Production of the endocannabinoids anandamide and 2-arachidonoylglycerol by endothelial progenitor cells.
FEBS Lett
; 581(25): 4927-31, 2007 Oct 16.
Article
in En
| MEDLINE
| ID: mdl-17904123
ABSTRACT
Recent studies have highlighted the importance of paracrine growth factors as mediators of pro-angiogenic effects by endothelial progenitor cells (EPCs), but little is known about the release of lipid-based factors like endocannabinoids by EPCs. In the current study, the release of the endocannabinoids anandamide and 2-arachidonoylglycerol by distinct human EPC sub-types was measured using HPLC/tandem mass-spectrometry. Anandamide release was highest by adult blood colony-forming EPCs at baseline and they also demonstrated increased 2-arachidonoylglycerol release with TNF-alpha stimulation. Treatment of mature endothelial cells with endocannabinoids significantly reduced the induction of the pro-inflammatory adhesion molecule CD106 (VCAM-1) by TNF-alpha.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Stem Cells
/
Endothelium, Vascular
/
Arachidonic Acids
/
Endocannabinoids
/
Cannabinoid Receptor Modulators
/
Glycerides
Limits:
Humans
Language:
En
Journal:
FEBS Lett
Year:
2007
Document type:
Article