RNA-RNA noncovalent interactions investigated by microspray ionization mass spectrometry.
Rapid Commun Mass Spectrom
; 15(17): 1539-47, 2001.
Article
in En
| MEDLINE
| ID: mdl-11544590
ABSTRACT
Electrospray ionization mass spectrometry is playing an increasing role in the study of noncovalent interactions involving biomolecules. RNA-RNA complexes are important in many areas of biology, including RNA catalysis, RNA splicing, ribosome function, and gene regulation. Here, microelectrospray mass spectrometry (microESI-MS) is used to study noncovalent base-pairing interactions between RNA oligonucleotides, an area not previously explored by this technique. Using a set of complementary RNA oligonucleotides, we demonstrate the formation of the expected double-helical RNA complexes composed of three distinct oligonucleotides. The ability to study specific RNA noncovalent interactions by microESI-MS has the potential to provide a unique method by which to analyze and assign precise molecular masses to RNA-RNA complexes.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oligonucleotides
/
RNA
/
Spectrometry, Mass, Electrospray Ionization
Language:
En
Journal:
Rapid Commun Mass Spectrom
Year:
2001
Document type:
Article