Creation of a productive, highly enantioselective nitrilase through gene site saturation mutagenesis (GSSM).

DeSantis, Grace; Wong, Kelvin; Farwell, Bob; Chatman, Kelly; Zhu, Zoulin; Tomlinson, Geoff; Huang, Hongjun; Tan, Xuqiu; Bibbs, Lisa; Chen, Pei; Kretz, Keith; Burk, Mark J

DeSantis, Grace; Wong, Kelvin; Farwell, Bob; Chatman, Kelly; Zhu, Zoulin; Tomlinson, Geoff; Huang, Hongjun; Tan, Xuqiu; Bibbs, Lisa; Chen, Pei; Kretz, Keith; Burk, Mark J.

Affiliation

DeSantis G; Diversa Corporation, 4955 Directors Place, San Diego, California 92121, USA.

J Am Chem Soc ; 125(38): 11476-7, 2003 Sep 24.

Article in En | MEDLINE | ID: mdl-13129332

Subject(s)

Aminohydrolases/chemistry; Aminohydrolases/genetics; Amino Acid Substitution; Aminohydrolases/metabolism; Hydroxybutyrates/chemical synthesis; Mutagenesis, Site-Directed; Stereoisomerism

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Collection: 01-internacional Database: MEDLINE Main subject: Aminohydrolases Language: En Journal: J Am Chem Soc Year: 2003 Document type: Article

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PubMed Links

Collection: 01-internacional Database: MEDLINE Main subject: Aminohydrolases Language: En Journal: J Am Chem Soc Year: 2003 Document type: Article