
/
DNA, Complementary
Type of study:
Prognostic_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Eur J Biochem
Year:
2001
Document type:
Article