Increased production of active human beta(2)-adrenergic/G(alphas) fusion receptor in Sf-9 cells using nutrient limiting conditions.

Carpentier, E; Lebesgue, D; Kamen, A A; Hogue, M; Bouvier, M; Durocher, Y

Carpentier, E; Lebesgue, D; Kamen, A A; Hogue, M; Bouvier, M; Durocher, Y.

Affiliation

Carpentier E; Bioprocess sector, Biotechnology Research Institute, 6100 Royalmount avenue, Montreal, Quebec, H4P 2R2, Canada

Protein Expr Purif ; 23(1): 66-74, 2001 Oct.

Article in En | MEDLINE | ID: mdl-11570847

Subject(s)

Heterotrimeric GTP-Binding Proteins/biosynthesis; Receptors, Adrenergic, beta-2/biosynthesis; Recombinant Fusion Proteins/biosynthesis; Animals; Baculoviridae/genetics; Baculoviridae/growth & development; Cell Culture Techniques/methods; Cell Line; Culture Media; Heterotrimeric GTP-Binding Proteins/genetics; Humans; Immunoassay; Insecta; Protein Binding; Protein Subunits; Receptors, Adrenergic, beta-2/genetics; Receptors, Adrenergic, beta-2/immunology; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/immunology; Transduction, Genetic

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PubMed Links

Collection: 01-internacional Database: MEDLINE Main subject: Recombinant Fusion Proteins / Receptors, Adrenergic, beta-2 / Heterotrimeric GTP-Binding Proteins Limits: Animals / Humans Language: En Journal: Protein Expr Purif Year: 2001 Document type: Article

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