Protein separation using membrane-encapsulated soluble ligand conjugates.
Enzyme Microb Technol
; 12(5): 349-54, 1990 May.
Article
in En
| MEDLINE
| ID: mdl-1366487
ABSTRACT
A new approach for isolating and recovering biological macromolecules using membrane-encapsulated soluble ligand conjugates was investigated. Membrane-encapsulated solid adsorbents have been successfully developed and employed in our laboratory to isolate and purify proteins and enzymes directly from culture broths. This new concept also makes it possible to use soluble ligand conjugates instead of solid adsorbents inside membrane capsules. In this work, model membrane-encapsulated soluble and insoluble ligands comprising Blue Dextran and Blue Sepharose entrapped within calcium alginate membranes were studied to compare adsorption characteristics such as capacities and rates. Experimental results suggest that membrane-encapsulated soluble ligands may be expected to result in higher overall adsorption capacity compared to membrane-encapsulated solid adsorbents with comparable adsorption rates.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Proteins
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Enzyme Microb Technol
Year:
1990
Document type:
Article