Washout of heme-containing proteins dramatically improves tetrazolium-based infarct staining.

Pitts, Kelly R; Stiko, Ann; Buetow, Bernard; Lott, Fred; Guo, Ping; Virca, Duke; Toombs, Christopher F

Pitts, Kelly R; Stiko, Ann; Buetow, Bernard; Lott, Fred; Guo, Ping; Virca, Duke; Toombs, Christopher F.

Affiliation

Pitts KR; Inflammation Research, Amgen Inc., Seattle, WA 98119, USA. kelly.pitts@myogen.com

J Pharmacol Toxicol Methods ; 55(2): 201-8, 2007.

Article in En | MEDLINE | ID: mdl-16931060

Subject(s)

Artifacts; Hemeproteins/metabolism; Myocardial Infarction/pathology; Myocardial Reperfusion Injury/pathology; Staining and Labeling/methods; Animals; Chromatography, High Pressure Liquid; Coloring Agents/chemistry; Electrophoresis, Polyacrylamide Gel; Hemeproteins/chemistry; Hemorrhage/etiology; Hemorrhage/metabolism; Hemorrhage/pathology; Myocardial Infarction/complications; Myocardial Infarction/metabolism; Myocardial Reperfusion Injury/complications; Myocardial Reperfusion Injury/metabolism; Myocardium/chemistry; Myocardium/metabolism; Myocardium/pathology; Rats; Rats, Sprague-Dawley; Sequence Analysis, Protein; Tetrazolium Salts/chemistry

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Collection: 01-internacional Health context: 6_ODS3_enfermedades_notrasmisibles Database: MEDLINE Main subject: Staining and Labeling / Myocardial Reperfusion Injury / Artifacts / Hemeproteins / Myocardial Infarction Type of study: Etiology_studies Limits: Animals Language: En Journal: J Pharmacol Toxicol Methods Year: 2007 Document type: Article

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