The remodelled tracheal basement membrane zone of infant rhesus monkeys after 6 months of recovery.
Clin Exp Allergy
; 34(7): 1131-6, 2004 Jul.
Article
in En
| MEDLINE
| ID: mdl-15248862
ABSTRACT
BACKGROUND:
In previous studies, we showed that repeated exposure to (1) house dust mite allergen (HDMA) (Dermatophagoides farinae) caused thickening of the basement membrane zone (BMZ) and (2) HDMA+ozone (O3) caused depletion of BMZ perlecan and atypical development of BMZ collagen (irregular thin areas<2.0 microm in width).OBJECTIVE:
The purpose of this study was to determine if these remodelling changes were reversible after 6 months of recovery.METHODS:
Rhesus monkeys were exposed to a regimen of HDMA and or O3 or filtered air (FA) for 6 months. After the exposure protocol was completed FA and O3 groups were allowed to recover in FA for 6 months. The HDMA and HDMA+O3 exposure groups recovered in a modified environment. They were re-exposed to HDMA aerosol for 2 h at monthly intervals during recovery in order to maintain sensitization for pulmonary function testing. To detect structural changes in the BMZ, collagen I and perlecan immunoreactivity were measured and compared to data from the previous papers.RESULTS:
The remodelled HDMA group had a significantly thicker BMZ and after 6 months of recovery the width had not regressed. In the remodelled BMZ of the HDMA+O3 group, perlecan had returned to the BMZ after 6 months of the recovery protocol, and the thin, irregular, collagen BMZ had been resolved.CONCLUSION:
In summary, this study has shown that (1) The width of the remodelled HDMA BMZ did not regress during a recovery protocol that included a sensitizing dose of HDMA. (2) The atypical collagen BMZ in the HDMA+O3 BMZ was resolved in the absence of O3. (3) Depletion of perlecan from the BMZ by O3 was reversed by recovery in the absence of O3.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Trachea
/
Basement Membrane
/
Dermatophagoides farinae
/
Antigens, Dermatophagoides
/
Hypersensitivity
Limits:
Animals
Language:
En
Journal:
Clin Exp Allergy
Year:
2004
Document type:
Article