DNA analysis for the Dombrock polymorphism.
Transfusion
; 41(9): 1143-6, 2001 Sep.
Article
in En
| MEDLINE
| ID: mdl-11552072
ABSTRACT
BACKGROUND:
RBC typing for Do(a) and Do(b) is notoriously difficult, and inaccurate typing can predispose patients to hemolytic transfusion reactions. The DO1/DO2 polymorphism is associated with three nucleotide changes 378 C>T, 624 T>C and 793 A>G. While the 378 C>T- and 624 T>C-containing codons are silent mutations, the 793 A>G polymorphism in codon 265 encodes asparagine for Do(a) and aspartic acid for Do(b). STUDY DESIGN ANDMETHODS:
Described here are two PCR-RFLP assays, one using the Mnl I site associated with 624C (DO2) and the other altering two nucleotides within the sense primer, which allows recognition of 793G by the Eam 1105 I.RESULTS:
The assays have been performed on over 100 samples for which the RBC typing of one or both antigens was known. Eight samples had been historically mistyped by hemagglutination.CONCLUSION:
This RFLP assay provides a practical method for typing donor blood for Dombrock alleles.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Polymorphism, Genetic
/
Blood Group Antigens
/
DNA
Limits:
Humans
Language:
En
Journal:
Transfusion
Year:
2001
Document type:
Article