Animal models for disease: knockout, knock-in, and conditional mutant mice.
Methods Mol Med
; 129: 41-67, 2006.
Article
in En
| MEDLINE
| ID: mdl-17085804
ABSTRACT
Diseases with a genetic basis can be modeled with knockout, knock-in, and conditional mutant gene-targeted mice. In the following, we provide detailed protocols for gene targeting. Gene targeting of embryonic stem cells can be accomplished by laboratories equipped for tissue culture. Alternatively, many gene-targeting services divide the work of targeting with a customer lab. In this collaborative situation, knowledge of the entire process helps ensure a successful outcome. The construction of chimeras for germ-line transmission is not described here, because this procedure is beyond the means of most laboratories, typically is provided by transgenic core facilities, and is best learned through hands-on demonstration.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Gene Targeting
/
Disease Models, Animal
/
Mice
Limits:
Animals
Language:
En
Journal:
Methods Mol Med
Year:
2006
Document type:
Article