Single-tube genotyping of MBL-2 polymorphisms using melting temperature analysis.
Clin Exp Med
; 2(2): 105-8, 2002 Jul.
Article
in En
| MEDLINE
| ID: mdl-12141527
ABSTRACT
Recently several authors correlated MBL-2 gene polymorphisms with different pathologies and there is a growing interest for MBL-2 genotyping in a large number of individuals. We have developed a single-tube, rapid, economic, and fully automated melting temperature analysis screening method, based on ABI 7700 Sequence Detection System technology and SYBR Green I chemistry, for the detection of three polymorphisms (exon 1, codons 52, 54, 57) in the MBL-2 gene. We also developed an electronic sheet for the automatic calling of different genotypes, based on the analysis of the first derivative of ABI 7700 raw data.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Polymorphism, Genetic
/
Carrier Proteins
/
Genetic Techniques
Type of study:
Evaluation_studies
Limits:
Humans
Language:
En
Journal:
Clin Exp Med
Year:
2002
Document type:
Article