Mitochondrial matrix reactive oxygen species production is very sensitive to mild uncoupling.
Biochem Soc Trans
; 31(Pt 6): 1300-1, 2003 Dec.
Article
in En
| MEDLINE
| ID: mdl-14641047
ABSTRACT
Mitochondria produce ROS (reactive oxygen species) as a by-product of aerobic respiration. Several studies in mammals and birds suggest that the most physiologically relevant ROS production is from complex I following reverse electron flow, and is highly sensitive to membrane potential. A study of Drosophila mitochondria respiring glycerol 3-phosphate revealed that membrane potential-sensitive ROS production from complex I following reverse electron flow was on the matrix side of the inner membrane. A 10 mV decrease in membrane potential was enough to abolish around 70% of the ROS produced by complex I under these conditions. Another important ROS generator in this model, glycerol-3-phosphate dehydrogenase, produced ROS mostly to the cytosolic side; this ROS production was totally insensitive to a small decrease in membrane potential (10 mV). Thus mild uncoupling may be particularly significant for ROS production from complex I on the matrix side of the mitochondrial inner membrane.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Reactive Oxygen Species
/
Mitochondria
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
En
Journal:
Biochem Soc Trans
Year:
2003
Document type:
Article