Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis.

Paulsen, I T; Banerjei, L; Myers, G S A; Nelson, K E; Seshadri, R; Read, T D; Fouts, D E; Eisen, J A; Gill, S R; Heidelberg, J F; Tettelin, H; Dodson, R J; Umayam, L; Brinkac, L; Beanan, M; Daugherty, S; DeBoy, R T; Durkin, S; Kolonay, J; Madupu, R; Nelson, W; Vamathevan, J; Tran, B; Upton, J; Hansen, T; Shetty, J; Khouri, H; Utterback, T; Radune, D; Ketchum, K A; Dougherty, B A; Fraser, C M

Paulsen, I T; Banerjei, L; Myers, G S A; Nelson, K E; Seshadri, R; Read, T D; Fouts, D E; Eisen, J A; Gill, S R; Heidelberg, J F; Tettelin, H; Dodson, R J; Umayam, L; Brinkac, L; Beanan, M; Daugherty, S; DeBoy, R T; Durkin, S; Kolonay, J; Madupu, R; Nelson, W; Vamathevan, J; Tran, B; Upton, J; Hansen, T; Shetty, J; Khouri, H; Utterback, T; Radune, D; Ketchum, K A; Dougherty, B A; Fraser, C M.

Affiliation

Paulsen IT; Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA. ipaulsen@tigr.org

Science ; 299(5615): 2071-4, 2003 Mar 28.

Article in En | MEDLINE | ID: mdl-12663927

ABSTRACT

ABSTRACT

The complete genome sequence of Enterococcus faecalis V583, a vancomycin-resistant clinical isolate, revealed that more than a quarter of the genome consists of probable mobile or foreign DNA. One of the predicted mobile elements is a previously unknown vanB vancomycin-resistance conjugative transposon. Three plasmids were identified, including two pheromone-sensing conjugative plasmids, one encoding a previously undescribed pheromone inhibitor. The apparent propensity for the incorporation of mobile elements probably contributed to the rapid acquisition and dissemination of drug resistance in the enterococci.

Subject(s)

Biological Evolution; Enterococcus faecalis/genetics; Genome, Bacterial; Interspersed Repetitive Sequences; Sequence Analysis, DNA; Vancomycin Resistance/genetics; Adhesins, Bacterial/genetics; Bacterial Adhesion; Bacterial Proteins/genetics; Carrier Proteins/genetics; Carrier Proteins/metabolism; Chromosomes, Bacterial/genetics; Conjugation, Genetic; Conserved Sequence; DNA Transposable Elements; Digestive System/microbiology; Drug Resistance, Multiple, Bacterial; Enterococcus faecalis/drug effects; Enterococcus faecalis/pathogenicity; Enterococcus faecalis/physiology; Gene Transfer, Horizontal; Gram-Positive Bacterial Infections/microbiology; Humans; Lysogeny; Open Reading Frames; Oxidative Stress; Plasmids; Synteny; Virulence/genetics; Virulence Factors/genetics

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Collection: 01-internacional Database: MEDLINE Main subject: Genome, Bacterial / Enterococcus faecalis / Sequence Analysis, DNA / Interspersed Repetitive Sequences / Vancomycin Resistance / Biological Evolution Type of study: Prognostic_studies Limits: Humans Language: En Journal: Science Year: 2003 Document type: Article

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