[Quantification of caffeine from energy drinks with mass spectrometry method (LC/MS)]. / Determinarea cafeinei din bauturi energizante prin LC/MS.
Rev Med Chir Soc Med Nat Iasi
; 109(3): 664-7, 2005.
Article
in Ro
| MEDLINE
| ID: mdl-16607769
ABSTRACT
A high performance liquid chromatography coupled with mass spectrometry method (LC/MS) for quantification of caffeine from energy drinks was elaborated. It was utilised a Zorbax SB-C18, 100 mm x 3.0 mm i.d., 3.5 microm column with a mobile phase containing methanol/ solution 0.2% formic acid in water. Detection and quantification of caffeine was based on monitoring the protonated molecular ion abundance (m/z 194.9). The quantification was made using the external standard method. The calibration curve were made on range 0.26-26 microg/ml for caffeine with a correlation coefficient greater than 0.995. There were analysed 12 energy drinks with a caffeine content between 13 and 34 mg/100 ml.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Beverages
/
Caffeine
/
Chromatography, High Pressure Liquid
/
Spectrometry, Mass, Electrospray Ionization
/
Central Nervous System Stimulants
Limits:
Humans
Language:
Ro
Journal:
Rev Med Chir Soc Med Nat Iasi
Year:
2005
Document type:
Article