[Preparation and utilization of BrdU antiserum. II. Detections of BrdU labelling chromosome and DNA using BrdU antibody technique].

ABSTRACT

Utilizing highly specific BrdU antiserum, via the second antibody conjugated with enzyme and DAB-H2O2 or AEC-H2O2 colorific methods, the BrdU marked regions of CHO and human chromosomes were detected clearly. The BrdU-DNA prints (pg) on nitrocellulose membranes were also showed with similar methods.