The protein kinase Pelle mediates feedback regulation in the Drosophila Toll signaling pathway.
Development
; 128(23): 4729-36, 2001 Dec.
Article
in En
| MEDLINE
| ID: mdl-11731453
ABSTRACT
Dorsoventral polarity in the Drosophila embryo is established through a signal transduction cascade triggered in ventral and ventrolateral regions. Activation of a transmembrane receptor, Toll, leads to localized recruitment of the adaptor protein Tube and protein kinase Pelle. Signaling through these components directs degradation of the IkappaB-like inhibitor Cactus and nuclear translocation of the Rel protein Dorsal. Here we show through confocal immunofluorescence microscopy that Pelle functions to downregulate the signal-dependent relocalization of Tube. Inactivation of the Pelle kinase domain, or elimination of the Tube-Pelle interaction, dramatically increases Tube recruitment to the ventral plasma membrane in regions of active signaling. We also characterize a large collection of pelle alleles, identifying the molecular lesions in these alleles and their effects on Pelle autophosphorylation, Tube phosphorylation and Tube relocalization. Our results point to a mechanism operating to modulate the domain or duration of signaling downstream from Tube and Pelle.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Membrane Glycoproteins
/
Protein Serine-Threonine Kinases
/
Receptors, Cell Surface
/
Insect Proteins
/
Drosophila Proteins
/
Drosophila melanogaster
Limits:
Animals
Language:
En
Journal:
Development
Year:
2001
Document type:
Article