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On-chip detection of low-molecular-weight recombinant proteins in plant crude extracts by SELDI-TOF MS.
Badri, Amine M; Coenen, Karine; Vaillancourt, Louis-Philippe; Goulet, Charles; Michaud, Dominique.
Affiliation
  • Badri AM; Département de phytologie, Université Laval, Québec, Canada.
Methods Mol Biol ; 483: 313-24, 2009.
Article in En | MEDLINE | ID: mdl-19183907
This chapter presents a general procedure for the on-chip detection and quantitation of low-molecular-weight recombinant proteins in transgenic plant crude extracts by surface-enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF MS). A protocol is first described to detect the protein of interest in crude protein extracts of transgenic plant lines, by differential protein mapping against similar extracts from a control, nontransgenic line. A complementary protocol is then presented to generate a standard curve with the SELDI system, allowing the protein to be quantified in different transgenic lines. Overall, this procedure may be carried out within a few hours, without the need for prior purification or enrichment of the recombinant protein.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Plant Extracts / Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Type of study: Diagnostic_studies Language: En Journal: Methods Mol Biol Year: 2009 Document type: Article

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Plant Extracts / Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Type of study: Diagnostic_studies Language: En Journal: Methods Mol Biol Year: 2009 Document type: Article