[The influence of circadian gene mPeriod2 on Lewis cancer cell].

OBJECTIVE: To investigate the role of circadian gene mPeriod2 (mPer2) on tumor proliferation and apoptosis. METHOD: The eukaryotic mPer2 expression vector (pcDNA 3.1-mPer2) based on pcDNA 3.1 was transfected into the cultured Lewis tumor cell by liposome method in vivo. The expression of mPer2 in transfected Lewis tumor cell was detected by immunohistochemistry and flowcytometry. The stable transfected cell line was screened by G418 and its proliferation and apoptosis was examined by flowcytometry. RESULT: Expression of PERIOD2 protein in pcDNA 3.1-mPer2 transfected Lewis tumor cell was proved by immunohistochemistry and flowcytometry. Flowcytometry result of mPer2 expression Lewis tumor cell showed less proliferation and high rate of apoptosis. CONCLUSION: mPer2 expression can suppress the proliferation of tumor cell and increase the apoptosis of it.