Intracellular uptake of a new boronated porphyrin EC032.
Appl Radiat Isot
; 67(7-8 Suppl): S94-6, 2009 Jul.
Article
in En
| MEDLINE
| ID: mdl-19410469
ABSTRACT
We measured the toxicity and intracellular uptake of a newly developed boronated porphyrin EC032, and verified the fluorescence-based boron concentration measuring methods. Toxicity study showed that concentration required to produce a 50% reduction in viability (IC(50)) of EC032 was more than 0.25 mM. Fluorescence study showed the intracellular uptake of EC032 increased up until 24 h after its exposure to C6, 9L, U87, and U251 cells. There was also a linear correlation between ICP-AES and fluorescence intensity as an arbitrary unit about measurement of boron concentration. Fluorescence-based boron concentration measuring methods are very simple and useful methods, especially for screening of slight test dose of porphyrin compounds.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Radiation-Sensitizing Agents
/
Boron Compounds
/
Boron Neutron Capture Therapy
Limits:
Animals
/
Humans
Language:
En
Journal:
Appl Radiat Isot
Year:
2009
Document type:
Article