Purification of engineered human pancreatic ribonuclease.

Ribó, M; Benito, A; Canals, A; Nogués, M V; Cuchillo, C M; Vilanova, M

Ribó, M; Benito, A; Canals, A; Nogués, M V; Cuchillo, C M; Vilanova, M.

Affiliation

Ribó M; Laboratori d'Enginyeria de Proteïnes, Departament de Biologia, Universitat de Girona, E-17071 Girona, Spain.

Methods Enzymol ; 341: 221-34, 2001.

Article in En | MEDLINE | ID: mdl-11582779

Subject(s)

Ribonuclease, Pancreatic/isolation & purification; Chromatography, Ion Exchange/methods; Circular Dichroism; Humans; Kinetics; Oxidation-Reduction; Protein Conformation; Protein Denaturation; Protein Engineering; Protein Folding; Recombinant Proteins/chemistry; Recombinant Proteins/isolation & purification; Recombinant Proteins/metabolism; Ribonuclease, Pancreatic/chemistry; Ribonuclease, Pancreatic/genetics; Substrate Specificity

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PubMed Links

Collection: 01-internacional Database: MEDLINE Main subject: Ribonuclease, Pancreatic Limits: Humans Language: En Journal: Methods Enzymol Year: 2001 Document type: Article

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