Increase in synthesis of human monoclonal antibodies by transfected Sp2/0 myeloma mouse cell line under conditions of microgravity.
Biotechnol Lett
; 25(15): 1271-4, 2003 Aug.
Article
in En
| MEDLINE
| ID: mdl-14514080
ABSTRACT
Microgravity can influence cell growth and function. A transfected Sp2/0 myeloma cell line P3A2 producing a human IgG1 anti-TNFa monoclonal antibody was cultivated in static culture, spinner flasks and simulated microgravity using a rotating wall vessel bioreactor. Microgravity significantly decreased cell growth (from 1.7 x 10(6) to 7.9 x 10(5) cells/ml), but facilitated the synthesis of antibodies, (1.8, 1.3 and 0.5 microg of anti-TNFalpha hmAb per 10(6) viable cells for cells cultivated under microgravity, in spinner flasks and static cultures, respectively). The results suggest that microgravity could be applied to improve the specific productivity of cell lines producing potentially important therapeutic proteins.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Weightlessness
/
Bioreactors
/
Culture Techniques
/
Antibodies, Monoclonal
/
Multiple Myeloma
Type of study:
Evaluation_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Biotechnol Lett
Year:
2003
Document type:
Article