An immunogold evaluation of cortical actin reorganization in pubertal Sertoli cells in vitro after PMA treatment.

Panagopoulou, Evridiki; Kouloukoussa, Mirsini; Kittas, Christos; Marinos, Evangelos

Panagopoulou, Evridiki; Kouloukoussa, Mirsini; Kittas, Christos; Marinos, Evangelos.

Affiliation

Panagopoulou E; University of Athens, Medical School, Department of Histology and Embryology, Mikras Asias 75, Goudi, Greece.

Cell Biol Int ; 29(11): 914-9, 2005 Nov.

Article in En | MEDLINE | ID: mdl-16198130

ABSTRACT

ABSTRACT

The aim of the present study was to investigate stress fibers and cortical actin reorganization in pubertal Sertoli cells in vitro after PMA treatment. Actin was studied by means of immunogold labeling, using the 'Progressive Lowering of Temperature' technique (PLT). Actin rearrangement was evaluated by a quantitative analysis of the gold label distribution. Eight hours after addition of 10(-7) M PMA, rearrangement of cortical actin was minimal, but stress fiber perturbation was significant as shown by immunogold labeling distribution measurements. PMA-mediated F-actin reorganization and redistribution in non-neoplastic cells is discussed, since these phenomena have been closely linked with cell transformation.

Subject(s)

Actins/chemistry; Immunohistochemistry/methods; Sertoli Cells/cytology; Tetradecanoylphorbol Acetate/pharmacology; Actin Cytoskeleton/drug effects; Animals; Carcinogens/pharmacology; Cells, Cultured; Cytoskeleton/metabolism; Male; Microscopy, Electron; Microscopy, Immunoelectron; Rats; Rats, Wistar; Sertoli Cells/metabolism; Temperature; Time Factors

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Collection: 01-internacional Database: MEDLINE Main subject: Sertoli Cells / Tetradecanoylphorbol Acetate / Immunohistochemistry / Actins Limits: Animals Language: En Journal: Cell Biol Int Year: 2005 Document type: Article

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