5-aminolaevulinic acid and photodynamic therapy reduce HSV-1 replication in HaCat cells through an apoptosis-independent mechanism.
Photodermatol Photoimmunol Photomed
; 24(5): 237-43, 2008 Oct.
Article
in En
| MEDLINE
| ID: mdl-18811864
ABSTRACT
BACKGROUND:
Photodynamic therapy (PDT) involves the use of a photosensitizing agent, which may require metabolic synthesis (i.e. a prodrug), followed by light activation. Numerous studies have advanced PDT as a means for treating bacteria, fungi and viruses. In this study, the photoinactivation of Herpes simplex virus type 1 (HSV-1) in human keratinocytes using 5-aminolaevulinic acid (5-ALA) was investigated.METHODS:
HaCat cells were infected with HSV-1 and treated with 5-ALA to verify its antiviral effect during the stages of adsorption and penetration to host cells. Immunoblot analysis was used to estimate the effect of ALA-PDT on the production of viral proteins glycoprotein D (gD), infected cell proteins (ICP) 27 and virion protein (VP) 16. We also investigated whether the effect of ALA-PDT was associated with a cellular apoptotic mechanism through DNA fragmentation and the study of p53, PARP and caspase-3 protein expression.RESULTS:
While the treatment of ALA-PDT after the viral adsorption period reduced HSV-1 replication by about 70%, it did not act on the virus in the first phase of infection. The viral proteins' expressions were reduced by ALA-PDT treatments. There was no evidence of ALA-PDT-induced apoptosis.CONCLUSION:
Our data suggest that the target of photoinactivation appears to be viral replication and not a cellular response.
Full text:
1
Collection:
01-internacional
Health context:
6_ODS3_enfermedades_notrasmisibles
Database:
MEDLINE
Main subject:
Photochemotherapy
/
Virus Replication
/
Prodrugs
/
Photosensitizing Agents
/
Apoptosis
/
Herpesvirus 1, Human
/
Aminolevulinic Acid
/
Herpes Simplex
Limits:
Humans
Language:
En
Journal:
Photodermatol Photoimmunol Photomed
Year:
2008
Document type:
Article