Chimerism analysis following nonmyeloablative stem cell transplantation using a new cell subset separation method: Robosep.
Biomed Mater Eng
; 18(1 Suppl): S19-26, 2008.
Article
in En
| MEDLINE
| ID: mdl-18334720
ABSTRACT
Chimerism analysis has become an important tool to manage patients in the peri-transplant period of allogenic stem cell transplantation. During this period, cells of donor and host origin can coexist and increasing proportion of cells of host origin is considered as a recurrence of the underlying disease. We currently performed chimerism analysis on separate peripheral blood cell subsets, lymphocytes and granulocytes. To improve our isolation method, a new automated device from Stem Cell Technology Roboseptrade mark was tested and compared to our manual separation technique. The results obtained on T cell purification showed an improvement of the purity (98.42% with Robosep vs. 92.42% with the manual technique Rosettesep) and of the recovery (63.43% with Robosep and 38% with Rosettesep). The results were significantly improved on patient samples with less than 10% CD3 positive cells (purity 90% vs. 44.44%; recovery 73.79% vs. 43.98%). Granulocytes separation was based on CD15 expression. The results showed an improvement of the purity with Robosep (96.90% vs. 86.20% with the manual technique Polymorphprep) but the recovery was impaired (35.2% vs. 52.30%). Using a myeloid (CD66/CD33) cocktail, recovery was improved with the Robosep device (64.04% with the myeloid cocktail vs. 22.4% with the CD15 cocktail). Our data demonstrated that Robosep allowed a performant cell purification in the early period post-transplantation even for populations representing less than 10% of the peripheral blood cells.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Blood Cell Count
/
Robotics
/
T-Lymphocytes
/
Cell Separation
/
Mesenchymal Stem Cell Transplantation
/
Mesenchymal Stem Cells
/
Granulocytes
Type of study:
Diagnostic_studies
/
Evaluation_studies
Limits:
Humans
/
Male
Language:
En
Journal:
Biomed Mater Eng
Year:
2008
Document type:
Article