Your browser doesn't support javascript.
loading
Chimerism analysis following nonmyeloablative stem cell transplantation using a new cell subset separation method: Robosep.
Decot, V; Latger-Cannard, V; Lecompte, T; Clément, L; Salmon, A; Bordigoni, P; Stoltz, J-F; Bensoussan, D.
Affiliation
  • Decot V; Cell Therapy Unit and Tissue Bank, CHU Nancy Brabois, Vandoeuvre-Les-Nancy, France.
Biomed Mater Eng ; 18(1 Suppl): S19-26, 2008.
Article in En | MEDLINE | ID: mdl-18334720
ABSTRACT
Chimerism analysis has become an important tool to manage patients in the peri-transplant period of allogenic stem cell transplantation. During this period, cells of donor and host origin can coexist and increasing proportion of cells of host origin is considered as a recurrence of the underlying disease. We currently performed chimerism analysis on separate peripheral blood cell subsets, lymphocytes and granulocytes. To improve our isolation method, a new automated device from Stem Cell Technology Roboseptrade mark was tested and compared to our manual separation technique. The results obtained on T cell purification showed an improvement of the purity (98.42% with Robosep vs. 92.42% with the manual technique Rosettesep) and of the recovery (63.43% with Robosep and 38% with Rosettesep). The results were significantly improved on patient samples with less than 10% CD3 positive cells (purity 90% vs. 44.44%; recovery 73.79% vs. 43.98%). Granulocytes separation was based on CD15 expression. The results showed an improvement of the purity with Robosep (96.90% vs. 86.20% with the manual technique Polymorphprep) but the recovery was impaired (35.2% vs. 52.30%). Using a myeloid (CD66/CD33) cocktail, recovery was improved with the Robosep device (64.04% with the myeloid cocktail vs. 22.4% with the CD15 cocktail). Our data demonstrated that Robosep allowed a performant cell purification in the early period post-transplantation even for populations representing less than 10% of the peripheral blood cells.
Subject(s)
Search on Google
Collection: 01-internacional Database: MEDLINE Main subject: Blood Cell Count / Robotics / T-Lymphocytes / Cell Separation / Mesenchymal Stem Cell Transplantation / Mesenchymal Stem Cells / Granulocytes Type of study: Diagnostic_studies / Evaluation_studies Limits: Humans / Male Language: En Journal: Biomed Mater Eng Year: 2008 Document type: Article
Search on Google
Collection: 01-internacional Database: MEDLINE Main subject: Blood Cell Count / Robotics / T-Lymphocytes / Cell Separation / Mesenchymal Stem Cell Transplantation / Mesenchymal Stem Cells / Granulocytes Type of study: Diagnostic_studies / Evaluation_studies Limits: Humans / Male Language: En Journal: Biomed Mater Eng Year: 2008 Document type: Article