Identification of the dual specificity and the functional domains of the cardiac-specific protein kinase TNNI3K.
Gen Physiol Biophys
; 26(2): 104-9, 2007 Jun.
Article
in En
| MEDLINE
| ID: mdl-17660584
ABSTRACT
Molecular cloning of cardiac troponin I-interacting kinase (TNNI3K), a novel cardiac-specific protein kinase containing seven N-terminal ankyrin (ANK) repeats followed by a protein kinase domain and a C-terminal Ser-rich domain, has previously been reported. In the present study, we show that the C-terminal functional region of TNNI3K negatively regulates the kinase activity, and the N-terminal ANK domain is necessary for autophosphorylation. An in vitro kinase assay shows that TNNI3K exhibits dual-specific kinase activity and forms dimers or oligomers that may be necessary for its activation.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Protein-Tyrosine Kinases
/
Protein Serine-Threonine Kinases
/
MAP Kinase Kinase Kinases
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
En
Journal:
Gen Physiol Biophys
Year:
2007
Document type:
Article